IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Multivalent DR5 peptides activate the TRAIL death pathway and exert tumoricidal activity. Multivalent DR5 peptides activate the TRAIL death pathway and exert tumoricidal activity. Cancer Res. (0008-5472) 2010 imex curation Pavet V., Beyrath J., Pardin C., Morizot A., Lechner M.C., Briand J.P., Wendland M., Maison W., Fournel S., Micheau O., Guichard G., Gronemeyer H. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin and features of TNFSF10, TNFR1 and TNF-alpha were provided by the author upon email request. hg@igbmc.fr 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Pavet V., Beyrath J., Pardin C., Morizot A., Lechner M.C., Briand J.P., Wendland M., Maison W., Fournel S., Micheau O., Guichard G., Gronemeyer H. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin and features of TNFSF10, TNFR1 and TNF-alpha were provided by the author upon email request. hg@igbmc.fr Accepted 2011-DEC-01 by ORCHARD Cancer Res. (0008-5472) 2010 Only protein-protein interactions imex curation Multivalent DR5 peptides activate the TRAIL death pathway and exert tumoricidal activity. Multivalent DR5 peptides activate the TRAIL death pathway and exert tumoricidal activity. Cancer Res. (0008-5472) 2010 imex curation Pavet V., Beyrath J., Pardin C., Morizot A., Lechner M.C., Briand J.P., Wendland M., Maison W., Fournel S., Micheau O., Guichard G., Gronemeyer H. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin and features of TNFSF10, TNFR1 and TNF-alpha were provided by the author upon email request. hg@igbmc.fr 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions human-bjab Homo sapiens B-cell lymphoma cell line bjab B-cell lymphoma cell line, derived from the tumour of an exceptional African case of Burkitt's lymphoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Pavet V., Beyrath J., Pardin C., Morizot A., Lechner M.C., Briand J.P., Wendland M., Maison W., Fournel S., Micheau O., Guichard G., Gronemeyer H. Apoptosis - Interactions involving proteins with a function related to apoptosis The BJAB cell line used in this experiment stably expresses TNFRSF10B (DR5). Origin and features of TNFSF10, TNFR1 and TNF-alpha were provided by the author upon email request. hg@igbmc.fr Accepted 2011-DEC-01 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Cancer Res. (0008-5472) 2010 Only protein-protein interactions imex curation Multivalent DR5 peptides activate the TRAIL death pathway and exert tumoricidal activity. Multivalent DR5 peptides activate the TRAIL death pathway and exert tumoricidal activity. Cancer Res. (0008-5472) 2010 imex curation Pavet V., Beyrath J., Pardin C., Morizot A., Lechner M.C., Briand J.P., Wendland M., Maison W., Fournel S., Micheau O., Guichard G., Gronemeyer H. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin and features of TNFSF10, TNFR1 and TNF-alpha were provided by the author upon email request. hg@igbmc.fr 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions human-bjab Homo sapiens B-cell lymphoma cell line bjab B-cell lymphoma cell line, derived from the tumour of an exceptional African case of Burkitt's lymphoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Pavet V., Beyrath J., Pardin C., Morizot A., Lechner M.C., Briand J.P., Wendland M., Maison W., Fournel S., Micheau O., Guichard G., Gronemeyer H. Apoptosis - Interactions involving proteins with a function related to apoptosis The BJAB cell line used in this experiment stably does not express TNFRSF10B (DR5). Origin and features of TNFSF10, TNFR1 and TNF-alpha were provided by the author upon email request. hg@igbmc.fr Accepted 2011-DEC-01 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Cancer Res. (0008-5472) 2010 Only protein-protein interactions imex curation trailmim/dr5 1t TRAIL mimicking peptide 1-trimer peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro WDCLDNRIGRRQCVKL 3xtrailmim/dr5 1m linked through an adamantyl core. fadd_human FAS-associated death domain protein FAS-associating death domain-containing protein Mediator of receptor induced toxicity FADD MORT1 GIG3 Growth-inhibiting gene 3 protein protein protein human Homo sapiens Human MDPFLVLLHSVSSSLSSSELTELKFLCLGRVGKRKLERVQSGLDLFSMLLEQNDLEPGHTELLRELLASLRRHDLLRRVDDFEAGAAAGAAPGEEDLCAAFNVICDNVGKDWRRLARQLKVSDTKIDSIEDRYPRNLTERVRESLRIWKNTEKENATVAHLVGALRSCQMNLVADLVQEVQQARDLQNRSGAMSPMSWNSDASTSEAS DIP protein Q13158 original sequence version: 155 DIP protein Q13158 original sequence version: 130 DIP protein Q13158 original sequence version: 110 DIP protein Q13158 original sequence version: 118 DIP protein Q13158 original sequence version: 141 DIP protein Q13158 original sequence version: 166 0E65E2F852E83507 tr10c_human Tumor necrosis factor receptor superfamily member 10C Decoy receptor 1 Decoy TRAIL receptor without death domain TNF-related apoptosis-inducing ligand receptor 3 TRAIL receptor without an intracellular domain Lymphocyte inhibitor of TRAIL Antagonist decoy receptor for TRAIL/Apo-2L DCR1 LIT TRAILR3 TRID UNQ321/PRO366 TNFRSF10C protein protein human Homo sapiens Human MARIPKTLKFVVVIVAVLLPVLAYSATTARQEEVPQQTVAPQQQRHSFKGEECPAGSHRSEHTGACNPCTEGVDYTNASNNEPSCFPCTVCKSDQKHKSSCTMTRDTVCQCKEGTFRNENSPEMCRKCSRCPSGEVQVSNCTSWDDIQCVEEFGANATVETPAAEETMNTSPGTPAPAAEETMNTSPGTPAPAAEETMTTSPGTPAPAAEETMTTSPGTPAPAAEETMITSPGTPASSHYLSCTIVGIIVLIVLLIVFV 40CBF7FCEEAE4C69 trailmim/dr5 2d TRAIL mimicking peptide 2-dimer peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro WDCLDNRIGKRQCVRL 2xtrailmim/dr5 2m linked through K10 using bis-succunimidyl carboxymehtoxyacetate. trailmim/dr5 1m TRAIL mimicking peptide 1-monomer peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro WDCLDNRIGRRQCVKL tnf10_human Tumor necrosis factor ligand superfamily member 10 TNF-related apoptosis-inducing ligand Apo-2 ligand TNFSF10 APO2L TRAIL protein protein human Homo sapiens Human MAMMEVQGGPSLGQTCVLIVIFTVLLQSLCVAVTYVYFTNELKQMQDKYSKSGIACFLKEDDSYWDPNDEESMNSPCWQVKWQLRQLVRKMILRTSEETISTVQEKQQNISPLVRERGPQRVAAHITGTRGRSNTLSSPNSKNEKALGRKINSWESSRSGHSFLSNLHLRNGELVIHEKGFYYIYSQTYFRFQEEIKENTKNDKQMVQYIYKYTSYPDPILLMKSARNSCWSKDAEYGLYSIYQGGIFELKENDRIFVSVTNEHLIDMDHEASFFGAFLVG DIP protein P50591 original sequence version: 150 DDAAAF78DAAB2F6D trailmim/dr5 3t TRAIL mimicking peptide 3-trimer peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro WDCLDNKIGRRQCVRL 3xtrailmim/dr5 3m linked through an adamantyl core. trailmim/dr5 3m TRAIL mimicking peptide 3-monomer peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro WDCLDNKIGRRQCVRL casp8_human Caspase-8 CASP8 MCH5 ICE-like apoptotic protease 5 MORT1-associated ced-3 homolog FADD-homologous ICE/ced-3-like protease FADD-like ICE Apoptotic cysteine protease Apoptotic protease Mch-5 CAP4 protein protein human Homo sapiens Human MDFSRNLYDIGEQLDSEDLASLKFLSLDYIPQRKQEPIKDALMLFQRLQEKRMLEESNLSFLKELLFRINRLDLLITYLNTRKEEMERELQTPGRAQISAYRVMLYQISEEVSRSELRSFKFLLQEEISKCKLDDDMNLLDIFIEMEKRVILGEGKLDILKRVCAQINKSLLKIINDYEEFSKERSSSLEGSPDEFSNGEELCGVMTISDSPREQDSESQTLDKVYQMKSKPRGYCLIINNHNFAKAREKVPKLHSIRDRNGTHLDAGALTTTFEELHFEIKPHDDCTVEQIYEILKIYQLMDHSNMDCFICCILSHGDKGIIYGTDGQEAPIYELTSQFTGLKCPSLAGKPKVFFIQACQGDNYQKGIPVETDSEEQPYLEMDLSSPQTRYIPDEADFLLGMATVNNCVSYRNPAEGTWYIQSLCQSLRERCPRGDDILTILTEVNYEVSNKDDKKNMGKQMPQPTFTLRKKLVFPSD DIP protein Q14790 original sequence version: 184 DIP protein Q14790 original sequence version: 135 DIP protein Q14790 original sequence version: 136 DIP protein Q14790 original sequence version: 147 DIP protein Q14790 original sequence version: 146 DIP protein Q14790 original sequence version: 170 DIP protein Q14790 original sequence version: 198 DIP protein Q14790-1 original sequence version: 197 DIP protein Q14790 original sequence version: 158 7A5FEAA6B39B582F tr10b_human Tumor necrosis factor receptor superfamily member 10B Death receptor 5 TNF-related apoptosis-inducing ligand receptor 2 UNQ160/PRO186 TNFRSF10B DR5 KILLER TRAILR2 TRICK2 ZTNFR9 protein protein human Homo sapiens Human MEQRGQNAPAASGARKRHGPGPREARGARPGPRVPKTLVLVVAAVLLLVSAESALITQQDLAPQQRAAPQQKRSSPSEGLCPPGHHISEDGRDCISCKYGQDYSTHWNDLLFCLRCTRCDSGEVELSPCTTTRNTVCQCEEGTFREEDSPEMCRKCRTGCPRGMVKVGDCTPWSDIECVHKESGTKHSGEVPAVEETVTSSPGTPASPCSLSGIIIGVTVAAVVLIVAVFVCKSLLWKKVLPYLKGICSGGGGDPERVDRSSQRPGAEDNVLNEIVSILQPTQVPEQEMEVQEPAEPTGVNMLSPGESEHLLEPAEAERSQRRRLLVPANEGDPTETLRQCFDDFADLVPFDSWEPLMRKLGLMDNEIKVAKAEAAGHRDTLYTMLIKWVNKTGRDASVHTLLDALETLGERLAKQKIEDHLLSSGKFMYLEGNADSAMS 60358EAF2A835870 trailmim/dr5 1d TRAIL mimicking peptide 1-dimer peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro WDCLDNRIGRRQCVKL 2xtrailmim/dr5 1m linked through K15 using bis-succunimidyl carboxymehtoxyacetate. trailmim/dr5 3d TRAIL mimicking peptide 3-dimer peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro WDCLDNKIGRRQCVRL 2xtrailmim/dr5 3m linked through K7 using bis-succunimidyl carboxymehtoxyacetate. tnfa_human Tumor necrosis factor TNF TNFA TNFSF2 TNF-alpha Tumor necrosis factor ligand superfamily member 2 Cachectin protein protein human Homo sapiens Human MSTESMIRDVELAEEALPKKTGGPQGSRRCLFLSLFSFLIVAGATTLFCLLHFGVIGPQREEFPRDLSLISPLAQAVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL DIP protein P01375 original sequence version: 198 DIP protein P01375 original sequence version: 151 DIP protein P01375 original sequence version: 156 DIP protein P01375 original sequence version: 186 DIP protein P01375 original sequence version: 201 DIP protein P01375 original sequence version: 174 DIP protein P01375 original sequence version: 211 3DF90F96C9031FFE tr10a_human Tumor necrosis factor receptor superfamily member 10A Death receptor 4 TNF-related apoptosis-inducing ligand receptor 1 TNFRSF10A APO2 DR4 TRAILR1 protein protein human Homo sapiens Human MAPPPARVHLGAFLAVTPNPGSAASGTEAAAATPSKVWGSSAGRIEPRGGGRGALPTSMGQHGPSARARAGRAPGPRPAREASPRLRVHKTFKFVVVGVLLQVVPSSAATIKLHDQSIGTQQWEHSPLGELCPPGSHRSEHPGACNRCTEGVGYTNASNNLFACLPCTACKSDEEERSPCTTTRNTACQCKPGTFRNDNSAEMCRKCSRGCPRGMVKVKDCTPWSDIECVHKESGNGHNIWVILVVTLVVPLLLVAVLIVCCCIGSGCGGDPKCMDRVCFWRLGLLRGPGAEDNAHNEILSNADSLSTFVSEQQMESQEPADLTGVTVQSPGEAQCLLGPAEAEGSQRRRLLVPANGADPTETLMLFFDKFANIVPFDSWDQLMRQLDLTKNEIDVVRAGTAGPGDALYAMLMKWVNKTGRNASIHTLLDALERMEERHAREKIQDLLVDSGKFIYLEDGTGSAVSLE 7E96619D0BDC0CD4 tnr1a_human Tumor necrosis factor receptor superfamily member 1A TNFRSF1A TNFAR TNFR1 p60 Tumor necrosis factor receptor 1 Tumor necrosis factor receptor type I p55 protein protein human Homo sapiens Human MGLSTVPDLLLPLVLLELLVGIYPSGVIGLVPHLGDREKRDSVCPQGKYIHPQNNSICCTKCHKGTYLYNDCPGPGQDTDCRECESGSFTASENHLRHCLSCSKCRKEMGQVEISSCTVDRDTVCGCRKNQYRHYWSENLFQCFNCSLCLNGTVHLSCQEKQNTVCTCHAGFFLRENECVSCSNCKKSLECTKLCLPQIENVKGTEDSGTTVLLPLVIFFGLCLLSLLFIGLMYRYQRWKSKLYSIVCGKSTPEKEGELEGTTTKPLAPNPSFSPTPGFTPTLGFSPVPSSTFTSSSTYTPGDCPNFAAPRREVAPPYQGADPILATALASDPIPNPLQKWEDSAHKPQSLDTDDPATLYAVVENVPPLRWKEFVRRLGLSDHEIDRLELQNGRCLREAQYSMLATWRRRTPRREATLELLGRVLRDMDLLGCLEDIEEALCGPAALPPAPSLLR DIP protein P19438 original sequence version: 135 DIP protein P19438 original sequence version: 150 DIP protein P19438 original sequence version: 177 4CEFBA96D03B8225 tr10d_human Tumor necrosis factor receptor superfamily member 10D Decoy receptor 2 TNF-related apoptosis-inducing ligand receptor 4 TRAIL receptor with a truncated death domain TNFRSF10D DCR2 TRAILR4 TRUNDD UNQ251/PRO288 protein protein human Homo sapiens Human MGLWGQSVPTASSARAGRYPGARTASGTRPWLLDPKILKFVVFIVAVLLPVRVDSATIPRQDEVPQQTVAPQQQRRSLKEEECPAGSHRSEYTGACNPCTEGVDYTIASNNLPSCLLCTVCKSGQTNKSSCTTTRDTVCQCEKGSFQDKNSPEMCRTCRTGCPRGMVKVSNCTPRSDIKCKNESAASSTGKTPAAEETVTTILGMLASPYHYLIIIVVLVIILAVVVVGFSCRKKFISYLKGICSGGGGGPERVHRVLFRRRSCPSRVPGAEDNARNETLSNRYLQPTQVSEQEIQGQELAELTGVTVESPEEPQRLLEQAEAEGCQRRRLLVPVNDADSADISTLLDASATLEEGHAKETIQDQLVGSEKLFYEEDEAGSATSCL 77A93577CAAF2632 trailmim/dr5 2t TRAIL mimicking peptide 2-trimer peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro WDCLDNRIGKRQCVRL 3xtrailmim/dr5 2m linked through an adamantyl core. trailmim/dr5 2m TRAIL mimicking peptide 2-monomer peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro WDCLDNRIGKRQCVRL tnfrsf10a-tnfsf10-3 1 9 unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 17 unspecified role unspecified role bait bait purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction 1b and S1 tnfrsf10b-tnfsf10-4 1 13 unspecified role unspecified role bait bait purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 9 unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 1b and S1 tnfrsf10c-tnfsf10 1 6 unspecified role unspecified role bait bait purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 9 unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 1b and S1 tnfrsf10d-tnfsf10 1 9 unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 19 unspecified role unspecified role bait bait purified purified extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction 1b and S1 tnfrsf10b-1t 1 4 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 13 unspecified role unspecified role bait bait purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction S2 tnfrsf10b-2m 1 21 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 13 unspecified role unspecified role bait bait purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction 1b and S2 tnfrsf10b-3m 1 11 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 13 unspecified role unspecified role bait bait purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction 1b and S2 tnfrsf10b-2d 1 7 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 13 unspecified role unspecified role bait bait purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction S1 and S2 tnfrsf10b-3t 1 10 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 13 unspecified role unspecified role bait bait purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction S1 and S2 tnfrsf10b-2t 1 13 unspecified role unspecified role bait bait purified purified extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 20 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction S2 tnfrsf10b-1m 1 13 unspecified role unspecified role bait bait purified purified extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 8 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 1b and S2 tnfrsf10b-1d 1 14 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 13 unspecified role unspecified role bait bait purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction S1 and S2 tnfrsf10b-3d 1 15 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 13 unspecified role unspecified role bait bait purified purified extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction S1 and S2 tnfsf10-tnfrsf10b-21 1 8 competitor competitor competitor prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 9 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role prey prey purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association 1c tnfsf10-tnfrsf10b-19 1 14 competitor competitor competitor prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 13 unspecified role unspecified role prey prey purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 9 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association 1c tnfsf10-tnfrsf10b-25 1 21 competitor competitor competitor prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 13 unspecified role unspecified role prey prey purified purified extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 9 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association 1c tnfsf10-tnfrsf10b-22 1 7 competitor competitor competitor prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 9 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role prey prey purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association 1c tnfsf10-tnfrsf10b-20 1 20 competitor competitor competitor prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 13 unspecified role unspecified role prey prey purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 9 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association 1c tnfsf10-tnfrsf10b-23 1 11 competitor competitor competitor prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 13 unspecified role unspecified role prey prey purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 9 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association 1c tnfsf10-tnfrsf10b-24 1 15 competitor competitor competitor prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 9 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role prey prey purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association 1c tnfsf10-tnfrsf10b-18 1 10 competitor competitor competitor prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 13 unspecified role unspecified role prey prey purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 9 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association 1c casp8-fadd-13 2 17 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey association association 2b The interaction depends on TNFSF10 (TRAIL) treatment of the cells. casp8-fadd-14 2 5 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey association association 2b The interaction depends on treatment of the cells with TRAILmim/DR5 peptides (1m, 1d, 1t, 2d, 2t, 3m, 3d and 3t). casp8-fadd-15 3 12 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey association association 2b The interaction depends on TNFSF10 (TRAIL) treatment of the cells. tnfrsf1a-tnf-2 1 16 unspecified role unspecified role prey prey purified purified yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 18 unspecified role unspecified role bait bait purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction S1