IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ EBV-encoded EBNA-5 associates with P14ARF in extranucleolar inclusions and prolongs the survival of P14ARF-expressing cells. EBV-encoded EBNA-5 associates with P14ARF in extranucleolar inclusions and prolongs the survival of P14ARF-expressing cells. Int. J. Cancer (0020-7136) 2003 Kashuba E., Mattsson K., Pokrovskaja K., Kiss C., Protopopova M., Ehlin-Henriksson B., Klein G., Szekely L. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer elena.kashuba@mtc.ki.se 22-Jan-2008: Contacted by JYOTI. davet yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kashuba E., Mattsson K., Pokrovskaja K., Kiss C., Protopopova M., Ehlin-Henriksson B., Klein G., Szekely L. Apoptosis - Interactions involving proteins with a function related to apoptosis elena.kashuba@mtc.ki.se Accepted 2007-Not a month!-18 by DAVET. Int. J. Cancer (0020-7136) 2003 davet EBV-encoded EBNA-5 associates with P14ARF in extranucleolar inclusions and prolongs the survival of P14ARF-expressing cells. EBV-encoded EBNA-5 associates with P14ARF in extranucleolar inclusions and prolongs the survival of P14ARF-expressing cells. Int. J. Cancer (0020-7136) 2003 Kashuba E., Mattsson K., Pokrovskaja K., Kiss C., Protopopova M., Ehlin-Henriksson B., Klein G., Szekely L. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer elena.kashuba@mtc.ki.se 22-Jan-2008: Contacted by JYOTI. davet in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Kashuba E., Mattsson K., Pokrovskaja K., Kiss C., Protopopova M., Ehlin-Henriksson B., Klein G., Szekely L. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of protein p14ARF taken from PMID 10772813 elena.kashuba@mtc.ki.se Accepted 2007-Not a month!-18 by DAVET. 22-Jan-2008: Contacted by JYOTI. Int. J. Cancer (0020-7136) 2003 davet EBV-encoded EBNA-5 associates with P14ARF in extranucleolar inclusions and prolongs the survival of P14ARF-expressing cells. EBV-encoded EBNA-5 associates with P14ARF in extranucleolar inclusions and prolongs the survival of P14ARF-expressing cells. Int. J. Cancer (0020-7136) 2003 Kashuba E., Mattsson K., Pokrovskaja K., Kiss C., Protopopova M., Ehlin-Henriksson B., Klein G., Szekely L. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer elena.kashuba@mtc.ki.se 22-Jan-2008: Contacted by JYOTI. davet human-m1 Homo sapiens fibroblast cell line m1 M1 human fibrobast murine myeloid cell line fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Kashuba E., Mattsson K., Pokrovskaja K., Kiss C., Protopopova M., Ehlin-Henriksson B., Klein G., Szekely L. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of p14ARF taken from PMID 10772813 elena.kashuba@mtc.ki.se Accepted 2007-Not a month!-18 by DAVET. 22-Jan-2008: Contacted by JYOTI. Int. J. Cancer (0020-7136) 2003 davet EBV-encoded EBNA-5 associates with P14ARF in extranucleolar inclusions and prolongs the survival of P14ARF-expressing cells. EBV-encoded EBNA-5 associates with P14ARF in extranucleolar inclusions and prolongs the survival of P14ARF-expressing cells. Int. J. Cancer (0020-7136) 2003 Kashuba E., Mattsson K., Pokrovskaja K., Kiss C., Protopopova M., Ehlin-Henriksson B., Klein G., Szekely L. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer elena.kashuba@mtc.ki.se 22-Jan-2008: Contacted by JYOTI. davet human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Kashuba E., Mattsson K., Pokrovskaja K., Kiss C., Protopopova M., Ehlin-Henriksson B., Klein G., Szekely L. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of p14ARF taken from PMID 10772813 elena.kashuba@mtc.ki.se Accepted 2007-Not a month!-18 by DAVET. 22-Jan-2008: Contacted by JYOTI. Int. J. Cancer (0020-7136) 2003 davet pml_human Protein PML PML MYL RNF71 TRIM19 Tripartite motif-containing protein 19 RING finger protein 71 Promyelocytic leukemia protein PP8675 E3 SUMO-protein ligase PML RING-type E3 SUMO transferase PML protein protein human Homo sapiens Human MEPAPARSPRPQQDPARPQEPTMPPPETPSEGRQPSPSPSPTERAPASEEEFQFLRCQQCQAEAKCPKLLPCLHTLCSGCLEASGMQCPICQAPWPLGADTPALDNVFFESLQRRLSVYRQIVDAQAVCTRCKESADFWCFECEQLLCAKCFEAHQWFLKHEARPLAELRNQSVREFLDGTRKTNNIFCSNPNHRTPTLTSIYCRGCSKPLCCSCALLDSSHSELKCDISAEIQQRQEELDAMTQALQEQDSAFGAVHAQMHAAVGQLGRARAETEELIRERVRQVVAHVRAQERELLEAVDARYQRDYEEMASRLGRLDAVLQRIRTGSALVQRMKCYASDQEVLDMHGFLRQALCRLRQEEPQSLQAAVRTDGFDEFKVRLQDLSSCITQGKDAAVSKKASPEAASTPRDPIDVDLPEEAERVKAQVQALGLAEAQPMAVVQSVPGAHPVPVYAFSIKGPSYGEDVSNTTTAQKRKCSQTQCPRKVIKMESEEGKEARLARSSPEQPRPSTSKAVSPPHLDGPPSPRSPVIGSEVFLPNSNHVASGAGEAEERVVVISSSEDSDAENSSSRELDDSSSESSDLQLEGPSTLRVLDENLADPQAEDRPLVFFDLKIDNETQKISQLAAVNRESKFRVVIQPEAFFSIYSKAVSLEVGLQHFLSFLSSMRRPILACYKLWGPGLPNFFRALEDINRLWEFQEAISGFLAALPLIRERVPGASSFKLKNLAQTYLARNMSERSAMAAVLAMRDLCRLLEVSPGPQLAQHVYPFSSLQCFASLQPLVQAAVLPRAEARLLALHNVSFMELLSAHRRDRQGGLKKYSRYLSLQTTTLPPAQPAFNLQALGTYFEGLLEGPALARAEGVSTPLAGRGLAERASQQS DIP protein P29590 original sequence version: 188 DIP protein P29590 original sequence version: 195 DIP protein P29590-1 original sequence version: 187 D50968A977E34287 ebna5_ebvb9 Epstein-Barr nuclear antigen leader protein EBNA-LP EBNA5 Epstein-Barr nuclear antigen 5 protein protein ebvb9 Epstein-Barr virus (strain B95-8) (HHV-4) Human herpesvirus 4 HHV-4 MGDRSEGPGPTRPGPPGIGPEGPLGQLLRRHRSPSPTRGGQEPRRVRRRVLVQQEEEVVSGSPSGPRGDRSEGPGPTRPGPPGIGPEGPLGQLLRRHRSPSPTRGGQEPRRVRRRVLVQQEEEVVSGSPSGPRGDRSEGPGPTRPGPPGIGPEGPLGQLLRRHRSPSPTRGGQEPRRVRRRVLVQQEEEVVSGSPSGPRGDRSEGPGPTRPGPPGIGPEGPLGQLLRRHRSPSPTRGGQEPRRVRRRVLVQQEEEVVSGSPSGPRGDRSEGPGPTRPGPPGIGPEGPLGQLLRRHRSPSPTRGGQEPRRVRRRVLVQQEEEVVSGSPSGPRGDRSEGPGPTRPGPPGIGPEGPLGQLLRRHRSPSPTRGGQEPRRVRRRVLVQQEEEVVSGSPSGPRGDRSEGPGPTRPGPPGIGPEGPLGQLLRRHRSPSPTRGGQEPRRVRRRVLVQQEEEVVSGSPSGPLRPRPRPPARSLREWLLRIRDHFEPPTVTTQRQSVYIEEEEDED DIP protein Q8AZK7 original sequence version: 59 CD2C6C3B737AC1E9 arf_human Tumor suppressor ARF p14ARF CDKN2A CDKN2 MLM Alternative reading frame Cyclin-dependent kinase inhibitor 2A protein protein human Homo sapiens Human MVRRFLVTLRIRRACGPPRVRVFVVHIPRLTGEWAAPGAPAAVALVLMLLRSQRLGQQPLPRRPGHDDGQRPSGGAAAAPRRGAQLRRPRHSHPTRARRCPGGLPGHAGGAAPGRGAAGRARCLGPSARGPG DIP protein Q8N726 original sequence version: 97 DIP protein Q8N726 original sequence version: 113 DIP protein Q8N726 original sequence version: 72 7739A9050C21BC96 p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD DIP protein P04637 original sequence version: 121 DIP protein P04637 original sequence version: 115 DIP protein P04637-1 original sequence version: 224 DIP protein P04637 original sequence version: 117 DIP protein P04637-1 original sequence version: 226 DIP protein P04637 original sequence version: 226 DIP protein P04637 original sequence version: 212 DIP protein P04637 original sequence version: 220 DIP protein P04637-1 original sequence version: 220 DIP protein P04637-1 original sequence version: 145 DIP protein P04637 original sequence version: 146 DIP protein P04637 original sequence version: 145 DIP protein P04637 original sequence version: 166 DIP protein P04637-1 original sequence version: 166 DIP protein P04637 original sequence version: 169 DIP protein P04637 original sequence version: 177 DIP protein P04637 original sequence version: 175 DIP protein P04637-1 original sequence version: 175 DIP protein P04637-1 original sequence version: 172 DIP protein P04637-1 original sequence version: 169 DIP protein P04637 original sequence version: 170 DIP protein P04637 original sequence version: 172 DIP protein P04637-1 original sequence version: 194 DIP protein P04637 original sequence version: 196 DIP protein P04637 original sequence version: 185 DIP protein P04637-1 original sequence version: 191 DIP protein P04637 original sequence version: 191 DIP protein P04637 original sequence version: 180 DIP protein P04637-1 original sequence version: 197 DIP protein P04637 original sequence version: 183 DIP protein P04637-1 original sequence version: 183 DIP protein P04637-1 original sequence version: 185 DIP protein P04637 original sequence version: 199 DIP protein P04637 original sequence version: 201 DIP protein P04637 original sequence version: 189 DIP protein P04637 original sequence version: 235 DIP protein P04637 original sequence version: 230 DIP protein P04637 original sequence version: 234 DIP protein P04637-1 original sequence version: 234 DIP protein P04637-1 original sequence version: 235 DIP protein P04637 original sequence version: 239 DIP protein P04637-1 original sequence version: 212 DIP protein P04637-1 original sequence version: 213 DIP protein P04637 original sequence version: 213 DIP protein P04637 original sequence version: 206 DIP protein P04637-1 original sequence version: 188 DIP protein P04637-1 original sequence version: 228 DIP protein P04637 original sequence version: 208 DIP protein P04637 original sequence version: 240 DIP protein P04637-1 original sequence version: 240 DIP protein P04637 original sequence version: 204 DIP protein P04637-1 original sequence version: 204 DIP protein P04637-1 original sequence version: 209 DIP protein P04637-1 original sequence version: 203 DIP protein P04637 original sequence version: 221 DIP protein P04637-1 original sequence version: 152 DIP protein P04637 original sequence version: 217 DIP protein P04637-1 original sequence version: 140 DIP protein P04637 original sequence version: 215 DIP protein P04637 original sequence version: 214 DIP protein P04637-1 original sequence version: 182 DIP protein P04637 original sequence version: 182 DIP protein P04637 original sequence version: 188 DIP protein P04637 original sequence version: 192 DIP protein P04637-1 original sequence version: 192 DIP protein P04637 original sequence version: 218 DIP protein P04637-1 original sequence version: 218 AD5C149FD8106131 mdm2_human E3 ubiquitin-protein ligase Mdm2 MDM2 p53-binding protein Mdm2 Oncoprotein Mdm2 Double minute 2 protein RING-type E3 ubiquitin transferase Mdm2 protein protein human Homo sapiens Human MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP DIP protein Q00987 original sequence version: 93 DIP protein Q00987-1 original sequence version: 192 DIP protein Q00987 original sequence version: 88 DIP protein Q00987 original sequence version: 180 DIP protein Q00987 original sequence version: 188 DIP protein Q00987 original sequence version: 189 DIP protein Q00987 original sequence version: 115 DIP protein Q00987-1 original sequence version: null DIP protein Q00987 original sequence version: 149 DIP protein Q00987 original sequence version: 146 DIP protein Q00987 original sequence version: 140 DIP protein Q00987 original sequence version: 183 DIP protein Q00987 original sequence version: 145 DIP protein Q00987 original sequence version: 182 DIP protein Q00987 original sequence version: 151 DIP protein Q00987 original sequence version: 156 DIP protein Q00987-1 original sequence version: 160 DIP protein Q00987 original sequence version: 160 DIP protein Q00987 original sequence version: 168 DIP protein Q00987 original sequence version: 186 DIP protein Q00987 original sequence version: 206 F37CE163876BC983 cdkn2a-ebna_lp 1 7 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association 1 Only coexpression of EBNA-5 and p14ARF resulted in significant activation of the reporter lacZ gene. The strength of interaction was 6% of the strong SV40 LT-p53 or 8.3% of SV40LT-Rb interactions as measured by -galactosidase units in 4 independent cotransfection experiments. This interaction was not dependent on the configuration of the constructs. It was also detected when EBNA-5 and p14ARF were swapped in the AD/BD vectors. The EBNA-LP protein referred to by UniprotKB accession number Q8AZK7 is from strain GD1 of EBV. However, the authors do not specify which strain their construct comes from. ebna_lp-cdkn2a 1 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association 1 Only coexpression of EBNA-5 and p14ARF resulted in significant activation of the reporter lacZ gene. The strength of interaction was 6% of the strong SV40 LT-p53 or 8.3% of SV40LT-Rb interactions as measured by -galactosidase units in 4 independent cotransfection experiments. This interaction was not dependent on the configuration of the constructs. It was also detected when EBNA-5 and p14ARF were swapped in the AD/BD vectors. The EBNA-LP protein referred to by UniprotKB accession number Q8AZK7 is from strain GD1 of EBV. However, the authors do not specify which strain their construct comes from. cdkn2a-ebna_lp-1 2 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey human-colon human colon colon physical association physical association only full-length GST-2TK-p14ARF could precipitate EBNA-5 from the lysates. Neither the N- nor the C-terminus deleted constructs, which lack amino acids 1-55 and 65-132, respectively, bound EBNA-5 2 The EBNA-LP protein referred to by Uniprotkb accession number Q8AZK7 is from strain GD1 of EBV. However, the authors do not specify which strain their construct comes from. cdkn2a-ebna_lp-2 4 7 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role neutral component neutral component colocalization colocalization The EBNA-LP protein referred to by Uniprotkb accession number Q8AZK7 is from strain GD1 of EBV. However, the authors do not specify which strain their construct comes from. 4 A Expression of GFPp14ARF induces the relocalization of EBNA-5 from homogeneous nucleoplasmic distribution into nuclear inclusions in MCF7 cells double transfected with pBABE EBNA-5 Q8N726; Q8AZK7 transfection cdkn2a-pml 3 7 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component colocalization colocalization 5 A p14ARF positive nuclear inclusions (hollow arrowheads) but not nucleoli (full arrowheads) accumulate PML bodies Q8N726 transfection cdkn2a-mdm2 3 7 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component colocalization colocalization 5 C where p14ARF was present both in the nucleoli and in the inclusions, EBNA-5, p53 and HDM2 accumulated exclusively in the inclusions.The expansion of nuclear inclusions was paralleled by the complete relocation of EBNA-5 (Fig. 4c), p53 (Fig. 5d) and HDM2 (Fig. 5c) to these sites. Q8N726 transfection cdkn2a-tp53 3 7 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization 5 D where p14ARF was present both in the nucleoli and in the inclusions, EBNA-5, p53 and HDM2 accumulated exclusively in the inclusions.The expansion of nuclear inclusions was paralleled by the complete relocation of EBNA-5 (Fig. 4c), p53 (Fig. 5d) and HDM2 (Fig. 5c) to these sites. Q8N726 transfection cdkn2a-tp53-1 3 7 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component colocalization colocalization 4 C Q8N726 transfection transfection of GFP-p14ARF or pBabe-p14ARF into MCF7 that constitutively expressed EBNA-5 (M1 cells) led to the relocation of EBNA-5 into the p14ARF-positive nuclear inclusions