UniProt UniProt http://www.uniprot.org Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein-1 is a new member of the Cupin superfamily. A possible multifunctional protein acting as an invasion suppressor down-regulated in tumors. Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein-1 is a new member of the Cupin superfamily. A possible multifunctional protein acting as an invasion suppressor down-regulated in tumors. J. Biol. Chem. (0021-9258) 2004 imex curation Uekita T., Gotoh I., Kinoshita T., Itoh Y., Sato H., Shiomi T., Okada Y., Seiki M. BioCreative - Critical Assessment of Information Extraction systems in Biology human lung fibroblast cell (WI-38) cDNA library Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Uekita T., Gotoh I., Kinoshita T., Itoh Y., Sato H., Shiomi T., Okada Y., Seiki M. BioCreative - Critical Assessment of Information Extraction systems in Biology Accepted 2006-SEP-14 by DAVET. human lung fibroblast cell (WI-38) cDNA library J. Biol. Chem. (0021-9258) 2004 Only protein-protein interactions imex curation Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein-1 is a new member of the Cupin superfamily. A possible multifunctional protein acting as an invasion suppressor down-regulated in tumors. Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein-1 is a new member of the Cupin superfamily. A possible multifunctional protein acting as an invasion suppressor down-regulated in tumors. J. Biol. Chem. (0021-9258) 2004 imex curation Uekita T., Gotoh I., Kinoshita T., Itoh Y., Sato H., Shiomi T., Okada Y., Seiki M. BioCreative - Critical Assessment of Information Extraction systems in Biology human lung fibroblast cell (WI-38) cDNA library Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Uekita T., Gotoh I., Kinoshita T., Itoh Y., Sato H., Shiomi T., Okada Y., Seiki M. BioCreative - Critical Assessment of Information Extraction systems in Biology Accepted 2006-SEP-14 by DAVET. human lung fibroblast cell (WI-38) cDNA library J. Biol. Chem. (0021-9258) 2004 Only protein-protein interactions imex curation Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein-1 is a new member of the Cupin superfamily. A possible multifunctional protein acting as an invasion suppressor down-regulated in tumors. Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein-1 is a new member of the Cupin superfamily. A possible multifunctional protein acting as an invasion suppressor down-regulated in tumors. J. Biol. Chem. (0021-9258) 2004 imex curation Uekita T., Gotoh I., Kinoshita T., Itoh Y., Sato H., Shiomi T., Okada Y., Seiki M. BioCreative - Critical Assessment of Information Extraction systems in Biology human lung fibroblast cell (WI-38) cDNA library Only protein-protein interactions human-ht1080 Homo sapiens HT1080 fribrosarcoma cell line ht1080 confirmed as human with IEF of AST, LDH, NP established from the biopsy from a fibrosarcoma of a 35-year-old Caucasian man in 1972 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Uekita T., Gotoh I., Kinoshita T., Itoh Y., Sato H., Shiomi T., Okada Y., Seiki M. BioCreative - Critical Assessment of Information Extraction systems in Biology Human HT1080 cells habe been transiently trasnsfected with MMP14 and ADIL/MTCBP1. Accepted 2006-SEP-14 by DAVET. human lung fibroblast cell (WI-38) cDNA library J. Biol. Chem. (0021-9258) 2004 Only protein-protein interactions imex curation Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein-1 is a new member of the Cupin superfamily. A possible multifunctional protein acting as an invasion suppressor down-regulated in tumors. Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein-1 is a new member of the Cupin superfamily. A possible multifunctional protein acting as an invasion suppressor down-regulated in tumors. J. Biol. Chem. (0021-9258) 2004 imex curation Uekita T., Gotoh I., Kinoshita T., Itoh Y., Sato H., Shiomi T., Okada Y., Seiki M. BioCreative - Critical Assessment of Information Extraction systems in Biology human lung fibroblast cell (WI-38) cDNA library Only protein-protein interactions human-ht1080 Homo sapiens HT1080 fribrosarcoma cell line ht1080 confirmed as human with IEF of AST, LDH, NP established from the biopsy from a fibrosarcoma of a 35-year-old Caucasian man in 1972 confocal microscopy confocal microscopy antibody detection identification by antibody antibody detection Uekita T., Gotoh I., Kinoshita T., Itoh Y., Sato H., Shiomi T., Okada Y., Seiki M. BioCreative - Critical Assessment of Information Extraction systems in Biology Human HT1080 cells habe been transiently trasnsfected with MMP14 and ADIL/MTCBP1. Accepted 2006-SEP-14 by DAVET. human lung fibroblast cell (WI-38) cDNA library J. Biol. Chem. (0021-9258) 2004 Only protein-protein interactions imex curation mmp14_human Matrix metalloproteinase-14 Membrane-type matrix metalloproteinase 1 Membrane-type-1 matrix metalloproteinase MMP-X1 MMP14 protein protein human Homo sapiens Human MSPAPRPPRCLLLPLLTLGTALASLGSAQSSSFSPEAWLQQYGYLPPGDLRTHTQRSPQSLSAAIAAMQKFYGLQVTGKADADTMKAMRRPRCGVPDKFGAEIKANVRRKRYAIQGLKWQHNEITFCIQNYTPKVGEYATYEAIRKAFRVWESATPLRFREVPYAYIREGHEKQADIMIFFAEGFHGDSTPFDGEGGFLAHAYFPGPNIGGDTHFDSAEPWTVRNEDLNGNDIFLVAVHELGHALGLEHSSDPSAIMAPFYQWMDTENFVLPDDDRRGIQQLYGGESGFPTKMPPQPRTTSRPSVPDKPKNPTYGPNICDGNFDTVAMLRGEMFVFKERWFWRVRNNQVMDGYPMPIGQFWRGLPASINTAYERKDGKFVFFKGDKHWVFDEASLEPGYPKHIKELGRGLPTDKIDAALFWMPNGKTYFFRGNKYYRFNEELRAVDSEYPKNIKVWEGIPESPRGSFMGSDEVFTYFYKGNKYWKFNNQKLKVEPGYPKSALRDWMGCPSGGRPDEGTEEETEVIIIEVDEEGGGAVSAAAVVLPVLLLLLVLAVGLAVFFFRRHGTPRRLLYCQRSLLDKV DIP protein P50281 original sequence version: 121 3722DE286A4BBCDE mtnd_human 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1 ADI1 MTCBP1 Acireductone dioxygenase (Fe(2+)-requiring) Submergence-induced protein-like factor HMFT1638 protein protein human Homo sapiens Human MVQAWYMDDAPGDPRQPHRPDPGRPVGLEQLRRLGVLYWKLDADKYENDPELEKIRRERNYSWMDIITICKDKLPNYEEKIKMFYEEHLHLDDEIRYILDGSGYFDVRDKEDQWIRIFMEKGDMVTLPAGIYHRFTVDEKNYTKAMRLFVGEPVWTAYNRPADHFEARGQYVKFLAQTA 92E13B9718D44C27 mmp14-adi1-1 1 6 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 5 unspecified role unspecified role bait bait cytoplasmic region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association Thus, these two cDNAs are presumably derived from transcripts of a single gene that has multiple transcriptional termination sites. Both transcripts have one open reading frame that potentially encodes a 179-amino acid polypeptide. Because the product of the gene is expected to bind to the cytoplasmic tail of MT1-MMP, it was none mmp14-adi1-2 2 5 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cytolasmic region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro 6 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) physical association physical association 3A MTCBP-1 expressed in cells formed a complex with MT1-MMP and co-localized at the membrane. mmp14-adi1-3 3 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cytolasmic region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association 3B MTCBP-1 expressed in cells formed a complex with MT1-MMP and co-localized at the membrane. mmp14-adi1-4 4 5 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role neutral component neutral component colocalization colocalization 3C MTCBP-1 expressed in cells formed a complex with MT1-MMP and co-localized at the membrane.