InnateDB InnateDB Caspase-12 controls West Nile virus infection via the viral RNA receptor RIG-I. Caspase-12 controls West Nile virus infection via the viral RNA receptor RIG-I. Nat. Immunol. (1529-2908) 2010 imex curation Wang P., Arjona A., Zhang Y., Sultana H., Dai J., Yang L., LeBlanc PM., Doiron K., Saleh M., Fikrig E. Apoptosis - Interactions involving proteins with a function related to apoptosis TRIM25 detected via anti-tag western blot maya.saleh@mcgill.ca species of caspase-12 determined via correspondence orchard Only protein-protein interactions Curated by: InnateDB - a knowledge resource for innate immunity mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. pull down pull down western blot western blot Immuno blot Immuno blot Wang P., Arjona A., Zhang Y., Sultana H., Dai J., Yang L., LeBlanc PM., Doiron K., Saleh M., Fikrig E. Apoptosis - Interactions involving proteins with a function related to apoptosis TRIM25 detected via anti-tag western blot maya.saleh@mcgill.ca species of caspase-12 determined via correspondence Accepted 2011-APR-04 by ORCHARD Nat. Immunol. (1529-2908) 2010 orchard Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity Caspase-12 controls West Nile virus infection via the viral RNA receptor RIG-I. Caspase-12 controls West Nile virus infection via the viral RNA receptor RIG-I. Nat. Immunol. (1529-2908) 2010 imex curation Wang P., Arjona A., Zhang Y., Sultana H., Dai J., Yang L., LeBlanc PM., Doiron K., Saleh M., Fikrig E. Apoptosis - Interactions involving proteins with a function related to apoptosis TRIM25 detected via anti-tag western blot maya.saleh@mcgill.ca species of caspase-12 determined via correspondence orchard Only protein-protein interactions Curated by: InnateDB - a knowledge resource for innate immunity mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Wang P., Arjona A., Zhang Y., Sultana H., Dai J., Yang L., LeBlanc PM., Doiron K., Saleh M., Fikrig E. Apoptosis - Interactions involving proteins with a function related to apoptosis TRIM25 detected via anti-tag western blot RIG-I was detected via immunostaining maya.saleh@mcgill.ca species of caspase-12 determined via correspondence Accepted 2011-APR-04 by ORCHARD Nat. Immunol. (1529-2908) 2010 orchard Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity Caspase-12 controls West Nile virus infection via the viral RNA receptor RIG-I. Caspase-12 controls West Nile virus infection via the viral RNA receptor RIG-I. Nat. Immunol. (1529-2908) 2010 imex curation Wang P., Arjona A., Zhang Y., Sultana H., Dai J., Yang L., LeBlanc PM., Doiron K., Saleh M., Fikrig E. Apoptosis - Interactions involving proteins with a function related to apoptosis TRIM25 detected via anti-tag western blot maya.saleh@mcgill.ca species of caspase-12 determined via correspondence orchard Only protein-protein interactions Curated by: InnateDB - a knowledge resource for innate immunity in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Wang P., Arjona A., Zhang Y., Sultana H., Dai J., Yang L., LeBlanc PM., Doiron K., Saleh M., Fikrig E. Apoptosis - Interactions involving proteins with a function related to apoptosis TRIM25 detected via anti-tag western blot maya.saleh@mcgill.ca species of caspase-12 determined via correspondence Accepted 2011-APR-04 by ORCHARD Nat. Immunol. (1529-2908) 2010 orchard Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity Caspase-12 controls West Nile virus infection via the viral RNA receptor RIG-I. Caspase-12 controls West Nile virus infection via the viral RNA receptor RIG-I. Nat. Immunol. (1529-2908) 2010 imex curation Wang P., Arjona A., Zhang Y., Sultana H., Dai J., Yang L., LeBlanc PM., Doiron K., Saleh M., Fikrig E. Apoptosis - Interactions involving proteins with a function related to apoptosis TRIM25 detected via anti-tag western blot maya.saleh@mcgill.ca species of caspase-12 determined via correspondence orchard Only protein-protein interactions Curated by: InnateDB - a knowledge resource for innate immunity mouse-neuron Mus musculus neuronal cell neuron Neuron [Neuronal cell; Neural cell fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Wang P., Arjona A., Zhang Y., Sultana H., Dai J., Yang L., LeBlanc PM., Doiron K., Saleh M., Fikrig E. Apoptosis - Interactions involving proteins with a function related to apoptosis TRIM25 detected via anti-tag western blot RIG-I was detected via immunostaining in primary cortical neurons. maya.saleh@mcgill.ca species of caspase-12 determined via correspondence Accepted 2011-APR-04 by ORCHARD Nat. Immunol. (1529-2908) 2010 orchard Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity ddx58_human Antiviral innate immune response receptor RIG-I DEAD box protein 58 Retinoic acid-inducible gene 1 protein DDX58 Probable ATP-dependent RNA helicase DDX58 Retinoic acid-inducible gene I protein RIG-I-like receptor 1 protein protein human Homo sapiens Human MTTEQRRSLQAFQDYIRKTLDPTYILSYMAPWFREEEVQYIQAEKNNKGPMEAATLFLKFLLELQEEGWFRGFLDALDHAGYSGLYEAIESWDFKKIEKLEEYRLLLKRLQPEFKTRIIPTDIISDLSECLINQECEEILQICSTKGMMAGAEKLVECLLRSDKENWPKTLKLALEKERNKFSELWIVEKGIKDVETEDLEDKMETSDIQIFYQEDPECQNLSENSCPPSEVSDTNLYSPFKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQKGKVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVENNDIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNYLDQKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVIATVKHNLEELEQVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRICKDLENLSQIQNREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYNDALIISEHARMKDALDYLKDFFSNVRAAGFDEIEQDLTQRFEEKLQELESVSRDPSNENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEGNPKLSFLKPGILTGRGKTNQNTGMTLPAQKCILDAFKASGDHNILIATSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTSNAGVIEKEQINMYKEKMMNDSILRLQTWDEAVFREKILHIQTHEKFIRDSQEKPKPVPDKENKKLLCRKCKALACYTADVRVIEECHYTVLGDAFKECFVSRPHPKPKQFSSFEKRAKIFCARQNCSHDWGIHVKYKTFEIPVIKIESFVVEDIATGVQTLYSKWKDFHFEKIPFDPAEMSK BF0D501C395BAE25 tri25_human E3 ubiquitin/ISG15 ligase TRIM25 TRIM25 EFP RNF147 ZNF147 Zinc finger protein 147 Estrogen-responsive finger protein RING finger protein 147 Ubiquitin/ISG15-conjugating enzyme TRIM25 Tripartite motif-containing protein 25 RING-type E3 ubiquitin transferase RING-type E3 ubiquitin transferase TRIM25 protein protein human Homo sapiens Human MAELCPLAEELSCSICLEPFKEPVTTPCGHNFCGSCLNETWAVQGSPYLCPQCRAVYQARPQLHKNTVLCNVVEQFLQADLAREPPADVWTPPARASAPSPNAQVACDHCLKEAAVKTCLVCMASFCQEHLQPHFDSPAFQDHPLQPPVRDLLRRKCSQHNRLREFFCPEHSECICHICLVEHKTCSPASLSQASADLEATLRHKLTVMYSQINGASRALDDVRNRQQDVRMTANRKVEQLQQEYTEMKALLDASETTSTRKIKEEEKRVNSKFDTIYQILLKKKSEIQTLKEEIEQSLTKRDEFEFLEKASKLRGISTKPVYIPEVELNHKLIKGIHQSTIDLKNELKQCIGRLQEPTPSSGDPGEHDPASTHKSTRPVKKVSKEEKKSKKPPPVPALPSKLPTFGAPEQLVDLKQAGLEAAAKATSSHPNSTSLKAKVLETFLAKSRPELLEYYIKVILDYNTAHNKVALSECYTVASVAEMPQNYRPHPQRFTYCSQVLGLHCYKKGIHYWEVELQKNNFCGVGICYGSMNRQGPESRLGRNSASWCVEWFNTKISAWHNNVEKTLPSTKATRVGVLLNCDHGFVIFFAVADKVHLMYKFRVDFTEALYPAFWVFSAGATLSICSPK EB0AB353F0AD4C80 caspc_rat Caspase-12 Casp12 protein protein rat Rattus norvegicus (Rat) Rat MAAKRTHERDPIYKIKGLAKDMLDGVFDDLMDKNVLNGDELLKIGEGASLILSKAENLVESFFEKTEMAGKIFAGHIANSDKQLSLQFPSDDEEDELQKMFTPSSASESRGKVEDEEMEVNVGVAHASHLMLTVPQGIQSTEVQDSLKLCSRDWFCTMKTERAEEIYPVMEKEGRTRLALIICNKKFDYLFDRDDAETDILNMKELLQNLGYSVVIKENLTAQEMETELMKFAGRPEHQSSDSTFLVFMSHGILEGICGVKHRNKKPDVLHDDTIFTIFNNSNCPSLRNKPKILIMQACRGRHTGTIWVSTSKGIATADTDEECVLSHRWNNSITKAHVETDFIAFKSSTPHNISWKVGKSGSLFISKLIDCFKKYCWCYHLEEIFRKVQYSFEVPGELTQMPTIERVSMTRYFYLFPGN DIP protein Q920D5 original sequence version: 79 DIP protein Q920D5 original sequence version: 40 E00809BBC61461AC ddx58-casp12 1 5 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 5A ddx58-casp12-1 3 5 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 5B casp12-ddx58-1 2 5 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig. 5C MEFs were infected for 12h with WNV (MOI=5) casp12-ddx58-2 4 5 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig. 5C MEFs were infected for 12h with WNV (MOI=5)