IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ BAP-135, a target for Bruton's tyrosine kinase in response to B cell receptor engagement. BAP-135, a target for Bruton's tyrosine kinase in response to B cell receptor engagement. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 Yang W., Desiderio S. Apoptosis - Interactions involving proteins with a function related to apoptosis 1 human-ramos Human Caucasian Burkitt's lymphoma ramos Human Caucasian Burkitt's lymphoma coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Yang W., Desiderio S. Apoptosis - Interactions involving proteins with a function related to apoptosis 1 Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 BAP-135, a target for Bruton's tyrosine kinase in response to B cell receptor engagement. BAP-135, a target for Bruton's tyrosine kinase in response to B cell receptor engagement. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 Yang W., Desiderio S. Apoptosis - Interactions involving proteins with a function related to apoptosis 1 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Yang W., Desiderio S. Apoptosis - Interactions involving proteins with a function related to apoptosis 1 Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 BAP-135, a target for Bruton's tyrosine kinase in response to B cell receptor engagement. BAP-135, a target for Bruton's tyrosine kinase in response to B cell receptor engagement. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 Yang W., Desiderio S. Apoptosis - Interactions involving proteins with a function related to apoptosis 1 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Yang W., Desiderio S. Apoptosis - Interactions involving proteins with a function related to apoptosis Total cell lysates were fractionated by SDS/PAGE, and phosphotyrosine-containing proteins were detected by immunoblotting with antibody 4G10. The blot was then reprobed with protein-specific antibodies. 1 Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 BAP-135, a target for Bruton's tyrosine kinase in response to B cell receptor engagement. BAP-135, a target for Bruton's tyrosine kinase in response to B cell receptor engagement. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 Yang W., Desiderio S. Apoptosis - Interactions involving proteins with a function related to apoptosis 1 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Yang W., Desiderio S. Apoptosis - Interactions involving proteins with a function related to apoptosis 1 Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 BAP-135, a target for Bruton's tyrosine kinase in response to B cell receptor engagement. BAP-135, a target for Bruton's tyrosine kinase in response to B cell receptor engagement. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 Yang W., Desiderio S. Apoptosis - Interactions involving proteins with a function related to apoptosis 1 human-ramos Human Caucasian Burkitt's lymphoma ramos Human Caucasian Burkitt's lymphoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yang W., Desiderio S. Apoptosis - Interactions involving proteins with a function related to apoptosis 1 Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 btk_mouse Tyrosine-protein kinase BTK Bruton tyrosine kinase B-cell progenitor kinase Kinase EMB Btk Bpk Agammaglobulinemia tyrosine kinase protein protein mouse Mus musculus Mouse MAAVILESIFLKRSQQKKKTSPLNFKKRLFLLTVHKLSYYEYDFERGRRGSKKGSIDVEKITCVETVIPEKNPPPERQIPRRGEESSEMEQISIIERFPYPFQVVYDEGPLYVFSPTEELRKRWIHQLKNVIRYNSDLVQKYHPCFWIDGQYLCCSQTAKNAMGCQILENRNGSLKPGSSHRKTKKPLPPTPEEDQILKKPLPPEPTAAPISTTELKKVVALYDYMPMNANDLQLRKGEEYFILEESNLPWWRARDKNGQEGYIPSNYITEAEDSIEMYEWYSKHMTRSQAEQLLKQEGKEGGFIVRDSSKAGKYTVSVFAKSTGEPQGVIRHYVVCSTPQSQYYLAEKHLFSTIPELINYHQHNSAGLISRLKYPVSKQNKNAPSTAGLGYGSWEIDPKDLTFLKELGTGQFGVVKYGKWRGQYDVAIKMIREGSMSEDEFIEEAKVMMNLSHEKLVQLYGVCTKQRPIFIITEYMANGCLLNYLREMRHRFQTQQLLEMCKDVCEAMEYLESKQFLHRDLAARNCLVNDQGVVKVSDFGLSRYVLDDEYTSSVGSKFPVRWSPPEVLMYSKFSSKSDIWAFGVLMWEIYSLGKMPYERFTNSETAEHIAQGLRLYRPHLASERVYTIMYSCWHEKADERPSFKILLSNILDVMDEES E502B798BC36E223 btk_human Tyrosine-protein kinase BTK Bruton tyrosine kinase B-cell progenitor kinase BPK BTK AGMX1 ATK Agammaglobulinemia tyrosine kinase protein protein human Homo sapiens Human MAAVILESIFLKRSQQKKKTSPLNFKKRLFLLTVHKLSYYEYDFERGRRGSKKGSIDVEKITCVETVVPEKNPPPERQIPRRGEESSEMEQISIIERFPYPFQVVYDEGPLYVFSPTEELRKRWIHQLKNVIRYNSDLVQKYHPCFWIDGQYLCCSQTAKNAMGCQILENRNGSLKPGSSHRKTKKPLPPTPEEDQILKKPLPPEPAAAPVSTSELKKVVALYDYMPMNANDLQLRKGDEYFILEESNLPWWRARDKNGQEGYIPSNYVTEAEDSIEMYEWYSKHMTRSQAEQLLKQEGKEGGFIVRDSSKAGKYTVSVFAKSTGDPQGVIRHYVVCSTPQSQYYLAEKHLFSTIPELINYHQHNSAGLISRLKYPVSQQNKNAPSTAGLGYGSWEIDPKDLTFLKELGTGQFGVVKYGKWRGQYDVAIKMIKEGSMSEDEFIEEAKVMMNLSHEKLVQLYGVCTKQRPIFIITEYMANGCLLNYLREMRHRFQTQQLLEMCKDVCEAMEYLESKQFLHRDLAARNCLVNDQGVVKVSDFGLSRYVLDDEYTSSVGSKFPVRWSPPEVLMYSKFSSKSDIWAFGVLMWEIYSLGKMPYERFTNSETAEHIAQGLRLYRPHLASEKVYTIMYSCWHEKADERPTFKILLSNILDVMDEES DIP protein Q06187 original sequence version: 118 DIP protein Q06187 original sequence version: 143 DF06B5D1FEC257CC gtf2i_human General transcription factor II-I GTF2I BAP135 WBSCR6 Bruton tyrosine kinase-associated protein 135 SRF-Phox1-interacting protein Williams-Beuren syndrome chromosomal region 6 protein protein protein human Homo sapiens Human MAQVAMSTLPVEDEESSESRMVVTFLMSALESMCKELAKSKAEVACIAVYETDVFVVGTERGRAFVNTRKDFQKDFVKYCVEEEEKAAEMHKMKSTTQANRMSVDAVEIETLRKTVEDYFCFCYGKALGKSTVVPVPYEKMLRDQSAVVVQGLPEGVAFKHPENYDLATLKWILENKAGISFIIKRPFLEPKKHVGGRVMVTDADRSILSPGGSCGPIKVKTEPTEDSGISLEMAAVTVKEESEDPDYYQYNIQAGPSETDDVDEKQPLSKPLQGSHHSSEGNEGTEMEVPAEDSTQHVPSETSEDPEVEVTIEDDDYSPPSKRPKANELPQPPVPEPANAGKRKVREFNFEKWNARITDLRKQVEELFERKYAQAIKAKGPVTIPYPLFQSHVEDLYVEGLPEGIPFRRPSTYGIPRLERILLAKERIRFVIKKHELLNSTREDLQLDKPASGVKEEWYARITKLRKMVDQLFCKKFAEALGSTEAKAVPYQKFEAHPNDLYVEGLPENIPFRSPSWYGIPRLEKIIQVGNRIKFVIKRPELLTHSTTEVTQPRTNTPVKEDWNVRITKLRKQVEEIFNLKFAQALGLTEAVKVPYPVFESNPEFLYVEGLPEGIPFRSPTWFGIPRLERIVRGSNKIKFVVKKPELVISYLPPGMASKINTKALQSPKRPRSPGSNSKVPEIEVTVEGPNNNNPQTSAVRTPTQTNGSNVPFKPRGREFSFEAWNAKITDLKQKVENLFNEKCGEALGLKQAVKVPFALFESFPEDFYVEGLPEGVPFRRPSTFGIPRLEKILRNKAKIKFIIKKPEMFETAIKESTSSKSPPRKINSSPNVNTTASGVEDLNIIQVTIPDDDNERLSKVEKARQLREQVNDLFSRKFGEAIGMGFPVKVPYRKITINPGCVVVDGMPPGVSFKAPSYLEISSMRRILDSAEFIKFTVIRPFPGLVINNQLVDQSESEGPVIQESAEPSQLEVPATEEIKETDGSSQIKQEPDPTW 4CFA2C19002869B9 btk-gtf21-1 1 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association Pervanadate - stimulates the kinase activity of BTK rather than the interaction. btk-gtf21-2 5 8 unspecified role unspecified role prey prey region (potential) phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait physical association physical association Pervanadate - stimulates the kinase activity of BTK rather than the interaction. btk-gtf21-3 5 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Interaction occurs if BTK is kinase-dead (Mutant K430E) btk-gtf21-4 2 8 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey physical association physical association Interaction occurs if BTK is kinase-dead (Mutant K430E) btk-gtf21-5 3 8 enzyme target enzyme target substrate substrate unspecified role unspecified role region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 enzyme enzyme unspecified role unspecified role Q06187:p.Tyr223Phe mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 15:22:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr223phe' Tue Jun 30 17:23:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr223Phe' Q06187:p.Tyr551Phe mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 17:41:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr551phe' Tue Jun 30 15:47:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr551Phe' Q06187:p.Arg307Lys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R K Wed May 02 15:30:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg307lys' Tue Jun 30 17:33:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg307Lys' phosphorylation phosphorylation reaction phosphorylation Phosphorylation of GTF2I (BAP-135) btk-gtf21-6 4 8 unspecified role unspecified role prey prey human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 7 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Interaction occurs if BTK is kinase-dead (Mutant K430E)