UniProt UniProt http://www.uniprot.org Protrudin induces neurite formation by directional membrane trafficking. Protrudin induces neurite formation by directional membrane trafficking. Science (0036-8075) 2006 mimix curation Shirane M., Nakayama KI. Apoptosis - Interactions involving proteins with a function related to apoptosis nakayak1@bioreg.kyushu-u.ac.jp 06-Jun-2011: Contacted by JYOTI. Human brain cDNA orchard rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells confocal microscopy confocal microscopy anti tag immunost anti tag immunostaining anti tag immunost Shirane M., Nakayama KI. Apoptosis - Interactions involving proteins with a function related to apoptosis nakayak1@bioreg.kyushu-u.ac.jp Accepted 2011-MAY-09 by ORCHARD 06-Jun-2011: Contacted by JYOTI. Human brain cDNA Science (0036-8075) 2006 orchard mimix curation Protrudin induces neurite formation by directional membrane trafficking. Protrudin induces neurite formation by directional membrane trafficking. Science (0036-8075) 2006 mimix curation Shirane M., Nakayama KI. Apoptosis - Interactions involving proteins with a function related to apoptosis nakayak1@bioreg.kyushu-u.ac.jp 06-Jun-2011: Contacted by JYOTI. Human brain cDNA orchard human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Shirane M., Nakayama KI. Apoptosis - Interactions involving proteins with a function related to apoptosis nakayak1@bioreg.kyushu-u.ac.jp Accepted 2011-MAY-09 by ORCHARD 06-Jun-2011: Contacted by JYOTI. Human brain cDNA Science (0036-8075) 2006 orchard mimix curation Protrudin induces neurite formation by directional membrane trafficking. Protrudin induces neurite formation by directional membrane trafficking. Science (0036-8075) 2006 mimix curation Shirane M., Nakayama KI. Apoptosis - Interactions involving proteins with a function related to apoptosis nakayak1@bioreg.kyushu-u.ac.jp 06-Jun-2011: Contacted by JYOTI. Human brain cDNA orchard rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Shirane M., Nakayama KI. Apoptosis - Interactions involving proteins with a function related to apoptosis nakayak1@bioreg.kyushu-u.ac.jp Accepted 2011-MAY-09 by ORCHARD 06-Jun-2011: Contacted by JYOTI. Human brain cDNA Science (0036-8075) 2006 orchard mimix curation Protrudin induces neurite formation by directional membrane trafficking. Protrudin induces neurite formation by directional membrane trafficking. Science (0036-8075) 2006 mimix curation Shirane M., Nakayama KI. Apoptosis - Interactions involving proteins with a function related to apoptosis nakayak1@bioreg.kyushu-u.ac.jp 06-Jun-2011: Contacted by JYOTI. Human brain cDNA orchard in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Shirane M., Nakayama KI. Apoptosis - Interactions involving proteins with a function related to apoptosis nakayak1@bioreg.kyushu-u.ac.jp Accepted 2011-MAY-09 by ORCHARD 06-Jun-2011: Contacted by JYOTI. Human brain cDNA Science (0036-8075) 2006 orchard mimix curation Protrudin induces neurite formation by directional membrane trafficking. Protrudin induces neurite formation by directional membrane trafficking. Science (0036-8075) 2006 mimix curation Shirane M., Nakayama KI. Apoptosis - Interactions involving proteins with a function related to apoptosis nakayak1@bioreg.kyushu-u.ac.jp 06-Jun-2011: Contacted by JYOTI. Human brain cDNA orchard yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Shirane M., Nakayama KI. Apoptosis - Interactions involving proteins with a function related to apoptosis nakayak1@bioreg.kyushu-u.ac.jp Accepted 2011-MAY-09 by ORCHARD 06-Jun-2011: Contacted by JYOTI. Human brain cDNA Science (0036-8075) 2006 orchard mimix curation zfy27_human Protrudin Spastic paraplegia 33 protein SPG33 Zinc finger FYVE domain-containing protein 27 ZFYVE27 protein protein human Homo sapiens Human MQTSEREGSGPELSPSVMPEAPLESPPFPTKSPAFDLFNLVLSYKRLEIYLEPLKDAGDGVRYLLRWQMPLCSLLTCLGLNVLFLTLNEGAWYSVGALMISVPALLGYLQEVCRARLPDSELMRRKYHSVRQEDLQRGRLSRPEAVAEVKSFLIQLEAFLSRLCCTCEAAYRVLHWENPVVSSQFYGALLGTVCMLYLLPLCWVLTLLNSTLFLGNVEFFRVVSEYRASLQQRMNPKQEEHAFESPPPPDVGGKDGLMDSTPALTPTEDLTPGSVEEAEEAEPDEEFKDAIEETHLVVLEDDEGAPCPAEDELALQDNGFLSKNEVLRSKVSRLTERLRKRYPTNNFGNCTGCSATFSVLKKRRSCSNCGNSFCSRCCSFKVPKSSMGATAPEAQRETVFVCASCNQTLSK zfy27_rat Protrudin Zinc finger FYVE domain-containing protein 27 Zfyve27 protein protein rat Rattus norvegicus (Rat) Rat MQSSDRDLSGPEASPSVMPEVLSECSPAPTKSTAFDLFNLVLSYKRLEIYLEPLKDAGDGVRYLLRWQMPLCSLLTCLGLNILFLTLNEGAWYSVGALIISVPALLGYLQEVCRAQLPESELMRRKYHSVRQEDLQRVRLSRPEAVAEVKSFLIRLEAFLARLCYTCESAYRVLHWENPVVSSQFYGALLGMVCMLYLLPLCWVLALLNSTLFLGNGEFFRVVSEYRACLQRRMSPKQEECVCEGSALQDAGGRAVVLDSTPAPTPTEDLTPGSVEEAEEAEPDEEFKDAIEEDDEGTPCPAEDELTMQDNGFLSKNEVLRSKVSKLTERLRKRYPTNNFGNCAGCAATFSVLKKRRSCSNCGNSFCSRCCSFKVPKSSMGATAPEAQRETVFVCASCNQTLSK 6D63A2ECB90264FC rb11a_rat Ras-related protein Rab-11A Rab11a Rab11 24KG protein protein rat Rattus norvegicus (Rat) Rat MGTRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADSNIVIMLVGNKSDLRHLRAVPTDEARAFAEKNGLSFIETSALDSTNVEAAFQTILTEIYRIVSQKQMSDRRENDMSPSNNVVPIHVPPTTENKPKVQCCQNI DIP protein P62494 original sequence version: 68 76FC1E113A29B269 fkbp8_human Peptidyl-prolyl cis-trans isomerase FKBP8 FK506-binding protein 8 38 kDa FK506-binding protein FKBPR38 FKBP8 FKBP38 Rotamase protein protein human Homo sapiens Human MASCAEPSEPSAPLPAGVPPLEDFEVLDGVEDAEGEEEEEEEEEEEDDLSELPPLEDMGQPPAEEAEQPGALAREFLAAMEPEPAPAPAPEEWLDILGNGLLRKKTLVPGPPGSSRPVKGQVVTVHLQTSLENGTRVQEEPELVFTLGDCDVIQALDLSVPLMDVGETAMVTADSKYCYGPQGRSPYIPPHAALCLEVTLKTAVDGPDLEMLTGQERVALANRKRECGNAHYQRADFVLAANSYDLAIKAITSSAKVDMTFEEEAQLLQLKVKCLNNLAASQLKLDHYRAALRSCSLVLEHQPDNIKALFRKGKVLAQQGEYSEAIPILRAALKLEPSNKTIHAELSKLVKKHAAQRSTETALYRKMLGNPSRLPAKCPGKGAWSIPWKWLFGATAVALGGVALSVVIAARN DIP protein Q14318 original sequence version: 145 887C25ADE71EBA8D rfip2_human Rab11 family-interacting protein 2 NRip11 RAB11FIP2 KIAA0941 protein protein human Homo sapiens Human MMLSEQAQKWFPTHVQVTVLQAKDLKPKGKSGTNDTYTIIQLGKEKYSTSVAEKTLEPVWKEEASFELPGLLIQGSPEKYILFLIVMHRSLVGLDKFLGQVAINLNDIFEDKQRRKTEWFRLESKQGKRIKNRGEIKVNIQFMRNNMTASMFDLSMKDKTRSPFAKLKDKMKGRKNDGTFSDTSSAIIPSTHMPDANSEFSSGEIQMKSKPKKPFLLGPQRLSSAHSMSDLSGSHMSSEKLKAGTIGQTHLLGHQLDSFGTVPESGSLKSPHRRTLSFDTSKMNQPDSIVDEGELCFGRQNDPFTNVTASLPQKFATLPRKKNPFEESSETWDSSMNLFSKPIEIRKENKREKREKVSLFERVTGKKDSRRSDKLNNGGSDSPCDLKSPNAFSENRQDYFDYESTNPFTAKFRASNIMPSSSFHMSPTSNEDLRKIPDSNPFDATAGYRSLTYEEVLQELVKHKELLRRKDTHIRELEDYIDNLLVRVMEETPSILRVPYEPSRKAGKFSNS DIP protein Q7L804 original sequence version: 30 9EC2DAB0A585BCD3 rb11a_human Ras-related protein Rab-11A YL8 RAB11A RAB11 protein protein human Homo sapiens Human MGTRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADSNIVIMLVGNKSDLRHLRAVPTDEARAFAEKNGLSFIETSALDSTNVEAAFQTILTEIYRIVSQKQMSDRRENDMSPSNNVVPIHVPPTTENKPKVQCCQNI DIP protein P62491 original sequence version: 38 DIP protein P62491-1 original sequence version: 132 DIP protein P62491 original sequence version: 122 DIP protein P62491 original sequence version: 89 DIP protein P62491-1 original sequence version: 137 DIP protein P62491 original sequence version: 136 DIP protein P62491 original sequence version: 137 DIP protein P62491-1 original sequence version: 140 76FC1E113A29B269 fkbp8-zfy27 5 6 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association materials and methods rab11a-zfy27 1 6 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component colocalization colocalization fig S3 q6p7b7-rab11a 3 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association 3B zfy27-rab11a 2 6 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association 3C rab11a-rab11fip2 2 11 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association S4A rab11a-zfy27-1 2 6 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association S4A rab11fip2-rab11a 4 10 unspecified role unspecified role bait bait spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 11 unspecified role unspecified role prey prey human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association S4C zfy27-rab11a-1 4 6 unspecified role unspecified role bait bait spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 11 unspecified role unspecified role prey prey human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association S4C