IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Identification of a caspase-2 isoform that behaves as an endogenous inhibitor of the caspase cascade. Identification of a caspase-2 isoform that behaves as an endogenous inhibitor of the caspase cascade. Cancer Res. (0008-5472) 2000 Droin N., Beauchemin M., Solary E., Bertrand R. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Source of RAIDD (CRADD) taken from PMID 8985253. (A paper by the donor of the construct. The sequence given in the paper is identical to human RAIDD/CRADD). richard.bertrand@umontreal.ca 25-Jun-2008: Contacted by JYOTI. davet human-namalwa Homo sapiens namalwa Human Burkitt's Lymphoma anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Droin N., Beauchemin M., Solary E., Bertrand R. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of RAIDD (CRADD) taken from PMID 8985253. (A paper by the donor of the construct. The sequence given in the paper is identical to human RAIDD/CRADD). Source of RAIDD (CRADD) taken from PMID 8985253. (A paper by the donor of the construct. The sequence given in the paper is identical to human RAIDD/CRADD). richard.bertrand@umontreal.ca Accepted 2008-JUN-13 by DAVET. 25-Jun-2008: Contacted by JYOTI. Cancer Res. (0008-5472) 2000 davet Identification of a caspase-2 isoform that behaves as an endogenous inhibitor of the caspase cascade. Identification of a caspase-2 isoform that behaves as an endogenous inhibitor of the caspase cascade. Cancer Res. (0008-5472) 2000 Droin N., Beauchemin M., Solary E., Bertrand R. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Source of RAIDD (CRADD) taken from PMID 8985253. (A paper by the donor of the construct. The sequence given in the paper is identical to human RAIDD/CRADD). richard.bertrand@umontreal.ca 25-Jun-2008: Contacted by JYOTI. davet yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Droin N., Beauchemin M., Solary E., Bertrand R. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of RAIDD (CRADD) taken from PMID 8985253. (A paper by the donor of the construct. The sequence given in the paper is identical to human RAIDD/CRADD). Source of RAIDD (CRADD) taken from PMID 8985253. (A paper by the donor of the construct. The sequence given in the paper is identical to human RAIDD/CRADD). Source of RAIDD (CRADD) taken from PMID 8985253. (A paper by the donor of the construct. The sequence given in the paper is identical to human RAIDD/CRADD). richard.bertrand@umontreal.ca Accepted 2008-JUN-13 by DAVET. Cancer Res. (0008-5472) 2000 davet Identification of a caspase-2 isoform that behaves as an endogenous inhibitor of the caspase cascade. Identification of a caspase-2 isoform that behaves as an endogenous inhibitor of the caspase cascade. Cancer Res. (0008-5472) 2000 Droin N., Beauchemin M., Solary E., Bertrand R. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Source of RAIDD (CRADD) taken from PMID 8985253. (A paper by the donor of the construct. The sequence given in the paper is identical to human RAIDD/CRADD). richard.bertrand@umontreal.ca 25-Jun-2008: Contacted by JYOTI. davet human-namalwa Homo sapiens namalwa Human Burkitt's Lymphoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Droin N., Beauchemin M., Solary E., Bertrand R. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of RAIDD (CRADD) taken from PMID 8985253. (A paper by the donor of the construct. The sequence given in the paper is identical to human RAIDD/CRADD). Source of RAIDD (CRADD) taken from PMID 8985253. (A paper by the donor of the construct. The sequence given in the paper is identical to human RAIDD/CRADD). richard.bertrand@umontreal.ca Accepted 2008-JUN-13 by DAVET. 25-Jun-2008: Contacted by JYOTI. Cancer Res. (0008-5472) 2000 davet casp2_human Caspase-2 Protease ICH-1 Neural precursor cell expressed developmentally down-regulated protein 2 CASP2 ICH1 NEDD2 protein protein human Homo sapiens Human MAAPSAGSWSTFQHKELMAADRGRRILGVCGMHPHHQETLKKNRVVLAKQLLLSELLEHLLEKDIITLEMRELIQAKVGSFSQNVELLNLLPKRGPQAFDAFCEALRETKQGHLEDMLLTTLSGLQHVLPPLSCDYDLSLPFPVCESCPLYKKLRLSTDTVEHSLDNKDGPVCLQVKPCTPEFYQTHFQLAYRLQSRPRGLALVLSNVHFTGEKELEFRSGGDVDHSTLVTLFKLLGYDVHVLCDQTAQEMQEKLQNFAQLPAHRVTDSCIVALLSHGVEGAIYGVDGKLLQLQEVFQLFDNANCPSLQNKPKMFFIQACRGDETDRGVDQQDGKNHAGSPGCEESDAGKEKLPKMRLPTRSDMICGYACLKGTAAMRNTKRGSWYIEALAQVFSERACDMHVADMLVKVNALIKDREGYAPGTEFHRCKEMSEYCSTLCRHLYLFPGHPPT 6EF0ED05EF808385 cradd_human Death domain-containing protein CRADD Caspase and RIP adapter with death domain RIP-associated protein with a death domain CRADD RAIDD protein protein human Homo sapiens Human MEARDKQVLRSLRLELGAEVLVEGLVLQYLYQEGILTENHIQEINAQTTGLRKTMLLLDILPSRGPKAFDTFLDSLQEFPWVREKLKKAREEAMTDLPAGDRLTGIPSHILNSSPSDRQINQLAQRLGPEWEPMVLSLGLSQTDIYRCKANHPHNVQSQVVEAFIRWRQRFGKQATFQSLHNGLRAVEVDPSLLLHMLE 3437CC612C85E402 p42575-1 Caspase-2 CASP2 ICH1 NEDD2 Neural precursor cell expressed developmentally down-regulated protein 2 Protease ICH-1 ICH-1L protein protein human Homo sapiens Human MAAPSAGSWSTFQHKELMAADRGRRILGVCGMHPHHQETLKKNRVVLAKQLLLSELLEHLLEKDIITLEMRELIQAKVGSFSQNVELLNLLPKRGPQAFDAFCEALRETKQGHLEDMLLTTLSGLQHVLPPLSCDYDLSLPFPVCESCPLYKKLRLSTDTVEHSLDNKDGPVCLQVKPCTPEFYQTHFQLAYRLQSRPRGLALVLSNVHFTGEKELEFRSGGDVDHSTLVTLFKLLGYDVHVLCDQTAQEMQEKLQNFAQLPAHRVTDSCIVALLSHGVEGAIYGVDGKLLQLQEVFQLFDNANCPSLQNKPKMFFIQACRGDETDRGVDQQDGKNHAGSPGCEESDAGKEKLPKMRLPTRSDMICGYACLKGTAAMRNTKRGSWYIEALAQVFSERACDMHVADMLVKVNALIKDREGYAPGTEFHRCKEMSEYCSTLCRHLYLFPGHPPT Acts as a positive regulator of apoptosis. 6EF0ED05EF808385 casp2-cradd 2 4 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey physical association physical association 3 A In a yeast 2-hybrid assay, caspase-2L-Pro interacted with both procaspase-2L and RAIDD/CRADD, The authors state that the caspase-2L-Pro/procaspase-2L interaction appeared weaker than the caspase-2L-Pro/RAIDD (CRADD) interaction. casp2-casp2 2 4 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey physical association physical association 3 A In a yeast 2-hybrid assay, caspase-2L-Pro interacted with both procaspase-2L and RAIDD/CRADD, The authors state that the caspase-2L-Pro/procaspase-2L interaction appeared weaker than the caspase-2L-Pro/RAIDD (CRADD) interaction. casp2-cradd-7 1 4 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3 B The caspase-2L-Pro/procaspase-2L interaction appeared weaker than the caspase-2L-Pro/RAIDD interaction, both in the yeast 2-hybrid assay and in cotransfected cells The authors state that the caspase-2L-Pro/procaspase-2L interaction appeared weaker than the caspase-2L-Pro/RAIDD (CRADD) interaction. P78560; P42575 transfection casp2-casp2-1 3 6 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3 C The caspase-2L-Pro/procaspase-2L interaction appeared weaker than the caspase-2L-Pro/RAIDD interaction, both in the yeast 2-hybrid assay and in cotransfected cells The authors state that the caspase-2L-Pro/procaspase-2L interaction appeared weaker than the caspase-2L-Pro/RAIDD (CRADD) interaction. P42575; P42575-1 transfection