IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ DAXX is a new AIRE-interacting protein. DAXX is a new AIRE-interacting protein. J. Biol. Chem. (0021-9258) 2010 imex curation Meloni A., Meloni A., Fiorillo E., Corda D., Incani F., Serra M.L., Contini A., Cao A., Rosatelli M.C. Apoptosis - Interactions involving proteins with a function related to apoptosis Rare diseases - Interactions investigated in the context of Rare genetic disease Origin of AIRE construct inferred from sequence length and mutation mapping. rosatelli@unica.it 23-Sept-2011: Contacted by JYOTI. mgt Only protein-protein interactions chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Meloni A., Meloni A., Fiorillo E., Corda D., Incani F., Serra M.L., Contini A., Cao A., Rosatelli M.C. Apoptosis - Interactions involving proteins with a function related to apoptosis Rare diseases - Interactions investigated in the context of Rare genetic disease Origin of AIRE construct inferred from sequence length and mutation mapping. rosatelli@unica.it Accepted 2011-AUG-23 by MGT 23-Sept-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2010 mgt Only protein-protein interactions imex curation DAXX is a new AIRE-interacting protein. DAXX is a new AIRE-interacting protein. J. Biol. Chem. (0021-9258) 2010 imex curation Meloni A., Meloni A., Fiorillo E., Corda D., Incani F., Serra M.L., Contini A., Cao A., Rosatelli M.C. Apoptosis - Interactions involving proteins with a function related to apoptosis Rare diseases - Interactions investigated in the context of Rare genetic disease Origin of AIRE construct inferred from sequence length and mutation mapping. rosatelli@unica.it 23-Sept-2011: Contacted by JYOTI. mgt Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Meloni A., Meloni A., Fiorillo E., Corda D., Incani F., Serra M.L., Contini A., Cao A., Rosatelli M.C. Apoptosis - Interactions involving proteins with a function related to apoptosis Rare diseases - Interactions investigated in the context of Rare genetic disease Origin of AIRE construct inferred from sequence length and mutation mapping. rosatelli@unica.it Accepted 2011-AUG-23 by MGT 23-Sept-2011: Contacted by JYOTI. A human thymus Matchmaker cDNA library. J. Biol. Chem. (0021-9258) 2010 mgt Only protein-protein interactions imex curation DAXX is a new AIRE-interacting protein. DAXX is a new AIRE-interacting protein. J. Biol. Chem. (0021-9258) 2010 imex curation Meloni A., Meloni A., Fiorillo E., Corda D., Incani F., Serra M.L., Contini A., Cao A., Rosatelli M.C. Apoptosis - Interactions involving proteins with a function related to apoptosis Rare diseases - Interactions investigated in the context of Rare genetic disease Origin of AIRE construct inferred from sequence length and mutation mapping. rosatelli@unica.it 23-Sept-2011: Contacted by JYOTI. mgt Only protein-protein interactions chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Meloni A., Meloni A., Fiorillo E., Corda D., Incani F., Serra M.L., Contini A., Cao A., Rosatelli M.C. Apoptosis - Interactions involving proteins with a function related to apoptosis Rare diseases - Interactions investigated in the context of Rare genetic disease Origin of AIRE construct inferred from sequence length and mutation mapping. rosatelli@unica.it Accepted 2011-AUG-23 by MGT 23-Sept-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2010 mgt Only protein-protein interactions imex curation DAXX is a new AIRE-interacting protein. DAXX is a new AIRE-interacting protein. J. Biol. Chem. (0021-9258) 2010 imex curation Meloni A., Meloni A., Fiorillo E., Corda D., Incani F., Serra M.L., Contini A., Cao A., Rosatelli M.C. Apoptosis - Interactions involving proteins with a function related to apoptosis Rare diseases - Interactions investigated in the context of Rare genetic disease Origin of AIRE construct inferred from sequence length and mutation mapping. rosatelli@unica.it 23-Sept-2011: Contacted by JYOTI. mgt Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Meloni A., Meloni A., Fiorillo E., Corda D., Incani F., Serra M.L., Contini A., Cao A., Rosatelli M.C. Apoptosis - Interactions involving proteins with a function related to apoptosis Rare diseases - Interactions investigated in the context of Rare genetic disease Untagged proteins recognised by anti-bait Ab, and a fluorescently labelled secondary antibody. Origin of AIRE construct inferred from sequence length and mutation mapping. rosatelli@unica.it Accepted 2011-AUG-23 by MGT 23-Sept-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2010 mgt Only protein-protein interactions imex curation DAXX is a new AIRE-interacting protein. DAXX is a new AIRE-interacting protein. J. Biol. Chem. (0021-9258) 2010 imex curation Meloni A., Meloni A., Fiorillo E., Corda D., Incani F., Serra M.L., Contini A., Cao A., Rosatelli M.C. Apoptosis - Interactions involving proteins with a function related to apoptosis Rare diseases - Interactions investigated in the context of Rare genetic disease Origin of AIRE construct inferred from sequence length and mutation mapping. rosatelli@unica.it 23-Sept-2011: Contacted by JYOTI. mgt Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Meloni A., Meloni A., Fiorillo E., Corda D., Incani F., Serra M.L., Contini A., Cao A., Rosatelli M.C. Apoptosis - Interactions involving proteins with a function related to apoptosis Rare diseases - Interactions investigated in the context of Rare genetic disease Origin of AIRE construct inferred from sequence length and mutation mapping. rosatelli@unica.it Accepted 2011-AUG-23 by MGT 23-Sept-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2010 mgt Only protein-protein interactions imex curation DAXX is a new AIRE-interacting protein. DAXX is a new AIRE-interacting protein. J. Biol. Chem. (0021-9258) 2010 imex curation Meloni A., Meloni A., Fiorillo E., Corda D., Incani F., Serra M.L., Contini A., Cao A., Rosatelli M.C. Apoptosis - Interactions involving proteins with a function related to apoptosis Rare diseases - Interactions investigated in the context of Rare genetic disease Origin of AIRE construct inferred from sequence length and mutation mapping. rosatelli@unica.it 23-Sept-2011: Contacted by JYOTI. mgt Only protein-protein interactions chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Meloni A., Meloni A., Fiorillo E., Corda D., Incani F., Serra M.L., Contini A., Cao A., Rosatelli M.C. Apoptosis - Interactions involving proteins with a function related to apoptosis Rare diseases - Interactions investigated in the context of Rare genetic disease Origin of AIRE construct inferred from sequence length and mutation mapping. rosatelli@unica.it Accepted 2011-AUG-23 by MGT 23-Sept-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2010 mgt Only protein-protein interactions imex curation aire_human Autoimmune regulator AIRE APECED Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein protein protein human Homo sapiens Human MATDAALRRLLRLHRTEIAVAVDSAFPLLHALADHDVVPEDKFQETLHLKEKEGCPQAFHALLSWLLTQDSTAILDFWRVLFKDYNLERYGRLQPILDSFPKDVDLSQPRKGRKPPAVPKALVPPPRLPTKRKASEEARAAAPAALTPRGTASPGSQLKAKPPKKPESSAEQQRLPLGNGIQTMSASVQRAVAMSSGDVPGARGAVEGILIQQVFESGGSKKCIQVGGEFYTPSKFEDSGSGKNKARSSSGPKPLVRAKGAQGAAPGGGEARLGQQGSVPAPLALPSDPQLHQKNEDECAVCRDGGELICCDGCPRAFHLACLSPPLREIPSGTWRCSSCLQATVQEVQPRAEEPRPQEPPVETPLPPGLRSAGEEVRGPPGEPLAGMDTTLVYKHLPAPPSAAPLPGLDSSALHPLLCVGPEGQQNLAPGARCGVCGDGTDVLRCTHCAAAFHWRCHFPAGTSRPGTGLRCRSCSGDVTPAPVEGVLAPSPARLAPGPAKDDTASHEPALHRDDLESLLSEHTFDGILQWAIQSMARPAAPFPS 8CF703F8C9411BC5 daxx_human Death domain-associated protein 6 DAXX BING2 DAP6 Daxx Fas death domain-associated protein ETS1-associated protein 1 protein protein human Homo sapiens Human MATANSIIVLDDDDEDEAAAQPGPSHPLPNAASPGAEAPSSSEPHGARGSSSSGGKKCYKLENEKLFEEFLELCKMQTADHPEVVPFLYNRQQRAHSLFLASAEFCNILSRVLSRARSRPAKLYVYINELCTVLKAHSAKKKLNLAPAATTSNEPSGNNPPTHLSLDPTNAENTASQSPRTRGSRRQIQRLEQLLALYVAEIRRLQEKELDLSELDDPDSAYLQEARLKRKLIRLFGRLCELKDCSSLTGRVIEQRIPYRGTRYPEVNRRIERLINKPGPDTFPDYGDVLRAVEKAAARHSLGLPRQQLQLMAQDAFRDVGIRLQERRHLDLIYNFGCHLTDDYRPGVDPALSDPVLARRLRENRSLAMSRLDEVISKYAMLQDKSEEGERKKRRARLQGTSSHSADTPEASLDSGEGPSGMASQGCPSASRAETDDEDDEESDEEEEEEEEEEEEEATDSEEEEDLEQMQEGQEDDEEEDEEEEAAAGKDGDKSPMSSLQISNEKNLEPGKQISRSSGEQQNKGRIVSPSLLSEEPLAPSSIDAESNGEQPEELTLEEESPVSQLFELEIEALPLDTPSSVETDISSSRKQSEEPFTTVLENGAGMVSSTSFNGGVSPHNWGDSGPPCKKSRKEKKQTGSGPLGNSYVERQRSVHEKNGKKICTLPSPPSPLASLAPVADSSTRVDSPSHGLVTSSLCIPSPARLSQTPHSQPPRPGTCKTSVATQCDPEEIIVLSDSD 1B309ADDAA878040 daxx_cerae_protein Death domain-associated protein 6 protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey Similar to O35613 hdac1_cerae_protein Histone deacetylase 1 protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey Similar to O09106 hdac2_cerae_protein Histone deacetylase 2 protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey aire-daxx 5 8 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain O43918:p.Trp78Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W R Wed May 02 19:02:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp78arg' Tue Jun 30 17:12:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp78Arg' physical association physical association Fig. 1 aire-daxx-1 2 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 21 19 physical association physical association aire-daxx-2 5 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 1 aire-daxx-3 1 8 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig 1 aire-daxx-4 1 7 unspecified role unspecified role bait bait region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey physical association physical association Fig 2C aire-daxx-5 4 8 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig. 4 aire-aire-6 3 7 unspecified role unspecified role bait bait region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig 6 hdac1-aire 6 7 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 10 unspecified role unspecified role bait bait physical association physical association Fig. 8 hdac2-aire 6 7 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role bait bait physical association physical association Fig. 8