IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Identification of ASK and clock-associated proteins as molecular partners of LKP2 (LOV kelch protein 2) in Arabidopsis. Identification of ASK and clock-associated proteins as molecular partners of LKP2 (LOV kelch protein 2) in Arabidopsis. J. Exp. Bot. (0022-0957) 2004 imex curation Yasuhara M., Mitsui S., Hirano H., Takanabe R., Tokioka Y., Ihara N., Komatsu A., Seki M., Shinozaki K., Kiyosue T. Apoptosis - Interactions involving proteins with a function related to apoptosis The authors refer to ASK20a and ASK20b proteins which arise from splice variants of ASK20. However these splice variants are not annotated within the Uniprot entry for the ASK20 protein. Any interactions between bait and ASK20a and/or ASK20b proteins are represented as bait interacting with ASK20 protein in this entry. It is not posible to determne the coordinates of LOV and kelch domains used by the authors , therefore some data could not be captured such as that given in Figs 6 and 8. All proteins were identified from references in the text or from the TAIR database (The Arabidopsis Information Resource database) website and mapped using the MPsrch seqeunce similarity search tool to UniProt. tkiyosue@ag.kagawa-u.ac.jp 22-Jan-2008: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Yasuhara M., Mitsui S., Hirano H., Takanabe R., Tokioka Y., Ihara N., Komatsu A., Seki M., Shinozaki K., Kiyosue T. Apoptosis - Interactions involving proteins with a function related to apoptosis The authors refer to ASK20a and ASK20b proteins which arise from splice variants of ASK20. However these splice variants are not annotated within the Uniprot entry for the ASK20 protein. Any interactions between bait and ASK20a and/or ASK20b proteins are represented as bait interacting with ASK20 protein in this entry. It is not posible to determne the coordinates of LOV and kelch domains used by the authors , therefore some data could not be captured such as that given in Figs 6 and 8. All proteins were identified from references in the text or from the TAIR database (The Arabidopsis Information Resource database) website and mapped using the MPsrch seqeunce similarity search tool to UniProt. tkiyosue@ag.kagawa-u.ac.jp Accepted 2007-DEC-14 by JYOTI. J. Exp. Bot. (0022-0957) 2004 Only protein-protein interactions imex curation lt_sv40 Large T antigen protein protein sv40 sv40 SV40 MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDKGGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLFCSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLICKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETKCDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQAVDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLPKMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLNFELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKRTQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALLLMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDDDEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFTCFKKPPTPPPEPET 3E37D4BAFC5D59BD ado2_arath Adagio protein 2 LOV kelch protein 2 Flavin-binding kelch repeat F-box protein 1-like protein 1 F-box only protein 2c ADO2 FKL1 LKP2 At2g18915 F19F24.11 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MQNQMEWDSDSDLSGGDEVAEDGWFGGDNGAIPFPVGSLPGTAPCGFVVSDALEPDNPIIYVNTVFEIVTGYRAEEVIGRNCRFLQCRGPFTKRRHPMVDSTIVAKMRQCLENGIEFQGELLNFRKDGSPLMNKLRLVPIREEDEITHFIGVLLFTDAKIDLGPSPDLSAKEIPRISRSFTSALPIGERNVSRGLCGIFELSDEVIAIKILSQLTPGDIASVGCVCRRLNELTKNDDVWRMVCQNTWGTEATRVLESVPGAKRIGWVRLAREFTTHEATAWRKFSVGGTVEPSRCNFSACAVGNRIVIFGGEGVNMQPMNDTFVLDLGSSSPEWKSVLVSSPPPGRWGHTLSCVNGSRLVVFGGYGSHGLLNDVFLLDLDADPPSWREVSGLAPPIPRSWHSSCTLDGTKLIVSGGCADSGALLSDTFLLDLSMDIPAWREIPVPWTPPSRLGHTLTVYGDRKILMFGGLAKNGTLRFRSNDVYTMDLSEDEPSWRPVIGYGSSLPGGMAAPPPRLDHVAISLPGGRILIFGGSVAGLDSASQLYLLDPNEEKPAWRILNVQGGPPRFAWGHTTCVVGGTRLVVLGGQTGEEWMLNEAHELLLATSTTAST DIP protein Q8W420 original sequence version: 85 FDB9F994CBF0D017 ask20_arath SKP1-like protein 20 ASK20 At2g45950 F4I18.7 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSEGDLAVMKPETMKSYIWLQTADGSIQQVEQEVAMFCPMICQEVIQKGVGSSKNHAISLPQRVNPAMFSLILDYCRFHQLPGRSNKERKTYDERFIRMDTKRLCELTSAADSLQLKPLVDLTSRALARIIEGKNPEEIREIFHLPDDLTEEEKLEPLKNSMDDPRIRLLNRLYAKKRKELKERERLKNVEVEEHVDERSVDDLLSFINGRDHKAVKMSKGKKKKKKKKDQKIVSSNNIHDKESHDLRSKQQCVEEIGSSMREVPNLLSAEDDISTPNAGSEDEDIDDEIDPAMRELLDREVEDFAQRLNSNWVRSLGKERRPVHFSINGNGTTRRHTGMVSIWRPCMFTLF 4C4AA4AE60386258 ado3_arath Adagio protein 3 ADO3 Flavin-binding kelch repeat F-box protein 1 F-box only protein 2a At1g68050 FKF1 T23K23.10 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAREHAIGEATGKRKKRGRVEEAEEYCNDGIEEQVEDEKLPLEVGMFYYPMTPPSFIVSDALEPDFPLIYVNRVFEVFTGYRADEVLGRNCRFLQYRDPRAQRRHPLVDPVVVSEIRRCLEEGIEFQGELLNFRKDGTPLVNRLRLAPIRDDDGTITHVIGIQVFSETTIDLDRVSYPVFKHKQQLDQTSECLFPSGSPRFKEHHEDFCGILQLSDEVLAHNILSRLTPRDVASIGSACRRLRQLTKNESVRKMVCQNAWGKEITGTLEIMTKKLRWGRLARELTTLEAVCWRKFTVGGIVQPSRCNFSACAVGNRLVLFGGEGVNMQPLDDTFVLNLDAECPEWQRVRVTSSPPGRWGHTLSCLNGSWLVVFGGCGRQGLLNDVFVLDLDAKHPTWKEVAGGTPPLPRSWHSSCTIEGSKLVVSGGCTDAGVLLSDTFLLDLTTDKPTWKEIPTSWAPPSRLGHSLSVFGRTKILMFGGLANSGHLKLRSGEAYTIDLEDEEPRWRELECSAFPGVVVPPPRLDHVAVSMPCGRVIIFGGSIAGLHSPSQLFLIDPAEEKPSWRILNVPGKPPKLAWGHSTCVVGGTRVLVLGGHTGEEWILNELHELCLASRQDSDL DIP protein Q9C9W9 original sequence version: 93 AC90C0641149A3D8 srk2a_arath Serine/threonine-protein kinase SRK2A SRK2A At1g10940 T19D16.14 ASK1 OSKL7 SNRK2.4 Arabidopsis protein SK1 OST1-kinase-like 7 SNF1-related kinase 2.4 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDKYELVKDIGAGNFGVARLMKVKNSKELVAMKYIERGPKIDENVAREIINHRSLRHPNIIRFKEVVLTPTHLAIAMEYAAGGELFERICSAGRFSEDEARYFFQQLISGVSYCHAMQICHRDLKLENTLLDGSPAPRLKICDFGYSKSSLLHSRPKSTVGTPAYIAPEVLSRREYDGKMADVWSCGVTLYVMLVGAYPFEDQEDPKNFRKTIQKIMAVQYKIPDYVHISQDCKNLLSRIFVANSLKRITIAEIKKHSWFLKNLPRELTETAQAAYFKKENPTFSLQTVEEIMKIVADAKTPPPVSRSIGGFGWGGNGDADGKEEDAEDVEEEEEEVEEEEDDEDEYDKTVKEVHASGEVRIS DIP protein P43291 original sequence version: 122 89C31C87AB6C6E78 ado1_arath Adagio protein 1 ADO1 FKL2 Protein ZEITLUPE Clock-associated PAS protein ZTL LOV kelch protein 1 Flavin-binding kelch repeat F-box protein 1-like protein 2 F-box only protein 2b LKP1 ZTL MSF19.2 At5g57360 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEWDSGSDLSADDASSLADDEEGGLFPGGGPIPYPVGNLLHTAPCGFVVTDAVEPDQPIIYVNTVFEMVTGYRAEEVLGGNCRFLQCRGPFAKRRHPLVDSMVVSEIRKCIDEGIEFQGELLNFRKDGSPLMNRLRLTPIYGDDDTITHIIGIQFFIETDIDLGPVLGSSTKEKSIDGIYSALAAGERNVSRGMCGLFQLSDEVVSMKILSRLTPRDVASVSSVCRRLYVLTKNEDLWRRVCQNAWGSETTRVLETVPGAKRLGWGRLARELTTLEAAAWRKLSVGGSVEPSRCNFSACAVGNRVVLFGGEGVNMQPMNDTFVLDLNSDYPEWQHVKVSSPPPGRWGHTLTCVNGSNLVVFGGCGQQGLLNDVFVLNLDAKPPTWREISGLAPPLPRSWHSSCTLDGTKLIVSGGCADSGVLLSDTFLLDLSIEKPVWREIPAAWTPPSRLGHTLSVYGGRKILMFGGLAKSGPLKFRSSDVFTMDLSEEEPCWRCVTGSGMPGAGNPGGVAPPPRLDHVAVNLPGGRILIFGGSVAGLHSASQLYLLDPTEDKPTWRILNIPGRPPRFAWGHGTCVVGGTRAIVLGGQTGEEWMLSELHELSLASYLT DIP protein Q94BT6 original sequence version: 95 B25192FCBE019093 ask11_arath SKP1-like protein 11 ASK11 At4g34210 F10M10.2 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSSKMIVLMSSDGQSFEVEEAVAIQSQTIAHMVEDDCVADGIPLANVESKILVKVIEYCKKHHVDEANPISEEDLNNWDEKFMDLEQSTIFELILAANYLNIKSLLDLTCQTVADMIKGKTPEEIRSTFNIENDFTPEEEEAVRKENQWAFE DIP protein O49484 original sequence version: 96 95D23CC17C1523B0 ask14_arath SKP1-like protein 14 At2g03170 ASK14 T18E12.16 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSSNKIVLSSSDGESFEVEEAVARKLKIVEHMIEDDCVVTEVPLQNVTGKILSIVVEYCKKHVVDEESDEFKTWDEEFMKKFDQPTVFQLLLAANYLNIKGLLDLSAQTVADRIKDKTPEEIREIFNIENDFTPEEEAAVRKENAWAFE DIP protein O81057 original sequence version: 91 276BB2CB0AF5DE01 p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD DIP protein P04637 original sequence version: 121 DIP protein P04637 original sequence version: 115 DIP protein P04637-1 original sequence version: 224 DIP protein P04637 original sequence version: 117 DIP protein P04637-1 original sequence version: 226 DIP protein P04637 original sequence version: 226 DIP protein P04637 original sequence version: 212 DIP protein P04637 original sequence version: 220 DIP protein P04637-1 original sequence version: 220 DIP protein P04637-1 original sequence version: 145 DIP protein P04637 original sequence version: 146 DIP protein P04637 original sequence version: 145 DIP protein P04637 original sequence version: 166 DIP protein P04637-1 original sequence version: 166 DIP protein P04637 original sequence version: 169 DIP protein P04637 original sequence version: 177 DIP protein P04637 original sequence version: 175 DIP protein P04637-1 original sequence version: 175 DIP protein P04637-1 original sequence version: 172 DIP protein P04637-1 original sequence version: 169 DIP protein P04637 original sequence version: 170 DIP protein P04637 original sequence version: 172 DIP protein P04637-1 original sequence version: 194 DIP protein P04637 original sequence version: 196 DIP protein P04637 original sequence version: 185 DIP protein P04637-1 original sequence version: 191 DIP protein P04637 original sequence version: 191 DIP protein P04637 original sequence version: 180 DIP protein P04637-1 original sequence version: 197 DIP protein P04637 original sequence version: 183 DIP protein P04637-1 original sequence version: 183 DIP protein P04637-1 original sequence version: 185 DIP protein P04637 original sequence version: 199 DIP protein P04637 original sequence version: 201 DIP protein P04637 original sequence version: 189 DIP protein P04637 original sequence version: 235 DIP protein P04637 original sequence version: 230 DIP protein P04637 original sequence version: 234 DIP protein P04637-1 original sequence version: 234 DIP protein P04637-1 original sequence version: 235 DIP protein P04637 original sequence version: 239 DIP protein P04637-1 original sequence version: 212 DIP protein P04637-1 original sequence version: 213 DIP protein P04637 original sequence version: 213 DIP protein P04637 original sequence version: 206 DIP protein P04637-1 original sequence version: 188 DIP protein P04637-1 original sequence version: 228 DIP protein P04637 original sequence version: 208 DIP protein P04637 original sequence version: 240 DIP protein P04637-1 original sequence version: 240 DIP protein P04637 original sequence version: 204 DIP protein P04637-1 original sequence version: 204 DIP protein P04637-1 original sequence version: 209 DIP protein P04637-1 original sequence version: 203 DIP protein P04637 original sequence version: 221 DIP protein P04637-1 original sequence version: 152 DIP protein P04637 original sequence version: 217 DIP protein P04637-1 original sequence version: 140 DIP protein P04637 original sequence version: 215 DIP protein P04637 original sequence version: 214 DIP protein P04637-1 original sequence version: 182 DIP protein P04637 original sequence version: 182 DIP protein P04637 original sequence version: 188 DIP protein P04637 original sequence version: 192 DIP protein P04637-1 original sequence version: 192 DIP protein P04637 original sequence version: 218 DIP protein P04637-1 original sequence version: 218 AD5C149FD8106131 ask3_arath SKP1-like protein 3 ASK3 At2g25700 F3N11.15 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAETKKMIILKSSDGESFEVEEAVAVESQTIKHMIEDDCVDNGIPLPNVTGAILAKVIEYCKKHVEAAAEAGGDKDFYGSTENHELKTWDNDFVKVDHPTLFDLLRAANYLNISGLLDLTCKAVADQMRGKTPAQMREHFNIKNDYTPEEEAEVRNENRWAFE 8F7C7796CE5CEB4F ask4_arath SKP1-like protein 4 T20H2.8 At1g20140 ASK4 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAETKKMIILKSSDGESFEIEEAVAVKSQTIKHMIEDDCADNGIPLPNVTGAILAKVIEYCKKHVEAAAEAGGDKDFYGSAENDELKNWDSEFVKVDQPTLFDLILAANYLNIGGLLDLTCKAVADQMRGKTPEQMRAHFNIKNDYTPEEEAEVRNENKWAFE DIP protein Q9LNT9 original sequence version: 89 9B0AD6B569A5F932 aprr5_arath Two-component response regulator-like APRR5 APRR5 T31K7.5 At5g24470 Pseudo-response regulator 5 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTSSEEVVEVTVVKAPEAGGGKLSRRKIRKKDAGVDGLVKWERFLPKIALRVLLVEADDSTRQIIAALLRKCSYRVAAVPDGLKAWEMLKGKPESVDLILTEVDLPSISGYALLTLIMEHDICKNIPVIMMSTQDSVNTVYKCMLKGAADYLVKPLRRNELRNLWQHVWRRQTSLAPDSFPWNESVGQQKAEGASANNSNGKRDDHVVSGNGGDAQSSCTRPEMEGESADVEVSARDAVQMECAKSQFNETRLLANELQSKQAEAIDFMGASFRRTGRRNREESVAQYESRIELDLSLRRPNASENQSSGDRPSLHPSSASAFTRYVHRPLQTQCSASPVVTDQRKNVAASQDDNIVLMNQYNTSEPPPNAPRRNDTSFYTGADSPGPPFSNQLNSWPGQSSYPTPTPINNIQFRDPNTAYTSAMAPASLSPSPSSVSPHEYSSMFHPFNSKPEGLQDRDCSMDVDERRYVSSATEHSAIGNHIDQLIEKKNEDGYSLSVGKIQQSLQREAALTKFRMKRKDRCYEKKVRYESRKKLAEQRPRIKGQFVRQVQSTQAP 9813089FA9DC8CE9 ask13_arath SKP1-like protein 13 ASK13 At3g60010 F24G16.280 T2O9.1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSKMVMLLSSDGESFQVEEAVAVQSQTIAHMIEDDCVANGVPIANVTGVILSKVIEYCKKHVVSDSPTEESKDELKKWDAEFMKALEQSSTLFDVMLAANYLNIKDLLDLGCQTVADMITGKKPDEIRALLGIENDFTPEEEEEIRKENQWAFE DIP protein Q9M1X5 original sequence version: 75 FF771FCF3B9844A5 aprr1_arath Two-component response regulator-like APRR1 APRR1 AIP1 TOC1 At5g61380 MFB13.16 Pseudo-response regulator 1 Timing of CAB expression 1 ABI3-interacting protein 1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDLNGECKGGDGFIDRSRVRILLCDNDSTSLGEVFTLLSECSYQVTAVKSARQVIDALNAEGPDIDIILAEIDLPMAKGMKMLRYITRDKDLRRIPVIMMSRQDEVPVVVKCLKLGAADYLVKPLRTNELLNLWTHMWRRRRMLGLAEKNMLSYDFDLVGSDQSDPNTNSTNLFSDDTDDRSLRSTNPQRGNLSHQENEWSVATAPVHARDGGLGADGTATSSLAVTAIEPPLDHLAGSHHEPMKRNSNPAQFSSAPKKSRLKIGESSAFFTYVKSTVLRTNGQDPPLVDGNGSLHLHRGLAEKFQVVASEGINNTKQARRATPKSTVLRTNGQDPPLVNGNGSHHLHRGAAEKFQVVASEGINNTKQAHRSRGTEQYHSQGETLQNGASYPHSLERSRTLPTSMESHGRNYQEGNMNIPQVAMNRSKDSSQVDGSGFSAPNAYPYYMHGVMNQVMMQSAAMMPQYGHQIPHCQPNHPNGMTGYPYYHHPMNTSLQHSQMSLQNGQMSMVHHSWSPAGNPPSNEVRVNKLDRREEALLKFRRKRNQRCFDKKIRYVNRKRLAERRPRVKGQFVRKMNGVNVDLNGQPDSADYDDEEEEEEEEEEENRDSSPQDDALGT DIP protein Q9LKL2 original sequence version: 103 EDBE0AA0B7FAB61E skp1b_arath SKP1-like protein 1B SKP1-like 2 UFO-binding protein 2 ASK2 UIP2 At5g42190 MJC20.30 SKP1B protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSTVRKITLKSSDGENFEIDEAVALESQTIKHMIEDDCTDNGIPLPNVTSKILSKVIEYCKRHVEAAEKSETTADAAAATTTTTVASGSSDEDLKTWDSEFIKVDQGTLFDLILAANYLNIKGLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE DIP protein Q9FHW7 original sequence version: 101 DIP protein Q9FHW7 original sequence version: 104 DIP protein Q9FHW7 original sequence version: 86 9695CC9F57FC935F lkp1-ask1 1 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region F Box binding region binding-associated region binding region range range range range physical association physical association The amino acid coordinates of the region denoted as the F box is not given by the authors. Therefore the region described as containing the F box from the UniProt entry is provided. Since the exact coordinates are not known, the coordinates are given as 'fuzzy' coordinates. 3B The F-box regions of LKP1, LKP2, and FKF1 are sufficient to bind to ASK proteins (Fig. 3B-D). lkp1-ask2 1 7 unspecified role unspecified role bait bait region F Box binding region binding-associated region binding region range range range range 16 unspecified role unspecified role prey prey physical association physical association The amino acid coordinates of the region denoted as the F box is not given by the authors. Therefore the region described as containing the F box from the UniProt entry is provided. Since the exact coordinates are not known, the coordinates are given as 'fuzzy' coordinates. 3B The F-box regions of LKP1, LKP2, and FKF1 are sufficient to bind to ASK proteins (Fig. 3B-D). lkp1-ask4 1 12 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region F Box binding region binding-associated region binding region range range range range physical association physical association The amino acid coordinates of the region denoted as the F box is not given by the authors. Therefore the region described as containing the F box from the UniProt entry is provided. Since the exact coordinates are not known, the coordinates are given as 'fuzzy' coordinates. 3B The F-box regions of LKP1, LKP2, and FKF1 are sufficient to bind to ASK proteins (Fig. 3B-D). lkp1-ask11 1 7 unspecified role unspecified role bait bait region F Box binding region binding-associated region binding region range range range range 8 unspecified role unspecified role prey prey physical association physical association The amino acid coordinates of the region denoted as the F box is not given by the authors. Therefore the region described as containing the F box from the UniProt entry is provided. Since the exact coordinates are not known, the coordinates are given as 'fuzzy' coordinates. 3B The F-box regions of LKP1, LKP2, and FKF1 are sufficient to bind to ASK proteins (Fig. 3B-D). lkp1-ask14 1 7 unspecified role unspecified role bait bait region F Box binding region binding-associated region binding region range range range range 9 unspecified role unspecified role prey prey physical association physical association The amino acid coordinates of the region denoted as the F box is not given by the authors. Therefore the region described as containing the F box from the UniProt entry is provided. Since the exact coordinates are not known, the coordinates are given as 'fuzzy' coordinates. 3B The F-box regions of LKP1, LKP2, and FKF1 are sufficient to bind to ASK proteins (Fig. 3B-D). p53-sv40t-4 1 10 unspecified role unspecified role prey prey 2 unspecified role unspecified role bait bait physical association physical association 3B positive control yeat two hybrid interaction lkp2-ask1 1 3 unspecified role unspecified role bait bait region binding region binding-associated region binding region range range range range 6 unspecified role unspecified role prey prey physical association physical association The amino acid coordinates of the region denoted as the F box is not given by the authors. Therefore the region described as containing the F box from the UniProt entry is provided. Since the exact coordinates are not known, the coordinates are given as 'fuzzy' coordinates. 3C The F-box regions of LKP1, LKP2, and FKF1 are sufficient to bind to ASK proteins (Fig. 3B-D). lkp2-ask2 1 16 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait region binding region binding-associated region binding region range range range range physical association physical association The amino acid coordinates of the region denoted as the F box is not given by the authors. Therefore the region described as containing the F box from the UniProt entry is provided. Since the exact coordinates are not known, the coordinates are given as 'fuzzy' coordinates. 3C The F-box regions of LKP1, LKP2, and FKF1 are sufficient to bind to ASK proteins (Fig. 3B-D). lkp2-ask4 1 12 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait region binding region binding-associated region binding region range range range range physical association physical association The amino acid coordinates of the region denoted as the F box is not given by the authors. Therefore the region described as containing the F box from the UniProt entry is provided. Since the exact coordinates are not known, the coordinates are given as 'fuzzy' coordinates. 3C The F-box regions of LKP1, LKP2, and FKF1 are sufficient to bind to ASK proteins (Fig. 3B-D). lkp2-ask11-1 1 3 unspecified role unspecified role bait bait region binding region binding-associated region binding region range range range range 8 unspecified role unspecified role prey prey physical association physical association The amino acid coordinates of the region denoted as the F box is not given by the authors. Therefore the region described as containing the F box from the UniProt entry is provided. Since the exact coordinates are not known, the coordinates are given as 'fuzzy' coordinates. 3C The F-box regions of LKP1, LKP2, and FKF1 are sufficient to bind to ASK proteins (Fig. 3B-D). lkp2-ask14 1 3 unspecified role unspecified role bait bait region binding region binding-associated region binding region range range range range 9 unspecified role unspecified role prey prey physical association physical association The amino acid coordinates of the region denoted as the F box is not given by the authors. Therefore the region described as containing the F box from the UniProt entry is provided. Since the exact coordinates are not known, the coordinates are given as 'fuzzy' coordinates. 3C The F-box regions of LKP1, LKP2, and FKF1 are sufficient to bind to ASK proteins (Fig. 3B-D). lkp2-ask3 1 3 unspecified role unspecified role bait bait region binding region binding-associated region binding region range range range range 11 unspecified role unspecified role prey prey physical association physical association The amino acid coordinates of the region denoted as the F box is not given by the authors. Therefore the region described as containing the F box from the UniProt entry is provided. Since the exact coordinates are not known, the coordinates are given as 'fuzzy' coordinates. 3C The F-box regions of LKP1, LKP2, and FKF1 are sufficient to bind to ASK proteins (Fig. 3B-D). lkp2-ask5 1 14 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait region binding region binding-associated region binding region range range range range physical association physical association The amino acid coordinates of the region denoted as the F box is not given by the authors. Therefore the region described as containing the F box from the UniProt entry is provided. Since the exact coordinates are not known, the coordinates are given as 'fuzzy' coordinates. 3C The F-box regions of LKP1, LKP2, and FKF1 are sufficient to bind to ASK proteins (Fig. 3B-D). lkp2-ask20 1 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait region binding region binding-associated region binding region range range range range physical association physical association The amino acid coordinates of the region denoted as the F box is not given by the authors. Therefore the region described as containing the F box from the UniProt entry is provided. Since the exact coordinates are not known, the coordinates are given as 'fuzzy' coordinates. 3C The F-box regions of LKP1, LKP2, and FKF1 are sufficient to bind to ASK proteins (Fig. 3B-D). fkf1-ask1 1 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait region binding region binding-associated region binding region range range range range physical association physical association The amino acid coordinates of the region denoted as the F box is not given by the authors. Therefore the region described as containing the F box from the UniProt entry is provided. Since the exact coordinates are not known, the coordinates are given as 'fuzzy' coordinates. 3D The F-box regions of LKP1, LKP2, and FKF1 are sufficient to bind to ASK proteins (Fig. 3B-D). fkf1-ask2-1 1 5 unspecified role unspecified role bait bait region binding region binding-associated region binding region range range range range 16 unspecified role unspecified role prey prey physical association physical association The amino acid coordinates of the region denoted as the F box is not given by the authors. Therefore the region described as containing the F box from the UniProt entry is provided. Since the exact coordinates are not known, the coordinates are given as 'fuzzy' coordinates. 3D The F-box regions of LKP1, LKP2, and FKF1 are sufficient to bind to ASK proteins (Fig. 3B-D). fkf1-ask11 1 5 unspecified role unspecified role bait bait region binding region binding-associated region binding region range range range range 8 unspecified role unspecified role prey prey physical association physical association The amino acid coordinates of the region denoted as the F box is not given by the authors. Therefore the region described as containing the F box from the UniProt entry is provided. Since the exact coordinates are not known, the coordinates are given as 'fuzzy' coordinates. 3D The F-box regions of LKP1, LKP2, and FKF1 are sufficient to bind to ASK proteins (Fig. 3B-D). fkf1-ask14 1 5 unspecified role unspecified role bait bait region binding region binding-associated region binding region range range range range 9 unspecified role unspecified role prey prey physical association physical association The amino acid coordinates of the region denoted as the F box is not given by the authors. Therefore the region described as containing the F box from the UniProt entry is provided. Since the exact coordinates are not known, the coordinates are given as 'fuzzy' coordinates. 3D The F-box regions of LKP1, LKP2, and FKF1 are sufficient to bind to ASK proteins (Fig. 3B-D). lkp2-lkp2-1 1 3 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey physical association physical association 4 Interactions were observed between LKP2 and LKP1, LKP2 and LKP2, and LKP2 and FKF1, but none were detected between LKP1 and LKP1, or LKP1 and FKF1 by yeast growth tests on restricted medium lkp2-lkp2 1 7 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association 4 Interactions were observed between LKP2 and LKP1, LKP2 and LKP2, and LKP2 and FKF1, but none were detected between LKP1 and LKP1, or LKP1 and FKF1 by yeast growth tests on restricted medium lkp2-fkf1 1 3 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association 4 Interactions were observed between LKP2 and LKP1, LKP2 and LKP2, and LKP2 and FKF1, but none were detected between LKP1 and LKP1, or LKP1 and FKF1 by yeast growth tests on restricted medium lkp1-toc1 1 7 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey physical association physical association 5 a strong interaction of LKP2 and TOC1 was detected, and a weak interaction of LKP1 and TOC1 was detected A weak interaction of LKP1 and TOC1 was detected lkp2-toc1-1 1 3 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey physical association physical association 5 a strong interaction of LKP2 and TOC1 was detected, and a weak interaction of LKP1 and TOC1 was detected A strong interaction of LKP2 and TOC1 was detected, lkp1-aprr5 1 13 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association 7 a strong interaction was detected between LKP2 and APRR5, and a weak interaction was detected between LKP1 and APRR5. A weak interaction of LKP1 and APRR5 was detected lkp2-aprr5 1 3 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey physical association physical association 7 a strong interaction was detected between LKP2 and APRR5, and a weak interaction was detected between LKP1 and APRR5. A strong interaction of LKP2 and APRR5 was detected,