IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ GPR120 Is an Omega-3 Fatty Acid Receptor Mediating Potent Anti-inflammatory and Insulin-Sensitizing Effects. GPR120 Is an Omega-3 Fatty Acid Receptor Mediating Potent Anti-inflammatory and Insulin-Sensitizing Effects. Cell (0092-8674) 2010 imex curation Oh da Y., Talukdar S., Bae EJ., Imamura T., Morinaga H., Fan W., Li P., Lu WJ., Watkins SM., Olefsky JM. Apoptosis - Interactions involving proteins with a function related to apoptosis jolefsky@ucsd.edu 21-Dec-2010: Contacted by JYOTI. jyoti Only protein-protein interactions mouse-raw264_7 Mus musculus monocyte macrophage cell line RAW264 raw264_7 Mouse monocyte macrophage cellline RAW264.7 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Oh da Y., Talukdar S., Bae EJ., Imamura T., Morinaga H., Fan W., Li P., Lu WJ., Watkins SM., Olefsky JM. Apoptosis - Interactions involving proteins with a function related to apoptosis jolefsky@ucsd.edu Accepted 2010-OCT-14 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Cell (0092-8674) 2010 jyoti Only protein-protein interactions imex curation GPR120 Is an Omega-3 Fatty Acid Receptor Mediating Potent Anti-inflammatory and Insulin-Sensitizing Effects. GPR120 Is an Omega-3 Fatty Acid Receptor Mediating Potent Anti-inflammatory and Insulin-Sensitizing Effects. Cell (0092-8674) 2010 imex curation Oh da Y., Talukdar S., Bae EJ., Imamura T., Morinaga H., Fan W., Li P., Lu WJ., Watkins SM., Olefsky JM. Apoptosis - Interactions involving proteins with a function related to apoptosis jolefsky@ucsd.edu 21-Dec-2010: Contacted by JYOTI. jyoti Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Oh da Y., Talukdar S., Bae EJ., Imamura T., Morinaga H., Fan W., Li P., Lu WJ., Watkins SM., Olefsky JM. Apoptosis - Interactions involving proteins with a function related to apoptosis Details of constructs obtained from the authors. jolefsky@ucsd.edu Accepted 2010-OCT-14 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Cell (0092-8674) 2010 jyoti Only protein-protein interactions imex curation GPR120 Is an Omega-3 Fatty Acid Receptor Mediating Potent Anti-inflammatory and Insulin-Sensitizing Effects. GPR120 Is an Omega-3 Fatty Acid Receptor Mediating Potent Anti-inflammatory and Insulin-Sensitizing Effects. Cell (0092-8674) 2010 imex curation Oh da Y., Talukdar S., Bae EJ., Imamura T., Morinaga H., Fan W., Li P., Lu WJ., Watkins SM., Olefsky JM. Apoptosis - Interactions involving proteins with a function related to apoptosis jolefsky@ucsd.edu 21-Dec-2010: Contacted by JYOTI. jyoti Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Oh da Y., Talukdar S., Bae EJ., Imamura T., Morinaga H., Fan W., Li P., Lu WJ., Watkins SM., Olefsky JM. Apoptosis - Interactions involving proteins with a function related to apoptosis HA-tagged protein labelled by anti-tag immunostaining. Details of constructs obtained from the authors. jolefsky@ucsd.edu Accepted 2010-OCT-14 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Cell (0092-8674) 2010 jyoti Only protein-protein interactions imex curation GPR120 Is an Omega-3 Fatty Acid Receptor Mediating Potent Anti-inflammatory and Insulin-Sensitizing Effects. GPR120 Is an Omega-3 Fatty Acid Receptor Mediating Potent Anti-inflammatory and Insulin-Sensitizing Effects. Cell (0092-8674) 2010 imex curation Oh da Y., Talukdar S., Bae EJ., Imamura T., Morinaga H., Fan W., Li P., Lu WJ., Watkins SM., Olefsky JM. Apoptosis - Interactions involving proteins with a function related to apoptosis jolefsky@ucsd.edu 21-Dec-2010: Contacted by JYOTI. jyoti Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Oh da Y., Talukdar S., Bae EJ., Imamura T., Morinaga H., Fan W., Li P., Lu WJ., Watkins SM., Olefsky JM. Apoptosis - Interactions involving proteins with a function related to apoptosis Details of constructs obtained from the authors. jolefsky@ucsd.edu Accepted 2010-OCT-14 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Cell (0092-8674) 2010 jyoti Only protein-protein interactions imex curation ffar4_mouse Free fatty acid receptor 4 Ffar4 G-protein coupled receptor 120 G-protein coupled receptor GT01 Gpr120 O3far1 Omega-3 fatty acid receptor 1 protein protein mouse Mus musculus Mouse MSPECAQTTGPGPSHTLDQVNRTHFPFFSDVKGDHRLVLSVVETTVLGLIFVVSLLGNVCALVLVARRRRRGATASLVLNLFCADLLFTSAIPLVLVVRWTEAWLLGPVVCHLLFYVMTMSGSVTILTLAAVSLERMVCIVRLRRGLSGPGRRTQAALLAFIWGYSALAALPLCILFRVVPQRLPGGDQEIPICTLDWPNRIGEISWDVFFVTLNFLVPGLVIVISYSKILQITKASRKRLTLSLAYSESHQIRVSQQDYRLFRTLFLLMVSFFIMWSPIIITILLILIQNFRQDLVIWPSLFFWVVAFTFANSALNPILYNMSLFRNEWRKIFCCFFFPEKGAIFTDTSVRRNDLSVISS D3A3F0BEB55F7B7B tab1_mouse TGF-beta-activated kinase 1 and MAP3K7-binding protein 1 Tab1 Map3k7ip1 TGF-beta-activated kinase 1-binding protein 1 Mitogen-activated protein kinase kinase kinase 7-interacting protein 1 protein protein mouse Mus musculus Mouse MAAQRRSLLQSEQQPSWTDDLPLCHLSGVGSASNRSYSADGKGTESHPPEDNWLKFRSENNCFLYGVFNGYDGNRVTNFVAQRLSAELLLGQLNTEHTEADVRRVLLQAFDVVERSFLESIDDALAEKASLQSQLPEGVPQHQLPPQYQKILERLKALEREISGGAMAVVAVLLNSKLYVANVGTNRALLCKSTVDGLQVTQLNMDHTTENEDELFRLSQLGLDAGKIKQMGVICGQESTRRIGDYKVKYGYTDIDLLSAAKSKPIIAEPEIHGAQPLDGVTGFLVLMSEGLYKALEAAHGPGQANQEIAAMIDTEFAKQTSLDAVAQAVVDRVKRIHSDTFASGGERAKFCPRHEDMTLLVRNFGYPLGEMSQPTPTPAPGGRVYPVSVPYSSAQSTSKTSVTLSLVMPSQGQMVNGSHSASTLDEATPTLTNQSPTLTLQSTNTHTQSSSSSSDGGLFRSRPAHSLPPGEDGRVEPYVDFAEFYRLWSVDHGEQSVMTAP F7529D2E3CF30696 arrb2_mouse Beta-arrestin-2 Arrestin beta-2 Arrb2 protein protein mouse Mus musculus Mouse MGEKPGTRVFKKSSPNCKLTVYLGKRDFVDHLDKVDPVDGVVLVDPDYLKDRKVFVTLTCAFRYGREDLDVLGLSFRKDLFIATYQAFPPMPNPPRPPTRLQDRLLKKLGQHAHPFFFTIPQNLPCSVTLQPGPEDTGKACGVDFEIRAFCAKSIEEKSHKRNSVRLIIRKVQFAPETPGPQPSAETTRHFLMSDRRSLHLEASLDKELYYHGEPLNVNVHVTNNSAKTVKKIRVSVRQYADICLFSTAQYKCPVAQLEQDDQVSPSSTFCKVYTITPLLSDNREKRGLALDGQLKHEDTNLASSTIVKEGANKEVLGILVSYRVKVKLVVSRGGDVSVELPFVLMHPKPHDHITLPRPQSAPRETDVPVDTNLIEFDTNYATDDDIVFEDFARLRLKGMKDDDCDDQFC DIP protein Q91YI4 original sequence version: 115 DIP protein Q91YI4 original sequence version: 105 DIP protein Q91YI4 original sequence version: 92 DIP protein Q91YI4 original sequence version: 71 0DFA73A1C532AE03 tab1_human TGF-beta-activated kinase 1 and MAP3K7-binding protein 1 TAB1 MAP3K7IP1 TGF-beta-activated kinase 1-binding protein 1 Mitogen-activated protein kinase kinase kinase 7-interacting protein 1 protein protein human Homo sapiens Human MAAQRRSLLQSEQQPSWTDDLPLCHLSGVGSASNRSYSADGKGTESHPPEDSWLKFRSENNCFLYGVFNGYDGNRVTNFVAQRLSAELLLGQLNAEHAEADVRRVLLQAFDVVERSFLESIDDALAEKASLQSQLPEGVPQHQLPPQYQKILERLKTLEREISGGAMAVVAVLLNNKLYVANVGTNRALLCKSTVDGLQVTQLNVDHTTENEDELFRLSQLGLDAGKIKQVGIICGQESTRRIGDYKVKYGYTDIDLLSAAKSKPIIAEPEIHGAQPLDGVTGFLVLMSEGLYKALEAAHGPGQANQEIAAMIDTEFAKQTSLDAVAQAVVDRVKRIHSDTFASGGERARFCPRHEDMTLLVRNFGYPLGEMSQPTPSPAPAAGGRVYPVSVPYSSAQSTSKTSVTLSLVMPSQGQMVNGAHSASTLDEATPTLTNQSPTLTLQSTNTHTQSSSSSSDGGLFRSRPAHSLPPGEDGRVEPYVDFAEFYRLWSVDHGEQSVVTAP DIP protein Q15750 original sequence version: 74 DIP protein Q15750 original sequence version: 110 DIP protein Q15750 original sequence version: 111 A45743288718983A m3k7_mouse Mitogen-activated protein kinase kinase kinase 7 Map3k7 Tak1 Transforming growth factor-beta-activated kinase 1 protein protein mouse Mus musculus Mouse MSTASAASSSSSSSASEMIEAPSQVLNFEEIDYKEIEVEEVVGRGAFGVVCKAKWRAKDVAIKQIESESERKAFIVELRQLSRVNHPNIVKLYGACLNPVCLVMEYAEGGSLYNVLHGAEPLPYYTAAHAMSWCLQCSQGVAYLHSMQPKALIHRDLKPPNLLLVAGGTVLKICDFGTACDIQTHMTNNKGSAAWMAPEVFEGSNYSEKCDVFSWGIILWEVITRRKPFDEIGGPAFRIMWAVHNGTRPPLIKNLPKPIESLMTRCWSKDPSQRPSMEEIVKIMTHLMRYFPGADEPLQYPCQYSDEGQSNSATSTGSFMDIASTNTSNKSDTNMEQVPATNDTIKRLESKLLKNQAKQQSESGRLSLGASRGSSVESLPPTSEGKRMSADMSEIEARIVATAGNGQPRRRSIQDLTVTGTEPGQVSSRSSSPSVRMITTSGPTSEKPARSHPWTPDDSTDTNGSDNSIPMAYLTLDHQLQPLAPCPNSKESMAVFEQHCKMAQEYMKVQTEIALLLQRKQELVAELDQDEKDQQNTSRLVQEHKKLLDENKSLSTYYQQCKKQLEVIRSQQQKRQGTS DIP protein Q62073 original sequence version: 121 DIP protein Q62073 original sequence version: 108 DIP protein Q62073 original sequence version: 151 97C8F6F3C8E283EE arrb2-gpr120-1 1 7 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association Coimmunoprecipitation between GPR120 and b-arrestin2 with DHA stimulation for 30 min in RAW 264.7 cells Docosahexaenoic acid (DHA) Fig. 3E arrb2-tab1-1 1 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association Docosahexaenoic acid (DHA) - addition of lipopolysaccharide (LPS) partially antagonizes the effect. Fig. 3G tak1-tab1-1 1 6 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association Lipopolysaccharide (LPS) - addition of Docosahexaenoic acid (DHA) partially antagonizes the effect. Fig. 3H arrb2-gpr120-2 3 5 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization GPR120 (red) and b-arrestin2 (green) were localized by confocal microscopy Docosahexaenoic acid (DHA) Fig. 3D arrb2-gpr120-3 4 7 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association DHA-stimulated binding of b-arrestin2 to activated GPR120 was also detected by coimmunoprecipitation Docosahexaenoic acid (DHA) Fig. 3F arrb2-gpr120-4 4 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association only the full-length b-arrestin2 coprecipitated with GPR120 and TAB1, while a series of deletion/truncation b-arrestin2 mutants did not, indicating that the interactions are dependent on the complete tertiary structure of b-arrestin2 Docosahexaenoic acid (DHA) - 30 minutes Supp. Fig. 2D Full-length b-arrestin2 coprecipitated with GPR120, while a series of deletion/truncation b-arrestin2 mutants did not, indicating that the interactions are dependent on the complete tertiary structure of b-arrestin2 arrb2-tab1-2 2 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey physical association physical association only the full-length b-arrestin2 coprecipitated with GPR120 and TAB1, while a series of deletion/truncation b-arrestin2 mutants did not, indicating that the interactions are dependent on the complete tertiary structure of b-arrestin2 Docosahexaenoic acid (DHA) - 60 minutes Supp. Fig. 2D Full-length b-arrestin2 coprecipitated with TAB1, while a series of deletion/truncation b-arrestin2 mutants did not, indicating that the interactions are dependent on the complete tertiary structure of b-arrestin2