IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Ring finger protein ZIN interacts with human immunodeficiency virus type 1 Vif. Ring finger protein ZIN interacts with human immunodeficiency virus type 1 Vif. J. Virol. (0022-538X) 2004 imex curation Feng F., Davis A., Lake JA., Carr J., Xia W., Burrell C., Li P. Apoptosis - Interactions involving proteins with a function related to apoptosis fig 3 peng.li@imvs.sa.gov.au Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Feng F., Davis A., Lake JA., Carr J., Xia W., Burrell C., Li P. Apoptosis - Interactions involving proteins with a function related to apoptosis fig 3 peng.li@imvs.sa.gov.au Annotation to indicate that this entry has been checked and accepted by a senior curator. J. Virol. (0022-538X) 2004 Only protein-protein interactions imex curation Ring finger protein ZIN interacts with human immunodeficiency virus type 1 Vif. Ring finger protein ZIN interacts with human immunodeficiency virus type 1 Vif. J. Virol. (0022-538X) 2004 imex curation Feng F., Davis A., Lake JA., Carr J., Xia W., Burrell C., Li P. Apoptosis - Interactions involving proteins with a function related to apoptosis fig 3 peng.li@imvs.sa.gov.au Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Feng F., Davis A., Lake JA., Carr J., Xia W., Burrell C., Li P. Apoptosis - Interactions involving proteins with a function related to apoptosis fig 3 peng.li@imvs.sa.gov.au Annotation to indicate that this entry has been checked and accepted by a senior curator. J. Virol. (0022-538X) 2004 Only protein-protein interactions imex curation q9nwf9-3 E3 ubiquitin-protein ligase RNF216 RNF216 TRIAD3 UBCE7IP1 ZIN RING finger protein 216 Triad domain-containing protein 3 Ubiquitin-conjugating enzyme 7-interacting protein 1 Zinc finger protein inhibiting NF-kappa-B ZIN TRIAD3 RING-type E3 ubiquitin transferase RNF216 protein protein human Homo sapiens Human MADFKVLSSQDIKWALHELKGHYAITRKALSDAIKKWQELSPETSGKRKKRKQMNQYSYIDFKFEQGDIKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKLELSCMEGSCTCSFPTSELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPHCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTGLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPVEKVQRVEALPRPVPQNLPQPQMPPYAFAHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNVRVNYDFGPIHMPLEHNLPMHFGPQPRHRF 4 different alternatively spliced mRNAs code for this protein isoform. 6EB1D218DE2E4E6A vif_hv1n5 Virion infectivity factor SOR protein vif protein protein hv1n5 Human immunodeficiency virus type 1 group M subtype B (isolate NY5) (HIV-1) HIV-1 MENRWQVMIVWQVDRMRINTWKRLVKHHMYISRKAKDWFYRHHYESTNPKISSEVHIPLGDAKLVITTYWGLHTGERDWHLGQGVSIEWRKKRYSTQVDPDLADQLIHLHYFDCFSESAIRNTILGRIVSPRCEYQAGHNKVGSLQYLALAALIKPKQIKPPLPSVRKLTEDRWNKPQKTKGHRGSHTMNGH DIP protein P12504 original sequence version: 90 2830B3233E8ECD16 ubce7ip1-vif-1 1 4 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role prey prey n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association HIV-1 pNL4.3 corresponding to HIV M19921 (HV1N5) was used to amplify vif. ubce7ip1-vif-2 2 3 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association HIV-1 pNL4.3 corresponding to HIV M19921 (HV1N5) was used to amplify vif. ubce7ip1-vif-3 2 3 unspecified role unspecified role bait bait n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association HIV-1 pNL4.3 corresponding to HIV M19921 (HV1N5) was used to amplify vif.