IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Two functionally distinct Axin-like proteins regulate canonical Wnt signaling in C. elegans. Two functionally distinct Axin-like proteins regulate canonical Wnt signaling in C. elegans. Dev. Biol. (0012-1606) 2007 imex curation Oosterveen T., Coudreuse DY., Yang PT., Fraser E., Bergsma J., Dale TC., Korswagen HC. Apoptosis - Interactions involving proteins with a function related to apoptosis rkors@niob.knaw.nl A mixed-stage C. elegans cDNA library (Walhout et al., 2000, Science 287 (2000), pp. 116–122. jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Oosterveen T., Coudreuse DY., Yang PT., Fraser E., Bergsma J., Dale TC., Korswagen HC. Apoptosis - Interactions involving proteins with a function related to apoptosis rkors@niob.knaw.nl Accepted 2011-APR-19 by JYOTI A mixed-stage C. elegans cDNA library (Walhout et al., 2000, Science 287 (2000), pp. 116–122. Dev. Biol. (0012-1606) 2007 jyoti Only protein-protein interactions imex curation Two functionally distinct Axin-like proteins regulate canonical Wnt signaling in C. elegans. Two functionally distinct Axin-like proteins regulate canonical Wnt signaling in C. elegans. Dev. Biol. (0012-1606) 2007 imex curation Oosterveen T., Coudreuse DY., Yang PT., Fraser E., Bergsma J., Dale TC., Korswagen HC. Apoptosis - Interactions involving proteins with a function related to apoptosis rkors@niob.knaw.nl A mixed-stage C. elegans cDNA library (Walhout et al., 2000, Science 287 (2000), pp. 116–122. jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Oosterveen T., Coudreuse DY., Yang PT., Fraser E., Bergsma J., Dale TC., Korswagen HC. Apoptosis - Interactions involving proteins with a function related to apoptosis there are 3/4 trembl entries for mig-5 corresponding to the Gene ID - none are 400 aa's in length. Korswagen -2002 gives a link to Z66500 (a cosmid) - which eventually gives Q22227. Korswagen-2002 states "were checked by sequencing." - so presumably this applies here for Participant detection. The axl-1 cDNA was cloned into the Gal4 activation domain = PREY (containing vector pPC86 and was tested for interactions with other components of the BAR-1 destruction complex) MIG-5 has been entered as Q22227, though this has not been confirmed. rkors@niob.knaw.nl Accepted 2011-APR-19 by JYOTI A mixed-stage C. elegans cDNA library (Walhout et al., 2000, Science 287 (2000), pp. 116–122. Dev. Biol. (0012-1606) 2007 jyoti Only protein-protein interactions imex curation Two functionally distinct Axin-like proteins regulate canonical Wnt signaling in C. elegans. Two functionally distinct Axin-like proteins regulate canonical Wnt signaling in C. elegans. Dev. Biol. (0012-1606) 2007 imex curation Oosterveen T., Coudreuse DY., Yang PT., Fraser E., Bergsma J., Dale TC., Korswagen HC. Apoptosis - Interactions involving proteins with a function related to apoptosis rkors@niob.knaw.nl A mixed-stage C. elegans cDNA library (Walhout et al., 2000, Science 287 (2000), pp. 116–122. jyoti Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Oosterveen T., Coudreuse DY., Yang PT., Fraser E., Bergsma J., Dale TC., Korswagen HC. Apoptosis - Interactions involving proteins with a function related to apoptosis rkors@niob.knaw.nl Accepted 2011-APR-19 by JYOTI A mixed-stage C. elegans cDNA library (Walhout et al., 2000, Science 287 (2000), pp. 116–122. Dev. Biol. (0012-1606) 2007 jyoti Only protein-protein interactions imex curation gsk3_caeel Glycogen synthase kinase-3 gsk-3 sgg-1 Y18D10A.5 protein protein caeel Caenorhabditis elegans MNKQLLSCSLKSGKQVTMVVASVATDGVDQQVEISYYDQKVIGNGSFGVVFLAKLSTTNEMVAIKKVLQDKRFKNRELQIMRKLNHPNIVKLKYFFYSSGEKKDELYLNLILEYVPETVYRVARHYSKQRQQIPMIYVKLYMYQLLRSLAYIHSIGICHRDIKPQNLLIDPESGVLKLCDFGSAKYLVRNEPNVSYICSRYYRAPELIFGATNYTNSIDVWSAGTVMAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIQSMNPNYKEFKFPQIKAHPWNKVFRVHTPAEAIDLISKIIEYTPTSRPTPQAACQHAFFDELRNPDARLPSGRPLPTLEMDGPMGTGEVSTTSGDVAGPSA ABDD2F49CC475BF5 bar1_caeel Beta-catenin/armadillo-related protein 1 bar-1 pvl-1 spy-1 Protruding vulva protein 1 Suppressor of polyray 1 C54D1.6 protein protein caeel Caenorhabditis elegans MDLDPNLVINHDDTNLSEASFTMEQHTSSYSDIHMGSTPCTGHRKVDMWRNHNFDSGFQTMNHSEAPSIISSLHPSSHLSGMSSMADYEPIPTLSDQQKQKFDGITQNQADGQYNTVRAIPELTMLMKDQDNEVVHKAVILMQNIAKMECDPMRRQNEARIVDPRVIFTLRDLLRDKVEFPNIIRCTLGTFFHICNRQEGIDLVTRAIAEQPDIIPNLIRHIGTYPSSIYKYAILTMHSILSDKQRGGQSVIIARQQDAITHVTPWLEAEKSEKLLPVIVDLIRVLCEKNTEQKIKFVKMGGPQKLLMLLQHRVYENLLWRTTQLLKTFSNFDAPNLVAFGGRQILANLLSHGSPRLVQSTLETLRNISDVPSKIKEDLLLKSLLELVNSRNTTIRLYSAQIMSNLVANNRHNKEFMCGNNGVVILVRALTIATKEMGDLRDKEAQQMEDYIESLICTLRHLCVGHPMSDKVQAFVFRDPALFLHKLLTMRPVLLKHTLSLLLKVVSQHALLAPFRSCRIGDKGFVEQLIHILRVACTQLNVQESIEGVRVKDIIHLCIQILRWITRDQDILNEVVFFLQTPENSRMGDGHTLPIFVLQKANVEENTKSSALALIYNLMHHEQMANVLDRDDVLVKMLQNVQMQSQTHPELASLANNILKMMYEKREKTRNTLPRYNSYLESQFGHMSMTTPRSEALNSSGEVCEGAGEQWSTPLTDDTMMDSYCNSSGRDSSKPYNSPMYHSPPAMYPEYSIGPPETYLDPHATASCYPRPTPPQYNSYDRSPPVYNDLPSNPGPSSHSSDYYPSRNSRF 97D6FFDE71BDFDFF q18239_caeel dsh-2 CELE_C27A2.6 C27A2.6 protein protein caeel Caenorhabditis elegans MTDSPSPIDSSFDASDVATPCTVIAAKISLRNRNGLEEDQENLDSFDAFTETHETQESKNIAHGEHEEDVSNIYVDDFSKEFGDTVSSVMEPLPKPLTFARTITKVYCHLDDQEHPYMVEVHVPPDCITLRDVKRKLMRTNFKYYCIALDPDTGLEVKAEVRDDSRRLYPLKNGRFELYLLTVEGSVHSDTSSGRHRRKQDGSSKGSSGSREYLRAAHHYDNPTPFSDDESQASSLPTYVKKAHAYNRKHAPQAYERHLPHMKHNNRHNHHRQNHYEESTFDVTTESDDHYRDGVTYYDEDEDDSRSINTDLTSVSQVHLKQKWRQQQKEMRNKWKRMPSISTASSSFSSITESSMGLEVITVRLNLETIPLGMTPSGHTNARGDAGLYVGDIQDRGAVALDGRIDIGDMIVGINEISLGNYSNKEAVQLLREAVQRQYLTLTIAKTGDPKQNAFPRNPRAEPIRPIDPNEWVKHATNAMKAMPSISEESSSTPIPDDWPTNSSASGTPFGGPPPANCLNVMTDKKYVVEVMAAPGSGLDIKDRYWFKIPIPMSFLGTDLVEWLVKHVQGLETKKKAREFAEEMLKLGYIRPGVGKQSFTKECYYVMGDECADYTQLRGPDGGYKYPQSHASSASGHSSNNLIFPPSMYPPQPPTAGAVHHRNSVILPSSMTGGYASLPASPFPHGKASVNDCRRTRDDQRSQASGSSRGSSRRYVELPRKPASLSSGSGLAVDLDRVASRSSFRAAMNGSHPQFNIDN C18BDC42480D9749 hmp2_caeel Beta-catenin-like protein hmp-2 hmp-2 Protein humpback-2 K05C4.6 protein protein caeel Caenorhabditis elegans MLLHSTNSYSIFTDHEVETRTSRIRSAMFPDWIPPTSAAEATNSTTSIVEMMQMPTQQLKQSVMDLLTYEGSNDMSGLSLPDLVKLMCDHDESVVARAVHRAYMLSREDPNFFNAPGFDHRSFVEALMAASKSSNVNVRRNAIGALSHMSEQRGGPLLIFRSGGLAEIIRMLYDSLESVVHYAVTTLRNLLMHVSDSRAQARALNAVEALTPHLHKTNPKLLAQVADGLYFLLIDDAPSKITFLSLLGPQILVSILREYSDHRKLIYTVVRCIRSLSVCPSNKPALISLGCLPALYVELCTAKDERSQTAILVAMRNLSDSATNEENLTQLIIKLLEIIRVANDGMTACACGTLSNLTCNNTRNKQTVCSHGGIDALVTAIRRLPEVEEVTEPALCALRHCTARHSLAEEAQSELRFCQAFPVILDQLETLRTPVIKAALGVIRNSALLQTNLIELTQEQTANGHTAVSLTMDILRRAITAIEENPDIAVDGVPMWGVIEGAVSALHQLANHPAVAAACCDDIGQVGNPECPPFLDLLHRLLAHPRLGSMDDEVLEREILGLLYQLSKRPDGARAVESTGVSALLMESRGSQYKSVVTYANGVLSNLKRGDSAAIMNMSNSYDYEMSGSAADWQRDGLERELFAEMYPTNDGGHSESINMALNNSQMRPNHNWYDTDL DIP protein O44326 original sequence version: 125 E6C7ED51F6241232 gsk3b_human Glycogen synthase kinase-3 beta GSK3B Serine/threonine-protein kinase GSK3B protein protein human Homo sapiens Human MSGRPRTTSFAESCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASTPTNATAASDANTGDRGQTNNAASASASNST DIP protein P49841 original sequence version: 109 DIP protein P49841 original sequence version: 136 DIP protein P49841 original sequence version: 143 DIP protein P49841 original sequence version: 141 DIP protein P49841 original sequence version: 196 DIP protein P49841 original sequence version: 159 DIP protein P49841 original sequence version: 151 4ACC24D00CDBB9C3 mig5_caeel Segment polarity protein dishevelled homolog mig-5 Abnormal cell migration protein 5 T05C12.6 mig-5 protein protein caeel Caenorhabditis elegans MEPPCTSDCSQIKVFYYLDDETTPYVSVIEAREGVATLGNFKNSFTKRGYKYYAKELDPDIQREVKVELTTDSDRLRKSQNGFYEIFLVSTPGYGTLPRNSGTMTRPQRTALDKRRRRSADFDATPYSDASLAPSTIVSRRAGEHLAELYTSNSEDPYQYDEHTRRTGDDSSLYEPLAARDMNKIYDDDRRRKKQKKERFRRPYVPSTISSATESSVNSGLPRILEIYLPMKNVPYLGLSVCTIDGHIFVSEIAPEGAVEKDGRVNVGDQILQVNRVSFEELSGPQAVRSLREAASSKRPITLYISKFARGAPSEYDDPLASMASETMPLDVGVWVETAVQNTEKMKALGLDPQEQTATTIDDGTLPFTSTASDDEERMLYDQRRNGIPRALIEEAERKRENEQNEKIEQLTEMIDPIIVVRSMARPDSGLAVKNRKWLKILVPMSFIGRDLVDWLVDHMADIHNRKKARIYAARLLAAGLIRHVVSKLTFTEKCYYVFGDGILGNDRNSTDTTGTSGTTMRVEATTEVTYVGSPAPHALAARVGRNIPHRLETTTLSPVAHDQTWLRRRRDCESPMTNDYASMVGESQIGMNPVGNYHVFGTKNNHRQVPAPSQVTSSSLTNGSGGLGGPPPTPLSSTMVLAASPIQSQNAVNHDFDGENSSNSRTRILRT 198FA92312997CF6 apr1_caeel Adenomatous polyposis coli protein-related protein 1 apr-1 K04G2.8 protein protein caeel Caenorhabditis elegans MSSSSSDENETTIHRTGSNTGGSGIYSQPRAGSSKRTSNVRHDVSDVDDEEEHYARFREDTAIEVDDAITVLLSSLHFEHKRDIVPTDEDDNKLRELHEKIFALITSESDVNRKRRLKKALPASNCVREQVYYLRRKPSTPPASYYHRLNAALHTIVKESFGEEYRKVATVLGLVEALAEVLILEVHTFGINETNPGEHRNIRKLIANALTNLTYGQIHSKRRLCSYDGFIRCVVRIVIESPNITQVYAGLIRNLSWNADSGMSEALQPTVHALSIAAVHAHTHRFDVTATLSALWNLAGHSVENKRTICDTPNCLKVLASLLSPDARFTSLVDSATGILKYVSQYLANTSTHLELRSLLITRMLTLLKSASFTCVTNTLGAIANLIVKDPHMQQMIRQDMAAVQQLNVLRNSNRDDIRTAVKSVLNTLNQPCSHRYGDMSHSVGGGATGMQMLSEPQLQMQTSHHAYHGTASPRLLSLRATRASPGKYIQPQAQQQLIQTPQVDQRSSSLPRHFAVQRNGFVMAQSYNQQMDQHQQQQMIYQLQQQQQIMFQTEDQAQMEHHQQIMYLQQQQQQFHQIQQQQQMQKAQEADPVPPTDDDLDIPTSTVMGTRSNSERSLGSMNPGSVMTNWNSSLDTAANSSRALSPVSYNDIPASPTMCAQVFNLPKSTESEHHQLTSQQQNTTHYSSGSANTMTRSDGATTVPMDNIITPTYAILNPILVHEQTPNGTVPRKTSEELDSPDDVLPGPSLEEEEGDYAIIGGAAQKTDDELLTRSIQSEMPTSSSTPKMKVSPRLNGFFSPTQKTTSSPAWSHPDTSPIPKSSSHRTQPNRRQDASDADRLLMESIMSEMPKSRIISPRLAGTQQYLEPEPERRSHSKNEEADRRDAFTASHEPSDHNGIDVARGSDWSPQQQLHRMESLESQASSEDSFGLTAEEPNSSTSGAAANTMRFDDEIDASLPMDCVDDDDYDYTYDHFEDYEDEEDPDATQFDDGVDAQLTIDCSMISSGSGSSQRNETTTTSRDSKALATSTPKGSASSLPGVRQATRVSTNGKSRLPVPKTNGSLVDKNPKPIIASRRPRLPPKPTLLKDKHYPEEDSIENQTRDDTIYVNAPVVEAEQERIYMNALKQQKNIEQSPSIGNGSPIAKSAIVTPYNYQKPPFTGRNNGEMSNEKSVTPNPKQMLVTIV DIP protein Q21227 original sequence version: 65 F3CDA53B5D3527A0 pry1_caeel Axin-like protein pry-1 pry-1 Protein polyray C37A5.9 protein protein caeel Caenorhabditis elegans METHLGWARSLEAVLSDRSALDAFQEWLIEYSSPQYLDLFFAIRAYERMALEGKPEKSQLSKSIYSKFLSSRTGNCEAIPKHFRAPIGEKLRHGTELEDRVFSHCSNFVQEFLRRQHEEFVGSEEFIEAFNKMSSTTADQLPGGSAHHSSHQNTMRRSSGTTSRKSAAQIATQLTAEALLKSKHDRHSKLGETKLEKMYPPTRQPYVCNATTSHNDSAVSSTFSGDTPEAHRMHSNRLRHIRDEQARENHGTMTLPRVEKASVDGQQWDHSSESGRRNFAMEITRKLLRHIDKVKLNDEMEKRIDDIEECRYTTIDMVNGTEPNDDLGKIDEDEELDDYLKMKMTDDSQKGSTNRSPKGPAGEPNKSGEGSKNTTLSPTNRAPAQLHNTIRVPRRKDYPRDTSASLKSHRHHQIDTNRMMSQSMCAPSYSSASSSYSRDSFAPAPTTRVNFAPGSSKSSQFYDSSGIGSMAPSAFSATSSLDYKDRRQHRKAPTPKKHSKIGKNLSNLITISYLGTDKIPVVTHVPNDGPMTLAEFKRHFALPNGAHQLFFKTECEDGSAPFQLLLIKDEHHLLPVFEGRIAAELR 366D09FFC1568AE1 axin1_human Axin-1 Axis inhibition protein 1 AXIN1 AXIN protein protein human Homo sapiens Human MNIQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDPRPASYSFCSGKGVGIKGETSTATPRRSDLDLGYEPEGSASPTPPYLKWAESLHSLLDDQDGISLFRTFLKQEGCADLLDFWFACTGFRKLEPCDSNEEKRLKLARAIYRKYILDNNGIVSRQTKPATKSFIKGCIMKQLIDPAMFDQAQTEIQATMEENTYPSFLKSDIYLEYTRTGSESPKVCSDQSSGSGTGKGISGYLPTLNEDEEWKCDQDMDEDDGRDAAPPGRLPQKLLLETAAPRVSSSRRYSEGREFRYGSWREPVNPYYVNAGYALAPATSANDSEQQSLSSDADTLSLTDSSVDGIPPYRIRKQHRREMQESVQVNGRVPLPHIPRTYRVPKEVRVEPQKFAEELIHRLEAVQRTREAEEKLEERLKRVRMEEEGEDGDPSSGPPGPCHKLPPAPAWHHFPPRCVDMGCAGLRDAHEENPESILDEHVQRVLRTPGRQSPGPGHRSPDSGHVAKMPVALGGAASGHGKHVPKSGAKLDAAGLHHHRHVHHHVHHSTARPKEQVEAEATRRAQSSFAWGLEPHSHGARSRGYSESVGAAPNASDGLAHSGKVGVACKRNAKKAESGKSASTEVPGASEDAEKNQKIMQWIIEGEKEISRHRRTGHGSSGTRKPQPHENSRPLSLEHPWAGPQLRTSVQPSHLFIQDPTMPPHPAPNPLTQLEEARRRLEEEEKRASRAPSKQRYVQEVMRRGRACVRPACAPVLHVVPAVSDMELSETETRSQRKVGGGSAQPCDSIVVAYYFCGEPIPYRTLVRGRAVTLGQFKELLTKKGSYRYYFKKVSDEFDCGVVFEEVREDEAVLPVFEEKIIGKVEKVD DIP protein O15169 original sequence version: 118 DIP protein O15169 original sequence version: 125 DIP protein O15169-1 original sequence version: 140 DIP protein O15169 original sequence version: 132 10779173F5092F3F hmr1_caeel Cadherin-related hmr-1 hmr-1 Protein Hammerhead W02B9.1 protein protein caeel Caenorhabditis elegans MSWNILLILLISNLDEVLAKTLLKLPSNAPPGWLISDLQFQNLIGDSEIATLQPSIFSTNFEVEDGYRIITNTTVTQFHGELFELFLNVKEQNFQRLVTLHVYVDPRGTSQQPATFLSTVYHATVYTSQQPGSTVVFSKPITVRNRKNFVISPISKIDKISKYSSPFSVMTRGKSVDIVMMKQKLEEDDITRHVIFLGAFTEKTGEMIAQTKVIIDVIDSGDVHFLLKSKKSIAKFASAIPANSTVFDVEKRNLSEPLLFHLEEPSRFFKIDQFSGRVSTVLPVGYGTYHIHVVARNQKKQRSDAWLEISVKKEQKLEPMTSSRSRRHLDDIVFRIPENTTMEDIEKKDMKIPLFAGETIGEINVAKEWLKIDDDGKIHLLKPLNYEKTSSIIATVPINGLQSTRTQTIRIHVADIDEPPSFVNSPLPMLAVVPLNPTIGRIVYQFVARDEHGDGDSNVLYKTIDVIPAGSFIVDPKSGVVRTGWSKYERGDTYRISAQAMDLSPSDNTTSQLSEVAILEILADERPPQFAKQEYEVTVSEDNLVDYSVVDVKAQSFRSFEDGRSKGPITYSLEGDTPEDETKWFRIDPSTGIIHLTRLLDFDDPALPKLHKLKVTAREDNRESHVDLTIRIDDVNDNVPTFTRPLYTAQVREDIPLNQTILKVTAVDKDTGDNSRITYSVDNHNFSINSNGEISAKVRLDADQLNERHFVYRFNVTARDHGEPVSLSSSAMIHIRTENTNDESAVFLPTSQYTAFVAEDAQGGTPVIQIQARDADRDEVTYSFMDKNGRSTQKMNLFSIDEHTGLVKLRHGVSAADLAEAENPINLTVIVQDDGSCCVYPSKTHTSYATLLIGIEDVNNNKPEFPDCAKYSDIAKIMEGTYKTDPPTIVKVEATDDDSSANGDIVYSLYYTQSESRKAFVIDRQTGVLTPSPHVVFDRETRPREDVTVKATDRGDRPLIGFCQFSVEVVDINDNSPQFERPSYETSVSRFEAVGTSVITVFAFDNDAAHNAEITYSLEIDTTAGEEHQNDLDFFELVNRRSGEITLIKPIPMKTQKFIFNVIADDNGIPEALQSSAQVTLNVLDKQQKAPKWQTSPDCKPGITVDENVELNKVILRCRAVSSGDSRNSDVIYKLTASGGPGNKAESKFRQFNKFENGNEWVEVVIMEGLDYEQVNNYTLTLTATDMTSRVASTKTFVVEVRDVNDVVPQFTVDLFTGTIDEEMTPNEHLEKTNGKPIVTVKAIDTDSDGPQNEVHYRIVGEANGEETKHFRIDELTGEIFPNEKFDREKIDMYILTVEASDRSVSALPGANGPNKDNVKVQIVINDVNDNAPSFEEQKYIGRVKESEGEGHDVITIKAHDLDKHSNLRYHLIGAGGGRIPFGVRTDSGTIFVKEPLDFEASDQYHLVLIASDGRHNATTNVYIHIEDVNDNAPQFEQQKYATTVIEEDVDIPKVLFNVHATDADQDEKSSRIVYRLEGQGADEVFRIGKYSGTIELVKALDRDPPAGVPSWNFVVQAIDDDGNGLVGYADVQVNVRDINDNSPIFPERLFGYIEENREPIHSDGVYFMDVQARDFDDPTTENANIEYGIVRNKLINGESVFRIDQNTGKIFAMRSLDREISSEREFIIEVRANDRGVPSREGFANVTIKVTDMNDNAPFFEKTRYEGSVEETAPIGAAVMSFSAFDADEEAKDNVFTYQLSEESDYFYVTTDKDSKQSSVGVLRVKQPLDYEDVTQRDGFHLGIRVSDGRHDAEAAVHVALVDRNDHAPHIHGATEHRVREDVPRGTSIGRYTATDRDAGDTARFRINRQSDPKRQFTIDQDGTLRVAHTLDREDIAVYNLIIEAYDNSNNIGRQMVAVYLQDVNDNGPEPYTVPRPCIFRENTPVNQLGTCEIRATDRDTAEFGPPFTMEVSPSFKYSQYLNVIFNANGDGGNGSMTITPLQEFDREAPVPGKILEIPLILADRAGRRNEASVHVIIGDLNDNTMHDGRMTIHVNSYLGRLKETVIGRVYVDDADDWDLGDKTFSWKDSRPGFELSDKGSITMAGEMAAGTYTMSANVHDNARDEDAVGYVTVIVNAVPQIAFDNQGSVQLLIAEETPLQLPDDFIRADSNGQSLMDTFKQEMTAYMGGDVTVDVFSVQVGIATLQTRDVPVLNVRFNARGSTYRDTAQLNGLIAAHRADLQRKLNVEIVGVGIDMCKFTQCDAGCQTLNSADYDGIVVSANSTVIVGVNATSRDDCTCPVWRAPPACQHSLCHNDGVCHNTNPGFFCECRNDGLKGARCQGTTRSFGGNGFAWYKPMPACTSLNISFSFMTTQSDALLFYNGPLETLRNDTHIEYSDYIFIQLRGGRISLEVSMNGQSRSSLEVASTALNDGTWHDISVNQEGKRVELVVDNCRFLGAGADDSSCRAELYTPDDDERLNIVTPVQIGGLAPLSGQDYPQTIPRAGLNGCVRNLNVNGDQYDLATPAFEQNSEKGCRLWGATCDSNSVDSLNHCIHGDCFADVQGSGAMVAKCVCDPGWGGARCERRMEWIQFAQGAFIEYSPRIAFPEQVSDIELLFISGKVNGAPAELSFGTDSQQSYVSTNLESGQNGVTAAGKFDIGTGGRRARQELRVSEVLLKENASYWLQFTRNPTRASLSIDNAYTVSTQLDKGEPFSLQVNQITLGTQGQNKGFQGCIGTYRWSKQNLPLKRGGAMDENEESIVSISNMAGVQDGCDLRITCADLPAGYCGGSFVCVDFWKGPFCTCNDGANAILGDDGQVVGCGETLAVSKLGISSPAIILILVSLALLILLVMMMVVYTRRSPGAFENVRPEEMNRDNLRQYGVEGGGEADNDQYSMAGLRKPVMPLDTGMGPAIGGHPPHYPPRGMAPPKDDHELNSKIKDLETDQNAAPYDELRIYDDERDNISVVTLESIESAQ DIP protein Q967F4 original sequence version: 118 5E0D4C8FD24694FB axlp1_caeel Axin-like protein 1 K02B12.4 axl-1 protein protein caeel Caenorhabditis elegans MTMRSKFSIDRVLHDTAFAKWCQKEESRNADSITLYTSILDFESKLKTGSPSLSLLKLARHIHRKYVSLNTGTCHVIGDSIRTKISTRVHEVLDGKPPYIDLFDPLKQPLFQHLRSMHTEFSTTTADVNTTWEDASSTSSSNKGATIWFNEDAIDKSSRHEIGQSTVTHESEDDRFSFFNAVCTRLNSLQETKNSSETEEHAESPRKEKSSTPYGTDGFAPPPRSTQTNTLKVSNLPKRFESLYKKKRQQVATSDSSGFGSNASDFWSFERYGKSNQGTLERPNRLFTGTNNGFSTLQPKRRGSEAPKMTVELRYENDVPMVAKISANHAQSVTLRYFRHLFGLHYTDNCRFFFKSTCEDGSAQYQWTLLFHDDDILPVFQNRITAICRMCPPPEDHDLI 7C1E5CADC18D3B26 pry_1-axl_1 1 11 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey physical association physical association bar_1-axl_1 2 5 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey physical association physical association Fig 2 bar_1-pry_1-3 2 5 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association Fig 2 gsk_3-pry_1-1 2 11 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association Fig 2 gsk_3-axl_1 2 4 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey physical association physical association Fig 2 mig_5-pry_1-2 2 11 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 2 mig_5-axl_1-1 2 14 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association dsh_2-axl_1 2 14 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association apr_1-pry_1-1 2 11 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association hmp_2-hmr_1-1 2 7 unspecified role unspecified role bait bait re sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 13 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association axin1-gsk_3 3 12 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O15169:p.Gly521Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain G P Wed May 02 14:35:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly521pro' Tue Jun 30 15:56:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly521Pro' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey 35s-met 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction Fig 3A The mutation of human Axin is stated to be L521P, but no leucine is currently present at residue 521, nor in the previous version of the sequence. The mutation is most likely to be at L392P within the GSK3B binding region, as also found in the mouse axin protein, O35625. (Bax et al,. Structure, Vol 9, 2001. p1143-1152 ) Dear Checker The editor is very unstable at present so it is difficult to add/alter anything. I have added a annotation at FEATURE level for the caution below as an “invalid positions” - no idea if this is a correct use of the term, but was the only appropriate one present...please remove if you think it should not be there... axl_1-gsk_3-2 3 14 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role prey prey 35s-met 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction Fig 3A pry_1-gsk_3-1 3 4 unspecified role unspecified role prey prey 35s-met 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 11 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction Fig 3A axl_1-gsk3b 3 14 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey 35s-met 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction Fig 3B FRATtide:a peptide corresponding to residues 188 - 226 of human FRAT1. pry_1-gsk3b 3 8 unspecified role unspecified role prey prey 35s-met 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 11 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction Fig 3B FRATtide:a peptide corresponding to residues 188 - 226 of human FRAT1. axin1-gsk3b 3 8 unspecified role unspecified role prey prey 35s-met 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 12 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction Fig 3B FRATtide:a peptide corresponding to residues 188 - 226 of human FRAT1.