IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ ACF7 regulates cytoskeletal-focal adhesion dynamics and migration and has ATPase activity. ACF7 regulates cytoskeletal-focal adhesion dynamics and migration and has ATPase activity. Cell (0092-8674) 2008 imex curation Wu X., Kodama A., Fuchs E. Apoptosis - Interactions involving proteins with a function related to apoptosis Source and details of PAK and RTKN proteins taken from manufacturer's website, from Rho, Rac, Cdc42 activation assay kits. fuchslb@rockefeller.edu 16-Dec-2008: Contacted by JYOTI. davet Only protein-protein interactions 5 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Wu X., Kodama A., Fuchs E. Apoptosis - Interactions involving proteins with a function related to apoptosis Source and details of PAK and RTKN proteins taken from manufacturer's website, from Rho, Rac, Cdc42 activation assay kits. fuchslb@rockefeller.edu Accepted 2008-DEC-20 by DAVET. 16-Dec-2008: Contacted by JYOTI. Cell (0092-8674) 2008 davet Only protein-protein interactions 5 imex curation ACF7 regulates cytoskeletal-focal adhesion dynamics and migration and has ATPase activity. ACF7 regulates cytoskeletal-focal adhesion dynamics and migration and has ATPase activity. Cell (0092-8674) 2008 imex curation Wu X., Kodama A., Fuchs E. Apoptosis - Interactions involving proteins with a function related to apoptosis Source and details of PAK and RTKN proteins taken from manufacturer's website, from Rho, Rac, Cdc42 activation assay kits. fuchslb@rockefeller.edu 16-Dec-2008: Contacted by JYOTI. davet Only protein-protein interactions 5 mouse-epithelium Mus musculus epithelium Epithelium [epithelial cells] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wu X., Kodama A., Fuchs E. Apoptosis - Interactions involving proteins with a function related to apoptosis Source and details of PAK and RTKN proteins taken from manufacturer's website, from Rho, Rac, Cdc42 activation assay kits. fuchslb@rockefeller.edu Accepted 2008-DEC-20 by DAVET. 16-Dec-2008: Contacted by JYOTI. Cell (0092-8674) 2008 davet Only protein-protein interactions 5 imex curation ACF7 regulates cytoskeletal-focal adhesion dynamics and migration and has ATPase activity. ACF7 regulates cytoskeletal-focal adhesion dynamics and migration and has ATPase activity. Cell (0092-8674) 2008 imex curation Wu X., Kodama A., Fuchs E. Apoptosis - Interactions involving proteins with a function related to apoptosis Source and details of PAK and RTKN proteins taken from manufacturer's website, from Rho, Rac, Cdc42 activation assay kits. fuchslb@rockefeller.edu 16-Dec-2008: Contacted by JYOTI. davet Only protein-protein interactions 5 in vitro In vitro atpase assay atpase assay western blot western blot Immuno blot Immuno blot Wu X., Kodama A., Fuchs E. Apoptosis - Interactions involving proteins with a function related to apoptosis Author has been contacted for exact details of construct and also for species of origin of EB1 used in Supp. Fig. 8. Source and details of PAK and RTKN proteins taken from manufacturer's website, from Rho, Rac, Cdc42 activation assay kits. fuchslb@rockefeller.edu Accepted 2008-DEC-20 by DAVET. 16-Dec-2008: Contacted by JYOTI. Cell (0092-8674) 2008 davet Only protein-protein interactions 5 imex curation ACF7 regulates cytoskeletal-focal adhesion dynamics and migration and has ATPase activity. ACF7 regulates cytoskeletal-focal adhesion dynamics and migration and has ATPase activity. Cell (0092-8674) 2008 imex curation Wu X., Kodama A., Fuchs E. Apoptosis - Interactions involving proteins with a function related to apoptosis Source and details of PAK and RTKN proteins taken from manufacturer's website, from Rho, Rac, Cdc42 activation assay kits. fuchslb@rockefeller.edu 16-Dec-2008: Contacted by JYOTI. davet Only protein-protein interactions 5 mouse-epithelium Mus musculus epithelium Epithelium [epithelial cells] anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Wu X., Kodama A., Fuchs E. Apoptosis - Interactions involving proteins with a function related to apoptosis Author has been contacted for exact details of construct and also for species of origin of EB1 used in Supp. Fig. 8. Source and details of PAK and RTKN proteins taken from manufacturer's website, from Rho, Rac, Cdc42 activation assay kits. fuchslb@rockefeller.edu Accepted 2008-DEC-20 by DAVET. 16-Dec-2008: Contacted by JYOTI. Cell (0092-8674) 2008 davet Only protein-protein interactions 5 imex curation clap2_mouse CLIP-associating protein 2 Cytoplasmic linker-associated protein 2 Clasp2 Kiaa0627 protein protein mouse Mus musculus Mouse MRRLICKRICDYKSFDDEESVDGNRPSSAASAFKVPAPKTPGNPVSSARKPGSAGGPKVGGPSKEGGAGAVDEDDFIKAFTDVPSVQIYSSRELEETLNKIREILSDDKHDWDQRANALKKIRSLLVAGAAQYDCFFQHLRLLDGALKLSAKDLRSQVVREACITVAHLSTVLGNKFDHGAEAIVPTLFNLVPNSAKVMATSGCAAIRFIIRHTHVPRLIPLITSNCTSKSVPVRRRSFEFLDLLLQEWQTHSLERHAAVLVETIKKGIHDADAEARVEARKTYMGLRNHFPGEAETLYNSLEPSYQKSLQTYLKSSGSVASLPQSDRSSSSSQESLNRPFSSKWSTANPSTVAGRVSVGGSKANPLPGSLQRSRSDIDVNAAAGAKAHHAAGQAVRSGRLGAGALNPGSYASLEDTSDKMDGTASDDGRVRAKLSTPLVAVGNAKTDSRGRSRTKMVSQSQPGSRSGSPGRVLTTTALSTVSSGAQRVLVNSASAQKRSKIPRSQGCSREASPSRLSVARSSRIPRPSVSQGCSREASRESSRDTSPVRSFQPLGPGYGISQSSRLSSSVSAMRVLNTGSDVEEAVADALLLGDIRTKKKPARRRYESYGMHSDDDANSDASSACSERSYSSRNGSIPTYMRQTEDVAEVLNRCASSNWSERKEGLLGLQNLLKNQRTLSRIELKRLCEIFTRMFADPHGKVFSMFLETLVDFIQVHKDDLQDWLFVLLTQLLKKMGADLLGSVQAKVQKALDITRESFPNDLQFNILMRFTVDQTQTPSLKVKVAILKYIETLAKQMDPRDFTNSSETRLAVSRVITWTTEPKSSDVRKAAQSVLISLFELNTPEFTMLLGALPKTFQDGATKLLHNHLRNTGNGTQSSMGSPLTRPTPRSPANWSSPLTSPTNTSQNTLSPSAFDYDTENMNSEDIYSSLRGVTEAIQNFSFRSQEDMSEPVRRDPKKEDGDTICSGPGMSDPRAGGDAADGSQPALDNKASLLHSMPLHSSPRSRDYNPYNYSDSISPFNKSALKEAMFDDDADQFPDDLSLDHSDLVAELLKELSNHNERIEERKIALYELMKLTQEESFSVWDEHFKTILLLLLETLGDKEPTIRALALKVLKEILRHQPARFKNYAELTVMKTLEAHKDPHKEVVRSAEEAASVLATSISPEQCIKVLCPIIQTADYPINLAAIKMQTKVIERVSKETLNMLLPEIMPGLIQGYDNSESSVRKACVFCLVAVHAVIGDELKPHLSQLTGSKMKLLNLYIKRAQTGSAGADPTADVSGQS 5B208D8F4E90CB93 clap1_mouse CLIP-associating protein 1 Clasp1 Cytoplasmic linker-associated protein 1 Kiaa0622 protein protein mouse Mus musculus Mouse MEPRMESCLAQVLQKDVGKRLQVGQELIDYFSDRQKSADLEHDQTLLDKLVDGLATSWVNSSNYKVVLLGMDILSALVTRLQDRFKAQIGTVLPSLIDRLGDAKDSVREQDQTLLLKIMDQAANPQYVWDRMLGGFKHKNFRTREGICLCLIATLNASGAQTLTLSKIVPHICNLLGDPNSQVRDAAINSLVEIYRHVGERVRADLSKKGLPQSRLNVIFTKFDEVQKSGNMIQSANEKNFDDEDSVDGNRPSSASSSSSKAPSSSRRNVNLGTTRRLMSSSLGSKSSAAKEGAGAVDEEDFIKAFDDVPVVQIYSSRDLEESINKIREILSDDKHDWEQRVNALKKIRSLLLAGAAEYDNFFQHLRLLDGAFKLSAKDLRSQVVREACITLGHLSSVLGNKFDHGAEAIMPTIFNLIPNSAKIMATSGVVAVRLIIRHTHIPRLIPVITSNCTSKSVAVRRRCFEFLDLLLQEWQTHSLERHISVLAETIKKGIHDADSEARIEARKCYWGFHSHFSREAEHLYHTLESSYQKALQSHLKNSDSIVSLPQSDRSSSSSQESLNRPLSAKRSPTGSTASRGSTVSTKSVSTTGSLQRSRSDIDVNAAASAKSKVSSSSGSPAFSSAAALPPGSYASLGRIRTRRQSSGSTTNVASTPDSRGRSRAKVVSQSQRSRSANPAGAGSRSSSPGKLLGSGLAGGSSRGPPVTPSSEKRSKIPRSQGCSRETSPNRIGLARSSRIPRPSMSQGCSRDTSRESSRDTSPARGFTPLDRFGLGQSGRIPGSVNAMRVLSTSTDLEAAVADALKKPVRRRYEPYGMYSDDDANSDASSVCSERSYGSRNGGIPHYLRQTEDVAEVLNHCASSNWSERKEGLLGLQNLLKSQRTLSRVELKRLCEIFTRMFADPHSKRVFSMFLETLVDFIIIHKDDLQDWLFVLLTQLLKKMGADLLGSVQAKVQKALDVTRDSFPFDQQFNILMRFIVDQTQTPNLKVKVAILKYIESLARQMDPTDFVNSSETRLAVSRIITWTTEPKSSDVRKAAQIVLISLFELNTPEFTMLLGALPKTFQDGATKLLHNHLKNSSNTGVGSPSNTIGRTPSRHPSSRTSPLTSPTNCSHGGLSPSRLWGWSADGLSKPPPPFSQPNSIPTAPSHKTLRRSYSPSMLDYDTENLNSEEIYSSLRGVTEAIEKFSFRSQEDLNEPIKRDGKKDCDIVSRDGGAASPATEGRGGSEIEGGRMALDNKTSLLNTQPPRAFPGPRAREYNPYPYSDTINTYDKTALKEAVFDDDMEQLRDVPIDHSDLVADLLKELSNHNERVEERKGALLELLKITREDSLGVWEEHFKTILLLLLETLGDKDHSIRALALRVLREILRNQPARFKNYAELTIMKTLEAHKDSHKEVVRAAEEAASTLASSIHPEQCIKVLCPIIQTADYPINLAAIKMQTKVVERITKESLLQLLVDIIPGLLQGYDNTESSVRKASVFCLVAIYSVIGEDLKPHLAQLTGSKMKLLNLYIKRAQTTNSNSSSSSDVSTHS 5EE40CCE493C64D2 atp ATP Adenosine 5'-triphosphate Adenosine triphosphate ADENOSINE-5'-TRIPHOSPHATE Adephos Adetol Adynol Atipi ATP Atriphos Cardenosine Fosfobion Glucobasin H4atp Myotriphos Triadenyl Triphosphaden adenosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) macf1_mouse Microtubule-actin cross-linking factor 1 Actin cross-linking family 7 Macf1 Acf7 Aclp7 Macf protein protein mouse Mus musculus Mouse MSSSDEETLSERSCRSERSCRSERSYRSERSGSLSPCPPGDTLPWNLPLHEQKKRKSQDSVLDPAERAVVRVADERDRVQKKTFTKWVNKHLMKVRKHINDLYEDLRDGHNLISLLEVLSGIKLPREKGRMRFHRLQNVQIALDFLKQRQVKLVNIRNDDITDGNPKLTLGLIWTIILHFQISDIYISGESGDMSAKEKLLLWTQKVTAGYTGVKCTNFSSCWSDGKMFNALIHRYRPDLVDMERVQVQSNRENLEQAFEVAERLGVTRLLDAEDVDVPSPDEKSVITYVSSIYDAFPKVPEGGEGISATEVDSRWQEYQSRVDSLIPWIRQHTILMSDKSFPQNPVELKALYNQYIHFKETEILAKEREKGRIKELYKLLEVWIEFGRIKLPQGYHPNHVEEEWGKLIVEMLEREKSLRPAVERLELLLQIANKIQNGALNCEEKLTLAKNTLQADAAHLESGQPVQCESDVIMYIQECEGLIRQLQVDLQILRDEKYYQLEELAFRVMRLQDELVTLRLECTNLYRKGHFSSLELVPPSTLTTTHLKAEPLNKTTHSSSTSWFRKPMTRTELVSISSSEDEGNLRFVYELLSWVEEMQMKLERAEWGNDLPSVELQLETQQHIHTSVEELGSSVKEARLYEGKMSQNFHTSYVETLGKLETQYCKLKETSSFRMRHLQSLHKFVSRATAELIWLNGKEEEELACDWSDSNPNISAKKTYFSELTMELEGKQDVFRSLQDTAEVLSLENHPAKQTVEAYSAAVQSQLQWMKQLCLCVEQHVKENAAYFQFFSDARDLESFLRNLQDSIKRKYTCDRSTSLSRLEDLLQDSMDEKEQLIQSKSSVASLVGRSKTIVQLKPRNPDHVLKSTLSVKAICDYRQIEITICKNDECVLEDNSQRTKWKVISPTGNEAMVPSVCFLIPPPNKEAIEMASRVEQSYQKVMALWHQLHINTKSLISWNYLRKDLDTVQTWSLEKLRSLAPGECHQVMKNLQAHYEDFLQDSHDSALFSVADRLRIEEEVEACKAHFQHLMKSLENEDKEETLAKVYISELKNIRLLLEECEQRLLKQIQSPASSKTDRDARQDITLRIAEQEHTQEDLQHLRSDLDAISMKCNVFLQQSPSGSSATTLRSELNLMVEKMDHVYGLSTVYLNKLKTIDVIVRSMQDAELLVKGYEIKLSQEEAVPADLSALESHRTTLQHWLSDVKDKNSVFSVLDEEITKAKKVAEQLRHPASEPNLDLERYQEKGSQLQERWHRVIAQLETRQSEVESIQEVLRDYRACHGTLIKWIEETTAQQEMMKPGQAEDSRVLSEQLSQQTELFAEIERNQTKLDQCQKFSQQYSTIVKDYELQLMTYKAFVESQQKSPGKRRRMISSSDAITQEFMDLRTRYTALVTLTTQHVKYISDALRRLEEEEKVVEEEKQEHVEKVKDLLGWVSTLARNTQGTTTSSHTSASADIEKAILEQQVLAEELTTKKEQVSEAIKTSQIFLAKHGHKLSEGEKEQISEQLRVLNKTYHDLCDGSANQLQQLQSELAQQTEQKGCRAVAGVIDLGTVEIFPIFRAMQKGLIDQDTGLVLLESQIIMSGLIDPENSEKLSLEEGLTRNFINLPIYQQLLGLRDSLSLVSRLTGTLGSLSVVEAIEKKIISERLGLKVLEVHLATGGFSLPPSENCINLEEAFHQGFIASSLHSELQSHLRSSKNLIDPNTAEKVGLLDLMQRCIIHQESGLKLLPVKQLAGGMVSLKSGRKVSIFRAVQEGLIDRQVTVRLLEAQLFAGGIVDPRTGHRLTVEEAVRHNLIDQDMACAILIRQLQTGGIIDTVTGDRMTIDEAVTNNLVAAKIALVILESLWSFMGLLLPESGEILPITDALEQGIVSTELAHKILHNRQQIEALFLPTLTEIWSWEKATESGILDKDLVNNLRSVCIPDMMPHIQLADSAEQSKVGFAAGKPPVSGPREEGSSHGEKLLFQLMTHSYIHAHTGQRLLLLDQELVEMLTSRDDCQVILPEVFEIQHQRLNTSEALQELYTGTISQISSAKHPRKPCESQFLSQNKDYPSQENCTEAKGERSVVGIECSPAESPERELFLKEQEAIIENVGSLKVINKVKLKLQRPLLGSRKEEQAETLREENISGDPLLVECPEESEGKDLSTEKSKCQTPTKCSFTCHKEQVKTIKDIPSETGTSLIKSQNQMSQFQVDTSVGLRSEFKSEHDMNVNSLEKELKEELLVKDGHKQSQEGQSVADGQTVALEKTDTEDNADEPALLHSSPFEDATLSTLSAQLQDGGIFNEETGQKLLLNEAIAQGLVSSHTAVKLMGKLNMFRGFFDSQTCESLTTEEVIDEGLMDEKLLYNVLMSDKAISGILDPRTHSLCSVKEAVAAGLLDKETATRILEGQVITGGIVDLKRGKKLSVTLASNLGLVDTADQTELINLEKATKGRGAEKAVKERLIELQMETAGLMDPESKAPLTVLQSIDRGILEREAAVYLLTKQVLDGGIIHHISGLRLSVDNAFKHGLIGEDMARQLRKVENFIHYQFFHPQTKEALSFSEAIKLDLVSPDLKREIQEIQDFSGNLGDFIYGQKLTLAKTNKEESLANKTELPSGVMHGVIDPENCTIIPYSELVKKCRIDTESGWRYLEVIPFSDIKDEAGNNVLTPPEAIQLGKVDFASALKVLEAQANTGGIIDMATGKRVTLASALEKKLLDENMARIIASHQMLSGGIIDIYSDQRVTLNDAVEKRLISPELAAMIQVDPLAEQGGTGVCELKGDFLRKELLSESSKTPRESYSKEKHEAVLQAGSLCAPEKAGIRGSNGEKAEKGRKISVEMEGQRQDEKASSDSKVSASILSPFGFEGESSYQVSVTHPCSESCDLKPREETRSCMKKCAVVERDKVVTQIKMVSHVKQSTSGLDAEEARERQGRMVSKEQGSHYETAGNLLSERSVRVDRRVRREMGGEQSVQMSREAAVLSEEESDQEVTIGDEPDSFVKSQSMKMIGNDKGKEAGIEKDISVVCKIEGFPSQMTSKDASLTNQDALPFYTEGETKTVNLCSILKPGEKLSQETASTVQKEPLSSEIPRPERLNSQESDEEPQISDVPHISKGDMAAQITTRQETTDVQDLYITSKSSETKDKIFPSKNYIEKLHQEIPMDPTRSHKLKEATISTLETEGISYLDSSDIKSLCEDSKADHKSCGHQKSKVTTTQAKKSLEVVDLLVRDTEEGSSEDRVGQRGPRVLASLLPEKLPTRTVQSENIRQHDAVIPAISEIREEMALSLPCSVVKVDGKIPKEKHKEILGDEQGPFMAIPSGKGIEGVNPEPCRATQNVFTRRLCLEHDEKLVSYLSLLRDIEMRTKQIQPLELNVAELQDLLGQAKELDRELKDLSTVVSQELECVDRIVISQPQEVPAQLLKALEKDAKNLQKSLDSVSDSWSSRFLHLQSAVEVKKATVLNRHKELQGKLQDLRAWVGRASLTLNSKGCDTETDADSLSHTLQPYKDMKQSMAERKSQLDALALDIQLFISEHPQDLSLQQNQEMLQFLSELQRSFQGLVEHTAAQKDVVQGHLQQVQQEVQVKTLQKQQDTCHKKLEDLCNWVGQAERALERHQGGSSSGKSSLLCAEPKVDLKDLQGDIQSHSTSFATAVKDIEGFLEENQTKLSPQELTALREKLHQAKEQYEVLQERTRVAQKELEEAVTSALQQETEKSKAATELAENKRKIDALLDWVGLLWGHLRESHRLAFQEWSSSLELAMEKQTLAATDGHVDVNQVPETLDRQYELMKARHQELLSQQQNFIVATQSVQSFLDQHSHNLTPEERQKLQEKLGELKEQYAASLARSEAELKQTQALRDELQKFLQDHKEFENWLQQSENELDSMHKGGSSPEALNSLLKRQGSFSEDVISHKGDLRFVTISGQKVLETENNFEEGQEPSATRNLVNEKLKDATERYTTLHSKCIRLGSHLSMLLGQYQQFQSSADSLQAWVLTCEASVGKLLSDTVASDPGVLQQQLATTKQLQEELAEHQVPVEKLQKAAHDLLDIEGEPALDCRPIQETTDSISSRFQNLSCSLDERSALLQKAIAQSQSVQESMESLLQSIREVEQNLERDQVASLSSGVIQEALANNMKLKQDIARQKSSLEATHDMVTRFMETADSNSASVLQGKLAELSQRFQQLQLQQQEKESNLKKLLPQAEMFEQLSNKLQQFMENKSRLLASGNQPDQDIAHFSQQIQELTLAMEDQKENLDTLEHLVTTLGSCGFALDLSQHQDKIQNLKKDFTELQKTVQEREKDASTCQEQLDEFRKLIRTFQKWLKETEGNVPPAKTFVSAKELEKQIEHLKDLISDWESKGALLGEINAKGTALESLIMDITAPDSQAKTGSILPPVGSSVGSVNGYHTCKDLTEIQCDMFDVNSKYEKLWEVLRERQESLQTVFSRMEEVQKEASSVLQWLESKEEVLKAMDATLSPTKTETVKAQAESNKAFLAELEQNSPKIQKVKEALAGLLKTYPNSQEAENWKKMQEDLNSRWEKATEVTVARQKQLEESASHLACFQAAESQLRPWLMEKELMMGVLGPLSIDPNMLKQQVQFMLKEFEARRQQHEQLNEAAQGILTGPGDMSPSASQVHKDLQSISQKWVELTDKLNSRSSQIDQAIVKSTQYQDLLQDLSEKVKAIGQRLSGQSAISTQPEAVKQQLEETSEIRSDLGQLDNEIKEAQTLCQELSLLIGEQYLKDELKKRLETVALPLQGLEDLAADRMNRLQAALASTQQFQQMFDELRTWLDEKQSQQAKNCPISAKLERLQCQLQENEEFQKNLNQHSGSYEVIVAEGEALLLSVPPGEEKKTLQNQLVELRSHWEDLSKKTANRQSRLKDCMQKAQKYQGHVEDLVPWIDECKSKMSELQVTLDPVQLESSLLRSKAMLNEAEKRRSLLEILNSAADILINSSEIDEDEIRDEKAGLNQNMDAITEELQAKTSSLEEMTQRLKEFQESFKNIEKKVEGAKHQLEIFDALGSQACSNKNLEKLKAQQEVLQALEPQVDYLRNFTQGLVEDAPDGSDASPLVHQAEVAQQEFLEVKQRVSSSCLTMENKLEGIGQFHCRVREMFSQLADLDDELDGMGAIGRDTDSLQSQIEDVRLFLNKIQALRFDIEDSEAECRKMLEEEGTLDLLGLKRELEALNKQCGKLTERGKVRQEQLELTLGRVEDFYRKLKALNDAATAAEEGEALQWIVGTEVDVINQQLADFKLFQKDQVDPLQVKLQQVNGLGQGLIQSAGKNCDVQGLEHDMDEINTRWNTLNKKVAQRIAQLQEALLHCGKFQDALEPLLSWLTDTEELIANQKPPSAEYKVVKAQIQEQKLLQRLLDDRKATVDMLQAEGGRIAQSAELADREKITGQLESLERRWTDLLSKAAARQKQLEDILVLAKQFHETAEPISDFLSVTANKLANSEPVGTQTAKIHQQIIRHKALNEEIINRKKNVDQAIKNGQALLKQTTGEEVLLIQEKLDGIKTRYADITLTSSKALRTLEQARQLATKFHSTYEELTGWLREAEEELAASGGQSPTGEQIPQFQQRQKELKKEVMEHRLVLDTVNEVSHALLELVPWRAREGLDKLVSDANEQYKLISDTVGQRVDEIDAAIQRSQQYEQAADAELAWVAETKRKLMALGPIRLEQDQTTAQLQVQKAFSIDIIRHKDSMDELFSHRGEIFSTCGEEQKAVLQEKTECLIQQYEAVSLLNSERYARLERAQVLVNQFWETYEELSPWAEETLALIAQLPPPAVDHEQLRQQQEEMRQLRESIAEHKPHIDKILKIGPQLKELNPEEGKMVEEKYQKAENMYAQIKDEVRQRALALDEAVSQSAQFHDKIEPMLETLENLSSRLRMPPLIPAEVDKIRECISDNKSATVELEKLQPSFEALKRRGEELIGRSQGADKDLAAKEIQDKLDQMVFFWEDIKARSEEREIKFLDVLELAEKFWYDMAALLTTIKDTQDIVHDLESPGIDPSIIKQQVEAAETIKEETDGLHEELEFIRILGADLIFACGETEKPEVKKSIDEMNNAWENLNKTWKERLEKLEDAMQAAVQYQDTLQAMFDWLDNTVIKLCTMPPVGTDLNTVKDQLNEMKEFKVEVYQQQIEMEKLNHQGELMLKKATDETDRDIIREPLTELKHLWENLGEKIAHRQHKLEGALLALGQFQHALEELMSWLTHTEELLDAQRPISGDPKVIEVELAKHHVLKNDVLAHQATVATVNKAGSELLESSAGDDASSLRSRLETMNQCWESVLQKTEEREQQLQSTLQQAQGFHSEIEDFLLELNRMENQLSASKPTGGLPETAREQLDTHMELHSQLRAKEEIYNQLLDKGRLMLLSRGDSGSGSKTEQSVALLEQKWHAVSSKVEERKSKLEEALSLATEFQNSLQEFINWLTLAEQSLNIASPPSLILNTVLSQIEEHKVFANEVNDHRDQIIELDQTGNQLKFLSQKQDVVLIKNLLVSVQSRWEKVVQRSIERGRSLDDARKRAKQFHEAWKKLIDWLEDAESHLDSELEISNDPDKIKLQLSKHKEFQKTLGGKQPVYDTTIRTGRALKEKTLLAGDTQKLDNLLGEVRDKWDTVCGKSVERQHKLEEALLFSGQFMDALQALVDWLYKVEPQLAEDQPVHGDLDLVMNLMDAHKVFQKELGKRTGTVQVLKRSGRELIEGSRDDTTWVKGQLQELSTRWDTVCKLSVSKQSRLEQALKQAEEFRDTVHMLLEWLSEAEQTLRFRGALPDDTEALQSLIDTHKEFMKKVEEKRVDVNTAVAMGEAILAVCHPDCITTIKHWITIIRARFEEVLTWAKQHQQRLETALSELVANAELLEELLAWIQWAETTLIQRDQEPIPQNIDRVKALITEHQSFMEEMTRKQPDVDRVTKTYKRKSVEPTHAPFMEKSRSGSRKSLNQPTPPPMPILSQSEAKNPRINQLSARWQQVWLLALERQRKLNDALDRLEELCPELKEFANFDFDVWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILASKFPTTKLEMTAVADIFDRDGDGYIDYYEFVAALHPNKDAYRPTTDADKIEDEVTRQVAQCKCAKRFQVEQIGENKYRFFLGNQFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRARGRTNIELREKFILPEGASQGMTPFRSRGRRSKPSSRAASPTRSSSSASQSNHSCTSMPSSPATPASGTKVISSSGSKLKRPTPAFHSSRTSLAGDTSNSSSPASTGAKANRADPKKSASRPGSRAGSRAGSRASSRRGSDASDFDLLETQSACSDTSESSAAGGQGSSRRGLTKPSKIPTMSKKTTTASPRTPGPKR 2B68195989DE15C7 rac1_mouse Ras-related C3 botulinum toxin substrate 1 Rac1 p21-Rac1 protein protein mouse Mus musculus Mouse MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL DIP protein P63001 original sequence version: 125 ACEDF83A45E5EA67 mare1_mouse Microtubule-associated protein RP/EB family member 1 Mapre1 APC-binding protein EB1 End-binding protein 1 protein protein mouse Mus musculus Mouse MAVNVYSTSVTSDNLSRHDMLAWINESLQLNLTKIEQLCSGAAYCQFMDMLFPGSIALKKVKFQAKLEHEYIQNFKILQAGFKRMGVDKIIPVDKLVKGKFQDNFEFVQWFKKFFDANYDGKEYDPVAARQGQETAVAPSLVAPALSKPKKPLGSSTAAPQRPIATQRTTAAPKAGPGMVRKNPGVGNGDDEAAELMQQVKVLKLTVEDLEKERDFYFGKLRNIELICQENEGENDPVLQRIVDILYATDEGFVIPDEGGPQEEQEEY DIP protein Q61166 original sequence version: 101 8FE3AFB7A5AE7762 rhoa_mouse Transforming protein RhoA Rhoa Arha Arha2 protein protein mouse Mus musculus Mouse MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLIL DIP protein Q9QUI0 original sequence version: 76 DIP protein Q9QUI0 original sequence version: 77 DIP protein Q9QUI0 original sequence version: 100 C4C8BDC31FF858BC cdc42_mouse Cell division control protein 42 homolog Cdc42 G25K GTP-binding protein protein protein mouse Mus musculus Mouse MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQKGLKNVFDEAILAALEPPEPKKSRRCVLL DIP protein P60766 original sequence version: 61 51A437E22A4D8FFF rtkn_human Rhotekin RTKN1 RTKN protein protein human Homo sapiens Human MFSRNHRSRVTVARGSALEMEFKRGRFRLSLFSDLPEDTELQRKLDHEIRMREGACKLLAACSQREQALEATKSLLVCNSRILSYMGELQRRKEAQVLGKTSRRPSDSGPPAERSPCRGRVCISDLRIPLMWKDTEYFKNKGDLHRWAVFLLLQLGEHIQDTEMILVDRTLTDISFQSNVLFAEAGPDFELRLELYGACVEEEGALTGGPKRLATKLSSSLGRSSGRRVRASLDSAGGSGSSPILLPTPVVGGPRYHLLAHTTLTLAAVQDGFRTHDLTLASHEENPAWLPLYGSVCCRLAAQPLCMTQPTASGTLRVQQAGEMQNWAQVHGVLKGTNLFCYRQPEDADTGEEPLLTIAVNKETRVRAGELDQALGRPFTLSISNQYGDDEVTHTLQTESREALQSWMEALWQLFFDMSQWKQCCDEIMKIETPAPRKPPQALAKQGSLYHEMAIEPLDDIAAVTDILTQREGARLETPPPWLAMFTDQPALPNPCSPASVAPAPDWTHPLPWGRPRTFSLDAVPPDHSPRARSVAPLPPQRSPRTRGLCSKGQPRTWLQSPV DIP protein Q9BST9 original sequence version: 112 B3C64CB737E88501 pak1_human Serine/threonine-protein kinase PAK 1 PAK1 p21-activated kinase 1 p65-PAK Alpha-PAK protein protein human Homo sapiens Human MSNNGLDIQDKPPAPPMRNTSTMIGAGSKDAGTLNHGSKPLPPNPEEKKKKDRFYRSILPGDKTNKKKEKERPEISLPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKSEQKKNPQAVLDVLEFYNSKKTSNSQKYMSFTDKSAEDYNSSNALNVKAVSETPAVPPVSEDEDDDDDDATPPPVIAPRPEHTKSVYTRSVIEPLPVTPTRDVATSPISPTENNTTPPDALTRNTEKQKKKPKMSDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAMDVATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRCLEMDVEKRGSAKELLQHQFLKIAKPLSSLTPLIAAAKEATKNNH DIP protein Q13153 original sequence version: 161 DIP protein Q13153 original sequence version: 122 DIP protein Q13153 original sequence version: 134 DIP protein Q13153 original sequence version: 165 1A95CD5F2195CD7B rtkn-rhoa-1 1 11 unspecified role unspecified role prey prey mouse-epithelium Mus musculus epithelium Epithelium [epithelial cells] 13 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association directly incubated with GST or GST-PBD (PAK p21 binding domain) or GST-RBD (Rhotekin Rho binding domain) proteins bound to glutathione-coupled Sepharose beads to selectively pull down active Rac/Cdc42 or Rho. Fig. 3E Rhotekin binds to GTP-bound, and not GDP-bound, RhoA. pak1-rac1-1 1 9 unspecified role unspecified role prey prey mouse-epithelium Mus musculus epithelium Epithelium [epithelial cells] 14 unspecified role unspecified role bait bait pak box region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association directly incubated with GST or GST-PBD (PAK p21 binding domain) or GST-RBD (Rhotekin Rho binding domain) proteins bound to glutathione-coupled Sepharose beads to selectively pull down active Rac/Cdc42 or Rho. Fig. 3F pak1-cdc42-3 1 12 unspecified role unspecified role prey prey mouse-epithelium Mus musculus epithelium Epithelium [epithelial cells] 14 unspecified role unspecified role bait bait pak box region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association directly incubated with GST or GST-PBD (PAK p21 binding domain) or GST-RBD (Rhotekin Rho binding domain) proteins bound to glutathione-coupled Sepharose beads to selectively pull down active Rac/Cdc42 or Rho. Fig. 3F macf1-mare1-1 2 6 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey association association As shown in Figure4C, keratinocyte ACF7 specifically coimmunoprecipitated with +tip proteins EB1, Clasp1, and Clasp2, Fig. 4C macf1-macf1_mouse_de 4 8 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range range range certain certain sequence position certain 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey association association Pull-down assays showed that both full-length ACF7 and ACF7-NC (81 kDa) associated with F-actin, MTs, and +tip proteins (Figures 5B and 5C). Fig. 5C The composition of the Macf1 construct has been inferred from the author's free test description, the diagram in Supp. Fig. 7 and information in the UniProtKB entry. None of the isoforms described in UniProtKB match the length of the protein described in this paper (5380 amino acids). macf1-atp-1 3 7 enzyme target enzyme target substrate substrate neutral component neutral component 8 enzyme enzyme neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) atpase reaction atpase reaction Fig. 6C, 6E, 6F Autoradiography and quantification revealed significant ATP hydrolysis for full-length ACF7 in comparison to ACF7-NC or GST (Figure6C) F-actin