IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Proteomic identification of glucocorticoid receptor interacting proteins. Proteomic identification of glucocorticoid receptor interacting proteins. Proteomics (1615-9853) 2006 imex curation Hedman E., Widen C., Asadi A., Dinnetz I., Schroder WP., Gustafsson JA., Wikstrom AC. Apoptosis - Interactions involving proteins with a function related to apoptosis A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 2), CAA49670 and Q91YP3 ( a mouse protein) Both ligand activated and non-ligand activated samples were used in this study. Liganded state of the glucocorticoid recepter (GR) was prepared by adding 20nM triamcinolone acetonide to the cytosol at 47C for 1 h. Activation was later accomplished by dilution and heat-activation at 25C for 30 min.Protein spots were collected from a total of ten gels. All proteins that matched fingerprint spectra with a MOWSE score above 90 were considered significant. Proteins were mapped by using sequence similarity search of protein sequences identified by given identifiers within the paper against the UniProt database using the MPsrch tool. A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 1) 1NLD_L (identifies a mouse protein), ATRTC, CAA49670, XP_227551 and AAH86531. erik.hedman@mednut.ki.se orchard Only protein-protein interactions 5 rat-htc Rat hepatoma HTC cells htc Rat hepatoma HTC cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hedman E., Widen C., Asadi A., Dinnetz I., Schroder WP., Gustafsson JA., Wikstrom AC. Apoptosis - Interactions involving proteins with a function related to apoptosis A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 2), CAA49670 and Q91YP3 ( a mouse protein) Both ligand activated and non-ligand activated samples were used in this study. Liganded state of the glucocorticoid recepter (GR) was prepared by adding 20nM triamcinolone acetonide to the cytosol at 47C for 1 h. Activation was later accomplished by dilution and heat-activation at 25C for 30 min.Protein spots were collected from a total of ten gels. All proteins that matched fingerprint spectra with a MOWSE score above 90 were considered significant. Proteins were mapped by using sequence similarity search of protein sequences identified by given identifiers within the paper against the UniProt database using the MPsrch tool. A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 1) 1NLD_L (identifies a mouse protein), ATRTC, CAA49670, XP_227551 and AAH86531. erik.hedman@mednut.ki.se Accepted 2007-MAR-28 by ORCHARD. Proteomics (1615-9853) 2006 orchard Only protein-protein interactions 5 imex curation Proteomic identification of glucocorticoid receptor interacting proteins. Proteomic identification of glucocorticoid receptor interacting proteins. Proteomics (1615-9853) 2006 imex curation Hedman E., Widen C., Asadi A., Dinnetz I., Schroder WP., Gustafsson JA., Wikstrom AC. Apoptosis - Interactions involving proteins with a function related to apoptosis A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 2), CAA49670 and Q91YP3 ( a mouse protein) Both ligand activated and non-ligand activated samples were used in this study. Liganded state of the glucocorticoid recepter (GR) was prepared by adding 20nM triamcinolone acetonide to the cytosol at 47C for 1 h. Activation was later accomplished by dilution and heat-activation at 25C for 30 min.Protein spots were collected from a total of ten gels. All proteins that matched fingerprint spectra with a MOWSE score above 90 were considered significant. Proteins were mapped by using sequence similarity search of protein sequences identified by given identifiers within the paper against the UniProt database using the MPsrch tool. A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 1) 1NLD_L (identifies a mouse protein), ATRTC, CAA49670, XP_227551 and AAH86531. erik.hedman@mednut.ki.se orchard Only protein-protein interactions 5 rat-liver rattus norvegicus liver Rat liver liver bn-page blue native page bn-page ms participant Identification by mass spectrometry ms participant Hedman E., Widen C., Asadi A., Dinnetz I., Schroder WP., Gustafsson JA., Wikstrom AC. Apoptosis - Interactions involving proteins with a function related to apoptosis A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 2), CAA49670 and Q91YP3 ( a mouse protein) Both ligand activated and non-ligand activated samples were used in this study. Liganded state of the glucocorticoid recepter (GR) was prepared by adding 20nM triamcinolone acetonide to the cytosol at 47C for 1 h. Activation was later accomplished by dilution and heat-activation at 25C for 30 min.Protein spots were collected from a total of ten gels. All proteins that matched fingerprint spectra with a MOWSE score above 90 were considered significant. Proteins were mapped by using sequence similarity search of protein sequences identified by given identifiers within the paper against the UniProt database using the MPsrch tool. A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 1) 1NLD_L (identifies a mouse protein), ATRTC, CAA49670, XP_227551 and AAH86531. erik.hedman@mednut.ki.se Accepted 2007-MAR-28 by ORCHARD. Proteomics (1615-9853) 2006 orchard Only protein-protein interactions 5 imex curation Proteomic identification of glucocorticoid receptor interacting proteins. Proteomic identification of glucocorticoid receptor interacting proteins. Proteomics (1615-9853) 2006 imex curation Hedman E., Widen C., Asadi A., Dinnetz I., Schroder WP., Gustafsson JA., Wikstrom AC. Apoptosis - Interactions involving proteins with a function related to apoptosis A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 2), CAA49670 and Q91YP3 ( a mouse protein) Both ligand activated and non-ligand activated samples were used in this study. Liganded state of the glucocorticoid recepter (GR) was prepared by adding 20nM triamcinolone acetonide to the cytosol at 47C for 1 h. Activation was later accomplished by dilution and heat-activation at 25C for 30 min.Protein spots were collected from a total of ten gels. All proteins that matched fingerprint spectra with a MOWSE score above 90 were considered significant. Proteins were mapped by using sequence similarity search of protein sequences identified by given identifiers within the paper against the UniProt database using the MPsrch tool. A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 1) 1NLD_L (identifies a mouse protein), ATRTC, CAA49670, XP_227551 and AAH86531. erik.hedman@mednut.ki.se orchard Only protein-protein interactions 5 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Hedman E., Widen C., Asadi A., Dinnetz I., Schroder WP., Gustafsson JA., Wikstrom AC. Apoptosis - Interactions involving proteins with a function related to apoptosis A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 2), CAA49670 and Q91YP3 ( a mouse protein) Both ligand activated and non-ligand activated samples were used in this study. Liganded state of the glucocorticoid recepter (GR) was prepared by adding 20nM triamcinolone acetonide to the cytosol at 47C for 1 h. Activation was later accomplished by dilution and heat-activation at 25C for 30 min.Protein spots were collected from a total of ten gels. All proteins that matched fingerprint spectra with a MOWSE score above 90 were considered significant. Proteins were mapped by using sequence similarity search of protein sequences identified by given identifiers within the paper against the UniProt database using the MPsrch tool. A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 1) 1NLD_L (identifies a mouse protein), ATRTC, CAA49670, XP_227551 and AAH86531. erik.hedman@mednut.ki.se Accepted 2007-MAR-28 by ORCHARD. Proteomics (1615-9853) 2006 orchard Only protein-protein interactions 5 imex curation Proteomic identification of glucocorticoid receptor interacting proteins. Proteomic identification of glucocorticoid receptor interacting proteins. Proteomics (1615-9853) 2006 imex curation Hedman E., Widen C., Asadi A., Dinnetz I., Schroder WP., Gustafsson JA., Wikstrom AC. Apoptosis - Interactions involving proteins with a function related to apoptosis A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 2), CAA49670 and Q91YP3 ( a mouse protein) Both ligand activated and non-ligand activated samples were used in this study. Liganded state of the glucocorticoid recepter (GR) was prepared by adding 20nM triamcinolone acetonide to the cytosol at 47C for 1 h. Activation was later accomplished by dilution and heat-activation at 25C for 30 min.Protein spots were collected from a total of ten gels. All proteins that matched fingerprint spectra with a MOWSE score above 90 were considered significant. Proteins were mapped by using sequence similarity search of protein sequences identified by given identifiers within the paper against the UniProt database using the MPsrch tool. A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 1) 1NLD_L (identifies a mouse protein), ATRTC, CAA49670, XP_227551 and AAH86531. erik.hedman@mednut.ki.se orchard Only protein-protein interactions 5 rat-liver rattus norvegicus liver Rat liver liver anti bait coip anti bait coimmunoprecipitation anti bait coip ms participant Identification by mass spectrometry ms participant Hedman E., Widen C., Asadi A., Dinnetz I., Schroder WP., Gustafsson JA., Wikstrom AC. Apoptosis - Interactions involving proteins with a function related to apoptosis A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 2), CAA49670 and Q91YP3 ( a mouse protein) Both ligand activated and non-ligand activated samples were used in this study. Liganded state of the glucocorticoid recepter (GR) was prepared by adding 20nM triamcinolone acetonide to the cytosol at 47C for 1 h. Activation was later accomplished by dilution and heat-activation at 25C for 30 min.Protein spots were collected from a total of ten gels. All proteins that matched fingerprint spectra with a MOWSE score above 90 were considered significant. Proteins were mapped by using sequence similarity search of protein sequences identified by given identifiers within the paper against the UniProt database using the MPsrch tool. A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 1) 1NLD_L (identifies a mouse protein), ATRTC, CAA49670, XP_227551 and AAH86531. erik.hedman@mednut.ki.se Accepted 2007-MAR-28 by ORCHARD. Proteomics (1615-9853) 2006 orchard Only protein-protein interactions 5 imex curation Proteomic identification of glucocorticoid receptor interacting proteins. Proteomic identification of glucocorticoid receptor interacting proteins. Proteomics (1615-9853) 2006 imex curation Hedman E., Widen C., Asadi A., Dinnetz I., Schroder WP., Gustafsson JA., Wikstrom AC. Apoptosis - Interactions involving proteins with a function related to apoptosis A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 2), CAA49670 and Q91YP3 ( a mouse protein) Both ligand activated and non-ligand activated samples were used in this study. Liganded state of the glucocorticoid recepter (GR) was prepared by adding 20nM triamcinolone acetonide to the cytosol at 47C for 1 h. Activation was later accomplished by dilution and heat-activation at 25C for 30 min.Protein spots were collected from a total of ten gels. All proteins that matched fingerprint spectra with a MOWSE score above 90 were considered significant. Proteins were mapped by using sequence similarity search of protein sequences identified by given identifiers within the paper against the UniProt database using the MPsrch tool. A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 1) 1NLD_L (identifies a mouse protein), ATRTC, CAA49670, XP_227551 and AAH86531. erik.hedman@mednut.ki.se orchard Only protein-protein interactions 5 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Hedman E., Widen C., Asadi A., Dinnetz I., Schroder WP., Gustafsson JA., Wikstrom AC. Apoptosis - Interactions involving proteins with a function related to apoptosis A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 2), CAA49670 and Q91YP3 ( a mouse protein) Both ligand activated and non-ligand activated samples were used in this study. Liganded state of the glucocorticoid recepter (GR) was prepared by adding 20nM triamcinolone acetonide to the cytosol at 47C for 1 h. Activation was later accomplished by dilution and heat-activation at 25C for 30 min.Protein spots were collected from a total of ten gels. All proteins that matched fingerprint spectra with a MOWSE score above 90 were considered significant. Proteins were mapped by using sequence similarity search of protein sequences identified by given identifiers within the paper against the UniProt database using the MPsrch tool. A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 1) 1NLD_L (identifies a mouse protein), ATRTC, CAA49670, XP_227551 and AAH86531. erik.hedman@mednut.ki.se Accepted 2007-MAR-28 by ORCHARD. Proteomics (1615-9853) 2006 orchard Only protein-protein interactions 5 imex curation albu_rat Albumin Alb protein protein rat Rattus norvegicus (Rat) Rat MKWVTFLLLLFISGSAFSRGVFRREAHKSEIAHRFKDLGEQHFKGLVLIAFSQYLQKCPYEEHIKLVQEVTDFAKTCVADENAENCDKSIHTLFGDKLCAIPKLRDNYGELADCCAKQEPERNECFLQHKDDNPNLPPFQRPEAEAMCTSFQENPTSFLGHYLHEVARRHPYFYAPELLYYAEKYNEVLTQCCTESDKAACLTPKLDAVKEKALVAAVRQRMKCSSMQRFGERAFKAWAVARMSQRFPNAEFAEITKLATDVTKINKECCHGDLLECADDRAELAKYMCENQATISSKLQACCDKPVLQKSQCLAEIEHDNIPADLPSIAADFVEDKEVCKNYAEAKDVFLGTFLYEYSRRHPDYSVSLLLRLAKKYEATLEKCCAEGDPPACYGTVLAEFQPLVEEPKNLVKTNCELYEKLGEYGFQNAVLVRYTQKAPQVSTPTLVEAARNLGRVGTKCCTLPEAQRLPCVEDYLSAILNRLCVLHEKTPVSEKVTKCCSGSLVERRPCFSALTVDETYVPKEFKAETFTFHSDICTLPDKEKQIKKQTALAELVKHKPKATEDQLKTVMGDFAQFVDKCCKAADKDNCFATEGPNLVARSKEALA 07475796EA94938C fabpl_rat Fatty acid-binding protein, liver Fabp1 Liver-type fatty acid-binding protein Squalene- and sterol-carrier protein Z-protein p14 Fatty acid-binding protein 1 protein protein rat Rattus norvegicus (Rat) Rat MNFSGKYQVQSQENFEPFMKAMGLPEDLIQKGKDIKGVSEIVHEGKKVKLTITYGSKVIHNEFTLGEECELETMTGEKVKAVVKMEGDNKMVTTFKGIKSVTEFNGDTITNTMTLGDIVYKRVSKRI 08C7F59A99FE0D3A dnja2_rat DnaJ homolog subfamily A member 2 RDJ2 Dnaja2 protein protein rat Rattus norvegicus (Rat) Rat MANVADTKLYDILGVPPGASENELKKAYRKLAKEYHPDKNPNAGDKFKEISFAYEVLSNPEKRELYDRYGEQGLREGSGGGGGMDDIFSHIFGGGLFGFMGNQSRSRNGRRRGEDMMHPLKVSLEDLYNGKTTKLQLSKNVLCSACSGQGGKSGAVQKCSACRGRGVRIMIRQLAPGMVQQMQSVCSDCNGEGEVINEKDRCKKCEGKKVIKEVKILEVHVDKGMKHGQRITFTGEADQAPGVEPGDIVLFVQEKEHEVFQRDGNDLHMTYKIGLVEALCGFQFTFKHLDARQIVVKYPPGKVIEPGCVRVVRGEGMPQYRNPFEKGDLYIKFDVQFPENNWINPDKLSELEDLLPSRPEVPNVIGETEEVELQEFDSTRGSGGGQRREAYNDSSDEESSSHHGPGVQCAHQ FAE60601B55C409F skp1_rat S-phase kinase-associated protein 1 Skp1 Skp1a S-phase kinase-associated protein 1A Cyclin-A/CDK2-associated protein p19 p19skp1 p19A protein protein rat Rattus norvegicus (Rat) Rat MPTIKLQSSDGEIFEVDVEIAKQSVTIKTMLEDLGMDDEGDDDPVPLPNVNAAILKKVIQWCTHHKDDPPPPEDDENKEKRTDDIPVWDQEFLKVDQGTLFELILAANYLDIKGLLDVTCKTVANMIKGKTPEEIRKTFNIKNDFTEEEEAQVRKENQWCEEK DIP protein Q6PEC4 original sequence version: 31 AE184C00BE6556DB fetua_rat Alpha-2-HS-glycoprotein Fetuin-A Glycoprotein PP63 59 kDa bone sialic acid-containing protein Ahsg Fetua protein protein rat Rattus norvegicus (Rat) Rat MKSLVLLLCFAQLWSCQSAPQGAGLGFRELACDDPETEHVALIAVDYLNKHLLQGFRQILNQIDKVKVWSRRPFGEVYELEIDTLETTCHALDPTPLANCSVRQQAEHAVEGDCDFHILKQDGQFRVLHAQCHSTPDSAEDVRKFCPRCPILIRFNDTNVVHTVKTALAAFNAQNNGTYFKLVEISRAQNVPFPVSTLVEFVIAATDCTGQEVTDPAKCNLLAEKQYGFCKATLIHRLGGEEVSVACKLFQTQPQPANANPAGPAPTVGQAAPVAPPAGPPESVVVGPVAVPLGLPDHRTHHDLRHAFSPVASVESASGEVLHSPKVGQPGDAGAAGPVAPLCPGRVRYFKI 43564F60F3C7C90A hs90b_rat Heat shock protein HSP 90-beta Heat shock 84 kDa Hsp90ab1 Hsp84 Hspcb protein protein rat Rattus norvegicus (Rat) Rat MPEEVHHGEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNASDALDKIRYESLTDPSKLDSGKELKIDIIPNPQERTLTLVDTGIGMTKADLINNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVAEKVVVITKHNDDEQYAWESSAGGSFTVRADHGEPIGRGTKVILHLKEDQTEYLEERRVKEVVKKHSQFIGYPITLYLEKEREKEISDDEAEEEKGEKEEEDKEDEEKPKIEDVGSDEEDDSGKDKKKKTKKIKEKYIDQEELNKTKPIWTRNPDDITQEEYGEFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFIPRRAPFDLFENKKKKNNIKLYVRRVFIMDSCDELIPEYLNFIRGVVDSEDLPLNISREMLQQSKILKVIRKNIVKKCLELFSELAEDKENYKKFYEAFSKNLKLGIHEDSTNRRRLSELLRYHTSQSGDEMTSLSEYVSRMKETQKSIYYITGESKEQVANSAFVERVRKRGFEVVYMTEPIDEYCVQQLKEFDGKSLVSVTKEGLELPEDEEEKKKMEESKAKFENLCKLMKEILDKKVEKVTISNRLVSSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMMAKKHLEINPDHPIVETLRQKAEADKNDKAVKDLVVLLFETALLSSGFSLEDPQTHSNRIYRMIKLGLGIDEDEVTAEEPSAAVPDEIPPLEGDEDASRMEEVD CE323E81CE173ECB lasp1_rat LIM and SH3 domain protein 1 Lasp1 protein protein rat Rattus norvegicus (Rat) Rat MNPNCARCGKIVYPTEKVNCLDKFWHKACFHCETCKMTLNMKNYKGYEKKPYCNAHYPKQSFTMVADTPENLRLKQQSELQSQVRYKEEFEKNKGKGFSVVADTPELQRIKKTQDQISNIKYHEEFEKSRMGPSGGEGIEPERREAQDSSSYRRPTEQQQPQPHHIPTSAPVYQQPQQQQVTPSYGGYKEPAAPVSIQRSAPGGGGKRYRAVYDYSAADEDEVSFQDGDTIVNVQQIDDGWMYGTVERTGDTGMLPANYVEAI 63D416C4FA8B0744 hprt_rat Hypoxanthine-guanine phosphoribosyltransferase Hprt1 Hprt protein protein rat Rattus norvegicus (Rat) Rat MSTLSPSVVISDDEPGYDLDLFCIPNHYAEDLEKVFIPHGLIMDRTERLARDVMKEMGGHHIVALCVLKGGYKFFADLLDYIKALNRNSDRSIPMTVDFIRLKSYCNDQSTGDIKVIGGDDLSTLTGKNVLIVEDIIDTGKTMQTLLSLVKQYSPKMVKVASLLVKRTSRSVGYRPDFVGFEIPDKFVVGYALDYNEHFRDLNHVCVISESGKAKYKA 2683FC1444FE0B5E mvp_rat Major vault protein Mvp protein protein rat Rattus norvegicus (Rat) Rat MATEEAIIRIPPYHYIHVLDQNSNVSRVEVGPKTYIRQDNERVLFAPVRMVTVPPRHYCIVANPVSRDTQSSVLFDITGQVRLRHADQEIRLAQDPFPLYPGEVLEKDITPLQVVLPNTALHLKALLDFEDKNGDKVMAGDEWLFEGPGTYIPQKEVEVVEIIQATVIKQNQALRLRARKECFDREGKGRVTGEEWLVRSVGAYLPAVFEEVLDLVDAVILTEKTALHLRALQNFRDLRGVLHRTGEEWLVTVQDTEAHVPDVYEEVLGVVPITTLGPRHYCVILDPMGPDGKNQLGQKRVVKGEKSFFLQPGERLERGIQDVYVLSEQQGLLLKALQPLEEGESEEKVSHQAGDCWLIRGPLEYVPSAKVEVVEERQAIPLDQNEGIYVQDVKTGKVRAVIGSTYMLTQDEVLWEKELPSGVEELLNLGHDPLADRGQKGTAKPLQPSAPRNKTRVVSYRVPHNAAVQVYDYRAKRARVVFGPELVTLDPEEQFTVLSLSAGRPKRPHARRALCLLLGPDFFTDVITIETADHARLQLQLAYNWHFELKNRNDPAEAAKLFSVPDFVGDACKAIASRVRGAVASVTFDDFHKNSARIIRMAVFGFEMSEDTGPDGTLLPKARDQAVFPQNGLVVSSVDVQSVEPVDQRTRDALQRSVQLAIEITTNSQEAAAKHEAQRLEQEARGRLERQKILDQSEAEKARKELLELEAMSMAVESTGNAKAEAESRAEAARIEGEGSVLQAKLKAQALAIETEAELERVKKVREMELIYARAQLELEVSKAQQLANVEAKKFKEMTEALGPGTIRDLAVAGPEMQVKLLQSLGLKSTLITDGSSPINLFSTAFGLLGLGSDGQPPAQK DIP protein Q62667 original sequence version: 48 E51604F295D49A93 eloc_rat Elongin-C Eloc Elongin 15 kDa subunit RNA polymerase II transcription factor SIII subunit C SIII p15 Stromal membrane-associated protein SMAP1B homolog Tceb1 Transcription elongation factor B polypeptide 1 protein protein rat Rattus norvegicus (Rat) Rat MDGEEKTYGGCEGPDAMYVKLISSDGHEFIVKREHALTSGTIKAMLSGPGQFAENETNEVNFREIPSHVLSKVCMYFTYKVRYTNSSTEIPEFPIAPEIALELLMAANFLDC 98D88696E883538B elob_rat Elongin-B Elob Elongin 18 kDa subunit RNA polymerase II transcription factor SIII subunit B SIII p18 Tceb2 Transcription elongation factor B polypeptide 2 protein protein rat Rattus norvegicus (Rat) Rat MDVFLMIRRHKTTIFTDAKESSTVFELKRIVEGILKRPPEEQRLYKDDQLLDDGKTLGECGFTSQTARPQAPATVGLAFRADDTFEALRIEPFSSPPELPDVMKPQDSGGSANEQAVQ BA702F24CEC0FC02 hba_rat Hemoglobin subunit alpha-1/2 Hba1 Hba-a1 Hemoglobin alpha-1/2 chain Alpha-1/2-globin protein protein rat Rattus norvegicus (Rat) Rat MVLSADDKTNIKNCWGKIGGHGGEYGEEALQRMFAAFPTTKTYFSHIDVSPGSAQVKAHGKKVADALAKAADHVEDLPGALSTLSDLHAHKLRVDPVNFKFLSHCLLVTLACHHPGDFTPAMHASLDKFLASVSTVLTSKYR DEF6857594C42A99 fibb_rat Fibrinogen beta chain Liver regeneration-related protein LRRG036/LRRG043/LRRG189 Fgb Ab1-181 Ab1-216 Ac1-581 protein protein rat Rattus norvegicus (Rat) Rat MRHLWLLLLSVSLVQTQAATTDSDKVDLSIARGHRPVDRRKEEPPSLRPAPPPISGGGYRARPAKVDAGQKKVERKPPDAGGCVHGDGDMGVLCPTGCELRQTLLNHERPIKNSIAELNSNINSVSETSSVTFQYLTLLKDMWKKKQAQVKDNENVINEYSSILEDQKLYIDETVNDNIPLNLRVLRSILEDLRSKIQKLESDISAQTEYCHTPCTVNCNIPVVSGKECEEIIRKGGETSEMYLIQPDTSSKPYRVYCDMKTENGGWTVIQNRQDGSVDFGRKWDPYKKGFGNIATNEDTKKYCGLPGEYWLGNDKISQLTRIGPTELLIEMEDWKGDKVKAHYGGFTVQTEANKYQVSVNKYKGTAGNALMEGASQLVGENRTMTIHNGMFFSTYDRDNDGWVTTDPRKQCSKEDGGGWWYNRCHAANPNGRYYWGGLYSWDMSKHGTDDGVVWMNWKGSWYSMRRMSMKIRPVFPQQ 810698D9A65D0DE7 bhmt1_rat Betaine--homocysteine S-methyltransferase 1 Bhmt protein protein rat Rattus norvegicus (Rat) Rat MAPIAGKKAKRGILERLNAGEVVIGDGGFVFALEKRGYVKAGPWTPEAAVEHPEAVRQLHREFLRAGSNVMQTFTFYASEDKLENRGNYVAEKISGQKVNEAACDIARQVADEGDALVAGGVSQTPSYLSCKSETEVKKIFHQQLEVFMKKNVDFLIAEYFEHVEEAVWAVEALKTSGKPIAATMCIGPEGDLHGVSPGECAVRLVKAGAAIVGVNCHFDPSTSLQTIKLMKEGLEAARLKAYLMSHALAYHTPDCGKQGFIDLPEFPFGLEPRVATRWDIQKYAREAYNLGVRYIGGCCGFEPYHIRAIAEELAPERGFLPPASEKHGSWGSGLDMHTKPWIRARARKEYWQNLRIASGRPYNPSMSKPDAWGVTKGAAELMQQKEATTEQQLRALFEKQKFKSAQ 36E1D04A8E425887 dnja1_rat DnaJ homolog subfamily A member 1 DnaJ-like protein 1 Dnaja1 Hsj2 Rdj1 Heat shock protein J2 protein protein rat Rattus norvegicus (Rat) Rat MVKETTYYDVLGVKPNATQEELKKAYRKLALKYHPDKNPNEGEKFKQISQAYEVLADSKKRELYDKGGEQAIKEGGAGGGFGSPMDIFDMFFGGGGRMQRERRGKNVVHQLSVTLEDLYNGATRKLALQKNVICDKCEGRGGKKGAVECCPNCRGTGMQIRIHQIGPGMVQQIQSVCMECQGHGERISPKDRCKSCNGRKIVREKKILEVHIDKGMKDGQKITFHGEGDQEPGLEPGDIIIVLDQKDHAVFTRRGEDLFMCMDIQLVEALCGFQKPISTLDNRTIVITSHPGQIVKHGDIKCVLNEGMPIYRRPYEKGRLIIEFKVNFPENGFLSPDKLSLLEKLLPERKEVEETDEMDQVELVDFDPNQERRRHYNGEAYEDDEHHPRGGVQCQTS 1783CE3D5C4CD558 pawr_rat PRKC apoptosis WT1 regulator protein Prostate apoptosis response 4 protein Transcriptional repressor Par-4-like protein PAWR Pawr Par4 protein protein rat Rattus norvegicus (Rat) Rat MATGGYRSSGSTTDFLEEWKAKREKMRAKQNPVGPGSSGGDPAAKSPAGPLAQTTAAGTSELNHGPAGAAAPAAPGPGALNCAHGSSALPRGAPGSRRPEDECPIAAGAAGAPASRGDEEEPDSAPEKGRSSGPSARKGKGQIEKRKLREKRRSTGVVNIPAAECLDEYEDDEAGQKERKREDAITQQNTIQNEAASLPDPGTSYLPQDPSRTVPGRYKSTISAPEEEILNRYPRTDRSGFSRHNRDTSAPANFASSSTLEKRIEDLEKEVLRERQENLRLTRLMQDKEEMIGKLKEEIDLLNRDLDDMEDENEQLKQENKTLLKVVGQLTR 2069B32DEFFF160F tbb2b_rat Tubulin beta-2B chain T beta-15 Tubb2b Tubb2a protein protein rat Rattus norvegicus (Rat) Rat MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATADEQGEFEEEEGEDEA 4DC3956EFF880746 home2_rat Homer protein homolog 2 Cupidin VASP/Ena-related gene up-regulated during seizure and LTP 2 Homer2 Vesl2 protein protein rat Rattus norvegicus (Rat) Rat MGEQPIFTTRAHVFQIDPSTKKNWVPASKQAVTVSYFYDVTRNSYRIISVDGAKVIINSTITPNMTFTKTSQKFGQWADSRANTVFGLGFSSEQQLTKFAEKFQEVREAARLARDKSQEKIETSSNHSQESGCETPSSTQASSVNGTDDEKASHASPADTHLKSENDKLKIALTQSAANVKKWEIELQTLRESNARLTTALQESAASVEQWKRQFSICRDENDRLRSKIEELEEQCGEINREKEKNTQLKRRIEELESEVREKEMELKDLRKQSEIIPQLMSECEYVSEKLEAAERDNQNLEDKVRSLKTDIEESKYRQRHLKGELKSFLEVLDGKIDDLHDFRRGLSKLGTDN 205FA6358F9D198E gcr_rat Glucocorticoid receptor Nuclear receptor subfamily 3 group C member 1 Nr3c1 Grl protein protein rat Rattus norvegicus (Rat) Rat MDSKESLAPPGRDEVPGSLLGQGRGSVMDFYKSLRGGATVKVSASSPSVAAASQADSKQQRILLDFSKGSTSNVQQRQQQQQQQQQQQQQQQQQQQPDLSKAVSLSMGLYMGETETKVMGNDLGYPQQGQLGLSSGETDFRLLEESIANLNRSTSVPENPKSSTSATGCATPTEKEFPKTHSDASSEQQNRKSQTGTNGGSVKLYPTDQSTFDLLKDLEFSAGSPSKDTNESPWRSDLLIDENLLSPLAGEDDPFLLEGNTNEDCKPLILPDTKPKIKDTGDTILSSPSSVALPQVKTEKDDFIELCTPGVIKQEKLGPVYCQASFSGTNIIGNKMSAISVHGVSTSGGQMYHYDMNTASLSQQQDQKPVFNVIPPIPVGSENWNRCQGSGEDSLTSLGALNFPGRSVFSNGYSSPGMRPDVSSPPSSSSAATGPPPKLCLVCSDEASGCHYGVLTCGSCKVFFKRAVEGQHNYLCAGRNDCIIDKIRRKNCPACRYRKCLQAGMNLEARKTKKKIKGIQQATAGVSQDTSENPNKTIVPAALPQLTPTLVSLLEVIEPEVLYAGYDSSVPDSAWRIMTTLNMLGGRQVIAAVKWAKAILGLRNLHLDDQMTLLQYSWMFLMAFALGWRSYRQSSGNLLCFAPDLIINEQRMSLPCMYDQCKHMLFVSSELQRLQVSYEEYLCMKTLLLLSSVPKEGLKSQELFDEIRMTYIKELGKAIVKREGNSSQNWQRFYQLTKLLDSMHEVVENLLTYCFQTFLDKTMSIEFPEMLAEIITNQIPKYSNGNIKKLLFHQK DIP protein P06536 original sequence version: 183 DIP protein P06536 original sequence version: 143 DIP protein P06536 original sequence version: 139 9C9DE0B1D6724845 ppp6_rat Serine/threonine-protein phosphatase 6 catalytic subunit Protein phosphatase V Ppp6c Ppv protein protein rat Rattus norvegicus (Rat) Rat MAPLDLDKYVEIARQCKYLPENDLKRLCDYVCDLLLEESNVQPVSTPVTVCGDIHGQFYDLCELFRTGGQVPDTNYIFMGDFVDRGYYSLETFTYLLALKAKWPDRITLLRGNHESRQITQVYGFYDECQTKYGNANAWRYCTKVFDMLTVAALIDEQILCVHGGLSPDIKTLDQIRTIERNQEIPHKGAFCDLVWSDPEDVDTWAISPRGAGWLFGAKVTNEFVHINNLKLICRAHQLVHEGYKFMFDEKLVTVWSAPNYCYRCGNIASIMVFKDVNTREPKLFRAVPDSERVIPPRTTTPYFL 662969DE1B2EF490 prdx1_rat Peroxiredoxin-1 Thioredoxin peroxidase 2 Thioredoxin-dependent peroxide reductase 2 Heme-binding 23 kDa protein HBP23 Prdx1 Tdpx2 Thioredoxin-dependent peroxiredoxin 1 protein protein rat Rattus norvegicus (Rat) Rat MSSGNAKIGHPAPSFKATAVMPDGQFKDISLSDYKGKYVVFFFYPLDFTFVCPTEIIAFSDRAEEFKKLNCQVIGASVDSHFCHLAWINTPKKQGGLGPMNIPLVSDPKRTIAQDYGVLKADEGISFRGLFIIDDKGILRQITINDLPVGRSVDEILRLVQAFQFTDKHGEVCPAGWKPGSDTIKPDVNKSKEYFSKQK BDF2D4ABA8A776DA tba1a_rat Tubulin alpha-1A chain Tuba1a Tuba1 Tubulin alpha-1 chain Alpha-tubulin 1 protein protein rat Rattus norvegicus (Rat) Rat MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGVDSVEGEGEEEGEEY 00F8429A4A10E5FE q7tqn4_rat nos2 rCG_48632 Rela RelA protein protein rat Rattus norvegicus (Rat) Rat MDDLFPLIFPSEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLTPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFNGPTEPRPPPRRIAVPSRGPTSVPKPAPQPYAFSTSLSTINFDEFSPMVLPPGQISNQALALAPSSAPVLAQTMVPSSAMVPSLAQPPAPVPVLAPGPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNNTDPGVFTDLASVDNSEFQQLLNQGVAMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPATLGTSGLPNGLSGDEDFSSIADMDFSALLSQISS DIP protein Q7TQN4 original sequence version: 73 2C6A8FD68F667A64 a16a1_rat Aldehyde dehydrogenase family 16 member A1 Aldh16a1 protein protein rat Rattus norvegicus (Rat) Rat MAATRIQPSTREIFTTLEYGPVPESHACALAWLDTHNRLLGHYVNGMWLKPEHRNPAPCQDPITGENLASCLQAEAEDISAAVEAARITFKTWSQLPGTARGQHLTRLAKVIQKHQRLLWTLESLVTGRAVREVRDGDIPLAQQLLHYHALQAHAQDSALADWEPLGVIGLILPTSFSFLDMMWRVCPALAMGCTVVALVPPSFPTPLLLAQLAGELGSFPGILNVVCGPVSLGPVLASQPGVQKVAFCGAVEEGRALRQTLAGRGATLGLALGTESLLLLTDSADVDSAVEGVVDAVWSDRSLGGLRLLIQESVWDEAMKRLQARMAQIRSGKGLDGAVDMGARGAAARDLAQSFVDEAQSQGGQVFQAGDMPSNSPFFSPTLVSGLPPAAPCAQAEVPWPVVMASPFRTVKEALALANGTPRGGSASVWSERLGQALELGYGLQVGTVWINAHGLRDPAVPTGGCKESGSSWHGGPDGLYEYLQLLGTPYRESFLCESLNYDTFGLAVSSSLPSGPETGPSPAPPYGLFVRGRFQSPGTQSSRPIKDSSGKVSSYVAEGGAKDIRGAVEAAHQAAPGWGAQSPRARASLLWALAAALERRKQVLAAQLERHGAAPTVAETEVELSVRRLQTWATRVQDQGQTLQVTGLRGPVLRLREPLGVLAVVCPDEWPMLAFVSLLAPALAHGNAVVLVPSGSCPLLALEACQDIAALFPAGLVNVVTGDRDHLTRCLALHQDVQAMWYFGSAQGSQFVEWASAGNLKPVWVNRDFPRAWDVEVQGSEQELSLHAARTKALWLPMGD 44CAC35754443929 gcr_human Glucocorticoid receptor Nuclear receptor subfamily 3 group C member 1 NR3C1 GRL protein protein human Homo sapiens Human MDSKESLTPGREENPSSVLAQERGDVMDFYKTLRGGATVKVSASSPSLAVASQSDSKQRRLLVDFPKGSVSNAQQPDLSKAVSLSMGLYMGETETKVMGNDLGFPQQGQISLSSGETDLKLLEESIANLNRSTSVPENPKSSASTAVSAAPTEKEFPKTHSDVSSEQQHLKGQTGTNGGNVKLYTTDQSTFDILQDLEFSSGSPGKETNESPWRSDLLIDENCLLSPLAGEDDSFLLEGNSNEDCKPLILPDTKPKIKDNGDLVLSSPSNVTLPQVKTEKEDFIELCTPGVIKQEKLGTVYCQASFPGANIIGNKMSAISVHGVSTSGGQMYHYDMNTASLSQQQDQKPIFNVIPPIPVGSENWNRCQGSGDDNLTSLGTLNFPGRTVFSNGYSSPSMRPDVSSPPSSSSTATTGPPPKLCLVCSDEASGCHYGVLTCGSCKVFFKRAVEGQHNYLCAGRNDCIIDKIRRKNCPACRYRKCLQAGMNLEARKTKKKIKGIQQATTGVSQETSENPGNKTIVPATLPQLTPTLVSLLEVIEPEVLYAGYDSSVPDSTWRIMTTLNMLGGRQVIAAVKWAKAIPGFRNLHLDDQMTLLQYSWMFLMAFALGWRSYRQSSANLLCFAPDLIINEQRMTLPCMYDQCKHMLYVSSELHRLQVSYEEYLCMKTLLLLSSVPKDGLKSQELFDEIRMTYIKELGKAIVKREGNSSQNWQRFYQLTKLLDSMHEVVENLLNYCFQTFLDKTMSIEFPEMLAEIITNQIPKYSNGNIKKLLFHQK C5C90C9A5DD16AAB mp2k2_rat Dual specificity mitogen-activated protein kinase kinase 2 ERK activator kinase 2 MAPK/ERK kinase 2 Map2k2 Mek2 Mkk2 Prkmk2 protein protein rat Rattus norvegicus (Rat) Rat MLARRKPVLPALTINPTIAEGPSPTSEGASEAHLVDLQKKLEELDLDEQQRKRLEAFLTQKAKVGELKDDDFERISELGAGNGGVVTKARHRPSGLIMARKLIHLEIKPAVRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKEAKRIPEDILGKVSIAVLRGLAYLREKHQIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVELAIGRYPIPPPDAKELEASFGRPVVDGADGEPHSVSPRPRPPGRPISGHGMDSRPAMAIFELLDYIVNEPPPKLPSGVFSSDFQEFVNKCLIKNPAERADLKLLTNHAFIKRSEGEDVDFAGWLCRTLRLKQPSTPTRTAV B4E6AB15DEAA82A4 ttpa_rat Alpha-tocopherol transfer protein Ttpa Tpp1 protein protein rat Rattus norvegicus (Rat) Rat MAEMRPGPVVGKQLNEQPDHSPLVQPGLAELRRRAQEEGVPETPQPLTDAFLLRFLRARDFDLDLAWRLMKNYYKWRAECPELSADLHPRSILGLLKAGYHGVLRSRDPTGSRVLIYRISYWDPKVFTAYDVFRVSLITSELIVQEVETQRNGVKAIFDLEGWQISHAFQITPSVAKKIAAVVTDSFPLKVRGIHLINEPVIFHAVFSMIKPFLTEKIKGRIHLHGNNYKSSLLQHFPDILPLEYGGNESSMEDICQEWTNFIMKSEDYLSSISETIQ 96F16E3F227F140C alb-dnaja2-gr-hgprt 4 14 unspecified role unspecified role prey prey 28 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 32 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait 25 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 26 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 23 unspecified role unspecified role prey prey 21 unspecified role unspecified role prey prey 29 unspecified role unspecified role prey prey 22 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 31 unspecified role unspecified role prey prey 19 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 27 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey association association Table 1. Table 2. Silver stained 2-D gels of GR interacting proteins. Immunopurified GR from (A) liganded/activated and (B) nonliganded/ non-activated rat liver were separated on 2-D gels (18 cm IPG strips 3�10 non-linear in the first dimension followed by a gradient slab gel, T = 5�20%, in the second dimension). The protein patterns from both states were reproduced on five independent 2-D gels. Identified proteins have been marked and are listed in Table 1. The following proteins were reported as having avarage ratios (non-liganded(nn-activated) over liganded (activated))of standardised spot volumes. P24090 (ratio = 5.84), Q6AYZ1(ratio = 4.17), P06399 (four spots ratio = 4.83, 3.88, 6.37,7.09 ), P63037 (ratio = 11.29), P14480 (4.28), P13832 (two spots ratio = -21.44, -13.40), P62870 (ratio = 5.95) and P27605 (ratio = -13.38) gr-mvp 3 30 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 2a Lane 3 shows that precipitated GST-MVP co-precipitates 35[S]-methionine labeled GR. gr-mvp-1 1 24 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey physical association physical association 2b MVP co-immunoprecipitates with GR. gr-mvp-2 5 30 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait physical association physical association 2c Transcriptional interaction between GR and MVP. gp-hgprt-skip1a 2 11 unspecified role unspecified role neutral component neutral component 29 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component 24 unspecified role unspecified role neutral component neutral component 19 unspecified role unspecified role neutral component neutral component physical association physical association Table 2 We identified the previously known GR interacting proteins hsp70 and hsp90 and others listed in Table 3. The authors provide a list of proteins isolated from several separate protein complexes and do not differentiate which proteins were identified in a specific complex. This entry lists the proteins from several protein complexes as set out in Table 2 of the report. Therefore the user should be aware that this entry does not indicate that all of the proteins were found in a single protein complex. The participant EBI-1187287(O09171) was duplicated. It is posssible that originally this interaction did contain more participants pointing to different Trembl proteins which have been merged to a single swissprot protein later.