IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Homer2 and Homer3 interact with amyloid precursor protein and inhibit Abeta production. Homer2 and Homer3 interact with amyloid precursor protein and inhibit Abeta production. Neurobiol. Dis. (0969-9961) 2008 imex curation Parisiadou L., Bethani I., Michaki V., Krousti K., Rapti G., Efthimiopoulos S. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Apoptosis - Interactions involving proteins with a function related to apoptosis Alzheimers - Interactions investigated in the context of Alzheimers disease Details on source of origin of HOMER proteins supplied by the author. efthis@biol.uoa.gr 03-Feb-2009: Contacted by JYOTI. jyoti Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Parisiadou L., Bethani I., Michaki V., Krousti K., Rapti G., Efthimiopoulos S. Apoptosis - Interactions involving proteins with a function related to apoptosis Details on source of origin of HOMER proteins supplied by the author. efthis@biol.uoa.gr Accepted 2008-Not a month!-12 by JYOTI. 03-Mar-2009: Contacted by JYOTI. Neurobiol. Dis. (0969-9961) 2008 jyoti Only protein-protein interactions imex curation Homer2 and Homer3 interact with amyloid precursor protein and inhibit Abeta production. Homer2 and Homer3 interact with amyloid precursor protein and inhibit Abeta production. Neurobiol. Dis. (0969-9961) 2008 imex curation Parisiadou L., Bethani I., Michaki V., Krousti K., Rapti G., Efthimiopoulos S. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Apoptosis - Interactions involving proteins with a function related to apoptosis Alzheimers - Interactions investigated in the context of Alzheimers disease Details on source of origin of HOMER proteins supplied by the author. efthis@biol.uoa.gr 03-Feb-2009: Contacted by JYOTI. jyoti Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Parisiadou L., Bethani I., Michaki V., Krousti K., Rapti G., Efthimiopoulos S. Apoptosis - Interactions involving proteins with a function related to apoptosis Details on source of origin of HOMER proteins supplied by the author. efthis@biol.uoa.gr Accepted 2008-Not a month!-12 by JYOTI. 03-Feb-2009: Contacted by JYOTI. Neurobiol. Dis. (0969-9961) 2008 jyoti Only protein-protein interactions imex curation p05067-8 Amyloid-beta precursor protein APP A4 AD1 ABPP Alzheimer disease amyloid protein Cerebral vascular amyloid peptide PreA4 Protease nexin-II APPI PreA4 751 Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human MLPGLALLLLAAWTARALEVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGCGGNRNNFDTEEYCMAVCGSAIPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHSFGADSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN A major isoform. C987C557C5A3714E home2_human Homer protein homolog 2 HOMER2 Cupidin protein protein human Homo sapiens Human MGEQPIFTTRAHVFQIDPNTKKNWMPASKQAVTVSYFYDVTRNSYRIISVDGAKVIINSTITPNMTFTKTSQKFGQWADSRANTVFGLGFSSEQQLTKFAEKFQEVKEAAKIAKDKTQEKIETSSNHSQESGRETPSSTQASSVNGTDDEKASHAGPANTHLKSENDKLKIALTQSAANVKKWEIELQTLRESNARLTTALQESAASVEQWKRQFSICRDENDRLRNKIDELEEQCSEINREKEKNTQLKRRIEELEAELREKETELKDLRKQSEIIPQLMSECEYVSEKLEAAERDNQNLEDKVRSLKTDIEESKYRQRHLKVELKSFLEVLDGKIDDLHDFRRGLSKLGTDN 33D258177F798373 home3_human Homer protein homolog 3 HOMER3 protein protein human Homo sapiens Human MSTAREQPIFSTRAHVFQIDPATKRNWIPAGKHALTVSYFYDATRNVYRIISIGGAKAIINSTVTPNMTFTKTSQKFGQWADSRANTVYGLGFASEQHLTQFAEKFQEVKEAARLAREKSQDGGELTSPALGLASHQVPPSPLVSANGPGEEKLFRSQSADAPGPTERERLKKMLSEGSVGEVQWEAEFFALQDSNNKLAGALREANAAAAQWRQQLEAQRAEAERLRQRVAELEAQAASEVTPTGEKEGLGQGQSLEQLEALVQTKDQEIQTLKSQTGGPREALEAAEREETQQKVQDLETRNAELEHQLRAMERSLEEARAERERARAEVGRAAQLLDVSLFELSELREGLARLAEAAP 9FCC62319FF5165A a4-homer3-1 1 3 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey evh1 region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q9NSC5:p.Trp27Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W A Wed May 02 19:15:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp27ala' Tue Jun 30 15:55:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp27Ala' region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Focusing initially on Homer3, we found that immunoprecipitation of APP751 with the R1(57) antibody (Fig. 1B, panel 1, lane 4) resulted in co-precipitation of Homer3 (Fig. 1B, panel 2, lane 4) Fig. 1B a4-homer3-2 2 5 unspecified role unspecified role bait bait evh1 region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q9NSC5:p.Trp27Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W A Wed May 02 15:24:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp27ala' Tue Jun 30 19:04:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp27Ala' region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role prey prey physical association physical association Focusing initially on Homer3, we found that immunoprecipitation of APP751 with the R1(57) antibody (Fig. 1B, panel 1, lane 4) resulted in co-precipitation of Homer3 (Fig. 1B, panel 2, lane 4) Fig. 1C a4-homer2-1 2 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Immunoprecipitation of Homer2 (Fig. 2, panel 1, lane 5) but not of Homer1 (Fig. 2, panel 1, lane 3) resulted in the co-precipitation of APP Fig. 2 a4-homer3-3 2 5 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association Focusing initially on Homer3, we found that immunoprecipitation of APP751 with the R1(57) antibody (Fig. 1B, panel 1, lane 4) resulted in co-precipitation of Homer3 (Fig. 1B, panel 2, lane 4) Fig. 3 In this case, immunoprecipitation of Homer3 (Fig. 3, panel 1, lanes 3 and 5), using the anti-myc antibody 9B11, resulted in the co-precipitation of wild type and deletion mutant APP (Fig. 3, panel 2, lanes 3 and 5).