IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ nhl-2 Modulates microRNA activity in Caenorhabditis elegans. nhl-2 Modulates microRNA activity in Caenorhabditis elegans. Cell (0092-8674) 2009 imex curation Hammell CM., Lubin I., Boag PR., Blackwell TK., Ambros V. Apoptosis - Interactions involving proteins with a function related to apoptosis victor.ambros@umassmed.edu 09-April-2009: Contacted by JYOTI. C. elegans specific cDNA libraries (Clonetech) davet Only protein-protein interactions 4 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Hammell CM., Lubin I., Boag PR., Blackwell TK., Ambros V. Apoptosis - Interactions involving proteins with a function related to apoptosis victor.ambros@umassmed.edu Accepted 2009-APR-25 by DAVET. 09-April-2009: Contacted by JYOTI. C. elegans specific cDNA libraries (Clonetech) Cell (0092-8674) 2009 davet Only protein-protein interactions 4 imex curation nhl-2 Modulates microRNA activity in Caenorhabditis elegans. nhl-2 Modulates microRNA activity in Caenorhabditis elegans. Cell (0092-8674) 2009 imex curation Hammell CM., Lubin I., Boag PR., Blackwell TK., Ambros V. Apoptosis - Interactions involving proteins with a function related to apoptosis victor.ambros@umassmed.edu 09-April-2009: Contacted by JYOTI. C. elegans specific cDNA libraries (Clonetech) davet Only protein-protein interactions 4 caeel-larva Immature form of the adult worm larva Immature form of an organism anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hammell CM., Lubin I., Boag PR., Blackwell TK., Ambros V. Apoptosis - Interactions involving proteins with a function related to apoptosis victor.ambros@umassmed.edu Accepted 2009-APR-25 by DAVET. 09-April-2009: Contacted by JYOTI. C. elegans specific cDNA libraries (Clonetech) Cell (0092-8674) 2009 davet Only protein-protein interactions 4 imex curation nhl-2 Modulates microRNA activity in Caenorhabditis elegans. nhl-2 Modulates microRNA activity in Caenorhabditis elegans. Cell (0092-8674) 2009 imex curation Hammell CM., Lubin I., Boag PR., Blackwell TK., Ambros V. Apoptosis - Interactions involving proteins with a function related to apoptosis victor.ambros@umassmed.edu 09-April-2009: Contacted by JYOTI. C. elegans specific cDNA libraries (Clonetech) davet Only protein-protein interactions 4 caeel-larva Immature form of the adult worm larva Immature form of an organism anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hammell CM., Lubin I., Boag PR., Blackwell TK., Ambros V. Apoptosis - Interactions involving proteins with a function related to apoptosis victor.ambros@umassmed.edu Accepted 2009-APR-25 by DAVET. 09-April-2009: Contacted by JYOTI. C. elegans specific cDNA libraries (Clonetech) Cell (0092-8674) 2009 davet Only protein-protein interactions 4 imex curation nhl-2 Modulates microRNA activity in Caenorhabditis elegans. nhl-2 Modulates microRNA activity in Caenorhabditis elegans. Cell (0092-8674) 2009 imex curation Hammell CM., Lubin I., Boag PR., Blackwell TK., Ambros V. Apoptosis - Interactions involving proteins with a function related to apoptosis victor.ambros@umassmed.edu 09-April-2009: Contacted by JYOTI. C. elegans specific cDNA libraries (Clonetech) davet Only protein-protein interactions 4 caeel Caenorhabditis elegans fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Hammell CM., Lubin I., Boag PR., Blackwell TK., Ambros V. Apoptosis - Interactions involving proteins with a function related to apoptosis victor.ambros@umassmed.edu Accepted 2009-APR-25 by DAVET. 09-April-2009: Contacted by JYOTI. C. elegans specific cDNA libraries (Clonetech) Cell (0092-8674) 2009 davet Only protein-protein interactions 4 imex curation q17740_caeel ain-1 C06G1.4 CELE_C06G1.4 protein protein caeel Caenorhabditis elegans MESLAKNFNNISIDDAAWHPNDQQPVWGNNGLQQQRVWAPPVPQPQFPNRSPWQQESNDIEPNVNEALVGLSGTGSSSQSQWGGIQNTDYEKQIWGDPPLSDVQYPLQPHASFGELNSLGGQSAPWSIGSQPAHPVWSNGGMGKESDDFWKQQQPQQHQQYPMPLLQGAWNTGMRHGPPGQGQRSGPMMQQQQDYQGGWGGPNNMGGNKQMNRPWGQDSNRGMSGMPPRNGRPNHNQGRYSGNMGGGVDVSVPPPMDMHMGGGMGGMRNMGVAPNSGHGSWKNNNAGGGQGGHGGQRNYNNRGGMGHSGSQSGGGSNMWNSTGNNGSGGSMQQFMGGDQQYNNPFPIGGAADDLTLTVWHDPNGELKKWQRDTGVSYWGDPEKQTDRTISLWIVGEGADEDLETALNRCPVPQKKGEDNQRFPFPVPAKRPIVVTGWGELPENDPNNPSKSESTIFDENNRWNDLTTEQNPWYLPNQHTSTFSSDNTTGSWVQGGTIPLNEPGTNQHVAVAEMLKNAVDKGYLDQSVTMMANLPPMVLQYVNMLLVKIPALDSVENELKQIIESSLPEDVKEVDPQNQQKYMNDSQKLEHNRLIIEVTTAKIEVQEYSKKVNRALMDAGIVQPQEQRAPASTEDYHYSFLE 2BE1655143A10010 o16720_caeel alg-2 CELE_T07D3.7 T07D3.7 protein protein caeel Caenorhabditis elegans MFPLPVHNGPRLGKLSIFEMPGDSLTSSSFMPDGGAETSSSSQLGGSAHGAIGTKPDAGVQFQCPVRPNHGVEGRSILLRANHFAVRIPGGSVQHYQIDVFPDKCPRRVNREVIGCLISSFSKYFTNIRPVYDGKRNMYTREPLPIGTEPMNFEVTLPGDSAVERKFSVTMKWIGQVCLSALDDAMEGRVRQVPHEAVQSIDVILRHLPSLKYTPVGRSFFTPPGVMKPGMQMHQESKLGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYRAMPVIEFVAEVLELPVQALAERRALSDAQRVKFTKEIRGLKIEITHCGAVRRKYRVCNVTRRPAQTQTFPLQLETGQTIECTVAKYFFDKYRIQLKYPHLPCLQVGQEQKHTYLPPEVCDIVPGQRCLKKLTDVQTSTMIKATARSAPEREREICKLVSKAELSADPFAHEFGITINPAMTEVKGRVLSAPKLLYGGRHRATTALPNQGVWDMRGKQFHTGMEVRTWAIACFAQQSHVKENDLRMFTTQLQRISTDAGMPIIGTPMFCKYASGVEQVEPMFKYLKQTYSAIQLIVVVLPGKTPIYAEVKRVGDTVLGIATQCVQAKNAIRTTPQTLSNLCLKMNVKLGGVNSILLPNVRPRIFNEPVIFLGCDITHPAAGDTRKPSIAAVVGSMDAHPSRYAATVRVQQHRQEIITDLTYMVRELLVQFYRNTRFKPARIVVYRDGVSEGQLFNVLQYELRAIREACVMLESGYQPGITFIAVQKRHHTRLFAADKADQVGKAFNIPPGTTVDVGITHPTEFDFFLCSHAGIQGTSRPSHYHVLWDDNDLTADELQQLTYQMCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDRDHGSGEEGSQPSGTSSEDTTLSSMAKAVQVHPDSNNVMYFA EFED1EE40E6696B4 g5egr6_caeel alg-1 CELE_F48F7.1 F48F7.1 protein protein caeel Caenorhabditis elegans MSGGPQYLPGVMNSTIQQQPQSATSSFLPSGPISSTSTSSQVVPTSGATQQPPFPSAQAAASTALQNDLEEIFNSPPTQPQTFSDVPQRQAGSLAPGVPIGNTSVSIGEPANTLGGGLPGGAPGQLPGGNQSGIQFQCPRRPNHGVEGRSILLRANHFAVRIPGGTIQHYQVDVTPDKCPRRVNREIISCLISAFSKYFTNIRPVYDGKRNMYTREPLPIGRERMDFDVTLPGDSAVERQFSVSLKWVGQVSLSTLEDAMEGRVRQVPFEAVQAMDVILRHLPSLKYTPVGRSFFSPPVPNASGVMAGSCPPQASGAVAGGAHSAGQYHAESKLGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYRSMPVIEFIAEVLELPVQALAERRALSDAQRVKFTKEIRGLKIEITHCGQMRRKYRVCNVTRRPAQTQTFPLQLETGQTIECTVAKYFYDKYRIQLKYPHLPCLQVGQEQKHTYLPPEVCNIVPGQRCIKKLTDVQTSTMIKATARSAPEREREISNLVRKAEFSADPFAHEFGITINPAMTEVKGRVLSAPKLLYGGRTRATALPNQGVWDMRGKQFHTGIDVRVWAIACFAQQQHVKENDLRMFTNQLQRISNDAGMPIVGNPCFCKYAVGVEQVEPMFKYLKQNYSGIQLVVVVLPGKTPVYAEVKRVGDTVLGIATQCVQAKNAIRTTPQTLSNLCLKMNVKLGGVNSILLPNVRPRIFNEPVIFFGCDITHPPAGDSRKPSIAAVVGSMDAHPSRYAATVRVQQHRQEIISDLTYMVRELLVQFYRNTRFKPARIVVYRDGVSEGQFFNVLQYELRAIREACMMLERGYQPGITFIAVQKRHHTRLFAVDKKDQVGKAYNIPPGTTVDVGITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNNLTADELQQLTYQMCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDREHDSGEGSQPSGTSEDTTLSNMARAVQVHPDANNVMYFA FB4C9227B25F44F3 q19818_caeel nhl-2 F26F4.7 CELE_F26F4.7 protein protein caeel Caenorhabditis elegans MKKAADRPTSAVNGRHESPATSTNNTQNSGTPSLNGQDPLRSTSSSPLFGTEPLPEDDHASCPYCQQDFRKPRVLDCLHSMCEDCIIAQLDGHLQAKSAAIEEKKRATAAMLDCELETPKVKERPTPPGVIRCPICSQESHVGNDVRYVHAMLLDFVRLAEMKEVEVEKMVAHHRKPCTACKSEQEAIAFCDQCSSDLCENCTTAHTVMNLFQGHTVTTFADLRQPGAHPELRQVMCSTHNQHMRYLCAACETAACKQCLEVDHLNHKIIDINELVINAIREDVTSTVDRVEKKYNNSVADMNSLPDKSQQLNEQYEIAKYRVDKHYEELRNSIEEAYQKSVNDLEEARIRQESNIDEMYRKSQVNEARVHDAINFTRRLLTKGNGLELVVSRKKVIQQFGNLSHAIPSHGHQVELEFYTPSKKQIDHFVHQLTGTVIGRVIQPTVKDVGNATAASTDRSERGGSQPGGGRSSSAANPSQQDEWGRPVYSQNPQLGAIGGERKKNTQNGTNGTRGDSNAFGGWPPSSHPPESTTPPPVTGPQTMITGAPTQTAAPIVSQQQLSNLPNAAAMAAAAAGMRPDLVALAANHMDLAALTNGSTNGSQTPLPESIFPAMWNARQGLPAVATPTTTGLSTNASAAYMQQKLAAAQLQALASHRLNPQIMSMNNLRTNQTIQEFLAMNAMGNLSLNPQLTSAAAAALAATAAANSAVTTTASDVGQSQNSANAQRVSDLKVHSVFGTSQQGSTIRELHCPSGFCLSDTDDILIADTNNHRVVVCGPPHPWKIGRPGTDDGQLCFPRKVIALRGEAVRYVVLDKGGDGKTRAQIFEARGEFVKRLNMMALVPRGGIEVSAAAATPNGQLLLVDTAGFVYSIDVDAPRVTFWFDASTQLGEASDVAMFDNLIYITDFKHHCVQVYTSEGKFIRKMGEPSQTPYPIGIDVSKAGEVLVADTHGNHLHVVVFSPEGQHIHSFTHNEFRLSRCVGLRIAKSGHIVTLCKHNHTLFVFKPLILPNGLPVPTPVPHATSLISKFQ FF96EB0B510F6718 cgh1_caeel ATP-dependent RNA helicase cgh-1 C07H6.5 Conserved germline helicase 1 cgh-1 protein protein caeel Caenorhabditis elegans MSGAEQQQIVPANNGDENWKAGLNLPAKDRRFKTADVTDTKGVEFEDFCLGRDLLMGIFEKGWEKPSPIQEASIGVALTGQDILARAKNGTGKTGAYCIPVIEKIQPALKAIQAMVIVPTRELALQTSQICVELSKHIQLKVMVTTGGTDLRDDIMRLNGTVHLVIATPGRILDLMEKGVAKMEHCKTLVLDEADKLLSQDFQGILDRLINFLPKERQVMLYSATFPNTVTSFMQKHMHKPYEINLMEELTLLGVTQYYAFVQEKQKVHCLNTLFRKLQINQSIIFCNSTQRVELLAKKITEIGYSCYYIHSKMAQNHRNRVFHDFRQGNCRNLVCSDLLTRGIDIQAVNVVINFDFPRNAETYLHRIGRSGRFGHLGVAINLITYEDRHTLRRIEQELRTRIEPIPKTVDPKLYVADQQLVDAADETTA 0C31C5597DD8B5D4 nhl2-cgh1-1 1 8 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association NHL-2 Physically Interacts with the DEAD-Box Protein CGH-1 Fig. 4B, 4C nhl2-cgh1-2 4 8 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization CGH-1::GFP and dsRED::NHL-2 colocalize in somatic tissues to several punctate, cytoplasmic bodies. Fig. 4D, 4CE alg1-cgh1-1 3 9 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey association association Immunoprecipitation of GFP::NHL-2 from extracts of larvae coprecipitates ALG-1 and ALG-2. Fig. 6B nhl2-cgh1-4 2 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey association association Immunoprecipitation of CGH-1 coprecipitates ALG-1/2, AIN-1,and NHL-2. Fig. 6C, 6D RNase treatment of immunoprecipitated material reduces CGH-1 binding to core miRISC components but does not dramatically affect its interaction with NHL-2. Coimmunoprecipitation of ALG-1/2 and AIN-1 with CGH-1 is not affected in the absence of nhl-2.