IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Induction of colonic epithelial cell apoptosis by p47-dependent oxidants. Induction of colonic epithelial cell apoptosis by p47-dependent oxidants. FEBS Lett. (0014-5793) 2003 imex curation Gu Y., Souza RF., Wu RF., Xu YC., Terada LS. Apoptosis - Interactions involving proteins with a function related to apoptosis Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Gu Y., Souza RF., Wu RF., Xu YC., Terada LS. Apoptosis - Interactions involving proteins with a function related to apoptosis interaction detected by yeast mating experiments FEBS Lett. (0014-5793) 2003 IMEx Induction of colonic epithelial cell apoptosis by p47-dependent oxidants. Induction of colonic epithelial cell apoptosis by p47-dependent oxidants. FEBS Lett. (0014-5793) 2003 imex curation Gu Y., Souza RF., Wu RF., Xu YC., Terada LS. Apoptosis - Interactions involving proteins with a function related to apoptosis Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gu Y., Souza RF., Wu RF., Xu YC., Terada LS. Apoptosis - Interactions involving proteins with a function related to apoptosis FEBS Lett. (0014-5793) 2003 IMEx Induction of colonic epithelial cell apoptosis by p47-dependent oxidants. Induction of colonic epithelial cell apoptosis by p47-dependent oxidants. FEBS Lett. (0014-5793) 2003 imex curation Gu Y., Souza RF., Wu RF., Xu YC., Terada LS. Apoptosis - Interactions involving proteins with a function related to apoptosis Only protein-protein interactions imex curation in vitro In vitro pull down pull down predetermined predetermined participant predetermined Gu Y., Souza RF., Wu RF., Xu YC., Terada LS. Apoptosis - Interactions involving proteins with a function related to apoptosis FEBS Lett. (0014-5793) 2003 IMEx Induction of colonic epithelial cell apoptosis by p47-dependent oxidants. Induction of colonic epithelial cell apoptosis by p47-dependent oxidants. FEBS Lett. (0014-5793) 2003 imex curation Gu Y., Souza RF., Wu RF., Xu YC., Terada LS. Apoptosis - Interactions involving proteins with a function related to apoptosis Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Gu Y., Souza RF., Wu RF., Xu YC., Terada LS. Apoptosis - Interactions involving proteins with a function related to apoptosis FEBS Lett. (0014-5793) 2003 IMEx abi1_human Abl interactor 1 ABI1 SSH3BP1 Abelson interactor 1 Spectrin SH3 domain-binding protein 1 Eps8 SH3 domain-binding protein e3B1 Nap1-binding protein Abl-binding protein 4 protein protein human Homo sapiens Human MAELQMLLEEEIPSGKRALIESYQNLTRVADYCENNYIQATDKRKALEETKAYTTQSLASVAYQINALANNVLQLLDIQASQLRRMESSINHISQTVDIHKEKVARREIGILTTNKNTSRTHKIIAPANMERPVRYIRKPIDYTVLDDVGHGVKWLKAKHGNNQPARTGTLSRTNPPTQKPPSPPMSGRGTLGRNTPYKTLEPVKPPTVPNDYMTSPARLGSQHSPGRTASLNQRPRTHSGSSGGSGSRENSGSSSIGIPIAVPTPSPPTIGPENISVPPPSGAPPAPPLAPLLPVSTVIAAPGSAPGSQYGTMTRQISRHNSTTSSTSSGGYRRTPSVTAQFSAQPHVNGGPLYSQNSISIAPPPPPMPQLTPQIPLTGFVARVQENIADSPTPPPPPPPDDIPMFDDSPPPPPPPPVDYEDEEAAVVQYNDPYADGDPAWAPKNYIEKVVAIYDYTKDKDDELSFMEGAIIYVIKKNDDGWYEGVCNRVTGLFPGNYVESIMHYTD 2D76F305934127CB ncf1_human Neutrophil cytosol factor 1 NCF1 Neutrophil NADPH oxidase factor 1 47 kDa neutrophil oxidase factor p47-phox NCF-47K 47 kDa autosomal chronic granulomatous disease protein Nox-organizing protein 2 Nox organizer 2 SH3 and PX domain-containing protein 1A NOXO2 SH3PXD1A protein protein human Homo sapiens Human MGDTFIRHIALLGFEKRFVPSQHYVYMFLVKWQDLSEKVVYRRFTEIYEFHKTLKEMFPIEAGAINPENRIIPHLPAPKWFDGQRAAENRQGTLTEYCSTLMSLPTKISRCPHLLDFFKVRPDDLKLPTDNQTKKPETYLMPKDGKSTATDITGPIILQTYRAIANYEKTSGSEMALSTGDVVEVVEKSESGWWFCQMKAKRGWIPASFLEPLDSPDETEDPEPNYAGEPYVAIKAYTAVEGDEVSLLEGEAVEVIHKLLDGWWVIRKDDVTGYFPSMYLQKSGQDVSQAQRQIKRGAPPRRSSIRNAHSIHQRSRKRLSQDAYRRNSVRFLQQRRRQARPGPQSPGSPLEEERQTQRSKPQPAVPPRPSADLILNRCSESTKRKLASAV 3D91EDC99A1B6417 abi1-ncf1-1 4 6 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey physical association physical association abi1-ncf1-2 3 5 unspecified role unspecified role prey prey 35s radiolabel radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 6 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Direct interaction of abi1 with p47(1-298) and, to a lesser extent, full-length p47phox abi1-ncf3-3 2 6 unspecified role unspecified role bait bait human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 5 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association abi1-ncf1-3 1 6 unspecified role unspecified role bait bait sh3-sh3 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey physical association physical association abi1-ncf1-4 1 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait P14598:p.[Ser303Asp;Ser304Asp;Ser328Asp] mutation mutation certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D Wed May 02 16:14:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser303asp,ser304asp,ser328asp' Tue Jun 30 18:19:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser303Asp;Ser304Asp;Ser328Asp]' physical association physical association Full-length p47(S303,304,328D) mutant mimics phosphorylation of three critical serines, and appears to induce unmasking of the tandem SH3 regions where ABI-1 binds