IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ AXL Mediates Resistance to PI3Kα Inhibition by Activating the EGFR/PKC/mTOR Axis in Head and Neck and Esophageal Squamous Cell Carcinomas. AXL Mediates Resistance to PI3Kα Inhibition by Activating the EGFR/PKC/mTOR Axis in Head and Neck and Esophageal Squamous Cell Carcinomas. Cancer cell 2015 imex curation Elkabets M., Pazarentzos E., Juric D., Sheng Q., Pelossof RA., Brook S., Benzaken AO., Rodon J., Morse N., Yan JJ., Liu M., Das R., Chen Y., Tam A., Wang H., Liang J., Gurski JM., Kerr DA., Rosell R., Teixidó C., Huang A., Ghossein RA., Rosen N., Bivona TG., Scaltriti M., Baselga J. Affinomics - Interactions curated for the Affinomics consortium. Cancer - Interactions investigated in the context of cancer scaltrim@mskcc.org,baselgaj@mskcc.org Only protein-protein interactions imex curation human-cal33 CAL 33-Human tongue squamous cell carcinoma cell line Human cal33 CAL 33-Human tongue squamous cell carcinoma cell line DSMZ no.: ACC 447 pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Elkabets M., Pazarentzos E., Juric D., Sheng Q., Pelossof RA., Brook S., Benzaken AO., Rodon J., Morse N., Yan JJ., Liu M., Das R., Chen Y., Tam A., Wang H., Liang J., Gurski JM., Kerr DA., Rosell R., Teixidó C., Huang A., Ghossein RA., Rosen N., Bivona TG., Scaltriti M., Baselga J. Affinomics - Interactions curated for the Affinomics consortium. CAL33-R cells-a BYL719 (PIK3CA inhibitor) resistant cell line scaltrim@mskcc.org,baselgaj@mskcc.org Accepted 2015-JUN-25 by ORCHARD Cancer cell 2015 Only protein-protein interactions imex curation imex curation AXL Mediates Resistance to PI3Kα Inhibition by Activating the EGFR/PKC/mTOR Axis in Head and Neck and Esophageal Squamous Cell Carcinomas. AXL Mediates Resistance to PI3Kα Inhibition by Activating the EGFR/PKC/mTOR Axis in Head and Neck and Esophageal Squamous Cell Carcinomas. Cancer cell 2015 imex curation Elkabets M., Pazarentzos E., Juric D., Sheng Q., Pelossof RA., Brook S., Benzaken AO., Rodon J., Morse N., Yan JJ., Liu M., Das R., Chen Y., Tam A., Wang H., Liang J., Gurski JM., Kerr DA., Rosell R., Teixidó C., Huang A., Ghossein RA., Rosen N., Bivona TG., Scaltriti M., Baselga J. Affinomics - Interactions curated for the Affinomics consortium. Cancer - Interactions investigated in the context of cancer scaltrim@mskcc.org,baselgaj@mskcc.org Only protein-protein interactions imex curation human-kyse-180 KYSE-180 Esophageal squamous cell Carcinoma Cell Line Human kyse-180 KYSE-180 Esophageal squamous cell Carcinoma Cell Line DSMZ no.: ACC 379 pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Elkabets M., Pazarentzos E., Juric D., Sheng Q., Pelossof RA., Brook S., Benzaken AO., Rodon J., Morse N., Yan JJ., Liu M., Das R., Chen Y., Tam A., Wang H., Liang J., Gurski JM., Kerr DA., Rosell R., Teixidó C., Huang A., Ghossein RA., Rosen N., Bivona TG., Scaltriti M., Baselga J. Affinomics - Interactions curated for the Affinomics consortium. KYSE-180 R cells-a BYL719 (PIK3CA inhibitor) resistant cell line scaltrim@mskcc.org,baselgaj@mskcc.org Accepted 2015-JUN-25 by ORCHARD Cancer cell 2015 Only protein-protein interactions imex curation imex curation AXL Mediates Resistance to PI3Kα Inhibition by Activating the EGFR/PKC/mTOR Axis in Head and Neck and Esophageal Squamous Cell Carcinomas. AXL Mediates Resistance to PI3Kα Inhibition by Activating the EGFR/PKC/mTOR Axis in Head and Neck and Esophageal Squamous Cell Carcinomas. Cancer cell 2015 imex curation Elkabets M., Pazarentzos E., Juric D., Sheng Q., Pelossof RA., Brook S., Benzaken AO., Rodon J., Morse N., Yan JJ., Liu M., Das R., Chen Y., Tam A., Wang H., Liang J., Gurski JM., Kerr DA., Rosell R., Teixidó C., Huang A., Ghossein RA., Rosen N., Bivona TG., Scaltriti M., Baselga J. Affinomics - Interactions curated for the Affinomics consortium. Cancer - Interactions investigated in the context of cancer scaltrim@mskcc.org,baselgaj@mskcc.org Only protein-protein interactions imex curation human-cal33 CAL 33-Human tongue squamous cell carcinoma cell line Human cal33 CAL 33-Human tongue squamous cell carcinoma cell line DSMZ no.: ACC 447 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Elkabets M., Pazarentzos E., Juric D., Sheng Q., Pelossof RA., Brook S., Benzaken AO., Rodon J., Morse N., Yan JJ., Liu M., Das R., Chen Y., Tam A., Wang H., Liang J., Gurski JM., Kerr DA., Rosell R., Teixidó C., Huang A., Ghossein RA., Rosen N., Bivona TG., Scaltriti M., Baselga J. Affinomics - Interactions curated for the Affinomics consortium. CAL33-R cells-a BYL719 (PIK3CA inhibitor) resistant cell line This publication refers to various phosphorylated tyrosine residues whose presence is detected by site specific antibodies. The location of these phosphorylated tyrosine residues as has been mapped to the canonical UniProt entry for EGFR (P00533) by reference to peptide sequences listed in Chattopadhyay et, 1999, pmid: 10473558. scaltrim@mskcc.org,baselgaj@mskcc.org Accepted 2015-JUN-25 by ORCHARD Cancer cell 2015 Only protein-protein interactions imex curation imex curation AXL Mediates Resistance to PI3Kα Inhibition by Activating the EGFR/PKC/mTOR Axis in Head and Neck and Esophageal Squamous Cell Carcinomas. AXL Mediates Resistance to PI3Kα Inhibition by Activating the EGFR/PKC/mTOR Axis in Head and Neck and Esophageal Squamous Cell Carcinomas. Cancer cell 2015 imex curation Elkabets M., Pazarentzos E., Juric D., Sheng Q., Pelossof RA., Brook S., Benzaken AO., Rodon J., Morse N., Yan JJ., Liu M., Das R., Chen Y., Tam A., Wang H., Liang J., Gurski JM., Kerr DA., Rosell R., Teixidó C., Huang A., Ghossein RA., Rosen N., Bivona TG., Scaltriti M., Baselga J. Affinomics - Interactions curated for the Affinomics consortium. Cancer - Interactions investigated in the context of cancer scaltrim@mskcc.org,baselgaj@mskcc.org Only protein-protein interactions imex curation human-kyse-180 KYSE-180 Esophageal squamous cell Carcinoma Cell Line Human kyse-180 KYSE-180 Esophageal squamous cell Carcinoma Cell Line DSMZ no.: ACC 379 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Elkabets M., Pazarentzos E., Juric D., Sheng Q., Pelossof RA., Brook S., Benzaken AO., Rodon J., Morse N., Yan JJ., Liu M., Das R., Chen Y., Tam A., Wang H., Liang J., Gurski JM., Kerr DA., Rosell R., Teixidó C., Huang A., Ghossein RA., Rosen N., Bivona TG., Scaltriti M., Baselga J. Affinomics - Interactions curated for the Affinomics consortium. KYSE-180 R cells-a BYL719 (PIK3CA inhibitor) resistant cell line This publication refers to various phosphorylated tyrosine residues whose presence is detected by site specific antibodies. The location of these phosphorylated tyrosine residues as has been mapped to the canonical UniProt entry for EGFR (P00533) by reference to peptide sequences listed in Chattopadhyay et, 1999, pmid: 10473558. scaltrim@mskcc.org,baselgaj@mskcc.org Accepted 2015-JUN-25 by ORCHARD Cancer cell 2015 Only protein-protein interactions imex curation imex curation egfr_human Epidermal growth factor receptor ERBB1 EGFR Receptor tyrosine-protein kinase erbB-1 Proto-oncogene c-ErbB-1 ERBB HER1 protein protein human Homo sapiens Human MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA DIP protein P00533 original sequence version: 213 DIP protein P00533 original sequence version: 214 DIP protein P00533 original sequence version: 119 DIP protein P00533 original sequence version: 207 DIP protein P00533 original sequence version: 132 DIP protein P00533-1 original sequence version: 197 DIP protein P00533 original sequence version: 163 DIP protein P00533-1 original sequence version: 159 DIP protein P00533 original sequence version: 184 DIP protein P00533-1 original sequence version: 174 DIP protein P00533-1 original sequence version: 191 DIP protein P00533 original sequence version: 218 DIP protein P00533 original sequence version: 221 DIP protein P00533-1 original sequence version: 221 DIP protein P00533 original sequence version: 226 DIP protein P00533 original sequence version: 166 DIP protein P00533-1 original sequence version: 166 DIP protein P00533 original sequence version: 194 DIP protein P00533-1 original sequence version: 194 DIP protein P00533-1 original sequence version: 224 DIP protein P00533 original sequence version: 189 DIP protein P00533-1 original sequence version: 207 DIP protein P00533 original sequence version: 177 DIP protein P00533 original sequence version: 168 D8A2A50B4EFB6ED2 ufo_human Tyrosine-protein kinase receptor UFO UFO AXL oncogene AXL protein protein human Homo sapiens Human MAWRCPRMGRVPLAWCLALCGWACMAPRGTQAEESPFVGNPGNITGARGLTGTLRCQLQVQGEPPEVHWLRDGQILELADSTQTQVPLGEDEQDDWIVVSQLRITSLQLSDTGQYQCLVFLGHQTFVSQPGYVGLEGLPYFLEEPEDRTVAANTPFNLSCQAQGPPEPVDLLWLQDAVPLATAPGHGPQRSLHVPGLNKTSSFSCEAHNAKGVTTSRTATITVLPQQPRNLHLVSRQPTELEVAWTPGLSGIYPLTHCTLQAVLSDDGMGIQAGEPDPPEEPLTSQASVPPHQLRLGSLHPHTPYHIRVACTSSQGPSSWTHWLPVETPEGVPLGPPENISATRNGSQAFVHWQEPRAPLQGTLLGYRLAYQGQDTPEVLMDIGLRQEVTLELQGDGSVSNLTVCVAAYTAAGDGPWSLPVPLEAWRPGQAQPVHQLVKEPSTPAFSWPWWYVLLGAVVAAACVLILALFLVHRRKKETRYGEVFEPTVERGELVVRYRVRKSYSRRTTEATLNSLGISEELKEKLRDVMVDRHKVALGKTLGEGEFGAVMEGQLNQDDSILKVAVKTMKIAICTRSELEDFLSEAVCMKEFDHPNVMRLIGVCFQGSERESFPAPVVILPFMKHGDLHSFLLYSRLGDQPVYLPTQMLVKFMADIASGMEYLSTKRFIHRDLAARNCMLNENMSVCVADFGLSKKIYNGDYYRQGRIAKMPVKWIAIESLADRVYTSKSDVWSFGVTMWEIATRGQTPYPGVENSEIYDYLRQGNRLKQPADCLDGLYALMSRCWELNPQDRPSFTELREDLENTLKALPPAQEPDEILYVNMDEGGGYPEPPGAAGGADPPTQPDPKDSCSCLTAAEVHPAGRYVLCPSTTPSPAQPADRGSPAAPGQEDGA 262D3659218E3202 egfr-axl-2 3 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait N/A optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm N/A optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm physical association physical association 4H, 6C, supp 6B The cells in the resistant model (CAL33R) show increased EGFR and AXL interaction. R428 - a small molecule ATP competitor that inhibits the AXL by in this instance preventing the specific interaction between (phosphoryated) residue 1197 (1173) of EGFR with AXL. egfr-axl-1 1 6 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 4I, Supp 6C The number of AXL/EGFR complexes is significantly higher in resistant cell lines compared to the parental counterpart. R428 - a small molecule ATP competitor that inhibits the AXL by in this instance preventing the specific interaction between (phosphoryated) residue 1197 (1173) of EGFR with AXL. axl-egfr-1 2 6 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component tyr-1197 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm proximity proximity close-contact colocalization neighbourhood interaction 4I, supp 6C The number of AXL/EGFR complexes is significantly higher in resistant cell lines compared to the parental counterpart. R428 - a small molecule ATP competitor that inhibits the AXL by in this instance preventing the specific interaction between (phosphoryated) residue 1197 (1173) of EGFR with AXL. egfr-axl-3 4 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait N/A optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm N/A optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm physical association physical association 4H, Supp 6B The cells in the resistant model (CAL33R) show increased EGFR and AXL interaction. R428 - a small molecule ATP competitor that inhibits the AXL by in this instance preventing the specific interaction between (phosphoryated) residue 1197 (1173) of EGFR with AXL.