IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A serine protease, HtrA2, is released from the mitochondria and interacts with XIAP, inducing cell death. A serine protease, HtrA2, is released from the mitochondria and interacts with XIAP, inducing cell death. Mol. Cell (1097-2765) 2001 imex curation Suzuki Y., Imai Y., Nakayama H., Takahashi K., Takio K., Takahashi R. Apoptosis - Interactions involving proteins with a function related to apoptosis Interaction depicted in figure 4 (protease assay) was not included because the origin of beta-casein could not be stated. ryosuke@brain.riken.go.jp 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag Suzuki Y., Imai Y., Nakayama H., Takahashi K., Takio K., Takahashi R. Apoptosis - Interactions involving proteins with a function related to apoptosis Interaction depicted in figure 4 (protease assay) was not included because the origin of beta-casein could not be stated. ryosuke@brain.riken.go.jp Accepted 2011-NOV-16 by ORCHARD Mol. Cell (1097-2765) 2001 Only protein-protein interactions imex curation A serine protease, HtrA2, is released from the mitochondria and interacts with XIAP, inducing cell death. A serine protease, HtrA2, is released from the mitochondria and interacts with XIAP, inducing cell death. Mol. Cell (1097-2765) 2001 imex curation Suzuki Y., Imai Y., Nakayama H., Takahashi K., Takio K., Takahashi R. Apoptosis - Interactions involving proteins with a function related to apoptosis Interaction depicted in figure 4 (protease assay) was not included because the origin of beta-casein could not be stated. ryosuke@brain.riken.go.jp 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Suzuki Y., Imai Y., Nakayama H., Takahashi K., Takio K., Takahashi R. Apoptosis - Interactions involving proteins with a function related to apoptosis Interaction depicted in figure 4 (protease assay) was not included because the origin of beta-casein could not be stated. ryosuke@brain.riken.go.jp Accepted 2011-NOV-16 by ORCHARD 21-Dec-2011: Contacted by JYOTI. Mol. Cell (1097-2765) 2001 Only protein-protein interactions imex curation A serine protease, HtrA2, is released from the mitochondria and interacts with XIAP, inducing cell death. A serine protease, HtrA2, is released from the mitochondria and interacts with XIAP, inducing cell death. Mol. Cell (1097-2765) 2001 imex curation Suzuki Y., Imai Y., Nakayama H., Takahashi K., Takio K., Takahashi R. Apoptosis - Interactions involving proteins with a function related to apoptosis Interaction depicted in figure 4 (protease assay) was not included because the origin of beta-casein could not be stated. ryosuke@brain.riken.go.jp 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Suzuki Y., Imai Y., Nakayama H., Takahashi K., Takio K., Takahashi R. Apoptosis - Interactions involving proteins with a function related to apoptosis Interaction depicted in figure 4 (protease assay) was not included because the origin of beta-casein could not be stated. ryosuke@brain.riken.go.jp Accepted 2011-NOV-16 by ORCHARD 21-Dec-2011: Contacted by JYOTI. Mol. Cell (1097-2765) 2001 Only protein-protein interactions imex curation A serine protease, HtrA2, is released from the mitochondria and interacts with XIAP, inducing cell death. A serine protease, HtrA2, is released from the mitochondria and interacts with XIAP, inducing cell death. Mol. Cell (1097-2765) 2001 imex curation Suzuki Y., Imai Y., Nakayama H., Takahashi K., Takio K., Takahashi R. Apoptosis - Interactions involving proteins with a function related to apoptosis Interaction depicted in figure 4 (protease assay) was not included because the origin of beta-casein could not be stated. ryosuke@brain.riken.go.jp 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Suzuki Y., Imai Y., Nakayama H., Takahashi K., Takio K., Takahashi R. Apoptosis - Interactions involving proteins with a function related to apoptosis Interaction depicted in figure 4 (protease assay) was not included because the origin of beta-casein could not be stated. ryosuke@brain.riken.go.jp Accepted 2011-NOV-16 by ORCHARD 21-Dec-2011: Contacted by JYOTI. Mol. Cell (1097-2765) 2001 Only protein-protein interactions imex curation xiap_human E3 ubiquitin-protein ligase XIAP Inhibitor of apoptosis protein 3 X-linked inhibitor of apoptosis protein IAP-like protein XIAP API3 IAP3 BIRC4 Baculoviral IAP repeat-containing protein 4 RING-type E3 ubiquitin transferase XIAP protein protein human Homo sapiens Human MTFNSFEGSKTCVPADINKEEEFVEEFNRLKTFANFPSGSPVSASTLARAGFLYTGEGDTVRCFSCHAAVDRWQYGDSAVGRHRKVSPNCRFINGFYLENSATQSTNSGIQNGQYKVENYLGSRDHFALDRPSETHADYLLRTGQVVDISDTIYPRNPAMYSEEARLKSFQNWPDYAHLTPRELASAGLYYTGIGDQVQCFCCGGKLKNWEPCDRAWSEHRRHFPNCFFVLGRNLNIRSESDAVSSDRNFPNSTNLPRNPSMADYEARIFTFGTWIYSVNKEQLARAGFYALGEGDKVKCFHCGGGLTDWKPSEDPWEQHAKWYPGCKYLLEQKGQEYINNIHLTHSLEECLVRTTEKTPSLTRRIDDTIFQNPMVQEAIRMGFSFKDIKKIMEEKIQISGSNYKSLEVLVADLVNAQKDSMQDESSQTSLQKEISTEEQLRRLQEEKLCKICMDRNIAIVFVPCGHLVTCKQCAEAVDKCPMCYTVITFKQKIFMS DIP protein P98170 original sequence version: 168 DIP protein P98170 original sequence version: 118 DIP protein P98170 original sequence version: 153 9D394C16D45EB635 birc3_human Baculoviral IAP repeat-containing protein 3 Inhibitor of apoptosis protein 1 TNFR2-TRAF-signaling complex protein 1 IAP homolog C Apoptosis inhibitor 2 RING finger protein 49 BIRC3 API2 MIHC RNF49 RING-type E3 ubiquitin transferase BIRC3 Cellular inhibitor of apoptosis 2 protein protein human Homo sapiens Human MNIVENSIFLSNLMKSANTFELKYDLSCELYRMSTYSTFPAGVPVSERSLARAGFYYTGVNDKVKCFCCGLMLDNWKRGDSPTEKHKKLYPSCRFVQSLNSVNNLEATSQPTFPSSVTNSTHSLLPGTENSGYFRGSYSNSPSNPVNSRANQDFSALMRSSYHCAMNNENARLLTFQTWPLTFLSPTDLAKAGFYYIGPGDRVACFACGGKLSNWEPKDNAMSEHLRHFPKCPFIENQLQDTSRYTVSNLSMQTHAARFKTFFNWPSSVLVNPEQLASAGFYYVGNSDDVKCFCCDGGLRCWESGDDPWVQHAKWFPRCEYLIRIKGQEFIRQVQASYPHLLEQLLSTSDSPGDENAESSIIHFEPGEDHSEDAIMMNTPVINAAVEMGFSRSLVKQTVQRKILATGENYRLVNDLVLDLLNAEDEIREEERERATEEKESNDLLLIRKNRMALFQHLTCVIPILDSLLTAGIINEQEHDVIKQKTQTSLQARELIDTILVKGNIAATVFRNSLQEAEAVLYEHLFVQQDIKYIPTEDVSDLPVEEQLRRLQEERTCKVCMDKEVSIVFIPCGHLVVCKDCAPSLRKCPICRSTIKGTVRTFLS DIP protein Q13489 original sequence version: 127 8581A00BA9AAB4A7 htra2_human Serine protease HTRA2, mitochondrial High temperature requirement protein A2 Omi stress-regulated endoprotease Serine proteinase OMI Serine protease 25 HTRA2 PRSS25 OMI protein protein human Homo sapiens Human MAAPRAGRGAGWSLRAWRALGGIRWGRRPRLTPDLRALLTSGTSDPRARVTYGTPSLWARLSVGVTEPRACLTSGTPGPRAQLTAVTPDTRTREASENSGTRSRAWLAVALGAGGAVLLLLWGGGRGPPAVLAAVPSPPPASPRSQYNFIADVVEKTAPAVVYIEILDRHPFLGREVPISNGSGFVVAADGLIVTNAHVVADRRRVRVRLLSGDTYEAVVTAVDPVADIATLRIQTKEPLPTLPLGRSADVRQGEFVVAMGSPFALQNTITSGIVSSAQRPARDLGLPQTNVEYIQTDAAIDFGNSGGPLVNLDGEVIGVNTMKVTAGISFAIPSDRLREFLHRGEKKNSSSGISGSQRRYIGVMMLTLSPSILAELQLREPSFPDVQHGVLIHKVILGSPAHRAGLRPGDVILAIGEQMVQNAEDVYEAVRTQSQLAVQIRRGRETLTLYVTPEVTE CEA955A7D0DD8C0D o43464-1 Serine protease HTRA2, mitochondrial 13B HTRA2 OMI PRSS25 High temperature requirement protein A2 Omi stress-regulated endoprotease Serine protease 25 Serine proteinase OMI protein protein human Homo sapiens Human MAAPRAGRGAGWSLRAWRALGGIRWGRRPRLTPDLRALLTSGTSDPRARVTYGTPSLWARLSVGVTEPRACLTSGTPGPRAQLTAVTPDTRTREASENSGTRSRAWLAVALGAGGAVLLLLWGGGRGPPAVLAAVPSPPPASPRSQYNFIADVVEKTAPAVVYIEILDRHPFLGREVPISNGSGFVVAADGLIVTNAHVVADRRRVRVRLLSGDTYEAVVTAVDPVADIATLRIQTKEPLPTLPLGRSADVRQGEFVVAMGSPFALQNTITSGIVSSAQRPARDLGLPQTNVEYIQTDAAIDFGNSGGPLVNLDGEVIGVNTMKVTAGISFAIPSDRLREFLHRGEKKNSSSGISGSQRRYIGVMMLTLSPSILAELQLREPSFPDVQHGVLIHKVILGSPAHRAGLRPGDVILAIGEQMVQNAEDVYEAVRTQSQLAVQIRRGRETLTLYVTPEVTE CEA955A7D0DD8C0D birc2_human Baculoviral IAP repeat-containing protein 2 Inhibitor of apoptosis protein 2 TNFR2-TRAF-signaling complex protein 2 IAP homolog B RING finger protein 48 BIRC2 API1 MIHB RNF48 RING-type E3 ubiquitin transferase BIRC2 Cellular inhibitor of apoptosis 1 protein protein human Homo sapiens Human MHKTASQRLFPGPSYQNIKSIMEDSTILSDWTNSNKQKMKYDFSCELYRMSTYSTFPAGVPVSERSLARAGFYYTGVNDKVKCFCCGLMLDNWKLGDSPIQKHKQLYPSCSFIQNLVSASLGSTSKNTSPMRNSFAHSLSPTLEHSSLFSGSYSSLSPNPLNSRAVEDISSSRTNPYSYAMSTEEARFLTYHMWPLTFLSPSELARAGFYYIGPGDRVACFACGGKLSNWEPKDDAMSEHRRHFPNCPFLENSLETLRFSISNLSMQTHAARMRTFMYWPSSVPVQPEQLASAGFYYVGRNDDVKCFCCDGGLRCWESGDDPWVEHAKWFPRCEFLIRMKGQEFVDEIQGRYPHLLEQLLSTSDTTGEENADPPIIHFGPGESSSEDAVMMNTPVVKSALEMGFNRDLVKQTVQSKILTTGENYKTVNDIVSALLNAEDEKREEEKEKQAEEMASDDLSLIRKNRMALFQQLTCVLPILDNLLKANVINKQEHDIIKQKTQIPLQARELIDTILVKGNAAANIFKNCLKEIDSTLYKNLFVDKNMKYIPTEDVSGLSLEEQLRRLQEERTCKVCMDKEVSVVFIPCGHLVVCQECAPSLRKCPICRGIIKGTVRTFLS C1778D328063586D xiap-htra2-8 1 5 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range deletion ring domain sufficient to bind sufficient binding region sufficient to bind range range range range The RING domain in xIAP has been deleted in this construct. 8 unspecified role unspecified role prey prey mature htra2 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain This form of HtrA2 lacks the first 133 aminoacids that constitute the mitochondrial localization signal and that are cut out after the protein is trasnlocated to the mithochondria. physical association physical association 1a, 1b and 1c xiap-htra2-9 3 7 unspecified role unspecified role prey prey mature htra2 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain This form of HtrA2 lacks the first 133 aminoacids that constitute the mitochondrial localization signal and that are cut out after the protein is trasnlocated to the mithochondria. In this interaction, the mature form of HtrA2 is assumed to be the interacting partner of XIAP due to its estimated weight as shown in the gel (the unprocessed form should be over 45 kDa and this one is around 36 kDa). 5 unspecified role unspecified role bait bait physical association physical association 2a xiap-htra2-10 2 7 unspecified role unspecified role prey prey mature htra2 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain This form of HtrA2 lacks the first 133 aminoacids that constitute the mitochondrial localization signal and that are cut out after the protein is trasnlocated to the mithochondria. ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 5 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 2b birc2-htra2 2 7 unspecified role unspecified role prey prey ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mature htra2 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain This form of HtrA2 lacks the first 133 aminoacids that constitute the mitochondrial localization signal and that are cut out after the protein is trasnlocated to the mithochondria. 9 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 2b birc3-htra2 2 6 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 7 unspecified role unspecified role prey prey mature htra2 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain This form of HtrA2 lacks the first 133 aminoacids that constitute the mitochondrial localization signal and that are cut out after the protein is trasnlocated to the mithochondria. ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 2b xiap-htra2-11 2 7 unspecified role unspecified role prey prey ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mature htra2 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain This form of HtrA2 lacks the first 133 aminoacids that constitute the mitochondrial localization signal and that are cut out after the protein is trasnlocated to the mithochondria. 5 unspecified role unspecified role bait bait myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 2b xiap-htra2-13 4 5 unspecified role unspecified role bait bait purified purified bir2 domain sufficient to bind sufficient binding region sufficient to bind mutation analysis mutation analysis range range range range bir3 domain sufficient to bind sufficient binding region sufficient to bind mutation analysis mutation analysis range range range range gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mature htra2 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain This form of HtrA2 lacks the first 133 aminoacids that constitute the mitochondrial localization signal and that are cut out after the protein is translocated to the mithochondria. O43464:p.Ala134Met mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain A M Wed May 02 14:59:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala134met' Tue Jun 30 16:52:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala134Met' ecoli Escherichia coli (strain K12) direct interaction direct interaction 3b