IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. Nat. Cell Biol. (1465-7392) 2008 imex curation Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu 21-Dec-2011: Contacted by JYOTI. Human fetal brain cDNA Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu Accepted 2011-OCT-12 by ORCHARD 21-Dec-2011: Contacted by JYOTI. Human fetal brain cDNA Nat. Cell Biol. (1465-7392) 2008 Only protein-protein interactions imex curation Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. Nat. Cell Biol. (1465-7392) 2008 imex curation Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu 21-Dec-2011: Contacted by JYOTI. Human fetal brain cDNA Only protein-protein interactions rat-brain-cortex Rattus norvegicus brain cortex brain_cortex Brain cortex [Cerebral cortex; Cerebrum cortex; Cortex; Pallium] confocal microscopy confocal microscopy immunostaining immunostaining Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu Accepted 2011-OCT-12 by ORCHARD 21-Dec-2011: Contacted by JYOTI. Human fetal brain cDNA Nat. Cell Biol. (1465-7392) 2008 Only protein-protein interactions imex curation Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. Nat. Cell Biol. (1465-7392) 2008 imex curation Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu 21-Dec-2011: Contacted by JYOTI. Human fetal brain cDNA Only protein-protein interactions rat-brain-cortex Rattus norvegicus brain cortex brain_cortex Brain cortex [Cerebral cortex; Cerebrum cortex; Cortex; Pallium] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu Accepted 2011-OCT-12 by ORCHARD 21-Dec-2011: Contacted by JYOTI. Human fetal brain cDNA Nat. Cell Biol. (1465-7392) 2008 Only protein-protein interactions imex curation Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. Nat. Cell Biol. (1465-7392) 2008 imex curation Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu 21-Dec-2011: Contacted by JYOTI. Human fetal brain cDNA Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu Accepted 2011-OCT-12 by ORCHARD 21-Dec-2011: Contacted by JYOTI. Human fetal brain cDNA Nat. Cell Biol. (1465-7392) 2008 Only protein-protein interactions imex curation Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. Nat. Cell Biol. (1465-7392) 2008 imex curation Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu 21-Dec-2011: Contacted by JYOTI. Human fetal brain cDNA Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu Accepted 2011-OCT-12 by ORCHARD 21-Dec-2011: Contacted by JYOTI. Human fetal brain cDNA Nat. Cell Biol. (1465-7392) 2008 Only protein-protein interactions imex curation Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. Nat. Cell Biol. (1465-7392) 2008 imex curation Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu 21-Dec-2011: Contacted by JYOTI. Human fetal brain cDNA Only protein-protein interactions rat-brain-hippocampus Hippocampus of rat brain rat-brain-hippocampus Hippocampus of rat brain confocal microscopy confocal microscopy immunostaining immunostaining Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu Accepted 2011-OCT-12 by ORCHARD 21-Dec-2011: Contacted by JYOTI. Human fetal brain cDNA Nat. Cell Biol. (1465-7392) 2008 Only protein-protein interactions imex curation Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. Nat. Cell Biol. (1465-7392) 2008 imex curation Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu 21-Dec-2011: Contacted by JYOTI. Human fetal brain cDNA Only protein-protein interactions rat-brain-hippocampus Hippocampus of rat brain rat-brain-hippocampus Hippocampus of rat brain anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu Accepted 2011-OCT-12 by ORCHARD 21-Dec-2011: Contacted by JYOTI. Human fetal brain cDNA Nat. Cell Biol. (1465-7392) 2008 Only protein-protein interactions imex curation Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. Nat. Cell Biol. (1465-7392) 2008 imex curation Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu 21-Dec-2011: Contacted by JYOTI. Human fetal brain cDNA Only protein-protein interactions mouse-brain Mus musculus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu Accepted 2011-OCT-12 by ORCHARD 21-Dec-2011: Contacted by JYOTI. Human fetal brain cDNA Nat. Cell Biol. (1465-7392) 2008 Only protein-protein interactions imex curation Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. Nat. Cell Biol. (1465-7392) 2008 imex curation Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu 21-Dec-2011: Contacted by JYOTI. Human fetal brain cDNA Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu Accepted 2011-OCT-12 by ORCHARD Human fetal brain cDNA Nat. Cell Biol. (1465-7392) 2008 Only protein-protein interactions imex curation Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. Nat. Cell Biol. (1465-7392) 2008 imex curation Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu 21-Dec-2011: Contacted by JYOTI. Human fetal brain cDNA Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu Accepted 2011-OCT-12 by ORCHARD 21-Dec-2011: Contacted by JYOTI. Human fetal brain cDNA Nat. Cell Biol. (1465-7392) 2008 Only protein-protein interactions imex curation dcc_rat Netrin receptor DCC Dcc Tumor suppressor protein DCC protein protein rat Rattus norvegicus (Rat) Rat MENSLGCVWVPKLAFVLFGASLLSAHLQVTGFQIKPFTSLHFVSEPSDAVTMRGGNVLLNCSAESDRGVPVIKWKKDGLILALGMDDRKQQLPNGSLLIQNILHSRHHKPDEGLYQCEASLGDSGSIISRTAKVMVAGPLRFLSQTESITAFMGDTVLLKCEVIGDPMPTIHWQKNQQDLNPIPGDSRVVVLPSGALQISRLQPGDSGVYRCSARNPASTRTGNEAEVRILSDPGLHRQLYFLQRPSNVIAIEGKDAVLECCVSGYPPPSFTWLRGEEVIQLRSKKYSLLGGSNLLISNVTDDDSGTYTCVVTYKNENISASAELTVLVPPWFLNHPSNLYAYESMDIEFECAVSGKPVPTVNWMKNGDVVIPSDYFQIVGGSNLRILGVVKSDEGFYQCVAENEAGNAQSSAQLIVPKPAIPSSSILPSAPRDVVPVLVSSRFVRLSWRPPAEAKGNIQTFTVFFSREGDNRERALNTTQPGSLQLTVGNLKPEAMYTFRVVAYNEWGPGESSQPIKVATQPELQVPGPVENLHAVSASPTSILITWEPPAYANGPVQGYRLFCTEVSTGKEQNIEVDGLSYKLEGLKKFTEYTLRFLAYNRYGPGVSTDDITVVTLSDVPSAPPQNVSLEVVNSRSIKVSWLPPPSGTQNGFITGYKIRHRKTTRRGEMETLEPNNLWYLFTGLEKGSQYSFQVSAMTVNGTGPPSNWYTAETPENDLDESQVPDQPSSLHVRPQTNCIIMSWTPPLNPNIVVRGYIIGYGVGSPYAETVRVDSKQRYYSIERLESSSHYVISLKAFNNAGEGVPLYESATTRSITDPTDPVDYYPLLDDFPTSGPDVSTPMLPPVGVQAVALTHEAVRVSWADNSVPKNQKTSDVRLYTVRWRTSFSASAKYKSEDTTSLSYTATGLKPNTMYEFSVMVTKNRRSSTWSMTAHATTYEAAPTSAPKDLTVITREGKPRAVIVSWQPPLEANGKITAYILFYTLDKNIPIDDWIMETISGDRLTHQIMDLSLDTMYYFRIQARNAKGVGPLSDPILFRTLKVEHPDKMANDQGRHGDGGYWPVDTNLIDRSTLNEPPIGQMHPPHGSVTPQKNSNLLVITVVTVGVLTVLVVVIVAVICTRRSSAQQRKKRATHSASKRKGSQKDLRPPDLWIHHEEMEMKNIEKPAGTDPAGRGSPIQSCQDLTPVSHSQSESQMGSKSASHSGQDTEEAGSSMSTLERSLAARRATRTKLMIPMEAQSNNPAVVSAIPVPTLESAQYPGILPSPTCGYPHPQFTLRPVPFPTLSVDRGFGAGRTVSEGPTAQQQPMLPPAQPEHPSSEEAPSRTIPTACVRPTHPLRSFANPLLPPPMSAIEPKVPYTPLLSQPGPTLPKTHVKTASLGLAGKARSPLLPVSVPTAPEVSEESHKPTEDPASVYEQDDLSEQMASLEGLMKQLNAITGSAF DIP protein Q63155 original sequence version: 91 DIP protein Q63155 original sequence version: 76 084F625954481988 agap2_rat Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 Agap2 Centg1 Centaurin-gamma-1 Phosphatidylinositol 3-kinase enhancer protein protein rat Rattus norvegicus (Rat) Rat MSRGAGALQRRTTTYLISLTLVKLESVPPPPPSPSAAAVGAPGARGSEPRDPGSPRGAEEPGKKRHERLFHRQDALWISTSSAGAGGAEPPALSPAPASPARPVSPAPGRRLSLWAAPPGPPLSGGLSPDSKPGGAPSSSRRPLLSSPSWGGPEPEGRTGGGVPGSSSPHPGTGSRRLKVAPPPPAPKPFKTVTTSGAKAGGGKGAGSRLSWPESEGKPRVKGSKSTAGTGASAVAAGGGGSAAVTTSGGVGAGAGARGKLSPRKGKSKTLDNSDLHPGPSAGSPPLTVPAIPVPATSVTAASTQPLGPAPPITLEPPAPGLKRGREGGRASTRDRKMLKFISGIFTKSTGGPPGPGPLPGPQGLSSSSGSRELLGAELRASPKAVVNSQEWTLSRSIPELRLGVLGDVRSGKSSLIHRFLTGSYQVLEKPESEQYKKEMLVDGQTHLVLIREEAGAPDAKFSGWADAVIFVFSLEDESSFQAVSHLHGQLISLRGEGRGGLALALVGTQDRISASSPRVVGDARARALCTDMKRCSYYETCATYGLNVDRVFQEVAQKVVTLRKQQQLLAACKSLPSSPSHSAASTPVAGQASNGGHTSDYSSSLPSSPNVGHRELRAEAAAVAGLSTPGSLHRAAKRRTSLFANRRGSDSEKRSLDSRGETTGSGRAIPIKQSFLLKRSGNSLNKEWKKKYVTLSSNGFLLYHPSINDYIHSTHGKEMDLLRTTVKVPGKRPPRAISAFGPSASINGLVKDMSTVQMGEGPEASTPMPSPSPSPSSLQLPTDQTSKHLLKPDRNLARALSTDCTPSGDLSPLSREPPPSPMVKKQRRKKLSTPSKTEGSAVQAEAKRKMWKLKSFGSLRNIYKAEENFEFLIVSSTGQTWHFEAASFEERDAWVQAIESQILASLQCCESSKVKLRTDSQSEAVAIQAIRNAKGNSTCVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPRELTLVLTAIGNDTANRVWESDTRGRAKPTRDSSREERESWIRAKYEQLLFLAPLGTTEEPLGRQLWAAVEAQDVAAVLLLLAHARHGPLDTSVEDPQLRSPLHLAAELAHVVITQLLLWYGADVAARDAQGRTALFYARQAGSQLCADILLQHGCPGEGGSTATTPSAATTPSITATPSPRRRSSAASLGRVDTTIALV B9C8E987D2989378 unc5b_rat Netrin receptor UNC5B Unc5b Unc5h2 Protein unc-5 homolog 2 Protein unc-5 homolog B protein protein rat Rattus norvegicus (Rat) Rat MRARSGARGALLLALLLCWDPTPSLAGIDSGGQALPDSFPSAPAEQLPHFLLEPEDAYIVKNKPVELHCRAFPATQIYFKCNGEWVSQKGHVTQESLDEATGLRIREVQIEVSRQQVEELFGLEDYWCQCVAWSSSGTTKSRRAYIRIAYLRKNFDQEPLAKEVPLDHEVLLQCRPPEGVPVAEVEWLKNEDVIDPAQDTNFLLTIDHNLIIRQARLSDTANYTCVAKNIVAKRRSTTATVIVYVNGGWSSWAEWSPCSNRCGRGWQKRTRTCTNPAPLNGGAFCEGQACQKTACTTVCPVDGAWTEWSKWSACSTECAHWRSRECMAPPPQNGGRDCSGTLLDSKNCTDGLCVLNQRTLNDPKSRPLEPSGDVALYAGLVVAVFVVLAVLMAVGVIVYRRNCRDFDTDITDSSAALTGGFHPVNFKTARPSNPQLLHPSAPPDLTASAGIYRGPVYALQDSADKIPMTNSPLLDPLPSLKIKVYDSSTIGSGAGLADGADLLGVLPPGTYPGDFSRDTHFLHLRSASLGSQHLLGLPRDPSSSVSGTFGCLGGRLTIPGTGVSLLVPNGAIPQGKFYDLYLRINKTESTLPLSEGSQTVLSPSVTCGPTGLLLCRPVVLTVPHCAEVIAGDWIFQLKTQAHQGHWEEVVTLDEETLNTPCYCQLEAKSCHILLDQLGTYVFTGESYSRSAVKRLQLAIFAPALCTSLEYSLRVYCLEDTPAALKEVLELERTLGGYLVEEPKTLLFKDSYHNLRLSLHDIPHAHWRSKLLAKYQEIPFYHVWNGSQKALHCTFTLERHSLASTEFTCKVCVRQVEGEGQIFQLHTTLAETPAGSLDALCSAPGNAATTQLGPYAFKIPLSIRQKICNSLDAPNSRGNDWRLLAQKLSMDRYLNYFATKASPTGVILDLWEARQQDDGDLNSLASALEEMGKSEMLVAMTTDGDC DIP protein O08722 original sequence version: 100 6E9C2A262E560B9B dcc_mouse Netrin receptor DCC Dcc Tumor suppressor protein DCC protein protein mouse Mus musculus Mouse MENSLGCVWVPKLAFVLFGASLLSAHLQVTGFQIKPFTSLHFVSEPSDAVTMRGGNVLLNCSAESDRGVPVIKWKKDGLILALGMDDRKQQLPNGSLLIQNILHSRHHKPDEGLYQCEASLADSGSIISRTAKVTVAGPLRFLSQTESITAFMGDTVLLKCEVIGEPMPTIHWQKNQQDLNPLPGDSRVVVLPSGALQISRLQPGDSGVYRCSARNPASIRTGNEAEVRILSDPGLHRQLYFLQRPSNVIAIEGKDAVLECCVSGYPPPSFTWLRGEEVIQLRSKKYSLLGGSNLLISNVTDDDSGTYTCVVTYKNENISASAELTVLVPPWFLNHPSNLYAYESMDIEFECAVSGKPVPTVNWMKNGDVVIPSDYFQIVGGSNLRILGVVKSDEGFYQCVAENEAGNAQSSAQLIVPKPAIPSSSILPSAPRDVLPVLVSSRFVRLSWRPPAEAKGNIQTFTVFFSREGDNRERALNTTQPGSLQLTVGNLKPEAMYTFRVVAYNEWGPGESSQPIKVATQPELQVPGPVENLHAVSTSPTSILITWEPPAYANGPVQGYRLFCTEVSTGKEQNIEVDGLSYKLEGLKKFTEYTLRFLAYNRYGPGVSTDDITVVTLSDVPSAPPQNISLEVVNSRSIKVSWLPPPSGTQNGFITGYKIRHRKTTRRGEMETLEPNNLWYLFTGLEKGSQYSFQVSAMTVNGTGPPSNWYTAETPENDLDESQVPDQPSSLHVRPQTNCIIMSWTPPLNPNIVVRGYIIGYGVGSPYAETVRVDSKQRYYSIERLESSSHYVISLKAFNNAGEGVPLYESATTRSITDPTDPVDYYPLLDDFPTSGPDVSTPMLPPVGVQAVALTHEAVRVSWADNSVPKNQKTSDVRLYTVRWRTSFSASAKYKSEDTTSLSYTATGLKPNTMYEFSVMVTKNRRSSTWSMTAHATTYEAAPTSAPKDLTVITREGKPRAVIVSWQPPLEANGKITAYILFYTLDKNIPIDDWIMETISGDRLTHQIMDLSLDTMYYFRIQARNVKGVGPLSDPILFRTLKVEHPDKMANDQGRHGDGGYWPVDTNLIDRSTLNEPPIGQMHPPHGSVTPQKNSNLLVITVVTVGVLTVLVVVIVAVICTRRSSAQQRKKRATHSVSKRKGSQKDLRPPDLWIHHEEMEMKNIEKPTGTDPAGRDSPIQSCQDLTPVSHSQSETQMGSKSASHSGQDTEDAGSSMSTLERSLAARRATRAKLMIPMEAQSSNPAVVSAIPVPTLESAQYPGILPSPTCGYPHPQFTLRPVPFPTLSVDRGFGAGRTQSVSEGPTTQQQPMLPPAQPEHPSSEEAPSRTIPTACVRPTHPLRSFANPLLPPPMSAIEPKVPYTPLLSQPGPTLPKTHVKTASLGLAGKARSPLLPVSVPTAPEVSEESHKPTEDPASVYEQDDLSEQMASLEGLMKQLNAITGSAF F905BE27C991EA42 unc5b_human Netrin receptor UNC5B UNC5B P53RDL1 UNC5H2 Protein unc-5 homolog 2 Protein unc-5 homolog B p53-regulated receptor for death and life protein 1 UNQ1883/PRO4326 protein protein human Homo sapiens Human MGARSGARGALLLALLLCWDPRLSQAGTDSGSEVLPDSFPSAPAEPLPYFLQEPQDAYIVKNKPVELRCRAFPATQIYFKCNGEWVSQNDHVTQEGLDEATGLRVREVQIEVSRQQVEELFGLEDYWCQCVAWSSAGTTKSRRAYVRIAYLRKNFDQEPLGKEVPLDHEVLLQCRPPEGVPVAEVEWLKNEDVIDPTQDTNFLLTIDHNLIIRQARLSDTANYTCVAKNIVAKRRSTTATVIVYVNGGWSSWAEWSPCSNRCGRGWQKRTRTCTNPAPLNGGAFCEGQAFQKTACTTICPVDGAWTEWSKWSACSTECAHWRSRECMAPPPQNGGRDCSGTLLDSKNCTDGLCMQNKKTLSDPNSHLLEASGDAALYAGLVVAIFVVVAILMAVGVVVYRRNCRDFDTDITDSSAALTGGFHPVNFKTARPSNPQLLHPSVPPDLTASAGIYRGPVYALQDSTDKIPMTNSPLLDPLPSLKVKVYSSSTTGSGPGLADGADLLGVLPPGTYPSDFARDTHFLHLRSASLGSQQLLGLPRDPGSSVSGTFGCLGGRLSIPGTGVSLLVPNGAIPQGKFYEMYLLINKAESTLPLSEGTQTVLSPSVTCGPTGLLLCRPVILTMPHCAEVSARDWIFQLKTQAHQGHWEEVVTLDEETLNTPCYCQLEPRACHILLDQLGTYVFTGESYSRSAVKRLQLAVFAPALCTSLEYSLRVYCLEDTPVALKEVLELERTLGGYLVEEPKPLMFKDSYHNLRLSLHDLPHAHWRSKLLAKYQEIPFYHIWSGSQKALHCTFTLERHSLASTELTCKICVRQVEGEGQIFQLHTTLAETPAGSLDTLCSAPGSTVTTQLGPYAFKIPLSIRQKICNSLDAPNSRGNDWRMLAQKLSMDRYLNYFATKASPTGVILDLWEALQQDDGDLNSLASALEEMGKSEMLVAVATDGDC DIP protein Q8IZJ1 original sequence version: 55 56064E335F323447 agap2_mouse Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 Agap2 Centg1 Centaurin-gamma-1 Kiaa0167 Phosphatidylinositol 3-kinase enhancer protein protein mouse Mus musculus Mouse MSRGAGALQRRTTTYLISLTLVKLESVPPPPPSPSAAAAGAPGARGSEPRDPGSPRGSEEPGKKRHERLFHRQDALWISTSSAGTGGAEPPALSPAPASPARPVSPAPGRRLSLWAAPPGPPLSGGLSPDSKPGGAPSSSRRPLLSSPSWGGPEPEGRTGGGVPGSSSPHPGTGSRRLKVAPPPPAPKPFKTVTTSGAKAGGGKGAGSRLSWPESEGKPRVKGSKSTAGTGASAAAAGGGGSAAVTTSGGVGAGAGTRGKLSPRKGKSKTLDNSDLHPGPSAGSPPLTVPAIPVPATSVTATSTQPLGPAPPITLEPPAPGLKRGREGGRASTRDRKMLKFISGIFTKSTGGPPGPGPLPGPQGLSSSSGSRELLGAELRTSPKAVVNSQEWTLSRSIPELRLGVLGDVRSGKSSLIHRFLTGSYQVLEKIESEQYKKEMLVDGQTHLVLIREEAGAPDAKFSGWADAVIFVFSLEDESSFQAVSRLHGQLSSLRGEGRGGLALALVGTQDRISASSPRVVGDARARALCTDMKRCSYYETCATYGLNVDRVFQEVAQKVVTLRKQQQLLAACKSLPSSPSHSAASTPVAGQASNGGHTSDYSSSLPSSPNVGHRELRAEAAAVAGLSTPGSLHRAAKRRTSLFANRRGSDAEKRSLDSRGETTGSGRAIPIKQSFLLKRSGNSLNKEWKKKYVTLSSNGFLLYHPSINDYIHSTHGKEMDLLRTTVKVPGKRPPRAISAFGPSASINGLVKDMSTVQMGEGPEASTPMPSPSPSPSSLQLPTDQTSKHLLKPDRNLARALSTDCTPSGDLSPLSREPPPSPMVKKQRRKKLSTPSKTEGSAVQAEAKRKMWKLKSFGSLRNIYKAEENFEFLIVSSTGQTWHFEAASFEERDAWVQAIESQILASLQCCESSKVKLRTDSQSEAVAIQAIRNAKGNSTCVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPRELTLVLTAIGNDTANRVWESDTRGRAKPTRDSSREERESWIRAKYEQLLFLAPLGTTEEPLGRQLWAAVEAQDVAAVLLLLAHARHGPLDTSVEDPQLRSPLHLAAELAHVVITQLLLWYGADVAARDAQGRTALFYARQAGSQLCADILLQHGCPGEGGSTATTPSAATTPSITATPSPRRRSSAASLGRVDTTIALV E9B4F3EDB66CBEC8 unc5b_mouse Netrin receptor UNC5B Unc5b Unc5h2 Protein unc-5 homolog 2 Protein unc-5 homolog B protein protein mouse Mus musculus Mouse MRARSGVRSALLLALLLCWDPTPSLAGVDSAGQVLPDSYPSAPAEQLPYFLLEPQDAYIVKNKPVELHCRAFPATQIYFKCNGEWVSQNDHVTQESLDEATGLRVREVQIEVSRQQVEELFGLEDYWCQCVAWSSSGTTKSRRAYIRIAYLRKNFDQEPLAKEVPLDHEVLLQCRPPEGVPVAEVEWLKNEDVIDPAQDTNFLLTIDHNLIIRQARLSDTANYTCVAKNIVAKRRSTTATVIVYVNGGWSSWAEWSPCSNRCGRGWQKRTRTCTNPAPLNGGAFCEGQAFQKTACTTVCPVDGAWTEWSKWSACSTECAHWRSRECMAPPPQNGGRDCSGTLLDSKNCTDGLCVLNQRTLNDPKSHPLETSGDVALYAGLVVAVFVVVAVLMAVGVIVYRRNCRDFDTDITDSSAALTGGFHPVNFKTARPNNPQLLHPSAPPDLTASAGIYRGPVYALQDSADKIPMTNSPLLDPLPSLKIKVYNSSTIGSGSGLADGADLLGVLPPGTYPGDFSRDTHFLHLRSASLGSQHLLGLPRDPSSSVSGTFGCLGGRLSLPGTGVSLLVPNGAIPQGKFYDLYLHINKAESTLPLSEGSQTVLSPSVTCGPTGLLLCRPVVLTVPHCAEVIAGDWIFQLKTQAHQGHWEEVVTLDEETLNTPCYCQLEAKSCHILLDQLGTYVFMGESYSRSAVKRLQLAIFAPALCTSLEYSLRVYCLEDTPVALKEVLELERTLGGYLVEEPKPLLFKDSYHNLRLSLHDIPHAHWRSKLLAKYQEIPFYHVWNGSQRALHCTFTLERHSLASTEFTCKVCVRQVEGEGQIFQLHTTLAETPAGSLDALCSAPGNAITTQLGPYAFKIPLSIRQKICSSLDAPNSRGNDWRLLAQKLSMDRYLNYFATKASPTGVILDLWEARQQDDGDLNSLASALEEMGKSEMLVAMATDGDC 80E896F0F0E06012 agap2-unc5b 9 15 unspecified role unspecified role prey prey c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role bait bait gtp-ase domain sufficient to bind sufficient binding region sufficient to bind range range range range 22 20 physical association physical association 1a agap2-unc5b-2 5 15 unspecified role unspecified role prey prey ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain c-terminal region without death domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain This region binds agap2 with less affinity than the full c-terminal fragment (568-945). 12 unspecified role unspecified role bait bait gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1b unc5b-agap2 5 12 unspecified role unspecified role prey prey gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 15 unspecified role unspecified role bait bait c-terminal region sufficient to bind sufficient binding region sufficient to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal region without death domain required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain This region binds agap2 with less affinity than the full c-terminal fragment (568-945). physical association physical association 1b agap2-unc5b-3 5 12 unspecified role unspecified role bait bait myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined gtp-ase domain required to bind necessary binding region required to bind mutation analysis mutation analysis range range range range 15 unspecified role unspecified role prey prey ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1b unc5b-agap2-1 1 15 unspecified role unspecified role bait bait ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role prey prey gtp-ase domain required to bind necessary binding region required to bind mutation analysis mutation analysis range range range range myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1b agap2-unc5b-4 3 12 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey physical association physical association 1c, S6, 2c This interaction is disrupted by tyrosine-kinase inhibitors genistein adn PP2 (fig. 2c). The presence of netrin-1 increases the interaction (fig. 2c). agap2-unc5b-5 7 13 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait physical association physical association 1c, S6 unc5b-agap2-3 7 13 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey physical association physical association 1c, S6 agap2-unc5b-7 2 12 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component colocalization colocalization 1d agap2-unc5b-8 6 13 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component colocalization colocalization 1d unc5b-agap2-4 5 12 unspecified role unspecified role prey prey Q8CGU4:p.[Lys413Ala;Ser414Asn] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain S N Wed May 02 16:49:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys413ala,ser414asn' Tue Jun 30 16:43:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys413Ala;Ser414Asn]' This mutation disrupts GTPase activity of agap2 as well as its interaction with unc5b. gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 15 unspecified role unspecified role bait bait ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2a The presence of purified netrin-1 is required for the interaction to happen. unc5b-agap2-5 5 15 unspecified role unspecified role bait bait ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role prey prey Q8CGU4:p.[Tyr1032Phe;Tyr1124Phe] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F Fri Jul 22 11:07:29 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr1124phe,tyr1032phe' Wed May 02 20:12:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr1032phe,tyr1124phe' Tue Jun 30 20:19:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr1032Phe;Tyr1124Phe]' Q8CGU4:p.Tyr1124Phe mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 15:47:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr1124phe' Tue Jun 30 19:05:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr1124Phe' myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q8CGU4:p.Tyr1032Phe mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 20:27:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr1032phe' Tue Jun 30 16:16:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr1032Phe' physical association physical association 2b, 3a The presence of purified netrin-1 is required for the interaction to happen. The interaction is inhibited by PP2 (selective inhibitor of Fyn, a tyrosine kinase that phosphorylates unc5b), genistein and compund 5 (both tyrosine kinase inhibitors). agap2-unc5b-9 5 12 unspecified role unspecified role bait bait Q8CGU4:p.Tyr1124Phe mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 20:30:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr1124phe' Tue Jun 30 15:38:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr1124Phe' myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q8CGU4:p.Tyr1032Phe mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 18:28:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr1032phe' Tue Jun 30 17:52:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr1032Phe' Q8CGU4:p.[Tyr1032Phe;Tyr1124Phe] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F Fri Jul 22 10:58:10 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr1124phe,tyr1032phe' Wed May 02 19:50:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr1032phe,tyr1124phe' Tue Jun 30 16:31:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr1032Phe;Tyr1124Phe]' 15 unspecified role unspecified role prey prey ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2b, S6, 3a, S1c The presence of purified netrin-1 is required for the interaction to happen (figs. 2b, S6 and 3a). In the absence of netrin-1, the presence of FynA is required (fig. S1c). The interaction is inhibited by PP2 (selective inhibitor of Fyn, a tyrosine kinase that phosphorylates unc5b), genistein and compund 5 (both tyrosine kinase inhibitors). unc5b-agap2-6 3 13 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey physical association physical association 1c, S6, 2c This interaction is disrupted by tyrosine-kinase inhibitors genistein adn PP2 (fig. 2c). The presence of netrin-1 increases the interaction (fig. 2c). unc5b-agap2-7 1 15 unspecified role unspecified role bait bait ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role prey prey myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association S1c The presence of FynA is required for the interaction to happen. agap2-unc5b-12 8 16 unspecified role unspecified role unspecified role unspecified role 17 unspecified role unspecified role unspecified role unspecified role physical association physical association 3b The presence of FynA is required for the interaction to happen. Inmunoprecipitation performed using anti-PY99, an anti-phospho tyrosine antibody. dcc-agap2 10 11 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role prey prey cell lysate cell lysate myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association S2a dcc-agap2-1 5 12 unspecified role unspecified role prey prey myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association S2b dcc-agap2-2 8 16 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait physical association physical association S2c unc5b-dcc 4 15 unspecified role unspecified role bait bait ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association S2d The presence of either netrin-1, Fyn or GFP-agap2 is required for the interaction to happen.