IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Regulation of cell death and innate immunity by two receptor-like kinases in Arabidopsis. Regulation of cell death and innate immunity by two receptor-like kinases in Arabidopsis. Cell Host Microbe (1931-3128) 2009 imex curation Gao M., Wang X., Wang D., Xu F., Ding X., Zhang Z., Bi D., Cheng Y.T., Chen S., Li X., Zhang Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Interactions found via AP-MS using BRI1-flag are not included because the peptide identified as a prey could be mapped to 3 different proteins. Information for the vectors used in bifc assays was found in PMID:15469500. zhangyuelin@nibs.ac.cn 11-Oct-2012: Contacted by IntAct-Help. Only protein-protein interactions arath-protoplast Arabidopsis thaliana protoplast Protoplast defined as a membrane-bound cell from which the outer wall has been partially or completely removed. bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence Gao M., Wang X., Wang D., Xu F., Ding X., Zhang Z., Bi D., Cheng Y.T., Chen S., Li X., Zhang Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Interactions found via AP-MS using BRI1-flag are not included because the peptide identified as a prey could be mapped to 3 different proteins. Information for the vectors used in bifc assays was found in PMID:15469500. zhangyuelin@nibs.ac.cn Accepted 2012-SEP-24 by MGT 11-Oct-2012: Contacted by IntAct-Help. Cell Host Microbe (1931-3128) 2009 Only protein-protein interactions imex curation Regulation of cell death and innate immunity by two receptor-like kinases in Arabidopsis. Regulation of cell death and innate immunity by two receptor-like kinases in Arabidopsis. Cell Host Microbe (1931-3128) 2009 imex curation Gao M., Wang X., Wang D., Xu F., Ding X., Zhang Z., Bi D., Cheng Y.T., Chen S., Li X., Zhang Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Interactions found via AP-MS using BRI1-flag are not included because the peptide identified as a prey could be mapped to 3 different proteins. Information for the vectors used in bifc assays was found in PMID:15469500. zhangyuelin@nibs.ac.cn 11-Oct-2012: Contacted by IntAct-Help. Only protein-protein interactions arath-seedling Arabidopsis thaliana seedling anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Gao M., Wang X., Wang D., Xu F., Ding X., Zhang Z., Bi D., Cheng Y.T., Chen S., Li X., Zhang Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Interactions found via AP-MS using BRI1-flag are not included because the peptide identified as a prey could be mapped to 3 different proteins. Information for the vectors used in bifc assays was found in PMID:15469500. zhangyuelin@nibs.ac.cn Accepted 2012-SEP-24 by MGT 11-Oct-2012: Contacted by IntAct-Help. Cell Host Microbe (1931-3128) 2009 Only protein-protein interactions imex curation Regulation of cell death and innate immunity by two receptor-like kinases in Arabidopsis. Regulation of cell death and innate immunity by two receptor-like kinases in Arabidopsis. Cell Host Microbe (1931-3128) 2009 imex curation Gao M., Wang X., Wang D., Xu F., Ding X., Zhang Z., Bi D., Cheng Y.T., Chen S., Li X., Zhang Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Interactions found via AP-MS using BRI1-flag are not included because the peptide identified as a prey could be mapped to 3 different proteins. Information for the vectors used in bifc assays was found in PMID:15469500. zhangyuelin@nibs.ac.cn 11-Oct-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay autoradiography autoradiography Gao M., Wang X., Wang D., Xu F., Ding X., Zhang Z., Bi D., Cheng Y.T., Chen S., Li X., Zhang Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Interactions found via AP-MS using BRI1-flag are not included because the peptide identified as a prey could be mapped to 3 different proteins. Information for the vectors used in bifc assays was found in PMID:15469500. zhangyuelin@nibs.ac.cn Accepted 2012-SEP-24 by MGT 11-Oct-2012: Contacted by IntAct-Help. Cell Host Microbe (1931-3128) 2009 Only protein-protein interactions imex curation Regulation of cell death and innate immunity by two receptor-like kinases in Arabidopsis. Regulation of cell death and innate immunity by two receptor-like kinases in Arabidopsis. Cell Host Microbe (1931-3128) 2009 imex curation Gao M., Wang X., Wang D., Xu F., Ding X., Zhang Z., Bi D., Cheng Y.T., Chen S., Li X., Zhang Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Interactions found via AP-MS using BRI1-flag are not included because the peptide identified as a prey could be mapped to 3 different proteins. Information for the vectors used in bifc assays was found in PMID:15469500. zhangyuelin@nibs.ac.cn 11-Oct-2012: Contacted by IntAct-Help. Only protein-protein interactions arath-seedling Arabidopsis thaliana seedling anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Gao M., Wang X., Wang D., Xu F., Ding X., Zhang Z., Bi D., Cheng Y.T., Chen S., Li X., Zhang Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Interactions found via AP-MS using BRI1-flag are not included because the peptide identified as a prey could be mapped to 3 different proteins. Information for the vectors used in bifc assays was found in PMID:15469500. zhangyuelin@nibs.ac.cn Accepted 2012-SEP-24 by MGT 11-Oct-2012: Contacted by IntAct-Help. Cell Host Microbe (1931-3128) 2009 Only protein-protein interactions imex curation serk4_arath Somatic embryogenesis receptor kinase 4 SERK4 BKK1 Protein BAK1-like 1 Somatic embryogenesis receptor-like kinase 2 F13J11.14 At2g13790 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTSSKMEQRSLLCFLYLLLLFNFTLRVAGNAEGDALTQLKNSLSSGDPANNVLQSWDATLVTPCTWFHVTCNPENKVTRVDLGNAKLSGKLVPELGQLLNLQYLELYSNNITGEIPEELGDLVELVSLDLYANSISGPIPSSLGKLGKLRFLRLNNNSLSGEIPMTLTSVQLQVLDISNNRLSGDIPVNGSFSLFTPISFANNSLTDLPEPPPTSTSPTPPPPSGGQMTAAIAGGVAAGAALLFAVPAIAFAWWLRRKPQDHFFDVPAEEDPEVHLGQLKRFTLRELLVATDNFSNKNVLGRGGFGKVYKGRLADGNLVAVKRLKEERTKGGELQFQTEVEMISMAVHRNLLRLRGFCMTPTERLLVYPYMANGSVASCLRERPEGNPALDWPKRKHIALGSARGLAYLHDHCDQKIIHRDVKAANILLDEEFEAVVGDFGLAKLMNYNDSHVTTAVRGTIGHIAPEYLSTGKSSEKTDVFGYGVMLLELITGQKAFDLARLANDDDIMLLDWVKEVLKEKKLESLVDAELEGKYVETEVEQLIQMALLCTQSSAMERPKMSEVVRMLEGDGLAERWEEWQKEEMPIHDFNYQAYPHAGTDWLIPYSNSLIENDYPSGPR 3BFDC6D10E3B9CC7 serk2_arath Somatic embryogenesis receptor kinase 2 SERK2 Somatic embryogenesis receptor-like kinase 2 F23M19.11 At1g34210 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGRKKFEAFGFVCLISLLLLFNSLWLASSNMEGDALHSLRANLVDPNNVLQSWDPTLVNPCTWFHVTCNNENSVIRVDLGNADLSGQLVPQLGQLKNLQYLELYSNNITGPVPSDLGNLTNLVSLDLYLNSFTGPIPDSLGKLFKLRFLRLNNNSLTGPIPMSLTNIMTLQVLDLSNNRLSGSVPDNGSFSLFTPISFANNLDLCGPVTSRPCPGSPPFSPPPPFIPPPIVPTPGGYSATGAIAGGVAAGAALLFAAPALAFAWWRRRKPQEFFFDVPAEEDPEVHLGQLKRFSLRELQVATDSFSNKNILGRGGFGKVYKGRLADGTLVAVKRLKEERTPGGELQFQTEVEMISMAVHRNLLRLRGFCMTPTERLLVYPYMANGSVASCLRERPPSQLPLAWSIRQQIALGSARGLSYLHDHCDPKIIHRDVKAANILLDEEFEAVVGDFGLARLMDYKDTHVTTAVRGTIGHIAPEYLSTGKSSEKTDVFGYGIMLLELITGQRAFDLARLANDDDVMLLDWVKGLLKEKKLEMLVDPDLQSNYTEAEVEQLIQVALLCTQSSPMERPKMSEVVRMLEGDGLAEKWDEWQKVEVLRQEVELSSHPTSDWILDSTDNLHAMELSGPR DIP protein Q9XIC7 original sequence version: 125 23F6C0DC3717C74F bak1_arath BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 BAK1 SERK3 At4g33430 F17M5.190 Somatic embryogenesis receptor-like kinase 3 ELG Somatic embryogenesis receptor kinase 3 Protein ELONGATED protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MERRLMIPCFFWLILVLDLVLRVSGNAEGDALSALKNSLADPNKVLQSWDATLVTPCTWFHVTCNSDNSVTRVDLGNANLSGQLVMQLGQLPNLQYLELYSNNITGTIPEQLGNLTELVSLDLYLNNLSGPIPSTLGRLKKLRFLRLNNNSLSGEIPRSLTAVLTLQVLDLSNNPLTGDIPVNGSFSLFTPISFANTKLTPLPASPPPPISPTPPSPAGSNRITGAIAGGVAAGAALLFAVPAIALAWWRRKKPQDHFFDVPAEEDPEVHLGQLKRFSLRELQVASDNFSNKNILGRGGFGKVYKGRLADGTLVAVKRLKEERTQGGELQFQTEVEMISMAVHRNLLRLRGFCMTPTERLLVYPYMANGSVASCLRERPESQPPLDWPKRQRIALGSARGLAYLHDHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMDYKDTHVTTAVRGTIGHIAPEYLSTGKSSEKTDVFGYGVMLLELITGQRAFDLARLANDDDVMLLDWVKGLLKEKKLEALVDVDLQGNYKDEEVEQLIQVALLCTQSSPMERPKMSEVVRMLEGDGLAERWEEWQKEEMFRQDFNYPTHHPAVSGWIIGDSTSQIENEYPSGPR DIP protein Q94F62 original sequence version: 119 DIP protein Q94F62 original sequence version: 129 DIP protein Q94F62 original sequence version: 94 DIP protein Q94F62 original sequence version: 100 DIP protein Q94F62 original sequence version: 139 DIP protein Q94F62 original sequence version: 121 DIP protein Q94F62 original sequence version: 141 DIP protein Q94F62 original sequence version: 113 547B3F38FB46E1DD serk1_arath Somatic embryogenesis receptor kinase 1 SERK1 Somatic embryogenesis receptor-like kinase 1 F14O23.21 F14O23_24 At1g71830 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MESSYVVFILLSLILLPNHSLWLASANLEGDALHTLRVTLVDPNNVLQSWDPTLVNPCTWFHVTCNNENSVIRVDLGNAELSGHLVPELGVLKNLQYLELYSNNITGPIPSNLGNLTNLVSLDLYLNSFSGPIPESLGKLSKLRFLRLNNNSLTGSIPMSLTNITTLQVLDLSNNRLSGSVPDNGSFSLFTPISFANNLDLCGPVTSHPCPGSPPFSPPPPFIQPPPVSTPSGYGITGAIAGGVAAGAALLFAAPAIAFAWWRRRKPLDIFFDVPAEEDPEVHLGQLKRFSLRELQVASDGFSNKNILGRGGFGKVYKGRLADGTLVAVKRLKEERTPGGELQFQTEVEMISMAVHRNLLRLRGFCMTPTERLLVYPYMANGSVASCLRERPPSQPPLDWPTRKRIALGSARGLSYLHDHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMDYKDTHVTTAVRGTIGHIAPEYLSTGKSSEKTDVFGYGIMLLELITGQRAFDLARLANDDDVMLLDWVKGLLKEKKLEMLVDPDLQTNYEERELEQVIQVALLCTQGSPMERPKMSEVVRMLEGDGLAEKWDEWQKVEILREEIDLSPNPNSDWILDSTYNLHAVELSGPR DIP protein Q94AG2 original sequence version: 112 DIP protein Q94AG2 original sequence version: 115 98F6B69126DB664A y5838_arath Probably inactive leucine-rich repeat receptor-like protein kinase At5g48380 K23F3.10 MJE7.1 At5g48380 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MMMGRLVFVIWLYNCLCLLLLSSLVDADQANIDCLRTFKSQVEDPNRYLSTWVFGNETAGYICKFSGVTCWHDDENRVLSIKLSGYGLRGVFPPAVKLCADLTGLDLSRNNFSGPLPANISTLIPLVTILDLSYNSFSGEIPMLISNITFLNTLMLQHNQFTGTLPPQLAQLGRLKTFSVSDNRLVGPIPNFNQTLQFKQELFANNLDLCGKPLDDCKSASSSRGKVVIIAAVGGLTAAALVVGVVLFFYFRKLGAVRKKQDDPEGNRWAKSLKGQKGVKVFMFKKSVSKMKLSDLMKATEEFKKDNIIATGRTGTMYKGRLEDGSLLMIKRLQDSQRSEKEFDAEMKTLGSVKNRNLVPLLGYCVANKERLLMYEYMANGYLYDQLHPADEESFKPLDWPSRLKIAIGTAKGLAWLHHSCNPRIIHRNISSKCILLTAEFEPKISDFGLARLMNPIDTHLSTFVNGEFGDFGYVAPEYSRTMVATPKGDVYSFGVVLLELVTGQKATSVTKVSEEKAEEENFKGNLVEWITKLSSESKLQEAIDRSLLGNGVDDEIFKVLKVACNCVLPEIAKQRPTMFEVYQLLRAIGESYNFTADDDILIPSESGEGDFIEELIVAR FF2CD5BCF319D385 y5838 3 9 enzyme enzyme putative self putative self purified purified Q9ASS4:p.Lys331Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K E Wed May 02 14:56:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys331glu' Tue Jun 30 20:20:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys331Glu' n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range kd domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation true S6a bak1-y5838 1 7 unspecified role unspecified role neutral component neutral component c-terminal nyfp nYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range This tag goes right after the myc tag on the c-terminus. c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range This tag goes on the c-terminus, right before the YFP-n tag. 9 unspecified role unspecified role neutral component neutral component c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range This tag goes on the c-terminus, just before the YFP-c tag. c-terminal cyfp cYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range This tag goes right after the HA tag on the c-terminus. physical association physical association 2b and S15 serk4-y5838 1 5 unspecified role unspecified role neutral component neutral component c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range This tag goes on the c-terminus, right before the YFP-n tag. c-terminal nyfp nYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range This tag goes right after the myc tag on the c-terminus. 9 unspecified role unspecified role neutral component neutral component c-terminal cyfp cYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range This tag goes right after the HA tag on the c-terminus. c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range This tag goes on the c-terminus, just before the YFP-c tag. physical association physical association 2b serk1-y5838 1 9 unspecified role unspecified role neutral component neutral component c-terminal cyfp cYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range This tag goes right after the HA tag on the c-terminus. c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range This tag goes on the c-terminus, just before the YFP-c tag. 8 unspecified role unspecified role neutral component neutral component c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range This tag goes on the c-terminus, right before the YFP-n tag. c-terminal nyfp nYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range This tag goes right after the myc tag on the c-terminus. physical association physical association 2b serk2-y5838 1 6 unspecified role unspecified role neutral component neutral component c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range This tag goes on the c-terminus, right before the YFP-n tag. c-terminal nyfp nYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range This tag goes right after the myc tag on the c-terminus. 9 unspecified role unspecified role neutral component neutral component c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range This tag goes on the c-terminus, just before the YFP-c tag. c-terminal cyfp cYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range This tag goes right after the HA tag on the c-terminus. physical association physical association 2b bak1-y5838-1 2 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey physical association physical association 2c y5838-bak1 4 9 unspecified role unspecified role bait bait c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 7 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2d bak1-y5838-2 4 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 2e