IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Solution structure of the integral human membrane protein VDAC-1 in detergent micelles. Solution structure of the integral human membrane protein VDAC-1 in detergent micelles. Science (0036-8075) 2008 mimix curation Hiller S., Garces R.G., Malia T.J., Orekhov V.Y., Colombini M., Wagner G. Apoptosis - Interactions involving proteins with a function related to apoptosis NMR analysis was performed in solution, placing VCAP-1 into lauryldimethylamine oxide (LDAO) detergent micelles. gerhard_wagner@hms.harvard.edu 21-Dec-2011: Contacted by JYOTI. in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Hiller S., Garces R.G., Malia T.J., Orekhov V.Y., Colombini M., Wagner G. Apoptosis - Interactions involving proteins with a function related to apoptosis NMR analysis was performed in solution, placing VCAP-1 into lauryldimethylamine oxide (LDAO) detergent micelles. gerhard_wagner@hms.harvard.edu Accepted 2011-OCT-24 by ORCHARD Science (0036-8075) 2008 mimix curation cholesterol cholesterol small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) nadh Nicotinamide adenine dinucleotide, reduced form small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) vdac1_human Voltage-dependent anion-selective channel protein 1 VDAC1 VDAC Outer mitochondrial membrane protein porin 1 Plasmalemmal porin Porin 31HL Porin 31HM protein protein human Homo sapiens Human MAVPPTYADLGKSARDVFTKGYGFGLIKLDLKTKSENGLEFTSSGSANTETTKVTGSLETKYRWTEYGLTFTEKWNTDNTLGTEITVEDQLARGLKLTFDSSFSPNTGKKNAKIKTGYKREHINLGCDMDFDIAGPSIRGALVLGYEGWLAGYQMNFETAKSRVTQSNFAVGYKTDEFQLHTNVNDGTEFGGSIYQKVNKKLETAVNLAWTAGNSNTRFGIAAKYQIDPDACFSAKVNNSSLIGLGYTQTLKPGIKLTLSALLDGKNVNAGGHKLGLGLEFQA DIP protein P21796 original sequence version: 103 DIP protein P21796 original sequence version: 123 DIP protein P21796 original sequence version: 125 89BA3378B04020D5 cholesterol-vdac1 1 4 unspecified role unspecified role neutral component neutral component purified purified 15n isotope labeling 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cholesterol binding sites sufficient to bind sufficient binding region sufficient to bind range range range range range range range range The mapping of these interaction sites corresponds to strands 7, 8 and 11, depicting two distinct binding areas, one in strands 7 and 8 and another in strand 11 (fig. 4a). his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 2h isotope labeling 2h label 2h label deuterium D2 2H2 2H2 deuterium D2 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 2 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 4a, S12 nadh-vdac1 1 3 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 4 unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range nadh binding site sufficient to bind sufficient binding region sufficient to bind range range range range The mapping of this interaction site corresponds to strands 17 and 18, specifically affecting residues 242-244; 261 and 263-264 (fig. S13). 15n isotope labeling 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 2h isotope labeling 2h label 2h label deuterium D2 2H2 2H2 deuterium D2 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 4b, S13c