UniProt UniProt http://www.uniprot.org DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1. DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1. J. Biol. Chem. (0021-9258) 2010 mimix curation Guo X., Williams J.G., Schug T.T., Li X. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of p53 (deacetylase assay fig. 3d) according pmid 11672522 (seq numbering) lix3@niehs.nih.gov 21-Dec-2011: Contacted by JYOTI. murine testis cDNA library in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Guo X., Williams J.G., Schug T.T., Li X. Apoptosis - Interactions involving proteins with a function related to apoptosis though in vitrro translated HA-tagged DYRKs have been used, for detection anti-dyrk antibodies have beeen used. no hint for ant-HA ab. Origin of p53 (deacetylase assay fig. 3d) according pmid 11672522 (seq numbering) lix3@niehs.nih.gov Accepted 2011-NOV-15 by MGT 21-Dec-2011: Contacted by JYOTI. murine testis cDNA library J. Biol. Chem. (0021-9258) 2010 mimix curation DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1. DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1. J. Biol. Chem. (0021-9258) 2010 mimix curation Guo X., Williams J.G., Schug T.T., Li X. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of p53 (deacetylase assay fig. 3d) according pmid 11672522 (seq numbering) lix3@niehs.nih.gov 21-Dec-2011: Contacted by JYOTI. murine testis cDNA library in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Guo X., Williams J.G., Schug T.T., Li X. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of p53 (deacetylase assay fig. 3d) according pmid 11672522 (seq numbering) lix3@niehs.nih.gov Accepted 2011-NOV-15 by MGT 21-Dec-2011: Contacted by JYOTI. murine testis cDNA library J. Biol. Chem. (0021-9258) 2010 mimix curation DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1. DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1. J. Biol. Chem. (0021-9258) 2010 mimix curation Guo X., Williams J.G., Schug T.T., Li X. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of p53 (deacetylase assay fig. 3d) according pmid 11672522 (seq numbering) lix3@niehs.nih.gov 21-Dec-2011: Contacted by JYOTI. murine testis cDNA library human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Guo X., Williams J.G., Schug T.T., Li X. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of p53 (deacetylase assay fig. 3d) according pmid 11672522 (seq numbering) lix3@niehs.nih.gov Accepted 2011-NOV-15 by MGT 21-Dec-2011: Contacted by JYOTI. murine testis cDNA library J. Biol. Chem. (0021-9258) 2010 mimix curation DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1. DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1. J. Biol. Chem. (0021-9258) 2010 mimix curation Guo X., Williams J.G., Schug T.T., Li X. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of p53 (deacetylase assay fig. 3d) according pmid 11672522 (seq numbering) lix3@niehs.nih.gov 21-Dec-2011: Contacted by JYOTI. murine testis cDNA library yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Guo X., Williams J.G., Schug T.T., Li X. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of p53 (deacetylase assay fig. 3d) according pmid 11672522 (seq numbering) lix3@niehs.nih.gov Accepted 2011-NOV-15 by MGT murine testis cDNA library J. Biol. Chem. (0021-9258) 2010 mimix curation DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1. DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1. J. Biol. Chem. (0021-9258) 2010 mimix curation Guo X., Williams J.G., Schug T.T., Li X. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of p53 (deacetylase assay fig. 3d) according pmid 11672522 (seq numbering) lix3@niehs.nih.gov 21-Dec-2011: Contacted by JYOTI. murine testis cDNA library in vitro In vitro deacetylase assay deacetylase assay predetermined predetermined participant predetermined Guo X., Williams J.G., Schug T.T., Li X. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of p53 (deacetylase assay fig. 3d) according pmid 11672522 (seq numbering) lix3@niehs.nih.gov Accepted 2011-NOV-15 by MGT 21-Dec-2011: Contacted by JYOTI. murine testis cDNA library J. Biol. Chem. (0021-9258) 2010 mimix curation DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1. DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1. J. Biol. Chem. (0021-9258) 2010 mimix curation Guo X., Williams J.G., Schug T.T., Li X. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of p53 (deacetylase assay fig. 3d) according pmid 11672522 (seq numbering) lix3@niehs.nih.gov 21-Dec-2011: Contacted by JYOTI. murine testis cDNA library mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Guo X., Williams J.G., Schug T.T., Li X. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of p53 (deacetylase assay fig. 3d) according pmid 11672522 (seq numbering) lix3@niehs.nih.gov Accepted 2011-NOV-15 by MGT 21-Dec-2011: Contacted by JYOTI. murine testis cDNA library J. Biol. Chem. (0021-9258) 2010 mimix curation DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1. DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1. J. Biol. Chem. (0021-9258) 2010 mimix curation Guo X., Williams J.G., Schug T.T., Li X. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of p53 (deacetylase assay fig. 3d) according pmid 11672522 (seq numbering) lix3@niehs.nih.gov 21-Dec-2011: Contacted by JYOTI. murine testis cDNA library yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Guo X., Williams J.G., Schug T.T., Li X. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of p53 (deacetylase assay fig. 3d) according pmid 11672522 (seq numbering) lix3@niehs.nih.gov Accepted 2011-NOV-15 by MGT 21-Dec-2011: Contacted by JYOTI. murine testis cDNA library J. Biol. Chem. (0021-9258) 2010 mimix curation DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1. DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1. J. Biol. Chem. (0021-9258) 2010 mimix curation Guo X., Williams J.G., Schug T.T., Li X. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of p53 (deacetylase assay fig. 3d) according pmid 11672522 (seq numbering) lix3@niehs.nih.gov 21-Dec-2011: Contacted by JYOTI. murine testis cDNA library human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Guo X., Williams J.G., Schug T.T., Li X. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of p53 (deacetylase assay fig. 3d) according pmid 11672522 (seq numbering) lix3@niehs.nih.gov Accepted 2011-NOV-15 by MGT 21-Dec-2011: Contacted by JYOTI. murine testis cDNA library J. Biol. Chem. (0021-9258) 2010 mimix curation dyr1a_mouse Dual specificity tyrosine-phosphorylation-regulated kinase 1A Dyrk1a Dyrk Protein kinase minibrain homolog MP86 Dual specificity YAK1-related kinase protein protein mouse Mus musculus Mouse MHTGGETSACKPSSVRLAPSFSFHAAGLQMAAQMPHSHQYSDRRQPSISDQQVSALPYSDQIQQPLTNQVMPDIVMLQRRMPQTFRDPATAPLRKLSVDLIKTYKHINEVYYAKKKRRHQQGQGDDSSHKKERKVYNDGYDDDNYDYIVKNGEKWMDRYEIDSLIGKGSFGQVVKAYDRVEQEWVAIKIIKNKKAFLNQAQIEVRLLELMNKHDTEMKYYIVHLKRHFMFRNHLCLVFEMLSYNLYDLLRNTNFRGVSLNLTRKFAQQMCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYRSPEVLLGMPYDLAIDMWSLGCILVEMHTGEPLFSGANEVDQMNKIVEVLGIPPAHILDQAPKARKFFEKLPDGTWSLKKTKDGKREYKPPGTRKLHNILGVETGGPGGRRAGESGHTVADYLKFKDLILRMLDYDPKTRIQPYYALQHSFFKKTADEGTNTSNSVSTSPAMEQSQSSGTTSSTSSSSGGSSGTSNSGRARSDPTHQHRHSGGHFAAAVQAMDCETHSPQVRQQFPAPLGWSGTEAPTQVTVETHPVQETTFHVAPQQNALHHHHGNSSHHHHHHHHHHHHHGQQALGNRTRPRVYNSPTNSSSTQDSMEVGHSHHSMTSLSSSTTSSSTSSSSTGNQGNQAYQNRPVAANTLDFGQNGAMDVNLTVYSNPRQETGIAGHPTYQFSANTGPAHYMTEGHLAMRQGADREESPMTGVCVQQSPVASS E117DDD6C5E8C74F p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD DIP protein P04637 original sequence version: 121 DIP protein P04637 original sequence version: 115 DIP protein P04637-1 original sequence version: 224 DIP protein P04637 original sequence version: 117 DIP protein P04637-1 original sequence version: 226 DIP protein P04637 original sequence version: 226 DIP protein P04637 original sequence version: 212 DIP protein P04637 original sequence version: 220 DIP protein P04637-1 original sequence version: 220 DIP protein P04637-1 original sequence version: 145 DIP protein P04637 original sequence version: 146 DIP protein P04637 original sequence version: 145 DIP protein P04637 original sequence version: 166 DIP protein P04637-1 original sequence version: 166 DIP protein P04637 original sequence version: 169 DIP protein P04637 original sequence version: 177 DIP protein P04637 original sequence version: 175 DIP protein P04637-1 original sequence version: 175 DIP protein P04637-1 original sequence version: 172 DIP protein P04637-1 original sequence version: 169 DIP protein P04637 original sequence version: 170 DIP protein P04637 original sequence version: 172 DIP protein P04637-1 original sequence version: 194 DIP protein P04637 original sequence version: 196 DIP protein P04637 original sequence version: 185 DIP protein P04637-1 original sequence version: 191 DIP protein P04637 original sequence version: 191 DIP protein P04637 original sequence version: 180 DIP protein P04637-1 original sequence version: 197 DIP protein P04637 original sequence version: 183 DIP protein P04637-1 original sequence version: 183 DIP protein P04637-1 original sequence version: 185 DIP protein P04637 original sequence version: 199 DIP protein P04637 original sequence version: 201 DIP protein P04637 original sequence version: 189 DIP protein P04637 original sequence version: 235 DIP protein P04637 original sequence version: 230 DIP protein P04637 original sequence version: 234 DIP protein P04637-1 original sequence version: 234 DIP protein P04637-1 original sequence version: 235 DIP protein P04637 original sequence version: 239 DIP protein P04637-1 original sequence version: 212 DIP protein P04637-1 original sequence version: 213 DIP protein P04637 original sequence version: 213 DIP protein P04637 original sequence version: 206 DIP protein P04637-1 original sequence version: 188 DIP protein P04637-1 original sequence version: 228 DIP protein P04637 original sequence version: 208 DIP protein P04637 original sequence version: 240 DIP protein P04637-1 original sequence version: 240 DIP protein P04637 original sequence version: 204 DIP protein P04637-1 original sequence version: 204 DIP protein P04637-1 original sequence version: 209 DIP protein P04637-1 original sequence version: 203 DIP protein P04637 original sequence version: 221 DIP protein P04637-1 original sequence version: 152 DIP protein P04637 original sequence version: 217 DIP protein P04637-1 original sequence version: 140 DIP protein P04637 original sequence version: 215 DIP protein P04637 original sequence version: 214 DIP protein P04637-1 original sequence version: 182 DIP protein P04637 original sequence version: 182 DIP protein P04637 original sequence version: 188 DIP protein P04637 original sequence version: 192 DIP protein P04637-1 original sequence version: 192 DIP protein P04637 original sequence version: 218 DIP protein P04637-1 original sequence version: 218 AD5C149FD8106131 dyr1a_human Dual specificity tyrosine-phosphorylation-regulated kinase 1A Protein kinase minibrain homolog HP86 Dual specificity YAK1-related kinase DYRK1A DYRK MNB MNBH protein protein human Homo sapiens Human MHTGGETSACKPSSVRLAPSFSFHAAGLQMAGQMPHSHQYSDRRQPNISDQQVSALSYSDQIQQPLTNQVMPDIVMLQRRMPQTFRDPATAPLRKLSVDLIKTYKHINEVYYAKKKRRHQQGQGDDSSHKKERKVYNDGYDDDNYDYIVKNGEKWMDRYEIDSLIGKGSFGQVVKAYDRVEQEWVAIKIIKNKKAFLNQAQIEVRLLELMNKHDTEMKYYIVHLKRHFMFRNHLCLVFEMLSYNLYDLLRNTNFRGVSLNLTRKFAQQMCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYRSPEVLLGMPYDLAIDMWSLGCILVEMHTGEPLFSGANEVDQMNKIVEVLGIPPAHILDQAPKARKFFEKLPDGTWNLKKTKDGKREYKPPGTRKLHNILGVETGGPGGRRAGESGHTVADYLKFKDLILRMLDYDPKTRIQPYYALQHSFFKKTADEGTNTSNSVSTSPAMEQSQSSGTTSSTSSSSGGSSGTSNSGRARSDPTHQHRHSGGHFTAAVQAMDCETHSPQVRQQFPAPLGWSGTEAPTQVTVETHPVQETTFHVAPQQNALHHHHGNSSHHHHHHHHHHHHHGQQALGNRTRPRVYNSPTNSSSTQDSMEVGHSHHSMTSLSSSTTSSSTSSSSTGNQGNQAYQNRPVAANTLDFGQNGAMDVNLTVYSNPRQETGIAGHPTYQFSANTGPAHYMTEGHLTMRQGADREESPMTGVCVQQSPVASS 7C3A52A3CBB04FB5 sir1_human NAD-dependent protein deacetylase sirtuin-1 NAD-dependent protein deacylase sirtuin-1 SIRT1 SIR2L1 SIR2-like protein 1 Regulatory protein SIR2 homolog 1 protein protein human Homo sapiens Human MADEAALALQPGGSPSAAGADREAASSPAGEPLRKRPRRDGPGLERSPGEPGGAAPEREVPAAARGCPGAAAAALWREAEAEAAAAGGEQEAQATAAAGEGDNGPGLQGPSREPPLADNLYDEDDDDEGEEEEEAAAAAIGYRDNLLFGDEIITNGFHSCESDEEDRASHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQRIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRTQKELAYLSELPPTPLHVSEDSSSPERTSPPDSSVIVTLLDQAAKSNDDLDVSESKGCMEEKPQEVQTSRNVESIAEQMENPDLKNVGSSTGEKNERTSVAGTVRKCWPNRVAKEQISRRLDGNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESEIEEFYNGLEDEPDVPERAGGAGFGTDGDDQEAINEAISVKQEVTDMNYPSNKS DIP protein Q96EB6 original sequence version: 140 DIP protein Q96EB6 original sequence version: 71 DIP protein Q96EB6 original sequence version: 125 DIP protein Q96EB6 original sequence version: 115 DIP protein Q96EB6 original sequence version: 63 DIP protein Q96EB6-1 original sequence version: 102 DIP protein Q96EB6 original sequence version: 102 DIP protein Q96EB6 original sequence version: 98 DIP protein Q96EB6 original sequence version: 144 DIP protein Q96EB6 original sequence version: 120 DIP protein Q96EB6-1 original sequence version: 120 DIP protein Q96EB6 original sequence version: 113 DIP protein Q96EB6-1 original sequence version: 117 2D3BEA6D73DA229F dyrk3_mouse Dual specificity tyrosine-phosphorylation-regulated kinase 3 Dyrk3 protein protein mouse Mus musculus Mouse MGGAARDRGRKDAALPGAGLPPQQRRLGDGVYDTFMMIDETKGPPYSDTFSNPSEAPVSRRLNITTEPLTRGHTQHFVNGSEMKVEQLFQEFGNRRSNTLQSDGISNSEKSSPASQGKSSESLSAVKCNLSSRPSKVLPLTPEQALKQYKHHLTAYEKLEIVSYPEIYFVGPNAKKRQGVIGGPNNGGYDDADGAYIHVPRDHLAYRYEVLKIIGKGSFGQVARVYDHKLRQYVALKMVRNEKRFHRQAAEEIRILEHLKKQDKTGSMNVIHMLESFTFRNHVCMAFELLSIDLYELIKKNKFQGFSVQLVRKFAQSILQSLDALHKNKIIHCDLKPENILLKHHGRSATKVIDFGSSCFEYQKLYTYIQSRFYRAPEIILGCRYSTPIDIWSFGCILAELLTGQPLFPGEDEGDQLACMIELLGMPPQKLLEQSKRAKYFINSKGLPRYCSVSTQTDGRVVLLGGRSRRGKKRGPPGSKDWATALKGCGDYLFIEFLKRCLQWDPSARLTPAQALRHPWISKSTPKPLTMDKVPGKRVVNPTNAFQGLGSKLPPVVGIASKLKANLMSETSGSIPLCSVLPKLIS 0097F7584726AB55 dyrk3_human Dual specificity tyrosine-phosphorylation-regulated kinase 3 Regulatory erythroid kinase DYRK3 protein protein human Homo sapiens Human MGGTARGPGRKDAGPPGAGLPPQQRRLGDGVYDTFMMIDETKCPPCSNVLCNPSEPPPPRRLNMTTEQFTGDHTQHFLDGGEMKVEQLFQEFGNRKSNTIQSDGISDSEKCSPTVSQGKSSDCLNTVKSNSSSKAPKVVPLTPEQALKQYKHHLTAYEKLEIINYPEIYFVGPNAKKRHGVIGGPNNGGYDDADGAYIHVPRDHLAYRYEVLKIIGKGSFGQVARVYDHKLRQYVALKMVRNEKRFHRQAAEEIRILEHLKKQDKTGSMNVIHMLESFTFRNHVCMAFELLSIDLYELIKKNKFQGFSVQLVRKFAQSILQSLDALHKNKIIHCDLKPENILLKHHGRSSTKVIDFGSSCFEYQKLYTYIQSRFYRAPEIILGSRYSTPIDIWSFGCILAELLTGQPLFPGEDEGDQLACMMELLGMPPPKLLEQSKRAKYFINSKGIPRYCSVTTQADGRVVLVGGRSRRGKKRGPPGSKDWGTALKGCDDYLFIEFLKRCLHWDPSARLTPAQALRHPWISKSVPRPLTTIDKVSGKRVVNPASAFQGLGSKLPPVVGIANKLKANLMSETNGSIPLCSVLPKLIS 9950F51C39AFED82 sir1_mouse NAD-dependent protein deacetylase sirtuin-1 NAD-dependent protein deacylase sirtuin-1 Sirt1 Sir2l1 SIR2alpha SIR2-like protein 1 Regulatory protein SIR2 homolog 1 protein protein mouse Mus musculus Mouse MADEVALALQAAGSPSAAAAMEAASQPADEPLRKRPRRDGPGLGRSPGEPSAAVAPAAAGCEAASAAAPAALWREAAGAAASAEREAPATAVAGDGDNGSGLRREPRAADDFDDDEGEEEDEAAAAAAAAAIGYRDNLLLTDGLLTNGFHSCESDDDDRTSHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQRILQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRPQKELVHLSELPPTPLHISEDSSSPERTVPQDSSVIATLVDQATNNNVNDLEVSESSCVEEKPQEVQTSRNVENINVENPDFKAVGSSTADKNERTSVAETVRKCWPNRLAKEQISKRLEGNQYLFVPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPLEDESEIEEFYNGLEDDTERPECAGGSGFGADGGDQEVVNEAIATRQELTDVNYPSDKS DIP protein Q923E4 original sequence version: 83 DIP protein Q923E4 original sequence version: 135 7F15625E29433119 sirt1-dyrk3 4 13 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait physical association physical association no figure sirt1-dyrk3-1 7 13 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait physical association physical association S1A sirt1-dyrk3-2 1 13 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 15 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1A, 1D, 1F, S6A and S1C sirt1-dyrk1a 1 15 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association 1A, S6A and S1C sirt1-dyrk1a-2 6 9 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait association association 1B dyrk3-sirt1 1 13 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey physical association physical association 1E and S1D dyrk1a-dyrk3 8 12 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association S1B dyrk3-sirt1-4 8 14 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey physical association physical association S1B dyrk3-sirt1-3 2 15 enzyme target enzyme target substrate substrate neutral component neutral component 13 enzyme enzyme neutral component neutral component direct interaction direct interaction 3E dyrk1a-dyrk3-1 2 9 enzyme enzyme neutral component neutral component 15 enzyme target enzyme target substrate substrate neutral component neutral component direct interaction direct interaction 3E sirt1-tp53-8 5 10 enzyme target enzyme target substrate substrate neutral component neutral component 15 enzyme enzyme neutral component neutral component direct interaction direct interaction 3D dyrk1a-sirt1 3 9 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component colocalization colocalization 1C dyrk1a-dyrk3-2 3 12 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component colocalization colocalization 1C dyrk1a-sirt1-1 3 9 unspecified role unspecified role neutral component neutral component 15 unspecified role unspecified role neutral component neutral component colocalization colocalization S6B dyrk1a-dyrk3-3 3 15 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component colocalization colocalization S6B