IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Amyloid-beta peptide binds with heme to form a peroxidase: relationship to the cytopathologies of Alzheimer's disease. Amyloid-beta peptide binds with heme to form a peroxidase: relationship to the cytopathologies of Alzheimer's disease. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2006 imex curation Atamna H., Boyle K. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Apoptosis - Interactions involving proteins with a function related to apoptosis Alzheimers - Interactions investigated in the context of Alzheimers disease hatamna@chori.org 27-Oct-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel western blot western blot Immuno blot Immuno blot Atamna H., Boyle K. Apoptosis - Interactions involving proteins with a function related to apoptosis High molecular weight abeta aggregates were incubated with heme. After incubation smaller,SDS stable, aggregates were detected by western blotting with an antibody to abeta (6E10). The small SDS stable abeta aggregates are predicted to be products of reaggregation of abeta during sample prepartion following dismantling of the aggregates by heme treatment. hatamna@chori.org 27-Oct-2007: Contacted by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2006 Only protein-protein interactions imex curation Amyloid-beta peptide binds with heme to form a peroxidase: relationship to the cytopathologies of Alzheimer's disease. Amyloid-beta peptide binds with heme to form a peroxidase: relationship to the cytopathologies of Alzheimer's disease. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2006 imex curation Atamna H., Boyle K. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Apoptosis - Interactions involving proteins with a function related to apoptosis Alzheimers - Interactions investigated in the context of Alzheimers disease hatamna@chori.org 27-Oct-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro fluorescence fluorescence technology fluorescence predetermined predetermined participant predetermined Atamna H., Boyle K. Apoptosis - Interactions involving proteins with a function related to apoptosis The fluoresence of thioflavin-T (TfT) is high after binding abeta aggregates. When heme was added to abeta aggregates the flouresence of TfT declined, indicating a dismantling of abeta aggregates. hatamna@chori.org Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2006 Only protein-protein interactions imex curation Amyloid-beta peptide binds with heme to form a peroxidase: relationship to the cytopathologies of Alzheimer's disease. Amyloid-beta peptide binds with heme to form a peroxidase: relationship to the cytopathologies of Alzheimer's disease. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2006 imex curation Atamna H., Boyle K. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Apoptosis - Interactions involving proteins with a function related to apoptosis Alzheimers - Interactions investigated in the context of Alzheimers disease hatamna@chori.org 27-Oct-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Atamna H., Boyle K. Apoptosis - Interactions involving proteins with a function related to apoptosis hatamna@chori.org 27-Oct-2007: Contacted by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2006 Only protein-protein interactions imex curation p05067-pro_0000000092 Amyloid-beta protein 42 Alzheimer disease amyloid protein Cerebral vascular amyloid peptide APP A4 AD1 ABPP PreA4 Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 672 713 3AC85563D7858C37 hemin chlorido(protoporphyrinato)iron(III) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) hemin-p05067pro 3 5 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component physical association physical association 2. The identity of the heme group used was confirmed using the chemical formula from the suppliers web site. Ab exhibited peroxidase-like activity in the presence of heme. hemin-p05067pro-1 2 5 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association 1A and B The identity of the heme group used was confirmed using the chemical formula from the suppliers web site. Ab exhibited peroxidase-like activity in the presence of heme. p05067pro-1 1 4 unspecified role unspecified role neutral component neutral component physical association physical association 1C Positive control. Higher molecular weight oligomers also form. p05067pro 1 4 unspecified role unspecified role neutral component neutral component physical association physical association 1C Positive control. Higher molecular weight oligomers also form.