IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ PRAK is essential for ras-induced senescence and tumor suppression. PRAK is essential for ras-induced senescence and tumor suppression. Cell (0092-8674) 2007 imex curation Sun P., Yoshizuka N., New L., Moser BA., Li Y., Liao R., Xie C., Chen J., Deng Q., Yamout M., Dong MQ., Frangou CG., Yates JR., Wright PE., Han J. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer revisit when participant detection method molecular weight by autoradiography is available Note that the cell type expressed in was searched for in the cited references where necessary. pqsun@scripps.edu,jhan@scripps.edu 02-MAR-2006: Contacted by JYOTI. Only protein-protein interactions 7 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Sun P., Yoshizuka N., New L., Moser BA., Li Y., Liao R., Xie C., Chen J., Deng Q., Yamout M., Dong MQ., Frangou CG., Yates JR., Wright PE., Han J. Apoptosis - Interactions involving proteins with a function related to apoptosis revisit when participant detection method molecular weight by autoradiography is available revisit when participant detection method molecular weight by autoradiography is available revisit when participant detection method molecular weight by autoradiography is available Note that the cell type expressed in was searched for in the cited references where necessary. pqsun@scripps.edu,jhan@scripps.edu pqsun@scripps.edu,jhan@scripps.edu Accepted 2007-FEB-22 by DAVET. 02-MAR-2006: Contacted by JYOTI. Cell (0092-8674) 2007 Only protein-protein interactions 7 imex curation hspb1_human Heat shock protein beta-1 HSPB1 HSP27 HSP28 Heat shock 27 kDa protein Stress-responsive protein 27 Estrogen-regulated 24 kDa protein 28 kDa heat shock protein protein protein human Homo sapiens Human MTERRVPFSLLRGPSWDPFRDWYPHSRLFDQAFGLPRLPEEWSQWLGGSSWPGYVRPLPPAAIESPAVAAPAYSRALSRQLSSGVSEIRHTADRWRVSLDVNHFAPDELTVKTKDGVVEITGKHEERQDEHGYISRCFTRKYTLPPGVDPTQVSSSLSPEGTLTVEAPMPKLATQSNEITIPVTFESRAQLGGPEAAKSDETAAK 1B4DC44A6F6606D5 1B4DC44A6F6606D5 p53_mouse Cellular tumor antigen p53 Tp53 P53 Trp53 Tumor suppressor p53 protein protein mouse Mus musculus Mouse MTAMEESQSDISLELPLSQETFSGLWKLLPPEDILPSPHCMDDLLLPQDVEEFFEGPSEALRVSGAPAAQDPVTETPGPVAPAPATPWPLSSFVPSQKTYQGNYGFHLGFLQSGTAKSVMCTYSPPLNKLFCQLAKTCPVQLWVSATPPAGSRVRAMAIYKKSQHMTEVVRRCPHHERCSDGDGLAPPQHLIRVEGNLYPEYLEDRQTFRHSVVVPYEPPEAGSEYTTIHYKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRRTEEENFRKKEVLCPELPPGSAKRALPTCTSASPPQKKKPLDGEYFTLKIRGRKRFEMFRELNEALELKDAHATEESGDSRAHSSYLKTKKGQSTSRHKKTMVKKVGPDSD DIP protein P02340 original sequence version: 117 DIP protein P02340 original sequence version: 165 DIP protein P02340 original sequence version: 187 DIP protein P02340 original sequence version: 201 DIP protein P02340 original sequence version: 111 DIP protein P02340 original sequence version: 141 DIP protein P02340 original sequence version: 180 DIP protein P02340 original sequence version: 163 DIP protein P02340 original sequence version: 152 ED5A607378D921D7 mapk5_mouse MAP kinase-activated protein kinase 5 Mapkapk5 protein protein mouse Mus musculus Mouse MSEDSDMEKAIKETSILEEYSINWTQKLGAGISGPVRVCVKKSTQERFALKILLDRPKARNEVRLHMMCATHPNIVQIIEVFANSVQFPHESSPRARLLIVMEMMEGGELFHRISQHRHFTEKQASQVTKQIALALQHCHLLNIAHRDLKPENLLFKDNSLDAPVKLCDFGFAKVDQGDLMTPQFTPYYVAPQVLEAQRRHQKEKSGIIPTSPTPYTYNKSCDLWSLGVIIYVMLCGYPPFYSKHHSRTIPKDMRKKIMTGSFEFPEEEWSQISEMAKDVVRKLLKVKPEERLTIEGVLDHPWLNSTEALDNVLPSAQLMMDKAVVAGIQQAHAEQLANMRIQDLKVSLKPLHSVNNPILRKRKLLGTKPKDGIYIHDHENGTEDSNVALEKLRDVIAQCILPQAGKGENEDEKLNEVMQEAWKYNRECKLLRDALQSFSWNGRGFTDKVDRLKLAEVVKQVIEEQTLPHEPQ 45441A735075B247 atf2_human Cyclic AMP-dependent transcription factor ATF-2 ATF2 CREB2 CREBP1 Activating transcription factor 2 cAMP response element-binding protein CRE-BP1 HB16 Cyclic AMP-responsive element-binding protein 2 protein protein human Homo sapiens Human MKFKLHVNSARQYKDLWNMSDDKPFLCTAPGCGQRFTNEDHLAVHKHKHEMTLKFGPARNDSVIVADQTPTPTRFLKNCEEVGLFNELASPFENEFKKASEDDIKKMPLDLSPLATPIIRSKIEEPSVVETTHQDSPLPHPESTTSDEKEVPLAQTAQPTSAIVRPASLQVPNVLLTSSDSSVIIQQAVPSPTSSTVITQAPSSNRPIVPVPGPFPLLLHLPNGQTMPVAIPASITSSNVHVPAAVPLVRPVTMVPSVPGIPGPSSPQPVQSEAKMRLKAALTQQHPPVTNGDTVKGHGSGLVRTQSEESRPQSLQQPATSTTETPASPAHTTPQTQSTSGRRRRAANEDPDEKRRKFLERNRAAASRCRQKRKVWVQSLEKKAEDLSSLNGQLQSEVTLLRNEVAQLKQLLLAHKDCPVTAMQKKSGYHTADKDDSSEDISVPSSPHTEAIQHSSVSTSNGVSSTSKAEAVATSVLTQMADQSTEPALSQIVMAPSSQSQPSGS 0190EEFAEC8891A7 mapk5_human MAP kinase-activated protein kinase 5 MAPKAPK5 PRAK p38-regulated/activated protein kinase protein protein human Homo sapiens Human MSEESDMDKAIKETSILEEYSINWTQKLGAGISGPVRVCVKKSTQERFALKILLDRPKARNEVRLHMMCATHPNIVQIIEVFANSVQFPHESSPRARLLIVMEMMEGGELFHRISQHRHFTEKQASQVTKQIALALRHCHLLNIAHRDLKPENLLFKDNSLDAPVKLCDFGFAKIDQGDLMTPQFTPYYVAPQVLEAQRRHQKEKSGIIPTSPTPYTYNKSCDLWSLGVIIYVMLCGYPPFYSKHHSRTIPKDMRRKIMTGSFEFPEEEWSQISEMAKDVVRKLLKVKPEERLTIEGVLDHPWLNSTEALDNVLPSAQLMMDKAVVAGIQQAHAEQLANMRIQDLKVSLKPLHSVNNPILRKRKLLGTKPKDSVYIHDHENGAEDSNVALEKLRDVIAQCILPQAGKGENEDEKLNEVMQEAWKYNRECKLLRDTLQSFSWNGRGFTDKVDRLKLAEIVKQVIEEQTTSHESQ F3D9DDC83CC0C49D p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD DIP protein P04637 original sequence version: 121 DIP protein P04637 original sequence version: 115 DIP protein P04637-1 original sequence version: 224 DIP protein P04637 original sequence version: 117 DIP protein P04637-1 original sequence version: 226 DIP protein P04637 original sequence version: 226 DIP protein P04637 original sequence version: 212 DIP protein P04637 original sequence version: 220 DIP protein P04637-1 original sequence version: 220 DIP protein P04637-1 original sequence version: 145 DIP protein P04637 original sequence version: 146 DIP protein P04637 original sequence version: 145 DIP protein P04637 original sequence version: 166 DIP protein P04637-1 original sequence version: 166 DIP protein P04637 original sequence version: 169 DIP protein P04637 original sequence version: 177 DIP protein P04637 original sequence version: 175 DIP protein P04637-1 original sequence version: 175 DIP protein P04637-1 original sequence version: 172 DIP protein P04637-1 original sequence version: 169 DIP protein P04637 original sequence version: 170 DIP protein P04637 original sequence version: 172 DIP protein P04637-1 original sequence version: 194 DIP protein P04637 original sequence version: 196 DIP protein P04637 original sequence version: 185 DIP protein P04637-1 original sequence version: 191 DIP protein P04637 original sequence version: 191 DIP protein P04637 original sequence version: 180 DIP protein P04637-1 original sequence version: 197 DIP protein P04637 original sequence version: 183 DIP protein P04637-1 original sequence version: 183 DIP protein P04637-1 original sequence version: 185 DIP protein P04637 original sequence version: 199 DIP protein P04637 original sequence version: 201 DIP protein P04637 original sequence version: 189 DIP protein P04637 original sequence version: 235 DIP protein P04637 original sequence version: 230 DIP protein P04637 original sequence version: 234 DIP protein P04637-1 original sequence version: 234 DIP protein P04637-1 original sequence version: 235 DIP protein P04637 original sequence version: 239 DIP protein P04637-1 original sequence version: 212 DIP protein P04637-1 original sequence version: 213 DIP protein P04637 original sequence version: 213 DIP protein P04637 original sequence version: 206 DIP protein P04637-1 original sequence version: 188 DIP protein P04637-1 original sequence version: 228 DIP protein P04637 original sequence version: 208 DIP protein P04637 original sequence version: 240 DIP protein P04637-1 original sequence version: 240 DIP protein P04637 original sequence version: 204 DIP protein P04637-1 original sequence version: 204 DIP protein P04637-1 original sequence version: 209 DIP protein P04637-1 original sequence version: 203 DIP protein P04637 original sequence version: 221 DIP protein P04637-1 original sequence version: 152 DIP protein P04637 original sequence version: 217 DIP protein P04637-1 original sequence version: 140 DIP protein P04637 original sequence version: 215 DIP protein P04637 original sequence version: 214 DIP protein P04637-1 original sequence version: 182 DIP protein P04637 original sequence version: 182 DIP protein P04637 original sequence version: 188 DIP protein P04637 original sequence version: 192 DIP protein P04637-1 original sequence version: 192 DIP protein P04637 original sequence version: 218 DIP protein P04637-1 original sequence version: 218 AD5C149FD8106131 mk11_human Mitogen-activated protein kinase 11 MAPK11 PRKM11 SAPK2 Mitogen-activated protein kinase p38 beta p38-2 Stress-activated protein kinase 2b SAPK2B protein protein human Homo sapiens Human MSGPRAGFYRQELNKTVWEVPQRLQGLRPVGSGAYGSVCSAYDARLRQKVAVKKLSRPFQSLIHARRTYRELRLLKHLKHENVIGLLDVFTPATSIEDFSEVYLVTTLMGADLNNIVKCQALSDEHVQFLVYQLLRGLKYIHSAGIIHRDLKPSNVAVNEDCELRILDFGLARQADEEMTGYVATRWYRAPEIMLNWMHYNQTVDIWSVGCIMAELLQGKALFPGSDYIDQLKRIMEVVGTPSPEVLAKISSEHARTYIQSLPPMPQKDLSSIFRGANPLAIDLLGRMLVLDSDQRVSAAEALAHAYFSQYHDPEDEPEAEPYDESVEAKERTLEEWKELTYQEVLSFKPPEPPKPPGSLEIEQ 68DA4C7B7C721475 mapkapk5-tp53 1 7 enzyme target enzyme target substrate substrate unspecified role unspecified role n-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P04637:p.Ser37Asp mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S D Wed May 02 19:22:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser37asp' Tue Jun 30 19:41:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser37Asp' ecoli Escherichia coli (strain K12) 6 enzyme enzyme unspecified role unspecified role region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q8IW41:p.Thr182Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T A Wed May 02 15:23:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr182ala' Tue Jun 30 18:11:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr182Ala' Q8IW41:p.Lys51Met mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K M Wed May 02 16:08:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys51met' Tue Jun 30 17:09:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys51Met' human-bj Homo sapiens BJ normal foreskin fibroblasts bj Human normal foreskin fibroblasts The ATCC rference number is CRL-2522. The ATCC reference number is CRL-2522. phosphorylation phosphorylation reaction phosphorylation 7 A, B, C, D HA-tagged PRAK immunoprecipitated from BJ cells transduced with Ha-RasV12, MKK3E, or MKK6E readily phosphorylated human p53 in vitro HA-PRAK immunoprecipitated from BJ cells transduced with Ha-RasV12, MKK3E or MKK6E had higher levels of kinase activity than when the BJ cells were only transduced with vector. P04637 Ser37 is phosphorylated by PRAK. Phosphopeptide mapping by mass spectrometry confirmed Ser37 was the only residue phosphorylated by PRAK. The BJ cells from which PRAK was immunoprecipitated were senescent. mapkapk5-tp53-1 1 7 enzyme target enzyme target substrate substrate unspecified role unspecified role P04637:p.Ser37Asp mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S D Wed May 02 16:25:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser37asp' Tue Jun 30 18:43:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser37Asp' n-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobb Escherichia coli (strain B / BL21) phosphorylation phosphorylation reaction phosphorylation 7 E mutation of Ser37 to Asp essentially abolished phosphorylation by recombinant PRAK or PRAK immunoprecipitated from senescent cells, while mutations of Ser15 or Ser33 had no effect on p53 phosphorylation P04637 Ser37 is phosphorylated by PRAK. Phosphopeptide mapping by mass spectrometry confirmed Ser37 was the only residue phosphorylated by PRAK. mapk11-tp53 1 8 enzyme enzyme unspecified role unspecified role 7 enzyme target enzyme target substrate substrate unspecified role unspecified role n-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P04637:p.Ser33Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S D Wed May 02 18:09:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser33asp' Tue Jun 30 18:41:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser33Asp' ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation S10 B HA-PRAK immunoprecipitated from BJ cells transduced with Ha-RasV12, MKK3E or MKK6E had higher levels of kinase activity than when the BJ cells were only transduced with vector. P04637 Ser33 is phosphorylated by p38. The S37D protein was accessible to kinases since it was readily phosphorylated by recombinant and immunoprecipitated p38 at Ser33 ENZYME protein AF001008 mapkapk5-tp53-2 1 3 enzyme target enzyme target substrate substrate unspecified role unspecified role P02340:p.Ser37Asp mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S D Wed May 02 15:30:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser34asp' Tue Oct 30 14:50:47 GMT 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser34Asp' Tue Jun 30 19:46:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser37Asp' 4-60 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain The range was 1-57 but UniProt updated sequence so now range is 4-60. ecoli Escherichia coli (strain K12) 4 enzyme enzyme unspecified role unspecified role mouse-skin Mus musculus skin cells skin phosphorylation phosphorylation reaction phosphorylation PRAK immunoprecipitated from the wild-type papilloma had an increased kinase activity toward Ser37 of mp53 when compared to PRAK from the adjacent normal skin 7 I The PRAK immunoprecipitated from wild-type papilloma had increased kinase activity as compared to PRAK from adjacent normal skin. This suggests p53 Ser37 is phosphorylated by activated PRAK in tumours with senescent features. mapk5-hspb1 1 2 enzyme target enzyme target substrate substrate unspecified role unspecified role ecobb Escherichia coli (strain B / BL21) 4 enzyme enzyme unspecified role unspecified role mouse-skin Mus musculus skin cells skin phosphorylation phosphorylation reaction phosphorylation 5 C PRAK and p38 immunoprecipitated from the DMBA-induced papilloma in the wild-type mouse also showed increased kinase activity toward their substrates, HSP27 and ATF2 respectively, in comparison to the proteins isolated from normal skin in the same mouse Phosphorylation of HSPB1 PRAK from papilloma had increased kinase activity as compared with PRAK isolated from normal skin in the same mouse. mapk11-atf2 1 8 enzyme enzyme unspecified role unspecified role human-bj Homo sapiens BJ normal foreskin fibroblasts bj Human normal foreskin fibroblasts The ATCC rference number is CRL-2522. The ATCC reference number is CRL-2522. 5 enzyme target enzyme target substrate substrate unspecified role unspecified role ecobb Escherichia coli (strain B / BL21) phosphorylation phosphorylation reaction phosphorylation S10 A p38 immunoprecipitated from BJ cells at PD29 on day 7 post transduction with Ha-RasV12 or vector control was assayed for kinase activity toward ATF2, wild type p53(1-61) or indicated mutants of p53(1-61). Phosphorylation of ATF2 Transduction of BJ cells with Ha-RasV12 increased p38 kinase activity as compared to p38 isolated from cells transduced with the vector control. mapk11-tp53-1 1 7 enzyme target enzyme target substrate substrate unspecified role unspecified role n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P04637:p.Ser33Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S D Wed May 02 16:53:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser33asp' Tue Jun 30 17:13:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser33Asp' 8 enzyme enzyme unspecified role unspecified role human-bj Homo sapiens BJ normal foreskin fibroblasts bj Human normal foreskin fibroblasts The ATCC rference number is CRL-2522. The ATCC reference number is CRL-2522. phosphorylation phosphorylation reaction phosphorylation S10 A p38 immunoprecipitated from BJ cells at PD29 on day 7 post transduction with Ha-RasV12 or vector control was assayed for kinase activity toward ATF2, wild type p53(1-61) or indicated mutants of p53(1-61). Phosphorylation of TP53 Transduction of BJ cells with Ha-RasV12 increased p38 kinase activity as compared to p38 isolated from cells transduced with the vector control. The S37D protein was accessible to kinases since it was readily phosphorylated by recombinant and immunoprecipitated p38 at Ser33.