IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ TRB3: a tribbles homolog that inhibits Akt/PKB activation by insulin in liver. TRB3: a tribbles homolog that inhibits Akt/PKB activation by insulin in liver. Science (0036-8075) 2003 Du K., Herzig S., Kulkarni RN., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis Used preadipocyte F422A cDNA library human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). anti bait coip anti bait coimmunoprecipitation anti bait coip polyclonal western polyclonal antibody western blot polyclonal western Du K., Herzig S., Kulkarni RN., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis Used preadipocyte F422A cDNA library Science (0036-8075) 2003 TRB3: a tribbles homolog that inhibits Akt/PKB activation by insulin in liver. TRB3: a tribbles homolog that inhibits Akt/PKB activation by insulin in liver. Science (0036-8075) 2003 Du K., Herzig S., Kulkarni RN., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis Used preadipocyte F422A cDNA library yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Du K., Herzig S., Kulkarni RN., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis Used preadipocyte F422A cDNA library Science (0036-8075) 2003 TRB3: a tribbles homolog that inhibits Akt/PKB activation by insulin in liver. TRB3: a tribbles homolog that inhibits Akt/PKB activation by insulin in liver. Science (0036-8075) 2003 Du K., Herzig S., Kulkarni RN., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis Used preadipocyte F422A cDNA library human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Du K., Herzig S., Kulkarni RN., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis Used preadipocyte F422A cDNA library Science (0036-8075) 2003 TRB3: a tribbles homolog that inhibits Akt/PKB activation by insulin in liver. TRB3: a tribbles homolog that inhibits Akt/PKB activation by insulin in liver. Science (0036-8075) 2003 Du K., Herzig S., Kulkarni RN., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis Used preadipocyte F422A cDNA library human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Du K., Herzig S., Kulkarni RN., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis Used preadipocyte F422A cDNA library Science (0036-8075) 2003 TRB3: a tribbles homolog that inhibits Akt/PKB activation by insulin in liver. TRB3: a tribbles homolog that inhibits Akt/PKB activation by insulin in liver. Science (0036-8075) 2003 Du K., Herzig S., Kulkarni RN., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis Used preadipocyte F422A cDNA library in vitro In vitro pull down pull down predetermined predetermined participant predetermined Du K., Herzig S., Kulkarni RN., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis Used preadipocyte F422A cDNA library Science (0036-8075) 2003 trib3_mouse Tribbles homolog 3 Neuronal cell death-inducible putative kinase Nipk Trb3 Trib3 protein protein mouse Mus musculus Mouse MRATPLAASADVSCRKKPLEFDDNIDAKCPVLKRVRDEPEPGPLPSLLPPSPPPASDLSPAVAPATRLGPYILLEREQGSCSYRALHCPTGTEYTCKVYPASEAQAVLAPYARLPTHQHVARPTEVLLGSRLLYIFFTKTHGDLHSLVRSRRGIPESEAAGLFRQMASAVAHCHKHGLVLRDLKLRRFVFSNCERTKLVLENLEDACVMTGSDDSLWDKHACPAYVGPEILSSRPSYSGKAADVWSLGVALFTMLAGRYPFHDSEPVLLFGKIRRGTFALPEGLSAPARCLIRCLLRKEPSERLVALGILLHPWLREDHGRVSPPQSDRREMDQVVPDGPQLEEAEEGEVGLYG DIP protein Q8K4K2 original sequence version: 88 2CB283FC119F859F akt1_mouse RAC-alpha serine/threonine-protein kinase Akt1 Akt Rac RAC-PK-alpha AKT1 kinase Proto-oncogene c-Akt Protein kinase B Thymoma viral proto-oncogene Protein kinase B alpha protein protein mouse Mus musculus Mouse MNDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDVDQRESPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWATAIQTVADGLKRQEEETMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDRLCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLMLDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPTQRLGGGSEDAKEIMQHRFFANIVWQDVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSMECVDSERRPHFPQFSYSASGTA DIP protein P31750 original sequence version: 125 DIP protein P31750 original sequence version: 168 DIP protein P31750 original sequence version: 88 98DF28E5EFE03730 akt1-trib3-1 2 7 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey physical association physical association akt1-trib3-2 3 7 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey physical association physical association akt1-trib3-3 5 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey 35s radiolabel radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association akt1-trib3-4 1 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association akt1-trib3-5 4 7 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association