IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ SKAR links pre-mRNA splicing to mTOR/S6K1-mediated enhanced translation efficiency of spliced mRNAs. SKAR links pre-mRNA splicing to mTOR/S6K1-mediated enhanced translation efficiency of spliced mRNAs. Cell (0092-8674) 2008 imex curation Ma XM., Yoon SO., Richardson CJ., Julich K., Blenis J. Apoptosis - Interactions involving proteins with a function related to apoptosis I have not attempted to capture the RNA binding data as it appears to have been done with a heterogeneous mRNA population. Author used 293E cells - a sub-clone of 293 cells specifically expressing the E1a gene from adenovirus type 5. Source of transfected NCBP1 supplied by author, and identified by anti-tag western jblenis@hms.harvard.edu 27-Aug-2008: Contacted by JYOTI. davet Only protein-protein interactions 17 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ma XM., Yoon SO., Richardson CJ., Julich K., Blenis J. Apoptosis - Interactions involving proteins with a function related to apoptosis I have not attempted to capture the RNA binding data as it appears to have been done with a heterogeneous mRNA population. Author used 293E cells - a sub-clone of 293 cells specifically expressing the E1a gene from adenovirus type 5. Source of transfected NCBP1 supplied by author, and identified by anti-tag western jblenis@hms.harvard.edu Accepted 2008-AUG-14 by DAVET. 27-Aug-2008: Contacted by JYOTI. Insulin (mM) Rapamycin (ng/ml) Cell (0092-8674) 2008 davet Only protein-protein interactions 17 imex curation SKAR links pre-mRNA splicing to mTOR/S6K1-mediated enhanced translation efficiency of spliced mRNAs. SKAR links pre-mRNA splicing to mTOR/S6K1-mediated enhanced translation efficiency of spliced mRNAs. Cell (0092-8674) 2008 imex curation Ma XM., Yoon SO., Richardson CJ., Julich K., Blenis J. Apoptosis - Interactions involving proteins with a function related to apoptosis I have not attempted to capture the RNA binding data as it appears to have been done with a heterogeneous mRNA population. Author used 293E cells - a sub-clone of 293 cells specifically expressing the E1a gene from adenovirus type 5. Source of transfected NCBP1 supplied by author, and identified by anti-tag western jblenis@hms.harvard.edu 27-Aug-2008: Contacted by JYOTI. davet Only protein-protein interactions 17 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ma XM., Yoon SO., Richardson CJ., Julich K., Blenis J. Apoptosis - Interactions involving proteins with a function related to apoptosis I have not attempted to capture the RNA binding data as it appears to have been done with a heterogeneous mRNA population. Author used 293E cells - a sub-clone of 293 cells specifically expressing the E1a gene from adenovirus type 5. Source of transfected NCBP1 supplied by author, and identified by anti-tag western jblenis@hms.harvard.edu Accepted 2008-AUG-14 by DAVET. 27-Aug-2008: Contacted by JYOTI. Insulin (mM) Rapamycin (ng/ml) Cell (0092-8674) 2008 davet Only protein-protein interactions 17 imex curation SKAR links pre-mRNA splicing to mTOR/S6K1-mediated enhanced translation efficiency of spliced mRNAs. SKAR links pre-mRNA splicing to mTOR/S6K1-mediated enhanced translation efficiency of spliced mRNAs. Cell (0092-8674) 2008 imex curation Ma XM., Yoon SO., Richardson CJ., Julich K., Blenis J. Apoptosis - Interactions involving proteins with a function related to apoptosis I have not attempted to capture the RNA binding data as it appears to have been done with a heterogeneous mRNA population. Author used 293E cells - a sub-clone of 293 cells specifically expressing the E1a gene from adenovirus type 5. Source of transfected NCBP1 supplied by author, and identified by anti-tag western jblenis@hms.harvard.edu 27-Aug-2008: Contacted by JYOTI. davet Only protein-protein interactions 17 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ma XM., Yoon SO., Richardson CJ., Julich K., Blenis J. Apoptosis - Interactions involving proteins with a function related to apoptosis I have not attempted to capture the RNA binding data as it appears to have been done with a heterogeneous mRNA population. Author used 293E cells - a sub-clone of 293 cells specifically expressing the E1a gene from adenovirus type 5. Source of transfected NCBP1 supplied by author, and identified by anti-tag western jblenis@hms.harvard.edu Accepted 2008-AUG-14 by DAVET. Insulin (mM) Rapamycin (ng/ml) Cell (0092-8674) 2008 davet Only protein-protein interactions 17 imex curation SKAR links pre-mRNA splicing to mTOR/S6K1-mediated enhanced translation efficiency of spliced mRNAs. SKAR links pre-mRNA splicing to mTOR/S6K1-mediated enhanced translation efficiency of spliced mRNAs. Cell (0092-8674) 2008 imex curation Ma XM., Yoon SO., Richardson CJ., Julich K., Blenis J. Apoptosis - Interactions involving proteins with a function related to apoptosis I have not attempted to capture the RNA binding data as it appears to have been done with a heterogeneous mRNA population. Author used 293E cells - a sub-clone of 293 cells specifically expressing the E1a gene from adenovirus type 5. Source of transfected NCBP1 supplied by author, and identified by anti-tag western jblenis@hms.harvard.edu 27-Aug-2008: Contacted by JYOTI. davet Only protein-protein interactions 17 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ma XM., Yoon SO., Richardson CJ., Julich K., Blenis J. Apoptosis - Interactions involving proteins with a function related to apoptosis I have not attempted to capture the RNA binding data as it appears to have been done with a heterogeneous mRNA population. Author used 293E cells - a sub-clone of 293 cells specifically expressing the E1a gene from adenovirus type 5. Source of transfected NCBP1 supplied by author, and identified by anti-tag western jblenis@hms.harvard.edu Accepted 2008-AUG-14 by DAVET. 27-Aug-2008: Contacted by JYOTI. Insulin (mM) Rapamycin (ng/ml) Cell (0092-8674) 2008 davet Only protein-protein interactions 17 imex curation SKAR links pre-mRNA splicing to mTOR/S6K1-mediated enhanced translation efficiency of spliced mRNAs. SKAR links pre-mRNA splicing to mTOR/S6K1-mediated enhanced translation efficiency of spliced mRNAs. Cell (0092-8674) 2008 imex curation Ma XM., Yoon SO., Richardson CJ., Julich K., Blenis J. Apoptosis - Interactions involving proteins with a function related to apoptosis I have not attempted to capture the RNA binding data as it appears to have been done with a heterogeneous mRNA population. Author used 293E cells - a sub-clone of 293 cells specifically expressing the E1a gene from adenovirus type 5. Source of transfected NCBP1 supplied by author, and identified by anti-tag western jblenis@hms.harvard.edu 27-Aug-2008: Contacted by JYOTI. davet Only protein-protein interactions 17 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Ma XM., Yoon SO., Richardson CJ., Julich K., Blenis J. Apoptosis - Interactions involving proteins with a function related to apoptosis I have not attempted to capture the RNA binding data as it appears to have been done with a heterogeneous mRNA population. Author used 293E cells - a sub-clone of 293 cells specifically expressing the E1a gene from adenovirus type 5. Source of transfected NCBP1 supplied by author, and identified by anti-tag western jblenis@hms.harvard.edu Accepted 2008-AUG-14 by DAVET. 27-Aug-2008: Contacted by JYOTI. Insulin (mM) Rapamycin (ng/ml) Cell (0092-8674) 2008 davet Only protein-protein interactions 17 imex curation SKAR links pre-mRNA splicing to mTOR/S6K1-mediated enhanced translation efficiency of spliced mRNAs. SKAR links pre-mRNA splicing to mTOR/S6K1-mediated enhanced translation efficiency of spliced mRNAs. Cell (0092-8674) 2008 imex curation Ma XM., Yoon SO., Richardson CJ., Julich K., Blenis J. Apoptosis - Interactions involving proteins with a function related to apoptosis I have not attempted to capture the RNA binding data as it appears to have been done with a heterogeneous mRNA population. Author used 293E cells - a sub-clone of 293 cells specifically expressing the E1a gene from adenovirus type 5. Source of transfected NCBP1 supplied by author, and identified by anti-tag western jblenis@hms.harvard.edu 27-Aug-2008: Contacted by JYOTI. davet Only protein-protein interactions 17 human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Ma XM., Yoon SO., Richardson CJ., Julich K., Blenis J. Apoptosis - Interactions involving proteins with a function related to apoptosis I have not attempted to capture the RNA binding data as it appears to have been done with a heterogeneous mRNA population. Author used 293E cells - a sub-clone of 293 cells specifically expressing the E1a gene from adenovirus type 5. Source of transfected NCBP1 supplied by author, and identified by anti-tag western jblenis@hms.harvard.edu Accepted 2008-AUG-14 by DAVET. 27-Aug-2008: Contacted by JYOTI. Insulin (mM) Rapamycin (ng/ml) Cell (0092-8674) 2008 davet Only protein-protein interactions 17 imex curation if4e_human Eukaryotic translation initiation factor 4E mRNA cap-binding protein eIF-4F 25 kDa subunit EIF4E EIF4EL1 EIF4F protein protein human Homo sapiens Human MATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGSTTKNRFVV DIP protein P06730 original sequence version: 164 DIP protein P06730 original sequence version: 127 DIP protein P06730 original sequence version: 126 DIP protein P06730 original sequence version: 135 DIP protein P06730 original sequence version: 151 DIP protein P06730 original sequence version: 155 DIP protein P06730 original sequence version: 160 DIP protein P06730-1 original sequence version: 187 DIP protein P06730 original sequence version: 146 B869B8DE615E699D rs6_human 40S ribosomal protein S6 RPS6 Phosphoprotein NP33 Small ribosomal subunit protein eS6 OK/SW-cl.2 protein protein human Homo sapiens Human MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRKPLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLAKRMKEAKEKRQEQIAKRRRLSSLRASTSKSESSQK DIP protein P62753 original sequence version: 72 DIP protein P62753 original sequence version: 68 DIP protein P62753 original sequence version: 123 A61E435884E636AE pdip3_human Polymerase delta-interacting protein 3 POLDIP3 KIAA1649 PDIP46 46 kDa DNA polymerase delta interaction protein S6K1 Aly/REF-like target protein protein human Homo sapiens Human MADISLDELIRKRGAAAKGRLNARPGVGGVRSRVGIQQGLLSQSTRTATFQQRFDARQKIGLSDARLKLGVKDAREKLLQKDARFRIKGKVQDAREMLNSRKQQTTVPQKPRQVADAREKISLKRSSPAAFINPPIGTVTPALKLTKTIQVPQQKAMAPLHPHPAGMRINVVNNHQAKQNLYDLDEDDDGIASVPTKQMKFAASGGFLHHMAGLSSSKLSMSKALPLTKVVQNDAYTAPALPSSIRTKALTNMSRTLVNKEEPPKELPAAEPVLSPLEGTKMTVNNLHPRVTEEDIVELFCVCGALKRARLVHPGVAEVVFVKKDDAITAYKKYNNRCLDGQPMKCNLHMNGNVITSDQPILLRLSDSPSMKKESELPRRVNSASSSNPPAEVDPDTILKALFKSSGASVTTQPTEFKIKL 39A4A2362852E020 ncbp1_human Nuclear cap-binding protein subunit 1 NCBP1 CBP80 NCBP 80 kDa nuclear cap-binding protein protein protein human Homo sapiens Human MSRRRHSDENDGGQPHKRRKTSDANETEDHLESLICKVGEKSACSLESNLEGLAGVLEADLPNYKSKILRLLCTVARLLPEKLTIYTTLVGLLNARNYNFGGEFVEAMIRQLKESLKANNYNEAVYLVRFLSDLVNCHVIAAPSMVAMFENFVSVTQEEDVPQVRRDWYVYAFLSSLPWVGKELYEKKDAEMDRIFANTESYLKRRQKTHVPMLQVWTADKPHPQEEYLDCLWAQIQKLKKDRWQERHILRPYLAFDSILCEALQHNLPPFTPPPHTEDSVYPMPRVIFRMFDYTDDPEGPVMPGSHSVERFVIEENLHCIIKSHWKERKTCAAQLVSYPGKNKIPLNYHIVEVIFAELFQLPAPPHIDVMYTTLLIELCKLQPGSLPQVLAQATEMLYMRLDTMNTTCVDRFINWFSHHLSNFQFRWSWEDWSDCLSQDPESPKPKFVREVLEKCMRLSYHQRILDIVPPTFSALCPANPTCIYKYGDESSNSLPGHSVALCLAVAFKSKATNDEIFSILKDVPNPNQDDDDDEGFSFNPLKIEVFVQTLLHLAAKSFSHSFSALAKFHEVFKTLAESDEGKLHVLRVMFEVWRNHPQMIAVLVDKMIRTQIVDCAAVANWIFSSELSRDFTRLFVWEILHSTIRKMNKHVLKIQKELEEAKEKLARQHKRRSDDDDRSSDRKDGVLEEQIERLQEKVESAQSEQKNLFLVIFQRFIMILTEHLVRCETDGTSVLTPWYKNCIERLQQIFLQHHQIIQQYMVTLENLLFTAELDPHILAVFQQFCALQA DIP protein Q09161 original sequence version: 106 DIP protein Q09161 original sequence version: 135 F10DE7B9D16FDA0B if4a3_human Eukaryotic initiation factor 4A-III KIAA0111 EIF4A3 DDX48 Eukaryotic translation initiation factor 4A isoform 3 ATP-dependent RNA helicase eIF4A-3 ATP-dependent RNA helicase DDX48 DEAD box protein 48 Eukaryotic initiation factor 4A-like NUK-34 Nuclear matrix protein 265 protein protein human Homo sapiens Human MATTATMATSGSARKRLLKEEDMTKVEFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFSISVLQCLDIQVRETQALILAPTRELAVQIQKGLLALGDYMNVQCHACIGGTNVGEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRAIKMLVLDEADEMLNKGFKEQIYDVYRYLPPATQVVLISATLPHEILEMTNKFMTDPIRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMREANFTVSSMHGDMPQKERESIMKEFRSGASRVLISTDVWARGLDVPQVSLIINYDLPNNRELYIHRIGRSGRYGRKGVAINFVKNDDIRILRDIEQYYSTQIDEMPMNVADLI DIP protein P38919 original sequence version: 160 DIP protein P38919 original sequence version: 161 DIP protein P38919 original sequence version: 124 DIP protein P38919 original sequence version: 137 DIP protein P38919 original sequence version: 157 DIP protein P38919 original sequence version: 146 DIP protein P38919 original sequence version: 151 3A21888CA96CA5EA mtor_human Serine/threonine-protein kinase mTOR MTOR FRAP FRAP2 FK506-binding protein 12-rapamycin complex-associated protein 1 Rapamycin target protein 1 FRAP1 Mammalian target of rapamycin FKBP12-rapamycin complex-associated protein Mechanistic target of rapamycin RAFT1 RAPT1 Rapamycin and FKBP12 target 1 protein protein human Homo sapiens Human MLGTGPAAATTAATTSSNVSVLQQFASGLKSRNEETRAKAAKELQHYVTMELREMSQEESTRFYDQLNHHIFELVSSSDANERKGGILAIASLIGVEGGNATRIGRFANYLRNLLPSNDPVVMEMASKAIGRLAMAGDTFTAEYVEFEVKRALEWLGADRNEGRRHAAVLVLRELAISVPTFFFQQVQPFFDNIFVAVWDPKQAIREGAVAALRACLILTTQREPKEMQKPQWYRHTFEEAEKGFDETLAKEKGMNRDDRIHGALLILNELVRISSMEGERLREEMEEITQQQLVHDKYCKDLMGFGTKPRHITPFTSFQAVQPQQSNALVGLLGYSSHQGLMGFGTSPSPAKSTLVESRCCRDLMEEKFDQVCQWVLKCRNSKNSLIQMTILNLLPRLAAFRPSAFTDTQYLQDTMNHVLSCVKKEKERTAAFQALGLLSVAVRSEFKVYLPRVLDIIRAALPPKDFAHKRQKAMQVDATVFTCISMLARAMGPGIQQDIKELLEPMLAVGLSPALTAVLYDLSRQIPQLKKDIQDGLLKMLSLVLMHKPLRHPGMPKGLAHQLASPGLTTLPEASDVGSITLALRTLGSFEFEGHSLTQFVRHCADHFLNSEHKEIRMEAARTCSRLLTPSIHLISGHAHVVSQTAVQVVADVLSKLLVVGITDPDPDIRYCVLASLDERFDAHLAQAENLQALFVALNDQVFEIRELAICTVGRLSSMNPAFVMPFLRKMLIQILTELEHSGIGRIKEQSARMLGHLVSNAPRLIRPYMEPILKALILKLKDPDPDPNPGVINNVLATIGELAQVSGLEMRKWVDELFIIIMDMLQDSSLLAKRQVALWTLGQLVASTGYVVEPYRKYPTLLEVLLNFLKTEQNQGTRREAIRVLGLLGALDPYKHKVNIGMIDQSRDASAVSLSESKSSQDSSDYSTSEMLVNMGNLPLDEFYPAVSMVALMRIFRDQSLSHHHTMVVQAITFIFKSLGLKCVQFLPQVMPTFLNVIRVCDGAIREFLFQQLGMLVSFVKSHIRPYMDEIVTLMREFWVMNTSIQSTIILLIEQIVVALGGEFKLYLPQLIPHMLRVFMHDNSPGRIVSIKLLAAIQLFGANLDDYLHLLLPPIVKLFDAPEAPLPSRKAALETVDRLTESLDFTDYASRIIHPIVRTLDQSPELRSTAMDTLSSLVFQLGKKYQIFIPMVNKVLVRHRINHQRYDVLICRIVKGYTLADEEEDPLIYQHRMLRSGQGDALASGPVETGPMKKLHVSTINLQKAWGAARRVSKDDWLEWLRRLSLELLKDSSSPSLRSCWALAQAYNPMARDLFNAAFVSCWSELNEDQQDELIRSIELALTSQDIAEVTQTLLNLAEFMEHSDKGPLPLRDDNGIVLLGERAAKCRAYAKALHYKELEFQKGPTPAILESLISINNKLQQPEAAAGVLEYAMKHFGELEIQATWYEKLHEWEDALVAYDKKMDTNKDDPELMLGRMRCLEALGEWGQLHQQCCEKWTLVNDETQAKMARMAAAAAWGLGQWDSMEEYTCMIPRDTHDGAFYRAVLALHQDLFSLAQQCIDKARDLLDAELTAMAGESYSRAYGAMVSCHMLSELEEVIQYKLVPERREIIRQIWWERLQGCQRIVEDWQKILMVRSLVVSPHEDMRTWLKYASLCGKSGRLALAHKTLVLLLGVDPSRQLDHPLPTVHPQVTYAYMKNMWKSARKIDAFQHMQHFVQTMQQQAQHAIATEDQQHKQELHKLMARCFLKLGEWQLNLQGINESTIPKVLQYYSAATEHDRSWYKAWHAWAVMNFEAVLHYKHQNQARDEKKKLRHASGANITNATTAATTAATATTTASTEGSNSESEAESTENSPTPSPLQKKVTEDLSKTLLMYTVPAVQGFFRSISLSRGNNLQDTLRVLTLWFDYGHWPDVNEALVEGVKAIQIDTWLQVIPQLIARIDTPRPLVGRLIHQLLTDIGRYHPQALIYPLTVASKSTTTARHNAANKILKNMCEHSNTLVQQAMMVSEELIRVAILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQLPQLTSLELQYVSPKLLMCRDLELAVPGTYDPNQPIIRIQSIAPSLQVITSKQRPRKLTLMGSNGHEFVFLLKGHEDLRQDERVMQLFGLVNTLLANDPTSLRKNLSIQRYAVIPLSTNSGLIGWVPHCDTLHALIRDYREKKKILLNIEHRIMLRMAPDYDHLTLMQKVEVFEHAVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLAVMSMVGYILGLGDRHPSNLMLDRLSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLTNAMEVTGLDGNYRITCHTVMEVLREHKDSVMAVLEAFVYDPLLNWRLMDTNTKGNKRSRTRTDSYSAGQSVEILDGVELGEPAHKKTGTTVPESIHSFIGDGLVKPEALNKKAIQIINRVRDKLTGRDFSHDDTLDVPTQVELLIKQATSHENLCQCYIGWCPFW DIP protein P42345 original sequence version: 158 DIP protein P42345 original sequence version: 82 DIP protein P42345 original sequence version: 108 DIP protein P42345 original sequence version: 107 DIP protein P42345 original sequence version: 138 DIP protein P42345 original sequence version: 168 DIP protein P42345 original sequence version: 173 DIP protein P42345 original sequence version: 120 DIP protein P42345 original sequence version: 149 DIP protein P42345 original sequence version: 134 DIP protein P42345 original sequence version: 148 DIP protein P42345 original sequence version: 118 7D9AD6E784882AB4 ks6b1_human Ribosomal protein S6 kinase beta-1 RPS6KB1 STK14A Ribosomal protein S6 kinase I p70 ribosomal S6 kinase alpha 70 kDa ribosomal protein S6 kinase 1 Serine/threonine-protein kinase 14A protein protein human Homo sapiens Human MRRRRRRDGFYPAPDFRDREAEDMAGVFDIDLDQPEDAGSEDELEEGGQLNESMDHGGVGPYELGMEHCEKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVTGANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGENRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHPFFRHINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDSTLSESANQVFLGFTYVAPSVLESVKEKFSFEPKIRSPRRFIGSPRTPVSPVKFSPGDFWGRGASASTANPQTPVEYPMETSGIEQMDVTMSGEASAPLPIRQPNSGPYKKQAFPMISKRPEHLRMNL DIP protein P23443 original sequence version: 163 DIP protein P23443 original sequence version: 88 2C3BA13CCDAF4AB3 eif3b_human Eukaryotic translation initiation factor 3 subunit B Eukaryotic translation initiation factor 3 subunit 9 eIF-3-eta eIF3 p116 eIF3 p110 Prt1 homolog EIF3B EIF3S9 protein protein human Homo sapiens Human MQDAENVAVPEAAEERAEPGQQQPAAEPPPAEGLLRPAGPGAPEAAGTEASSEEVGIAEAGPESEVRTEPAAEAEAASGPSESPSPPAAEELPGSHAEPPVPAQGEAPGEQARDERSDSRAQAVSEDAGGNEGRAAEAEPRALENGDADEPSFSDPEDFVDDVSEEELLGDVLKDRPQEADGIDSVIVVDNVPQVGPDRLEKLKNVIHKIFSKFGKITNDFYPEEDGKTKGYIFLEYASPAHAVDAVKNADGYKLDKQHTFRVNLFTDFDKYMTISDEWDIPEKQPFKDLGNLRYWLEEAECRDQYSVIFESGDRTSIFWNDVKDPVSIEERARWTETYVRWSPKGTYLATFHQRGIALWGGEKFKQIQRFSHQGVQLIDFSPCERYLVTFSPLMDTQDDPQAIIIWDILTGHKKRGFHCESSAHWPIFKWSHDGKFFARMTLDTLSIYETPSMGLLDKKSLKISGIKDFSWSPGGNIIAFWVPEDKDIPARVTLMQLPTRQEIRVRNLFNVVDCKLHWQKNGDYLCVKVDRTPKGTQGVVTNFEIFRMREKQVPVDVVEMKETIIAFAWEPNGSKFAVLHGEAPRISVSFYHVKNNGKIELIKMFDKQQANTIFWSPQGQFVVLAGLRSMNGALAFVDTSDCTVMNIAEHYMASDVEWDPTGRYVVTSVSWWSHKVDNAYWLWTFQGRLLQKNNKDRFCQLLWRPRPPTLLSQEQIKQIKKDLKKYSKIFEQKDRLSQSKASKELVERRRTMMEDFRKYRKMAQELYMEQKNERLELRGGVDTDELDSNVDDWEEETIEFFVTEEIIPLGNQE DIP protein P55884 original sequence version: 84 C687A986EDAE0F5E srsf2_human Serine/arginine-rich splicing factor 2 Splicing factor SC35 Splicing component, 35 kDa Protein PR264 SRSF2 SFRS2 Splicing factor, arginine/serine-rich 2 protein protein human Homo sapiens Human MSYGRPPPDVEGMTSLKVDNLTYRTSPDTLRRVFEKYGRVGDVYIPRDRYTKESRGFAFVRFHDKRDAEDAMDAMDGAVLDGRELRVQMARYGRPPDSHHSRRGPPPRRYGGGGYGRRSRSPRRRRRSRSRSRSRSRSRSRSRYSRSKSRSRTRSRSRSTSKSRSARRSKSKSSSVSRSRSRSRSRSRSRSPPPVSKRESKSRSRSKSPPKSPEEEGAVSS DIP protein Q01130 original sequence version: 169 68121AC4D35714FA ren3b_human Regulator of nonsense transcripts 3B RENT3B Nonsense mRNA reducing factor 3B Up-frameshift suppressor 3 homolog B UPF3X UPF3B Up-frameshift suppressor 3 homolog on chromosome X protein protein human Homo sapiens Human MKEEKEHRPKEKRVTLLTPAGATGSGGGTSGDSSKGEDKQDRNKEKKEALSKVVIRRLPPTLTKEQLQEHLQPMPEHDYFEFFSNDTSLYPHMYARAYINFKNQEDIILFRDRFDGYVFLDNKGQEYPAIVEFAPFQKAAKKKTKKRDTKVGTIDDDPEYRKFLESYATDNEKMTSTPETLLEEIEAKNRELIAKKTTPLLSFLKNKQRMREEKREERRRREIERKRQREEERRKWKEEEKRKRKDIEKLKKIDRIPERDKLKDEPKIKVHRFLLQAVNQKNLLKKPEKGDEKELDKREKAKKLDKENLSDERASGQSCTLPKRSDSELKDEKPKRPEDESGRDYREREREYERDQERILRERERLKRQEEERRRQKERYEKEKTFKRKEEEMKKEKDTLRDKGKKAESTESIGSSEKTEKKEEVVKRDRIRNKDRPAMQLYQPGARSRNRLCPPDDSTKSGDSAAERKQESGISHRKEGGEE DIP protein Q9BZI7 original sequence version: 74 DIP protein Q9BZI7 original sequence version: 75 F5A8A395783D1A69 ncbp1-rps6-1 1 10 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey ser-240_ser244 (potential) opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 1A A number of other proteins were shown to be bound to NCBP1 by anti-phosphoserine Abs but none were actually identified. 100 mM insulin for 30 min 20 ng/mL rapamycin for 20 min + 100 mM insulin for 30 min Rapamycin inhibited insulin-induced phosphorylation of RPS6 eif3b-eif4e-1 3 10 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 13 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey association association Fig. 1B 100 20 Q09161 transfection eif3b-eif4e-2 3 7 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 1B 100 0 Q09161 transfection ncbp1-eif3b-1 2 13 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 12 unspecified role unspecified role prey prey association association Fig. 1B 100 20 ncbp1-eif3b-2 2 14 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 13 unspecified role unspecified role prey prey region nphosres N-phosphorylated residue NPhosRes undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 1B 100 0 Q09161 transfection RPS6KB1 is serine phosphorylated eif4e-eif3b 3 14 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey region nphosres N-phosphorylated residue NPhosRes undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role prey prey association association Fig. 1B 100 0 RPS6KB1 is weakly serine phosphorylated eif4e-eif3b-1 3 13 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey association association Fig. 1B 100 20 ncbp1-poldip3 4 16 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association The association of SKAR and Flag-Upf3b with CBP80-bound mRNPs was determined by immunoblotting. Fig. 2A Q09161; Q9BZI7 transfection ncbp1-poldip3-1 1 10 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association SKAR coimmunoprecipitates endogenous CBP80 Fig. 2B poldip3-sfrs2 5 15 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization Q9BY77 transfection Fig. 3A As shown in Figure 3A, SKAR significantly colocalizes with SC35, poldip3-sfrs2-1 5 15 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 3A As shown in Figure 3A, SKAR significantly colocalizes with SC35, poldip3-sfrs2-2 6 9 unspecified role unspecified role neutral component neutral component 15 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 3A As shown in Figure 3A, SKAR significantly colocalizes with SC35, eif4a3-poldip3-1 1 11 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey association association Interaction with NCBP1 is RNA-dependent As shown in Figure 4C, eIF4AIII was coimmunoprecipitated with SKAR, and the interaction was not disrupted by RNase digestion. Fig. 4C ncbp1-rps6-2 4 10 unspecified role unspecified role bait bait sbp tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey ser-240_ser244 (potential) opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 5A, Supp. Fig 2A, Supp. Fig. 2B A number of other proteins were shown to be bound to NCBP1 by anti-phosphoserine Abs but none were actually identified. siRNA studies showed POLDIP3 to be required for the insulin-stimulated increase in CBP80-bound phosphoproteins. Phosphorylation required RPS6KB1 but not RPS6KB2. Rapamycin inhibited insulin-induced phosphorylation of RPS6 100 mM insulin for 30 min 20 ng/mL rapamycin for 20 min + 100 mM insulin for 30 min eif3b-ncbp1-3 4 8 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association Fig. 5B 100 mM insulin for 30 mins 0 ng/ml rapamycin + 0 mM insulin RPS6 and RPS6KB1 showed insulin-dependent phosphorylation, siRNA studies suggest POLDIP3 is required for RPS6KB1 binding. eif3b-ncbp1-4 4 14 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association Fig. 5B 0 mM insulin 20 ng/ml rapamycin for 20 mins + 100 mM insulin for 30 mins siRNA studies suggested binding of RPSkB! was POLDIP3-dependent. rps6kb1-eif4e-6 1 13 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey association association Fig. 5B 100 mM insulin for 30 mins 0 ng/ml rapamycin + 0 mM insulin RPS6 was Ser-240 and Ser-244 phosphorylated. eif3b-eif4e-6 3 14 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 7 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey association association Fig. 1B 0 0 Q09161 transfection eif4e-eif3b-2 3 7 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey association association Fig. 1B 0 0 ncbp1-eif3b-3 2 13 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association Fig. 1B 0 0