IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Coordinated function of WASH and exocyst complex underlies the mechanism of invadopodial matrix degradation by tumor cells Coordinated function of WASH and exocyst complex underlies the mechanism of invadopodial matrix degradation by tumor cells 2013 imex curation Monteiro P, Chavrier P, Formstecher E Affinomics - Interactions curated for the Affinomics consortium. philippe.chavrier@curie.fr, pedro.monteiro@curie.fr, eformstecher@hybrigenics.com 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Monteiro P, Chavrier P, Formstecher E Affinomics - Interactions curated for the Affinomics consortium. philippe.chavrier@curie.fr, pedro.monteiro@curie.fr, eformstecher@hybrigenics.com 2013 Only protein-protein interactions imex curation Coordinated function of WASH and exocyst complex underlies the mechanism of invadopodial matrix degradation by tumor cells Coordinated function of WASH and exocyst complex underlies the mechanism of invadopodial matrix degradation by tumor cells 2013 imex curation Monteiro P, Chavrier P, Formstecher E Affinomics - Interactions curated for the Affinomics consortium. philippe.chavrier@curie.fr, pedro.monteiro@curie.fr, eformstecher@hybrigenics.com 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2h fragment pooling two hybrid fragment pooling approach 2h fragment pooling nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Monteiro P, Chavrier P, Formstecher E Affinomics - Interactions curated for the Affinomics consortium. Confidence values - A,B and C correspond to interactions found in two reciprocal and independent screens or in a single screen with many overlapping prey fragments. D corresponds to data defined by single bait-singleton fragment interaction-this catogory would include false positives and low representation of the fragments in library. C corresponds to nonspecifically found prey fragments.E corresponds to interactions that may be classified as sticky. no idea where the Confidence limits came from as I cannot see anything on Proc6 regarding this. philippe.chavrier@curie.fr, pedro.monteiro@curie.fr, eformstecher@hybrigenics.com Accepted 2012-JUN-20 by ORCHARD Human placenta library 2013 Only protein-protein interactions imex curation Coordinated function of WASH and exocyst complex underlies the mechanism of invadopodial matrix degradation by tumor cells Coordinated function of WASH and exocyst complex underlies the mechanism of invadopodial matrix degradation by tumor cells 2013 imex curation Monteiro P, Chavrier P, Formstecher E Affinomics - Interactions curated for the Affinomics consortium. philippe.chavrier@curie.fr, pedro.monteiro@curie.fr, eformstecher@hybrigenics.com 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Monteiro P, Chavrier P, Formstecher E Affinomics - Interactions curated for the Affinomics consortium. philippe.chavrier@curie.fr, pedro.monteiro@curie.fr, eformstecher@hybrigenics.com Accepted 2012-JUN-20 by ORCHARD 2013 Only protein-protein interactions imex curation Coordinated function of WASH and exocyst complex underlies the mechanism of invadopodial matrix degradation by tumor cells Coordinated function of WASH and exocyst complex underlies the mechanism of invadopodial matrix degradation by tumor cells 2013 imex curation Monteiro P, Chavrier P, Formstecher E Affinomics - Interactions curated for the Affinomics consortium. philippe.chavrier@curie.fr, pedro.monteiro@curie.fr, eformstecher@hybrigenics.com 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Monteiro P, Chavrier P, Formstecher E Affinomics - Interactions curated for the Affinomics consortium. philippe.chavrier@curie.fr, pedro.monteiro@curie.fr, eformstecher@hybrigenics.com Accepted 2012-JUN-20 by ORCHARD 2013 Only protein-protein interactions imex curation Coordinated function of WASH and exocyst complex underlies the mechanism of invadopodial matrix degradation by tumor cells Coordinated function of WASH and exocyst complex underlies the mechanism of invadopodial matrix degradation by tumor cells 2013 imex curation Monteiro P, Chavrier P, Formstecher E Affinomics - Interactions curated for the Affinomics consortium. philippe.chavrier@curie.fr, pedro.monteiro@curie.fr, eformstecher@hybrigenics.com 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions human-bt_549 Homo sapiens BT_549 Breast ductal carcinoma cell line bt_549 BT 549 Human Breast ductal carcinoma cell line fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Monteiro P, Chavrier P, Formstecher E Affinomics - Interactions curated for the Affinomics consortium. philippe.chavrier@curie.fr, pedro.monteiro@curie.fr, eformstecher@hybrigenics.com Accepted 2012-JUN-20 by ORCHARD 2013 Only protein-protein interactions imex curation Coordinated function of WASH and exocyst complex underlies the mechanism of invadopodial matrix degradation by tumor cells Coordinated function of WASH and exocyst complex underlies the mechanism of invadopodial matrix degradation by tumor cells 2013 imex curation Monteiro P, Chavrier P, Formstecher E Affinomics - Interactions curated for the Affinomics consortium. philippe.chavrier@curie.fr, pedro.monteiro@curie.fr, eformstecher@hybrigenics.com 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Monteiro P, Chavrier P, Formstecher E Affinomics - Interactions curated for the Affinomics consortium. philippe.chavrier@curie.fr, pedro.monteiro@curie.fr, eformstecher@hybrigenics.com Accepted 2012-JUN-20 by ORCHARD 2013 Only protein-protein interactions imex curation Coordinated function of WASH and exocyst complex underlies the mechanism of invadopodial matrix degradation by tumor cells Coordinated function of WASH and exocyst complex underlies the mechanism of invadopodial matrix degradation by tumor cells 2013 imex curation Monteiro P, Chavrier P, Formstecher E Affinomics - Interactions curated for the Affinomics consortium. philippe.chavrier@curie.fr, pedro.monteiro@curie.fr, eformstecher@hybrigenics.com 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Monteiro P, Chavrier P, Formstecher E Affinomics - Interactions curated for the Affinomics consortium. philippe.chavrier@curie.fr, pedro.monteiro@curie.fr, eformstecher@hybrigenics.com 2013 Only protein-protein interactions imex curation exoc8_human Exocyst complex component 8 Exocyst complex 84 kDa subunit EXOC8 protein protein human Homo sapiens Human MAMAMSDSGASRLRRQLESGGFEARLYVKQLSQQSDGDRDLQEHRQRIQALAEETAQNLKRNVYQNYRQFIETAREISYLESEMYQLSHLLTEQKSSLESIPLTLLPAAAAAGAAAASGGEEGVGGAGGRDHLRGQAGFFSTPGGASRDGSGPGEEGKQRTLTTLLEKVEGCRHLLETPGQYLVYNGDLVEYDADHMAQLQRVHGFLMNDCLLVATWLPQRRGMYRYNALYSLDGLAVVNVKDNPPMKDMFKLLMFPESRIFQAENAKIKREWLEVLEDTKRALSEKRRREQEEAAAPRGPPQVTSKATNPFEDDEEEEPAVPEVEEEKVDLSMEWIQELPEDLDVCIAQRDFEGAVDLLDKLNHYLEDKPSPPPVKELRAKVEERVRQLTEVLVFELSPDRSLRGGPKATRRAVSQLIRLGQCTKACELFLRNRAAAVHTAIRQLRIEGATLLYIHKLCHVFFTSLLETAREFEIDFAGTDSGCYSAFVVWARSAMGMFVDAFSKQVFDSKESLSTAAECVKVAKEHCQQLGDIGLDLTFIIHALLVKDIQGALHSYKEIIIEATKHRNSEEMWRRMNLMTPEALGKLKEEMKSCGVSNFEQYTGDDCWVNLSYTVVAFTKQTMGFLEEALKLYFPELHMVLLESLVEIILVAVQHVDYSLRCEQDPEKKAFIRQNASFLYETVLPVVEKRFEEGVGKPAKQLQDLRNASRLIRVNPESTTSVV F4E5E344EF24FDCA src8_human Src substrate cortactin CTTN EMS1 Amplaxin Oncogene EMS1 protein protein human Homo sapiens Human MWKASAGHAVSIAQDDAGADDWETDPDFVNDVSEKEQRWGAKTVQGSGHQEHINIHKLRENVFQEHQTLKEKELETGPKASHGYGGKFGVEQDRMDKSAVGHEYQSKLSKHCSQVDSVRGFGGKFGVQMDRVDQSAVGFEYQGKTEKHASQKDYSSGFGGKYGVQADRVDKSAVGFDYQGKTEKHESQRDYSKGFGGKYGIDKDKVDKSAVGFEYQGKTEKHESQKDYVKGFGGKFGVQTDRQDKCALGWDHQEKLQLHESQKDYKTGFGGKFGVQSERQDSAAVGFDYKEKLAKHESQQDYSKGFGGKYGVQKDRMDKNASTFEDVTQVSSAYQKTVPVEAVTSKTSNIRANFENLAKEKEQEDRRKAEAERAQRMAKERQEQEEARRKLEEQARAKTQTPPVSPAPQPTEERLPSSPVYEDAASFKAELSYRGPVSGTEPEPVYSMEAADYREASSQQGLAYATEAVYESAEAPGHYPAEDSTYDEYENDLGITAVALYDYQAAGDDEISFDPDDIITNIEMIDDGWWRGVCKGRYGLFPANYVELRQ DIP protein Q14247 original sequence version: 148 7799326C2B4383BB src8_rat Src substrate cortactin Cttn protein protein rat Rattus norvegicus (Rat) Rat MWKASAGHAVSITQDDGGADDWETDPDFVNDVSEKEQRWGAKTVQGSGHQEHINIHKLRENVFQEHQTLKEKELETGPKASHGYGGKFGVEQDRMDKSAVGHEYQSKLSKHCSQVDSVRGFGGKFGVQMDRVDQSAVGFEYQGKTEKHASQKDYSSGFGGKYGVQADRVDKSAVGFDYQGKTEKHESQKDYSKGFGGKYGIDKDKVDKSAVGFEYQGKTEKHESQKDYVKGFGGKFGVQTDRQDKCALGWDHQEKLQLHESQKDYAKGFGGKYGVQKDRMDKNASTFEEVVQVPSAYQKTVPIEAVTSKTSNIRANFENLAKEREQEDRRKAEAERAQRMAQERQEQEEARRKLEEQARAKKQTPPASPSPQPAEDRPPSSPIYEDAAPLKAEPSYGSSEPEPEYSTEAAGLPEASNQQGLAYTSEPVYETTEVPGHYQAEDDTYDGYESDLGITAIALYDYQAAGDDEISFDPDDVITNIEMIDDGWWRGVCKGRYGLFPANYVELRQ 059CDB52681E2592 wash1_mouse WASH complex subunit 1 Washc1 Orf19 WAS protein family homolog 1 Wash Wash1 protein protein mouse Mus musculus Mouse MTPVKTQCSLAGQLYAVPLIQPDLRREEAIQQVADALQYLQNISGDIFSRISRRVELSRRQLQAISERVSLAQAKIEKIKGSKKAIKVFSSAKYPAPEHLQEYGSIFTGALDPGLQRRPRYRIQSKHRPLDERALQEKLKYFPVCVNTKSEPEDEAEEGLGGLPSNISSISSLLLFNTTENLYKKYVFLDPLAGAVTKTHTMLGTEEEKLFDAPLSISKREQLERQAPENYFYVPDLGQVPEIDVPSYLPDLPGVADDLMYSADLGPGIAPSAPGAIPELPAFHTEVAEPLQPELENEVLLAAPPPPPPPPPPPPPAPTALVSTPQPPMFPDMATAAGQVAREEDSSSSMAHTASVQGAPKEVVDPSSGRATLLESIRQAGGIGKAKLRSVKERKLEKKKQKEQEQVRATSQGGDLMSDLFNKLVMRRKGISGKGPSTGTSEGPGGAFSRMSDSIPPLPPPQQPAGDEDEEDWES 4198C8AEE499D3C3 exoc1_human Exocyst complex component 1 Exocyst complex component Sec3 EXOC1 SEC3 SEC3L1 BM-012 protein protein human Homo sapiens Human MTAIKHALQRDIFTPNDERLLSIVNVCKAGKKKKNCFLCATVTTERPVQVKVVKVKKSDKGDFYKRQIAWALRDLAVVDAKDAIKENPEFDLHFEKIYKWVASSTAEKNAFISCIWKLNQRYLRKKIDFVNVSSQLLEESVPSGENQSVTGGDEEVVDEYQELNAREEQDIEIMMEGCEYAISNAEAFAEKLSRELQVLDGANIQSIMASEKQVNILMKLLDEALKEVDQIELKLSSYEEMLQSVKEQMDQISESNHLIHLSNTNNVKLLSEIEFLVNHMDLAKGHIKALQEGDLASSRGIEACTNAADALLQCMNVALRPGHDLLLAVKQQQQRFSDLRELFARRLASHLNNVFVQQGHDQSSTLAQHSVELTLPNHHPFHRDLLRYAKLMEWLKSTDYGKYEGLTKNYMDYLSRLYEREIKDFFEVAKIKMTGTTKESKKFATLPRKESAVKQETESLHGSSGKLTGSTSSLNKLSVQSSGNRRSQSSSLLDMGNMSASDLDVADRTKFDKIFEQVLSELEPLCLAEQDFISKFFKLQQHQSMPGTMAEAEDLDGGTLSRQHNCGTPLPVSSEKDMIRQMMIKIFRCIEPELNNLIALGDKIDSFNSLYMLVKMSHHVWTAQNVDPASFLSTTLGNVLVTVKRNFDKCISNQIRQMEEVKISKKSKVGILPFVAEFEEFAGLAESIFKNAERRGDLDKAYTKLIRGVFVNVEKVANESQKTPRDVVMMENFHHIFATLSRLKISCLEAEKKEAKQKYTDHLQSYVIYSLGQPLEKLNHFFEGVEARVAQGIREEEVSYQLAFNKQELRKVIKEYPGKEVKKGLDNLYKKVDKHLCEEENLLQVVWHSMQDEFIRQYKHFEGLIARCYPGSGVTMEFTIQDILDYCSSIAQSH DIP protein Q9NV70 original sequence version: 75 F63C88E3A7F611BD wash1_human WASH complex subunit 1 WASHC1 CXYorf1-like protein on chromosome 9 FAM39E Protein FAM39E WAS protein family homolog 1 WASH1 protein protein human Homo sapiens Human MTPVRMQHSLAGQTYAVPFIQPDLRREEAVQQMADALQYLQKVSGDIFSRISQQVEQSRSQVQAIGEKVSLAQAKIEKIKGSKKAIKVFSSAKYPAPGRLQEYGSIFTGAQDPGLQRRPRHRIQSKHRPLDERALQEKLKDFPVCVSTKPEPEDDAEEGLGGLPSNISSVSSLLLFNTTENLYKKYVFLDPLAGAVTKTHVMLGAETEEKLFDAPLSISKREQLEQQVPENYFYVPDLGQVPEIHVPSYLPDLPGIANDLMYSADLGPGIAPSAPGTIPELPTFHTEVAEPLKVDLQDGVLTPPPPPPPPPPAPEVLASAPPLPPSTAAPVGQGARQDDSSSSASPSVQGAPREVVDPSGGWATLLESIRQAGGIGKAKLRSMKERKLEKQQQKEQEQVRATSQGGHLMSDLFNKLVMRRKGISGKGPGAGEGPGGAFVRVSDSIPPLPPPQQPQAEEDEDDWES DBD5DEAFB7019B3E exoc4_human Exocyst complex component 4 EXOC4 KIAA1699 SEC8 SEC8L1 Exocyst complex component Sec8 protein protein human Homo sapiens Human MAAEAAGGKYRSTVSKSKDPSGLLISVIRTLSTSDDVEDRENEKGRLEEAYEKCDRDLDELIVQHYTELTTAIRTYQSITERITNSRNKIKQVKENLLSCKMLLHCKRDELRKLWIEGIEHKHVLNLLDEIENIKQVPQKLEQCMASKHYLSATDMLVSAVESLEGPLLQVEGLSDLRLELHSKKMNLHLVLIDELHRHLYIKSTSRVVQRNKEKGKISSLVKDASVPLIDVTNLPTPRKFLDTSHYSTAGSSSVREINLQDIKEDLELDPEENSTLFMGILIKGLAKLKKIPETVKAIIERLEQELKQIVKRSTTQVADSGYQRGENVTVENQPRLLLELLELLFDKFNAVAAAHSVVLGYLQDTVVTPLTQQEDIKLYDMADVWVKIQDVLQMLLTEYLDMKNTRTASEPSAQLSYASTGREFAAFFAKKKPQRPKNSLFKFESSSHAISMSAYLREQRRELYSRSGELQGGPDDNLIEGGGTKFVCKPGARNITVIFHPLLRFIQEIEHALGLGPAKQCPLREFLTVYIKNIFLNQVLAEINKEIEGVTKTSDPLKILANADTMKVLGVQRPLLQSTIIVEKTVQDLLNLMHDLSAYSDQFLNMVCVKLQEYKDTCTAAYRGIVQSEEKLVISASWAKDDDISRLLKSLPNWMNMAQPKQLRPKREEEEDFIRAAFGKESEVLIGNLGDKLIPPQDILRDVSDLKALANMHESLEWLASRTKSAFSNLSTSQMLSPAQDSHTNTDLPPVSEQIMQTLSELAKSFQDMADRCLLVLHLEVRVHCFHYLIPLAKEGNYAIVANVESMDYDPLVVKLNKDISAIEEAMSASLQQHKFQYIFEGLGHLISCILINGAQYFRRISESGIKKMCRNIFVLQQNLTNITMSREADLDFARQYYEMLYNTADELLNLVVDQGVKYTELEYIHALTLLHRSQTGVGELTTQNTRLQRLKEIICEQAAIKQATKDKKITTV DIP protein Q96A65 original sequence version: 76 DIP protein Q96A65 original sequence version: 88 DIP protein Q96A65 original sequence version: 135 122CD929C2635786 exoc1-wash1 2 12 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association author score author-based confidence author score A no figure exoc8-wash1 2 8 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association author score author-based confidence author score B no figure wash1-exoc4 3 14 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait physical association physical association cttn-wash1 6 9 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component colocalization colocalization 1A/B, 2 D/F Silencing of WASH led to a large reduction of cortactin accumulation on endosomal compartments, whereas silencing of Exo84 was accompanied by a limited but significant increase of the size of WASH patches on MT1-MMP–positive endosomes and a marked lengthening of comet-shaped cortactin domains associated with WASH patches exoc8-wash1-1 6 8 unspecified role unspecified role neutral component neutral component 11 tag fluorescence tag visualisation by fluorescence tag fluorescence immunocytochemistry immunocytochemistry unspecified role unspecified role neutral component neutral component n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization 1C/D cttn-wash1-1 6 9 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization Supp 1B, Supp 3B exoc8-wash1-2 1 11 unspecified role unspecified role neutral component neutral component n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 10 unspecified role unspecified role neutral component neutral component c_terminus rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization Video 1, (video 7), figure 7D. cttn-wash1-2 5 13 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component colocalization colocalization Supp 1E exoc8-wash1-3 4 13 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 1F/G exoc8-wash1-4 4 9 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 1F/Gi wash1-exoc8-1 7 13 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey c_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association wash1-exoc1-1 7 13 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey c_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association