IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Interaction between Transcription Factor, Basal Transcription Factor 3, and the NH2-Terminal Domain of Human Estrogen Receptor {alpha}. Interaction between Transcription Factor, Basal Transcription Factor 3, and the NH2-Terminal Domain of Human Estrogen Receptor {alpha}. Mol. Cancer Res. (1541-7786) 2007 imex curation Green CD., Thompson PD., Johnston PG., El-Tanani MK. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer m.el-tanani@qub.ac.uk. 22-Jan-2008: Contacted by JYOTI. A cDNA library constructed from EGF-stimulated MCF-7 human breast cancer cells was used. davet Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Green CD., Thompson PD., Johnston PG., El-Tanani MK. Apoptosis - Interactions involving proteins with a function related to apoptosis m.el-tanani@qub.ac.uk. Accepted 2007-Not a month!-5 by DAVET. A cDNA library constructed from EGF-stimulated MCF-7 human breast cancer cells was used. Mol. Cancer Res. (1541-7786) 2007 davet Only protein-protein interactions imex curation Interaction between Transcription Factor, Basal Transcription Factor 3, and the NH2-Terminal Domain of Human Estrogen Receptor {alpha}. Interaction between Transcription Factor, Basal Transcription Factor 3, and the NH2-Terminal Domain of Human Estrogen Receptor {alpha}. Mol. Cancer Res. (1541-7786) 2007 imex curation Green CD., Thompson PD., Johnston PG., El-Tanani MK. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer m.el-tanani@qub.ac.uk. 22-Jan-2008: Contacted by JYOTI. A cDNA library constructed from EGF-stimulated MCF-7 human breast cancer cells was used. davet Only protein-protein interactions human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Green CD., Thompson PD., Johnston PG., El-Tanani MK. Apoptosis - Interactions involving proteins with a function related to apoptosis m.el-tanani@qub.ac.uk. Accepted 2007-Not a month!-5 by DAVET. 22-Jan-2008: Contacted by JYOTI. A cDNA library constructed from EGF-stimulated MCF-7 human breast cancer cells was used. Mol. Cancer Res. (1541-7786) 2007 davet Only protein-protein interactions imex curation Interaction between Transcription Factor, Basal Transcription Factor 3, and the NH2-Terminal Domain of Human Estrogen Receptor {alpha}. Interaction between Transcription Factor, Basal Transcription Factor 3, and the NH2-Terminal Domain of Human Estrogen Receptor {alpha}. Mol. Cancer Res. (1541-7786) 2007 imex curation Green CD., Thompson PD., Johnston PG., El-Tanani MK. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer m.el-tanani@qub.ac.uk. 22-Jan-2008: Contacted by JYOTI. A cDNA library constructed from EGF-stimulated MCF-7 human breast cancer cells was used. davet Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Green CD., Thompson PD., Johnston PG., El-Tanani MK. Apoptosis - Interactions involving proteins with a function related to apoptosis m.el-tanani@qub.ac.uk. Accepted 2007-Not a month!-5 by DAVET. 22-Jan-2008: Contacted by JYOTI. A cDNA library constructed from EGF-stimulated MCF-7 human breast cancer cells was used. Mol. Cancer Res. (1541-7786) 2007 davet Only protein-protein interactions imex curation Interaction between Transcription Factor, Basal Transcription Factor 3, and the NH2-Terminal Domain of Human Estrogen Receptor {alpha}. Interaction between Transcription Factor, Basal Transcription Factor 3, and the NH2-Terminal Domain of Human Estrogen Receptor {alpha}. Mol. Cancer Res. (1541-7786) 2007 imex curation Green CD., Thompson PD., Johnston PG., El-Tanani MK. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer m.el-tanani@qub.ac.uk. 22-Jan-2008: Contacted by JYOTI. A cDNA library constructed from EGF-stimulated MCF-7 human breast cancer cells was used. davet Only protein-protein interactions human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Green CD., Thompson PD., Johnston PG., El-Tanani MK. Apoptosis - Interactions involving proteins with a function related to apoptosis m.el-tanani@qub.ac.uk. Accepted 2007-Not a month!-5 by DAVET. 22-Jan-2008: Contacted by JYOTI. A cDNA library constructed from EGF-stimulated MCF-7 human breast cancer cells was used. Mol. Cancer Res. (1541-7786) 2007 davet Only protein-protein interactions imex curation p53_mouse Cellular tumor antigen p53 Tp53 P53 Trp53 Tumor suppressor p53 protein protein mouse Mus musculus Mouse MTAMEESQSDISLELPLSQETFSGLWKLLPPEDILPSPHCMDDLLLPQDVEEFFEGPSEALRVSGAPAAQDPVTETPGPVAPAPATPWPLSSFVPSQKTYQGNYGFHLGFLQSGTAKSVMCTYSPPLNKLFCQLAKTCPVQLWVSATPPAGSRVRAMAIYKKSQHMTEVVRRCPHHERCSDGDGLAPPQHLIRVEGNLYPEYLEDRQTFRHSVVVPYEPPEAGSEYTTIHYKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRRTEEENFRKKEVLCPELPPGSAKRALPTCTSASPPQKKKPLDGEYFTLKIRGRKRFEMFRELNEALELKDAHATEESGDSRAHSSYLKTKKGQSTSRHKKTMVKKVGPDSD DIP protein P02340 original sequence version: 117 DIP protein P02340 original sequence version: 165 DIP protein P02340 original sequence version: 187 DIP protein P02340 original sequence version: 201 DIP protein P02340 original sequence version: 111 DIP protein P02340 original sequence version: 141 DIP protein P02340 original sequence version: 180 DIP protein P02340 original sequence version: 163 DIP protein P02340 original sequence version: 152 ED5A607378D921D7 lt_sv40 Large T antigen protein protein sv40 sv40 SV40 MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDKGGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLFCSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLICKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETKCDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQAVDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLPKMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLNFELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKRTQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALLLMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDDDEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFTCFKKPPTPPPEPET 3E37D4BAFC5D59BD esr1_human Estrogen receptor ESR1 ESR NR3A1 Estradiol receptor ER-alpha Nuclear receptor subfamily 3 group A member 1 protein protein human Homo sapiens Human MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV DIP protein P03372 original sequence version: 214 DIP protein P03372 original sequence version: 139 DIP protein P03372 original sequence version: 167 5455C57AB0CCCAA7 p20290-2 Transcription factor BTF3 BTF3b BTF3 NACB RNA polymerase B transcription factor 3 OK/SW-cl.8 Nascent polypeptide-associated complex subunit beta protein protein human Homo sapiens Human MKETIMNQEKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASKNEAN 5062DE8858CEE979 esr1-btf3 1 7 unspecified role unspecified role bait bait n-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey physical association physical association We have used the yeast two-hybrid system to isolate a cDNA coding for a protein that binds specifically to the AF-1 region of human ERA. This cDNA codes for the transcription factor basal transcription factor 3 (BTF3). esr1-btf3-1 3 7 unspecified role unspecified role bait bait n-terminal required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey physical association physical association These data indicate that BTF3b binds specifically to the region of the ER containing the AF-1 function. 2 A tp53-lt 3 5 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-1558391]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 390-390, sequence length 387 [EBI-1558391]72-390 6 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 2 A tp53-lt-1 2 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association 2 B esr1-btf3-2 2 7 unspecified role unspecified role bait bait n-terminal required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey physical association physical association 2 B Activation of the reporter gene was only detected when BTF3 was coexpressed with the A/B domain of ERa. btf3-esr1 4 8 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association This data clearly shows that ERa is specifically coimmunoprecipitated with BTF3b. 3 A P03372 In this experiment, coimmunoprecipitation of endogenous ERalpha when using BTF3 antibody was not observed. The authors suggest this is due to the association of unliganded ERalpha with HSPs, and the requirement for estradiol to dissociate this complex and stabilize the receptor. btf3-esr1-1 4 8 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association 3 C A strong interaction between endogenous ERa and BTF3 was observed in both estradiol-treated and untreated MCF-7 samples Estradiol - the association between ERa and BTF3 was significantly enhanced by the presence of estradiol.