IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Immediate early gene X-1 interacts with proteins that modulate apoptosis. Immediate early gene X-1 interacts with proteins that modulate apoptosis. Biochem. Biophys. Res. Commun. (0006-291X) 2004 imex curation Kumar R., Lutz W., Frank E., Im HJ. Apoptosis - Interactions involving proteins with a function related to apoptosis rkumar@mayo.edu 14-May-2008: Contacted by JYOTI. A human kidney cDNA library was used. orchard Only protein-protein interactions human-hacat Homo sapiens immortalised keratinocytes hacat human immortalised keratinocytes anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kumar R., Lutz W., Frank E., Im HJ. Apoptosis - Interactions involving proteins with a function related to apoptosis rkumar@mayo.edu Accepted 2008-MAY-1 by ORCHARD. 14-May-2008: Contacted by JYOTI. Biochem. Biophys. Res. Commun. (0006-291X) 2004 orchard Only protein-protein interactions imex curation Immediate early gene X-1 interacts with proteins that modulate apoptosis. Immediate early gene X-1 interacts with proteins that modulate apoptosis. Biochem. Biophys. Res. Commun. (0006-291X) 2004 imex curation Kumar R., Lutz W., Frank E., Im HJ. Apoptosis - Interactions involving proteins with a function related to apoptosis rkumar@mayo.edu 14-May-2008: Contacted by JYOTI. A human kidney cDNA library was used. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Kumar R., Lutz W., Frank E., Im HJ. Apoptosis - Interactions involving proteins with a function related to apoptosis rkumar@mayo.edu Accepted 2008-MAY-1 by ORCHARD. 14-May-2008: Contacted by JYOTI. A human kidney cDNA library was used. Biochem. Biophys. Res. Commun. (0006-291X) 2004 orchard Only protein-protein interactions imex curation bag6_human Large proline-rich protein BAG6 BAT3 G3 HLA-B-associated transcript 3 Protein G3 BCL2-associated athanogene 6 BAG6 BAG family molecular chaperone regulator 6 Protein Scythe protein protein human Homo sapiens Human MEPNDSTSTAVEEPDSLEVLVKTLDSQTRTFIVGAQMNVKEFKEHIAASVSIPSEKQRLIYQGRVLQDDKKLQEYNVGGKVIHLVERAPPQTHLPSGASSGTGSASATHGGGSPPGTRGPGASVHDRNANSYVMVGTFNLPSDGSAVDVHINMEQAPIQSEPRVRLVMAQHMIRDIQTLLSRMETLPYLQCRGGPQPQHSQPPPQPPAVTPEPVALSSQTSEPVESEAPPREPMEAEEVEERAPAQNPELTPGPAPAGPTPAPETNAPNHPSPAEYVEVLQELQRLESRLQPFLQRYYEVLGAAATTDYNNNHEGREEDQRLINLVGESLRLLGNTFVALSDLRCNLACTPPRHLHVVRPMSHYTTPMVLQQAAIPIQINVGTTVTMTGNGTRPPPTPNAEAPPPGPGQASSVAPSSTNVESSAEGAPPPGPAPPPATSHPRVIRISHQSVEPVVMMHMNIQDSGTQPGGVPSAPTGPLGPPGHGQTLGQQVPGFPTAPTRVVIARPTPPQARPSHPGGPPVSGTLQGAGLGTNASLAQMVSGLVGQLLMQPVLVAQGTPGMAPPPAPATASASAGTTNTATTAGPAPGGPAQPPPTPQPSMADLQFSQLLGNLLGPAGPGAGGSGVASPTITVAMPGVPAFLQGMTDFLQATQTAPPPPPPPPPPPPAPEQQTMPPPGSPSGGAGSPGGLGLESLSPEFFTSVVQGVLSSLLGSLGARAGSSESIAAFIQRLSGSSNIFEPGADGALGFFGALLSLLCQNFSMVDVVMLLHGHFQPLQRLQPQLRSFFHQHYLGGQEPTPSNIRMATHTLITGLEEYVRESFSLVQVQPGVDIIRTNLEFLQEQFNSIAAHVLHCTDSGFGARLLELCNQGLFECLALNLHCLGGQQMELAAVINGRIRRMSRGVNPSLVSWLTTMMGLRLQVVLEHMPVGPDAILRYVRRVGDPPQPLPEEPMEVQGAERASPEPQRENASPAPGTTAEEAMSRGPPPAPEGGSRDEQDGASAETEPWAAAVPPEWVPIIQQDIQSQRKVKPQPPLSDAYLSGMPAKRRKTMQGEGPQLLLSEAVSRAAKAAGARPLTSPESLSRDLEAPEVQESYRQQLRSDIQKRLQEDPNYSPQRFPNAQRAFADDP 625B5F86321367ED tnf10_human Tumor necrosis factor ligand superfamily member 10 TNF-related apoptosis-inducing ligand Apo-2 ligand TNFSF10 APO2L TRAIL protein protein human Homo sapiens Human MAMMEVQGGPSLGQTCVLIVIFTVLLQSLCVAVTYVYFTNELKQMQDKYSKSGIACFLKEDDSYWDPNDEESMNSPCWQVKWQLRQLVRKMILRTSEETISTVQEKQQNISPLVRERGPQRVAAHITGTRGRSNTLSSPNSKNEKALGRKINSWESSRSGHSFLSNLHLRNGELVIHEKGFYYIYSQTYFRFQEEIKENTKNDKQMVQYIYKYTSYPDPILLMKSARNSCWSKDAEYGLYSIYQGGIFELKENDRIFVSVTNEHLIDMDHEASFFGAFLVG DIP protein P50591 original sequence version: 150 DDAAAF78DAAB2F6D q07820-2 Induced myeloid leukemia cell differentiation protein Mcl-1 Delta S MCL-1S TM MCL1 BCL2L3 Bcl-2-like protein 3 Bcl-2-related protein EAT/mcl1 mcl1/EAT protein protein human Homo sapiens Human MFGLKRNAVIGLNLYCGGAGLGAGSGGATRPGGRLLATEKEASARREIGGGEAGAVIGGSAGASPPSTLTPDSRRVARPPPIGAEVPDVTATPARLLFFAPTRRAAPLEEMEAPAADAIMSPEEELDGYEPEPLGKRPAVLPLLELVGESGNNTSTDGSLPSTPPPAEEEEDELYRQSLEIISRYLREQATGAKDTKPMGRSGATSRKALETLRRVGDGVQRNHETAFQGWVCGVLPCRGPRRWHQECAAGFCRCCWSRSWFGISNKIALL FAE0BF9E126523EF iex1_human Radiation-inducible immediate-early gene IEX-1 Immediate early protein GLY96 Immediate early response 3 protein IER3 DIF2 IEX1 PRG1 PACAP-responsive gene 1 protein Differentiation-dependent gene 2 protein protein protein human Homo sapiens Human MCHSRSCHPTMTILQAPTPAPSTIPGPRRGSGPEIFTFDPLPEPAAAPAGRPSASRGHRKRSRRVLYPRVVRRQLPVEEPNPAKRLLFLLLTIVFCQILMAEEGVPAPLPPEDAPNAASLAPTPVSAVLEPFNLTSEPSDYALDLSTFLQQHPAAF 83C06116C81B8341 camlg_human Guided entry of tail-anchored proteins factor CAMLG CAMLG CAML Calcium signal-modulating cyclophilin ligand GET2 protein protein human Homo sapiens Human MESMAVATDGGERPGVPAGSGLSASQRRAELRRRKLLMNSEQRINRIMGFHRPGSGAEEESQTKSKQQDSDKLNSLSVPSVSKRVVLGDSVSTGTTDQQGGVAEVKGTQLGDKLDSFIKPPECSSDVNLELRQRNRGDLTADSVQRGSRHGLEQYLSRFEEAMKLRKQLISEKPSQEDGNTTEEFDSFRIFRLVGCALLALGVRAFVCKYLSIFAPFLTLQLAYMGLYKYFPKSEKKIKTTVLTAALLLSGIPAEVINRSMDTYSKMGEVFTDLCVYFFTFIFCHELLDYWGSEVP 22DCD8CF022FEF09 iex1-camlg 2 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association using IEX-1 as the bait protein, and examined interactions between IEX-1 and proteins expressed by a human kidney cDNA expression library. We found that IEX-1 interacts with several proteins of which at least four are known to play a role in the regulation of apoptosis: (1) calcium-modulating cyclophilin ligand PREY gene NM_001745 iex1-tnfsf10 2 6 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association we performed yeast two-hybrid studies using IEX-1 as the bait protein, and examined interactions between IEX-1 and proteins expressed by a human kidney cDNA expression library. We found that IEX-1 interacts with several proteins of which at least four are known to play a role in the regulation of apoptosis: (1) calcium-modulating cyclophilin ligand; (2) tumor necrosis factor-related apoptosis-inducing ligand (tumor necrosis factor superfamily, member 10 PREY gene NM_003810 The paper states the identifier for TNFSF10 as NM_00381, whereas it is actually NM_003810. iex1-mcl1 2 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association PREY gene NM_182763 we performed yeast two-hybrid studies using IEX-1 as the bait protein, and examined interactions between IEX-1 and proteins expressed by a human kidney cDNA expression library. We found that IEX-1 interacts with several proteins of which at least four are known to play a role in the regulation of apoptosis: (1) calcium-modulating cyclophilin ligand; (2) tumor necrosis factor-related apoptosis-inducing ligand (tumor necrosis factor superfamily, member 10); (3) ML-1 myeloid cell leukemia gene encoded protein; iex1-bat3 2 6 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey physical association physical association PREY gene NM_080703 we performed yeast two-hybrid studies using IEX-1 as the bait protein, and examined interactions between IEX-1 and proteins expressed by a human kidney cDNA expression library. We found that IEX-1 interacts with several proteins of which at least four are known to play a role in the regulation of apoptosis: (1) calcium-modulating cyclophilin ligand; (2) tumor necrosis factor-related apoptosis-inducing ligand (tumor necrosis factor superfamily, member 10); (3) ML-1 myeloid cell leukemia gene encoded protein; and (4) BAT3 iex1-camlg-1 1 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association PREY gene NM_001745 CAML was immunoprecipitated with IEX-1 and was detected with an anti-CAML antibody.