IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Crystal structure of TNF-alpha mutant R31D with greater affinity for receptor R1 compared with R2. Crystal structure of TNF-alpha mutant R31D with greater affinity for receptor R1 compared with R2. Protein Eng. (0269-2139) 1997 imex curation Reed C., Fu ZQ., Wu J., Xue YN., Harrison RW., Chen MJ., Weber IT. Apoptosis - Interactions involving proteins with a function related to apoptosis orchard Only protein-protein interactions Curated by: I2D - Interologous Interaction Database in vitro In vitro solid phase assay solid phase assay tag visualisation tag visualisation Reed C., Fu ZQ., Wu J., Xue YN., Harrison RW., Chen MJ., Weber IT. Apoptosis - Interactions involving proteins with a function related to apoptosis Accepted 2010-SEP-17 by ORCHARD. Protein Eng. (0269-2139) 1997 orchard Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Crystal structure of TNF-alpha mutant R31D with greater affinity for receptor R1 compared with R2. Crystal structure of TNF-alpha mutant R31D with greater affinity for receptor R1 compared with R2. Protein Eng. (0269-2139) 1997 imex curation Reed C., Fu ZQ., Wu J., Xue YN., Harrison RW., Chen MJ., Weber IT. Apoptosis - Interactions involving proteins with a function related to apoptosis orchard Only protein-protein interactions Curated by: I2D - Interologous Interaction Database in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Reed C., Fu ZQ., Wu J., Xue YN., Harrison RW., Chen MJ., Weber IT. Apoptosis - Interactions involving proteins with a function related to apoptosis Accepted 2010-SEP-17 by ORCHARD. Protein Eng. (0269-2139) 1997 orchard Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database p01375-pro_0000034424 Tumor necrosis factor, soluble form TNF TNFA TNFSF2 Cachectin TNF-alpha Tumor necrosis factor ligand superfamily member 2 protein protein human Homo sapiens Human VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL 77 233 D134511F90BCCF20 tnr1b_human Tumor necrosis factor receptor superfamily member 1B TNFRSF1B TNFBR TNFR2 Tumor necrosis factor receptor 2 Tumor necrosis factor receptor type II p75 p80 TNF-alpha receptor protein protein human Homo sapiens Human MAPVAVWAALAVGLELWAAAHALPAQVAFTPYAPEPGSTCRLREYYDQTAQMCCSKCSPGQHAKVFCTKTSDTVCDSCEDSTYTQLWNWVPECLSCGSRCSSDQVETQACTREQNRICTCRPGWYCALSKQEGCRLCAPLRKCRPGFGVARPGTETSDVVCKPCAPGTFSNTTSSTDICRPHQICNVVAIPGNASMDAVCTSTSPTRSMAPGAVHLPQPVSTRSQHTQPTPEPSTAPSTSFLLPMGPSPPAEGSTGDFALPVGLIVGVTALGLLIIGVVNCVIMTQVKKKPLCLQREAKVPHLPADKARGTQGPEQQHLLITAPSSSSSSLESSASALDRRAPTRNQPQAPGVEASGAGEARASTGSSDSSPGGHGTQVNVTCIVNVCSSSDHSSQCSSQASSTMGDTDSSPSESPKDEQVPFSKEECAFRSQLETPETLLGSTEEKPLPLGVPDAGMKPS 603D0AE1CD69ACBF tnr1a_human Tumor necrosis factor receptor superfamily member 1A TNFRSF1A TNFAR TNFR1 p60 Tumor necrosis factor receptor 1 Tumor necrosis factor receptor type I p55 protein protein human Homo sapiens Human MGLSTVPDLLLPLVLLELLVGIYPSGVIGLVPHLGDREKRDSVCPQGKYIHPQNNSICCTKCHKGTYLYNDCPGPGQDTDCRECESGSFTASENHLRHCLSCSKCRKEMGQVEISSCTVDRDTVCGCRKNQYRHYWSENLFQCFNCSLCLNGTVHLSCQEKQNTVCTCHAGFFLRENECVSCSNCKKSLECTKLCLPQIENVKGTEDSGTTVLLPLVIFFGLCLLSLLFIGLMYRYQRWKSKLYSIVCGKSTPEKEGELEGTTTKPLAPNPSFSPTPGFTPTLGFSPVPSSTFTSSSTYTPGDCPNFAAPRREVAPPYQGADPILATALASDPIPNPLQKWEDSAHKPQSLDTDDPATLYAVVENVPPLRWKEFVRRLGLSDHEIDRLELQNGRCLREAQYSMLATWRRRTPRREATLELLGRVLRDMDLLGCLEDIEEALCGPAALPPAPSLLR DIP protein P19438 original sequence version: 135 DIP protein P19438 original sequence version: 150 DIP protein P19438 original sequence version: 177 4CEFBA96D03B8225 tnfa 2 3 unspecified role unspecified role neutral component neutral component P01375-PRO_0000034424:p.Arg31Asp TNF R31D mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R D Wed May 02 14:57:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg31asp' Tue Jun 30 19:48:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg31Asp' ecoli Escherichia coli (strain K12) direct interaction direct interaction TNF R31D 2.3A TNF R31D R-working:21.8% R-free:24.0% Wild type TNF also crystallized but no reference is available in PDB tnfa-tnfr1 1 5 unspecified role unspecified role bait bait spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 3 unspecified role unspecified role prey prey region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P01375-PRO_0000034424:p.Arg31Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R D Thu Jul 07 12:15:38 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was arg31asp Wed May 02 20:32:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg31asp' Tue Jun 30 18:16:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg31Asp' ecoli Escherichia coli (strain K12) direct interaction direct interaction Table I tnfa-tnfr2-1 1 4 unspecified role unspecified role bait bait spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 3 unspecified role unspecified role prey prey region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P01375-PRO_0000034424:p.Arg31Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R D Thu Jul 07 12:15:38 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was arg31asp Wed May 02 18:27:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg31asp' Tue Jun 30 18:13:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg31Asp' ecoli Escherichia coli (strain K12) direct interaction direct interaction Table I