IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Leukemia-associated retinoic acid receptor alpha fusion partners, PML and PLZF, heterodimerize and colocalize to nuclear bodies. Leukemia-associated retinoic acid receptor alpha fusion partners, PML and PLZF, heterodimerize and colocalize to nuclear bodies. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 imex curation Koken MH., Reid A., Quignon F., Chelbi-Alix MK., Davies JM., Kabarowski JH., Zhu J., Dong S., Chen S., Chen Z., Tan CC., Licht J., Waxman S., de The H., Zelent A. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Proteins were transiently coexpressed in Cos6 cells, then after 18h labeled with 35S methionine:cysteine 2A arthur@icr.ac.uk Only protein-protein interactions human-kg1 Human Caucasian bone marrow myelogenous leukaemia kg1 Human Caucasian bone marrow myelogenous leukaemia imaging technique imaging technique microscopy immunostaining immunostaining Koken MH., Reid A., Quignon F., Chelbi-Alix MK., Davies JM., Kabarowski JH., Zhu J., Dong S., Chen S., Chen Z., Tan CC., Licht J., Waxman S., de The H., Zelent A. Apoptosis - Interactions involving proteins with a function related to apoptosis Proteins were transiently coexpressed in Cos6 cells, then after 18h labeled with 35S methionine:cysteine 2A arthur@icr.ac.uk Annotation to indicate that this entry has been checked and accepted by a senior curator. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 Only protein-protein interactions imex curation Leukemia-associated retinoic acid receptor alpha fusion partners, PML and PLZF, heterodimerize and colocalize to nuclear bodies. Leukemia-associated retinoic acid receptor alpha fusion partners, PML and PLZF, heterodimerize and colocalize to nuclear bodies. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 imex curation Koken MH., Reid A., Quignon F., Chelbi-Alix MK., Davies JM., Kabarowski JH., Zhu J., Dong S., Chen S., Chen Z., Tan CC., Licht J., Waxman S., de The H., Zelent A. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Proteins were transiently coexpressed in Cos6 cells, then after 18h labeled with 35S methionine:cysteine 2A arthur@icr.ac.uk Only protein-protein interactions chlae-cos_6 African green monkey kidney cell line Grivet cos_6 African green monkey kidney cell line imaging technique imaging technique microscopy immunostaining immunostaining Koken MH., Reid A., Quignon F., Chelbi-Alix MK., Davies JM., Kabarowski JH., Zhu J., Dong S., Chen S., Chen Z., Tan CC., Licht J., Waxman S., de The H., Zelent A. Apoptosis - Interactions involving proteins with a function related to apoptosis Proteins were transiently coexpressed in Cos6 cells, then after 18h labeled with 35S methionine:cysteine 2A arthur@icr.ac.uk Annotation to indicate that this entry has been checked and accepted by a senior curator. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 Only protein-protein interactions imex curation Leukemia-associated retinoic acid receptor alpha fusion partners, PML and PLZF, heterodimerize and colocalize to nuclear bodies. Leukemia-associated retinoic acid receptor alpha fusion partners, PML and PLZF, heterodimerize and colocalize to nuclear bodies. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 imex curation Koken MH., Reid A., Quignon F., Chelbi-Alix MK., Davies JM., Kabarowski JH., Zhu J., Dong S., Chen S., Chen Z., Tan CC., Licht J., Waxman S., de The H., Zelent A. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Proteins were transiently coexpressed in Cos6 cells, then after 18h labeled with 35S methionine:cysteine 2A arthur@icr.ac.uk Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Koken MH., Reid A., Quignon F., Chelbi-Alix MK., Davies JM., Kabarowski JH., Zhu J., Dong S., Chen S., Chen Z., Tan CC., Licht J., Waxman S., de The H., Zelent A. Apoptosis - Interactions involving proteins with a function related to apoptosis Proteins were transiently coexpressed in Cos6 cells, then after 18h labeled with 35S methionine:cysteine 2A arthur@icr.ac.uk Annotation to indicate that this entry has been checked and accepted by a senior curator. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 Only protein-protein interactions imex curation Leukemia-associated retinoic acid receptor alpha fusion partners, PML and PLZF, heterodimerize and colocalize to nuclear bodies. Leukemia-associated retinoic acid receptor alpha fusion partners, PML and PLZF, heterodimerize and colocalize to nuclear bodies. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 imex curation Koken MH., Reid A., Quignon F., Chelbi-Alix MK., Davies JM., Kabarowski JH., Zhu J., Dong S., Chen S., Chen Z., Tan CC., Licht J., Waxman S., de The H., Zelent A. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Proteins were transiently coexpressed in Cos6 cells, then after 18h labeled with 35S methionine:cysteine 2A arthur@icr.ac.uk Only protein-protein interactions chlae-cos_6 African green monkey kidney cell line Grivet cos_6 African green monkey kidney cell line anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Koken MH., Reid A., Quignon F., Chelbi-Alix MK., Davies JM., Kabarowski JH., Zhu J., Dong S., Chen S., Chen Z., Tan CC., Licht J., Waxman S., de The H., Zelent A. Apoptosis - Interactions involving proteins with a function related to apoptosis Proteins were transiently coexpressed in Cos6 cells, then after 18h labeled with 35S methionine:cysteine 2A arthur@icr.ac.uk Annotation to indicate that this entry has been checked and accepted by a senior curator. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 Only protein-protein interactions imex curation Leukemia-associated retinoic acid receptor alpha fusion partners, PML and PLZF, heterodimerize and colocalize to nuclear bodies. Leukemia-associated retinoic acid receptor alpha fusion partners, PML and PLZF, heterodimerize and colocalize to nuclear bodies. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 imex curation Koken MH., Reid A., Quignon F., Chelbi-Alix MK., Davies JM., Kabarowski JH., Zhu J., Dong S., Chen S., Chen Z., Tan CC., Licht J., Waxman S., de The H., Zelent A. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Proteins were transiently coexpressed in Cos6 cells, then after 18h labeled with 35S methionine:cysteine 2A arthur@icr.ac.uk Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Koken MH., Reid A., Quignon F., Chelbi-Alix MK., Davies JM., Kabarowski JH., Zhu J., Dong S., Chen S., Chen Z., Tan CC., Licht J., Waxman S., de The H., Zelent A. Apoptosis - Interactions involving proteins with a function related to apoptosis Proteins were transiently coexpressed in Cos6 cells, then after 18h labeled with 35S methionine:cysteine 2A arthur@icr.ac.uk Annotation to indicate that this entry has been checked and accepted by a senior curator. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 Only protein-protein interactions imex curation zbt16_human Zinc finger and BTB domain-containing protein 16 Zinc finger protein PLZF Promyelocytic leukemia zinc finger protein Zinc finger protein 145 ZBTB16 PLZF ZNF145 protein protein human Homo sapiens Human MDLTKMGMIQLQNPSHPTGLLCKANQMRLAGTLCDVVIMVDSQEFHAHRTVLACTSKMFEILFHRNSQHYTLDFLSPKTFQQILEYAYTATLQAKAEDLDDLLYAAEILEIEYLEEQCLKMLETIQASDDNDTEATMADGGAEEEEDRKARYLKNIFISKHSSEESGYASVAGQSLPGPMVDQSPSVSTSFGLSAMSPTKAAVDSLMTIGQSLLQGTLQPPAGPEEPTLAGGGRHPGVAEVKTEMMQVDEVPSQDSPGAAESSISGGMGDKVEERGKEGPGTPTRSSVITSARELHYGREESAEQVPPPAEAGQAPTGRPEHPAPPPEKHLGIYSVLPNHKADAVLSMPSSVTSGLHVQPALAVSMDFSTYGGLLPQGFIQRELFSKLGELAVGMKSESRTIGEQCSVCGVELPDNEAVEQHRKLHSGMKTYGCELCGKRFLDSLRLRMHLLAHSAGAKAFVCDQCGAQFSKEDALETHRQTHTGTDMAVFCLLCGKRFQAQSALQQHMEVHAGVRSYICSECNRTFPSHTALKRHLRSHTGDHPYECEFCGSCFRDESTLKSHKRIHTGEKPYECNGCGKKFSLKHQLETHYRVHTGEKPFECKLCHQRSRDYSAMIKHLRTHNGASPYQCTICTEYCPSLSSMQKHMKGHKPEEIPPDWRIEKTYLYLCYV 51F8E361FA31239E pml_human Protein PML PML MYL RNF71 TRIM19 Tripartite motif-containing protein 19 RING finger protein 71 Promyelocytic leukemia protein PP8675 E3 SUMO-protein ligase PML RING-type E3 SUMO transferase PML protein protein human Homo sapiens Human MEPAPARSPRPQQDPARPQEPTMPPPETPSEGRQPSPSPSPTERAPASEEEFQFLRCQQCQAEAKCPKLLPCLHTLCSGCLEASGMQCPICQAPWPLGADTPALDNVFFESLQRRLSVYRQIVDAQAVCTRCKESADFWCFECEQLLCAKCFEAHQWFLKHEARPLAELRNQSVREFLDGTRKTNNIFCSNPNHRTPTLTSIYCRGCSKPLCCSCALLDSSHSELKCDISAEIQQRQEELDAMTQALQEQDSAFGAVHAQMHAAVGQLGRARAETEELIRERVRQVVAHVRAQERELLEAVDARYQRDYEEMASRLGRLDAVLQRIRTGSALVQRMKCYASDQEVLDMHGFLRQALCRLRQEEPQSLQAAVRTDGFDEFKVRLQDLSSCITQGKDAAVSKKASPEAASTPRDPIDVDLPEEAERVKAQVQALGLAEAQPMAVVQSVPGAHPVPVYAFSIKGPSYGEDVSNTTTAQKRKCSQTQCPRKVIKMESEEGKEARLARSSPEQPRPSTSKAVSPPHLDGPPSPRSPVIGSEVFLPNSNHVASGAGEAEERVVVISSSEDSDAENSSSRELDDSSSESSDLQLEGPSTLRVLDENLADPQAEDRPLVFFDLKIDNETQKISQLAAVNRESKFRVVIQPEAFFSIYSKAVSLEVGLQHFLSFLSSMRRPILACYKLWGPGLPNFFRALEDINRLWEFQEAISGFLAALPLIRERVPGASSFKLKNLAQTYLARNMSERSAMAAVLAMRDLCRLLEVSPGPQLAQHVYPFSSLQCFASLQPLVQAAVLPRAEARLLALHNVSFMELLSAHRRDRQGGLKKYSRYLSLQTTTLPPAQPAFNLQALGTYFEGLLEGPALARAEGVSTPLAGRGLAERASQQS DIP protein P29590 original sequence version: 188 DIP protein P29590 original sequence version: 195 DIP protein P29590-1 original sequence version: 187 D50968A977E34287 p29590-5 Protein PML PML MYL RNF71 TRIM19 RING finger protein 71 Tripartite motif-containing protein 19 PML-IV PML-X TRIM19zeta Promyelocytic leukemia protein PP8675 E3 SUMO-protein ligase PML RING-type E3 SUMO transferase PML protein protein human Homo sapiens Human MEPAPARSPRPQQDPARPQEPTMPPPETPSEGRQPSPSPSPTERAPASEEEFQFLRCQQCQAEAKCPKLLPCLHTLCSGCLEASGMQCPICQAPWPLGADTPALDNVFFESLQRRLSVYRQIVDAQAVCTRCKESADFWCFECEQLLCAKCFEAHQWFLKHEARPLAELRNQSVREFLDGTRKTNNIFCSNPNHRTPTLTSIYCRGCSKPLCCSCALLDSSHSELKCDISAEIQQRQEELDAMTQALQEQDSAFGAVHAQMHAAVGQLGRARAETEELIRERVRQVVAHVRAQERELLEAVDARYQRDYEEMASRLGRLDAVLQRIRTGSALVQRMKCYASDQEVLDMHGFLRQALCRLRQEEPQSLQAAVRTDGFDEFKVRLQDLSSCITQGKDAAVSKKASPEAASTPRDPIDVDLPEEAERVKAQVQALGLAEAQPMAVVQSVPGAHPVPVYAFSIKGPSYGEDVSNTTTAQKRKCSQTQCPRKVIKMESEEGKEARLARSSPEQPRPSTSKAVSPPHLDGPPSPRSPVIGSEVFLPNSNHVASGAGEAEERVVVISSSEDSDAENSSSRELDDSSSESSDLQLEGPSTLRVLDENLADPQAEDRPLVFFDLKIDNESGFSWGYPHPFLI DIP protein P29590-5 original sequence version: 188 DIP protein P29590-5 original sequence version: 195 85FBAEC9F162C8E0 plzf-pml-1 4 7 unspecified role unspecified role prey prey 35s 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_6 African green monkey kidney cell line Grivet cos_6 African green monkey kidney cell line 6 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_6 African green monkey kidney cell line Grivet cos_6 African green monkey kidney cell line physical association physical association The complex was 97kD in size plzf-pml-2 3 8 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction plzf-pml-3 3 7 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction plzf-pml-4 3 7 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pml stop 381 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction plzf-plzf-1 3 6 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction plzf-pml-5 5 6 unspecified role unspecified role bait bait in vitro In vitro 7 unspecified role unspecified role prey prey pml d1 PML delta 1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro physical association physical association plzf-pml-6 5 8 unspecified role unspecified role prey prey in vitro In vitro 6 unspecified role unspecified role bait bait in vitro In vitro physical association physical association plzf-pml-7 5 6 unspecified role unspecified role bait bait in vitro In vitro 7 unspecified role unspecified role prey prey in vitro In vitro physical association physical association plzf-plzf-2 5 6 unspecified role unspecified role prey prey in vitro In vitro 6 unspecified role unspecified role bait bait in vitro In vitro physical association physical association pml-pml_x-1 5 8 unspecified role unspecified role prey prey in vitro In vitro 7 unspecified role unspecified role bait bait in vitro In vitro physical association physical association pml-plzf-1 2 6 unspecified role unspecified role neutral component neutral component chlae-cos_6 African green monkey kidney cell line Grivet cos_6 African green monkey kidney cell line 7 unspecified role unspecified role neutral component neutral component chlae-cos_6 African green monkey kidney cell line Grivet cos_6 African green monkey kidney cell line colocalization colocalization pml-plzf-2 1 6 unspecified role unspecified role neutral component neutral component human-kg1 Human Caucasian bone marrow myelogenous leukaemia kg1 Human Caucasian bone marrow myelogenous leukaemia 7 unspecified role unspecified role neutral component neutral component human-kg1 Human Caucasian bone marrow myelogenous leukaemia kg1 Human Caucasian bone marrow myelogenous leukaemia colocalization colocalization