IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Sensitivity and specificity of in situ proximity ligation for protein interaction analysis in a model of steatohepatitis with Mallory-Denk bodies. Sensitivity and specificity of in situ proximity ligation for protein interaction analysis in a model of steatohepatitis with Mallory-Denk bodies. PLoS ONE 2014 imex curation Zatloukal B., Kufferath I., Thueringer A., Landegren U., Zatloukal K., Haybaeck J. Affinomics - Interactions curated for the Affinomics consortium. johannes.haybaeck@medunigraz.at 22-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions mouse-liver Mus musculus liver liver confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Zatloukal B., Kufferath I., Thueringer A., Landegren U., Zatloukal K., Haybaeck J. Affinomics - Interactions curated for the Affinomics consortium. "cytoplasmic inclusion" is the nearest GO term to Mallory Denk bodies - unfortunately the term 'cytoplasmic inclusion' in OLS refers to Lewy bodies - so not relevant. johannes.haybaeck@medunigraz.at Accepted 2015-JAN-19 by ORCHARD PLoS ONE 2014 Only protein-protein interactions imex curation Sensitivity and specificity of in situ proximity ligation for protein interaction analysis in a model of steatohepatitis with Mallory-Denk bodies. Sensitivity and specificity of in situ proximity ligation for protein interaction analysis in a model of steatohepatitis with Mallory-Denk bodies. PLoS ONE 2014 imex curation Zatloukal B., Kufferath I., Thueringer A., Landegren U., Zatloukal K., Haybaeck J. Affinomics - Interactions curated for the Affinomics consortium. johannes.haybaeck@medunigraz.at 22-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions mouse-liver Mus musculus liver liver pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Zatloukal B., Kufferath I., Thueringer A., Landegren U., Zatloukal K., Haybaeck J. Affinomics - Interactions curated for the Affinomics consortium. "cytoplasmic inclusion" is the nearest GO term to Mallory Denk bodies - unfortunately the term 'cytoplasmic inclusion' in OLS refers to Lewy bodies - so not relevant. johannes.haybaeck@medunigraz.at Accepted 2015-JAN-19 by ORCHARD PLoS ONE 2014 Only protein-protein interactions imex curation sqstm_mouse Sequestosome-1 Ubiquitin-binding protein p62 STONE14 Sqstm1 A170 STAP protein protein mouse Mus musculus Mouse MASFTVKAYLLGKEEATREIRRFSFCFSPEPEAEAQAAAGPGPCERLLSRVAVLFPTLRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRREHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHREHSKLIFPNPFGHLSDSFSHSRWLRKLKHGHFGWPGWEMGPPGNWSPRPPRAGDGRPCPTAESASAPPEDPNVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPTTPESSSTGTEDKSNTQPSSCSSEVSKPDGAGEGPAQSLTEQMKKIALESVGQPEEQMESGNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL DIP protein Q64337 original sequence version: 121 DIP protein Q64337 original sequence version: 130 ED4CCCA7741D35CA k2c8_mouse Keratin, type II cytoskeletal 8 Krt8 Krt2-8 Cytokeratin endo A Cytokeratin-8 Keratin-8 Type-II keratin Kb8 protein protein mouse Mus musculus Mouse MSIRVTQKSYKMSTSGPRAFSSRSFTSGPGARISSSSFSRVGSSSSSFRGSMGTGVGLGGFGGAGVGGITAVTVNQSLLSPLKLEVDPNIQAVRTQEKEQIKSLNNKFASFIDKVRFLEQQNKMLETKWSLLQQQKTSRSNMDNMFESYINNLRRQLEALGQEKLKLEAELGNMQGLVEDFKNKYEDEINKRTEMENEFVLIKKDVDEAYMNKVELESRLEGLTDEINFLRQIHEEEIRELQSQISDTSVVLSMDNSRSLDMDGIIAEVRAQYEDIANRSRAEAETMYQIKYEELQTLAGKHGDDLRRTKTEISEMNRNINRLQAEIEALKGQRASLEAAIADAEQRGEMAIKDAQTKLAELEAALQRAKQDMARQLREYQELMNVKLALDIEITTYRKLLEGEESRLESGMQNMSIHTKTTSGYSGGLSSSYGGLTSPGFSYGMSSFQPGFGSAGGSNTFSRTTKAVVVKKIETRDGKLVSESSDVVSK C0C79590F0C28502 k1c18_mouse Keratin, type I cytoskeletal 18 Krt18 Kerd Krt1-18 Cytokeratin-18 Keratin-18 Cytokeratin endo B protein protein mouse Mus musculus Mouse MSFTTRSTTFSTNYRSLGSVRTPSQRVRPASSAASVYAGAGGSGSRISVSRSVWGGSVGSAGLAGMGGIQTEKETMQDLNDRLASYLDKVKSLETENRRLESKIREHLEKKGPQGVRDWGHYFKIIEDLRAQIFANSVDNARIVLQIDNARLAADDFRVKYETELAMRQSVESDIHGLRKVVDDTNITRLQLETEIEALKEELLFMKKNHEEEVQGLEAQIASSGLTVEVDAPKSQDLSKIMADIRAQYEALAQKNREELDKYWSQQIEESTTVVTTKSAEIRDAETTLTELRRTLQTLEIDLDSMKNQNINLENSLGDVEARYKAQMEQLNGVLLHLESELAQTRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFSLNDALDSSNSMQTVQKTTTRKIVDGRVVSETNDTRVLRH mint When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection A67ACD6814A02118 krt8-sqstm1 1 3 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component colocalization colocalization 3A, 5C DCC -3,5-diethoxycarbonyl-1,4-dihydrocollidine (CHEBI:83605) After DDC treatment ballooned hepatocytes were present with a disrupted keratin cytoskeleton and MDBs (Mallory-Denk Bodies) involving K8, K18 and p62 were observed. A positive colocalization of p62 and K8 in krt18−/− mice was observed. krt8-sqstm1-1 1 5 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component colocalization colocalization 3B DCC -3,5-diethoxycarbonyl-1,4-dihydrocollidine (CHEBI:83605) After DDC treatment ballooned hepatocytes were present with a disrupted keratin cytoskeleton and MDBs (Mallory-Denk Bodies) involving K8, K18 and p62 were observed krt8-sqstm1-2 2 5 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 4 DCC -3,5-diethoxycarbonyl-1,4-dihydrocollidine (CHEBI:83605) After DDC treatment ballooned hepatocytes were present with a disrupted keratin cytoskeleton and MDBs (Mallory-Denk Bodies) involving K8, K18 and p62 were observed krt8-sqstm1-2 2 4 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 4, 6C,7 DCC -3,5-diethoxycarbonyl-1,4-dihydrocollidine (CHEBI:83605) After DDC treatment ballooned hepatocytes were present with a disrupted keratin cytoskeleton and MDBs (Mallory-Denk Bodies) involving K8, K18 and p62 were observed. A positive PLA result was seen in krt18−/− mice.