IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Regulation of insulin secretion by SIRT4, a mitochondrial ADP-ribosyltransferase. Regulation of insulin secretion by SIRT4, a mitochondrial ADP-ribosyltransferase. The Journal of Biological Chemistry 2007 imex curation Nidhi Ahuja, Bjoern Schwer, Stefania Carobbio, David Waltregny, Brian J. North, Vincenzo Castronovo, Pierre Maechler, Eric Verdin Apoptosis - Interactions involving proteins with a function related to apoptosis everdin@gladstone.ucsf.edu. 29-Mar-2010: Contacted by JYOTI. Only protein-protein interactions Curation by: Molecular Connections Pvt. Ltd human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Nidhi Ahuja, Bjoern Schwer, Stefania Carobbio, David Waltregny, Brian J. North, Vincenzo Castronovo, Pierre Maechler, Eric Verdin Apoptosis - Interactions involving proteins with a function related to apoptosis everdin@gladstone.ucsf.edu. Accepted 2010-MAR-02 by SKERRIEN. 29-Mar-2010: Contacted by JYOTI. The Journal of Biological Chemistry 2007 Only protein-protein interactions imex curation Curation by: Molecular Connections Pvt. Ltd Regulation of insulin secretion by SIRT4, a mitochondrial ADP-ribosyltransferase. Regulation of insulin secretion by SIRT4, a mitochondrial ADP-ribosyltransferase. The Journal of Biological Chemistry 2007 imex curation Nidhi Ahuja, Bjoern Schwer, Stefania Carobbio, David Waltregny, Brian J. North, Vincenzo Castronovo, Pierre Maechler, Eric Verdin Apoptosis - Interactions involving proteins with a function related to apoptosis everdin@gladstone.ucsf.edu. 29-Mar-2010: Contacted by JYOTI. Only protein-protein interactions Curation by: Molecular Connections Pvt. Ltd human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip fingerprinting peptide massfingerprinting fingerprinting Nidhi Ahuja, Bjoern Schwer, Stefania Carobbio, David Waltregny, Brian J. North, Vincenzo Castronovo, Pierre Maechler, Eric Verdin Apoptosis - Interactions involving proteins with a function related to apoptosis everdin@gladstone.ucsf.edu. Accepted 2010-MAR-02 by SKERRIEN. 29-Mar-2010: Contacted by JYOTI. The Journal of Biological Chemistry 2007 Only protein-protein interactions imex curation Curation by: Molecular Connections Pvt. Ltd adt3_human ADP/ATP translocase 3 SLC25A6 ANT3 CDABP0051 Adenine nucleotide translocator 3 ADP,ATP carrier protein 3 Solute carrier family 25 member 6 ADP,ATP carrier protein, isoform T2 AAC3 protein protein human Homo sapiens Human MTEQAISFAKDFLAGGIAAAISKTAVAPIERVKLLLQVQHASKQIAADKQYKGIVDCIVRIPKEQGVLSFWRGNLANVIRYFPTQALNFAFKDKYKQIFLGGVDKHTQFWRYFAGNLASGGAAGATSLCFVYPLDFARTRLAADVGKSGTEREFRGLGDCLVKITKSDGIRGLYQGFSVSVQGIIIYRAAYFGVYDTAKGMLPDPKNTHIVVSWMIAQTVTAVAGVVSYPFDTVRRRMMMQSGRKGADIMYTGTVDCWRKIFRDEGGKAFFKGAWSNVLRGMGGAFVLVLYDELKKVI 18534E9F0E49672F sir4_human NAD-dependent protein lipoamidase sirtuin-4, mitochondrial SIR2L4 SIR2-like protein 4 SIRT4 NAD-dependent protein deacetylase sirtuin-4 NAD-dependent ADP-ribosyltransferase sirtuin-4 Regulatory protein SIR2 homolog 4 protein protein human Homo sapiens Human MKMSFALTFRSAKGRWIANPSQPCSKASIGLFVPASPPLDPEKVKELQRFITLSKRLLVMTGAGISTESGIPDYRSEKVGLYARTDRRPIQHGDFVRSAPIRQRYWARNFVGWPQFSSHQPNPAHWALSTWEKLGKLYWLVTQNVDALHTKAGSRRLTELHGCMDRVLCLDCGEQTPRGVLQERFQVLNPTWSAEAHGLAPDGDVFLSEEQVRSFQVPTCVQCGGHLKPDVVFFGDTVNPDKVDFVHKRVKEADSLLVVGSSLQVYSGYRFILTAWEKKLPIAILNIGPTRSDDLACLKLNSRCGELLPLIDPC 2B76963AD2C3B354 ide_human Insulin-degrading enzyme IDE Insulin protease Insulysin Abeta-degrading protease protein protein human Homo sapiens Human MRYRLAWLLHPALPSTFRSVLGARLPPPERLCGFQKKTYSKMNNPAIKRIGNHITKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSAYYSSNLMAVCVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPTKNEFIPTNFEILPLEKEATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL 8A28AEF75EDA0EDA adt2_human ADP/ATP translocase 2 SLC25A5 ANT2 Adenine nucleotide translocator 2 ADP,ATP carrier protein 2 Solute carrier family 25 member 5 ADP,ATP carrier protein, fibroblast isoform AAC2 protein protein human Homo sapiens Human MTDAAVSFAKDFLAGGVAAAISKTAVAPIERVKLLLQVQHASKQITADKQYKGIIDCVVRIPKEQGVLSFWRGNLANVIRYFPTQALNFAFKDKYKQIFLGGVDKRTQFWLYFAGNLASGGAAGATSLCFVYPLDFARTRLAADVGKAGAEREFRGLGDCLVKIYKSDGIKGLYQGFNVSVQGIIIYRAAYFGIYDTAKGMLPDPKNTHIVISWMIAQTVTAVAGLTSYPFDTVRRRMMMQSGRKGTDIMYTGTLDCWRKIARDEGGKAFFKGAWSNVLRGMGGAFVLVLYDEIKKYT DIP protein P05141 original sequence version: 132 DC53D083E7217AFE sir3_human NAD-dependent protein deacetylase sirtuin-3, mitochondrial SIRT3 SIR2L3 SIR2-like protein 3 Regulatory protein SIR2 homolog 3 protein protein human Homo sapiens Human MAFWGWRAAAALRLWGRVVERVEAGGGVGPFQACGCRLVLGGRDDVSAGLRGSHGARGEPLDPARPLQRPPRPEVPRAFRRQPRAAAPSFFFSSIKGGRRSISFSVGASSVVGSGGSSDKGKLSLQDVAELIRARACQRVVVMVGAGISTPSGIPDFRSPGSGLYSNLQQYDLPYPEAIFELPFFFHNPKPFFTLAKELYPGNYKPNVTHYFLRLLHDKGLLLRLYTQNIDGLERVSGIPASKLVEAHGTFASATCTVCQRPFPGEDIRADVMADRVPRCPVCTGVVKPDIVFFGEPLPQRFLLHVVDFPMADLLLILGTSLEVEPFASLTEAVRSSVPRLLINRDLVGPLAWHPRSRDVAQLGDVVHGVESLVELLGWTEEMRDLVQRETGKLDGPDK DIP protein Q9NTG7 original sequence version: 123 DIP protein Q9NTG7 original sequence version: 80 4BA8BD3AC5FC7901 sirt4-slc25a5 2 5 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey physical association physical association Supplemental Materials sirt4-ide-1 1 7 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig 5A sirt4-slc25a5-1 1 6 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 5B