IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The small subunit of the mammalian mitochondrial ribosome. Identification of the full complement of ribosomal proteins present. The small subunit of the mammalian mitochondrial ribosome. Identification of the full complement of ribosomal proteins present. J. Biol. Chem. (0021-9258) 2001 imex curation Cavdar Koc E., Burkhart W., Blackburn K., Moseley A., Spremulli LL. Apoptosis - Interactions involving proteins with a function related to apoptosis Bovine mitochondria and 28 S subunits were isolated from liver tissue and the 28 S subunits were collected by centrifugation at 48,000 rpm for 6 h in a Beckman Type-50 rotor. For analysis of individual proteins the complex was separated on two-dimensional gels followed by isolation of distinct protein spots which were trypsin digested or directly digest by trypsin of fractions from the centrifuged lysate. Protein identification was determined by analysis ogf the digested proteins using LC-MS-MS. linda_spremulli@unc.edu 01-Feb-2008: Contacted by JYOTI. Only protein-protein interactions bovin-liver Bos taurus liver liver liver density sedimentation cosedimentation through density gradient density sedimentation de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Cavdar Koc E., Burkhart W., Blackburn K., Moseley A., Spremulli LL. Apoptosis - Interactions involving proteins with a function related to apoptosis Bovine mitochondria and 28 S subunits were isolated from liver tissue and the 28 S subunits were collected by centrifugation at 48,000 rpm for 6 h in a Beckman Type-50 rotor. For analysis of individual proteins the complex was separated on two-dimensional gels followed by isolation of distinct protein spots which were trypsin digested or directly digest by trypsin of fractions from the centrifuged lysate. Protein identification was determined by analysis ogf the digested proteins using LC-MS-MS. linda_spremulli@unc.edu Accepted 2008-FEB-9 by JYOTI. 01-Feb-2008: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2001 Only protein-protein interactions imex curation rt31_bovin 28S ribosomal protein S31, mitochondrial MRPS31 protein protein bovin Bos taurus (Bovine) Bovine MFPRVSAVLPFRPLSRLPLCSAGPEASAATVVPLASPHGTVRTKCNIQRYFGTNSVIYSKKDDKSVPACEISKETENQGSTKENKKKDLVNIIKGMKVELSTVNVQTTKPPNRGQLKSLEAAIRRLQKSPEDAPQKSKSLSPELVAAATAVADSLPFDKQTTKSELLRQLRQHEEDSKAQKDGEKPKISFSNIISDMKVARSSTARASTRPVHQIQFDEGADDFVDREETADLRKRFRKNIFKGKRLNIFELKPVTEEAPETEAAPSLWDVEFAKQLAAVTEQPFQNGFEEMIQWTKEGKLWEFPINNEAGFDDDGSEFHEHIFLDKYLEGFPKQGPIRHFMELVTCGLSKNPYLSVKQKVEHIEWFRNYFNEKQDILKESGINFS 79EB2B7CB9205514 rt17_bovin 28S ribosomal protein S17, mitochondrial MRPS17 RPMS17 protein protein bovin Bos taurus (Bovine) Bovine MSVVRSSVHAKWIVGKVIGTAMQKTAKVRVTRLVLDPYLLKYFNKRKTYFAHDALQQCTVGDIVLLKALPVPRTKHVKHELAEIVFKVGQVVDPVTGKRCAGTTYLESPVDLETTPLAKNLEELSLSTTQ B391C12CE0B764A5 rt28_bovin 28S ribosomal protein S28, mitochondrial MRPS28 protein protein bovin Bos taurus (Bovine) Bovine MAALCRTRAVTAESHFLRVFLFSRSCRGAGTESGSGSESSNSTEPRPRPGGFASALERHSELQRKAELARTRGSPKNVESFASMLRHSPLTQMGPAKDKIVIGRIFHIVENDLYIDFGGKFHCVCKRPEVDGEKYQKGTRVRLRLLDLELTSRFLGATTDTTILEAEAVLLGLQESKDSKSKEERRENK 2BA7AD808EA440C4 rt18c_bovin 28S ribosomal protein S18c, mitochondrial MRPS18C 28S ribosomal protein S18-1, mitochondrial protein protein bovin Bos taurus (Bovine) Bovine MAAVLGLCGGLGKRKFTRFPTAFVCLTNSGTRAVLWRSCSQCKQVTSSEDLPIPMENPYKEPLKKCILCEKRVDYKNVQLLSQFISPFTGCIYGRHITGLCGKKQREITKAIKRAQILGFMPVTYKDPAYLKDPKVCNIRYRE 94B8CE6620110CC8 rt29_bovin 28S ribosomal protein S29, mitochondrial DAP3 MRPS29 Death-associated protein 3 protein protein bovin Bos taurus (Bovine) Bovine MLKGMTRLVSRVHKLDPGRFSHLGTQAPQCPVAHLDNQVPTERTRAISRTLENDPAKHGEQHVGQHYNISIQELKTVFPHGLPPRFVMQVKTFNEACLMVRKPALELLHYLKNTNFAHPAVRYVLYGEKGTGKTLSLCHIIHFCAKQDWLILHIPDAHLWVKNCRDLLQSTYNKQRFDQPLEASIWLKNFKTANERFLSQIKVQDKYVWNKRESTEKGSPLAEVVEQGIMRVRNATDAVGIVLKELKRQSSLGVFRLLVAVDGVNALWGRTTLKREDKSPITPEELALIYNLRKMVKNDWQGGAIVLTVSQTGSLFKPRKAYLPQELLGKEGFDTLDPFIPILVSNYNPKEFEGCIQYYLENNWLQHEKAHTEEGKKELLFLSNRNPGLLERLCAYL D4EA91BDB6A5FACB rt34_bovin 28S ribosomal protein S34, mitochondrial MRPS34 protein protein bovin Bos taurus (Bovine) Bovine MARRKVRPRLIAELARRVRALREQRERPRDSVRYALDYETLIRPHSGRKLPLRAWVDVRRESRLLQLLGRLPFFGLGRLVTRKSWLWQHDEPCYWRLTRVRPDYTAQNLDHGKAWGILTFKGKTESEAREIEQVMHHDWRLVPKHEEAAFTSFTPAPEETPRPVPYPPLLRAMILAERQKNGDPSTEEPMLSLERIRTDPWDYPENQEAKKKTKGTAV 05E128159BB72D39 rt15_bovin 28S ribosomal protein S15, mitochondrial MRPS15 RPMS15 protein protein bovin Bos taurus (Bovine) Bovine MLRAAWRALSSIRTQAVTQPPVLGLPGGGCAKLLSVQRDLPSSLILQAARGYATQKPVQQSQEDDPPPSMLLKDYQNVPGIEKVDDVVKRLISLEMANKKEMLKIKKEQLMSKVVENPKDTSSLEAQIVALTVKIRSYEEHMQKHQKDKAHKRYLLMSIDKRQKMLKNLRKTNYPVFEKTCKELGIEYTFPPPYHRKIHRRLATKKALCIRVFQEVQKLKKQKRALKTAAAAAQKQGQRNPESPSEARSEAIKETQ 63D6EB64776E2740 rt30_bovin 28S ribosomal protein S30, mitochondrial MRPS30 protein protein bovin Bos taurus (Bovine) Bovine MAATRCWRFVLRSPGLSLHTAAEATVTAPEVTGSDVKAAPVARYPPIVASLTADSKAARQRRVERWQATVHAAKSVDEKLRILTKMQFMKYVVYPQTFALNADRWYQSFTKTVFLSGLPPPQAQPDREPAQVVDLAALRAAVCDCLLQEHFFLRRKKRAPIYQERYAVASPFLDQLVPSLTGLLSAYNPVLAAAALDCNRPVHFYWLRGEEIIPGGHRKGRVDAVRYQINDKPHNQIRISRQLPEFVPLDYSVPVEVPVKNCKPDKLPLFKRQYENAIFIGTKTADPLCYGHTQFHLLPDKLKRERLLKQNCADQIEVIFRANAIASLFAWTGAQAMYQGFWSEADVTRPFVSQGVITDGKYFSFFCYQLNTLALTAQADQNNPRKNICWGTQSMPLYETIEDNDVKGFNDDVLLQLVHFLLNRPEEDKAQLLVN AEB490EB7459F65D rt11_bovin 28S ribosomal protein S11, mitochondrial MRPS11 protein protein bovin Bos taurus (Bovine) Bovine MQVLRNSGSWLLRSWAWPPTTRVVAGVPAPTIHMSAQQMQDAAAKEEVEKAETPAPAPSRSSFSIYPPIPGQESSLRWAGKKFEEIPIAHIKASYNNTQIHVVSAAHQPLARASCGTEGFRNAKKGTGIAAQTAGIAAAAKATGKGVTHVRVVVKGLGPGRLSAIKGLTMGGLEVISITDNTPIPHNGCRPRKARRL E543BD746FBBA472 rt36_bovin 28S ribosomal protein S36, mitochondrial MRPS36 Alpha-ketoglutarate dehydrogenase component 4 KGD4 protein protein bovin Bos taurus (Bovine) Bovine MMGSKMASASRVVQVVKPHTPLIRFPDRRDNPKPNVSEVLRSAGLPSHTSSISQHSKGSKSPDWLMHQGPPDTAEMIKTLPQKYRRKLVSQEEIEFIQRGGPE B79BE89385D89E2C rt33_bovin 28S ribosomal protein S33, mitochondrial MRPS33 protein protein bovin Bos taurus (Bovine) Bovine MSSLSEYALRMSRLSARLFSEVARPTDSKSMKVVKLFSEQPLAKRKETYDWYPNHNTYFALMGILRSVGLYRDEHQDFKDEQLRLKKLRGKVKPRKGEGKRAAKKK 0D760E8956EFC51F rt18a_bovin 28S ribosomal protein S18a, mitochondrial MRPS18A 28S ribosomal protein S18-3, mitochondrial protein protein bovin Bos taurus (Bovine) Bovine MAALNVLVSGCGRFLRGLLTGPTVTSWARPPARGFREVVEIQEGKTTIIEGRITGTPKESPNPPNPSGQCPICRWNLKHKYSYEDVLLLSQFIRPHGGMLPRSITGLCQEEHRKIEECVKMAHRAGLLPNHRPKLPEGFFPKTRPRLNRYLTRWSPRSVKPIYNKGHRWNKVRMAVGSPLLKDNVSYTGRPLVLYH 0CAA7B308EAD5CF1 rt21_bovin 28S ribosomal protein S21, mitochondrial MRPS21 RPMS21 protein protein bovin Bos taurus (Bovine) Bovine MAKHLKFIARTVMVQEGNVEGAYRTLNRILTMDGLIEDIKRRRYYEKPCRRRQRESYETCRRIYNMEMARKINFLMRKNRADPWQGC D28696851F9150E7 rt06_bovin 28S ribosomal protein S6, mitochondrial MRPS6 RPMS6 protein protein bovin Bos taurus (Bovine) Bovine MPRYELALILKAMQRPETAAALKRTLEALMDRGAVVRSLENLGERTLPYKMSAHSQRHTRGGYFLVDFYAPTTTVASIMEHLSRDIDVIRPNVVKHPLTQEVKECEGIVPVPLEEKLYSTKKRK A85114B7B8255657 rt18b_bovin 28S ribosomal protein S18b, mitochondrial MRPS18B 28S ribosomal protein S18-2, mitochondrial protein protein bovin Bos taurus (Bovine) Bovine MAASVLNVLLRRLPYFSPFRGAYGVQVPLQTLCTKAPPEDDSLPPIPVSPYEDEPWKYLDSEEYHNRNGSRPVWADYRRNHKGGIPPQRTRKMCIRGNKVAGNPCPICRDQKLHVDFRNVKLLKQFVCAHTGIIFHAPYTGVCMKQHKKLTQAIQKARDHGLLSYHIPQVEPRDLDFSTSHGAVSATPPAPTLVSGDPWYPWYSWKQPPERELSRLRRLYQGHLREESGPPPESMPKVPLTAPNEATSTEQAGPQSAL EC704F50FB1E0CFE rt16_bovin 28S ribosomal protein S16, mitochondrial MRPS16 RPMS16 protein protein bovin Bos taurus (Bovine) Bovine MVQLTTVLCKAYRGGHLTIRLALGGCTNRPFYRIVAAHNKCPRDGRFVEQLGSYDPMPNSHGEKLVALNLDRIRHWIGCGAHLSKPVEKLLGLSGFFPLHPMVITNAERLRRKRAQEVLLAAQKTDTEATETKEN 96264C9886ED1E6B rm42_bovin 39S ribosomal protein L42, mitochondrial MRPL42 MRPS32 28S ribosomal protein S32, mitochondrial protein protein bovin Bos taurus (Bovine) Bovine MALAAVKWAISSRTMLKHLFPVENGALYCVGHKSTYSSLPDDYNCKVELALTSDARTIVCYHPSVDIPYEHTKPIPRPDPVHNNEETHDLVLKTRLEEKSEHLEQGPMIEQLSKMFFTTKHRWYPRGQYHRRRRKLNPPKDR 56C47A9699226C26 rt02_bovin 28S ribosomal protein S2, mitochondrial MRPS2 protein protein bovin Bos taurus (Bovine) Bovine MATGAVLPRLLGAGVRAAPRGRAAQRGRTLGSAAAAAAREPERDSDRSARILSEPLKHSDFFNVKELFSVRSLFNARVHLGHKAGCRHRFMEPYIFGSRLGQDIIDLEQTATHLQLALNFTAHVAFRGGIILFVSRARQFSHLIESTARSCGEYAHTRYFKGGLLTNAPLLLGARVRLPDLIIFLHTLNNVFEPHVAVRDAAKMSIPTVGVVDTNCNPCLITYPVPGNDDSPPAVQLFCQLFQTAVTRAKEKRRQLEALYRLQGAPGPHPANPAAPGAPSPGAQAQLGMGHSP 99DF74A062564211 rt1-rt02 1 5 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component 17 unspecified role unspecified role neutral component neutral component 18 unspecified role unspecified role neutral component neutral component 15 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component 19 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component 16 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component 14 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component 2 unspecified role unspecified role neutral component neutral component physical association physical association Table 1