IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein. Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein. J. Virol. (0022-538X) 2014 imex curation Teo C.S., Chu J.J. Virus - Publications including interactions involving viral proteins Affinomics - Interactions curated for the Affinomics consortium. Viral strain is given as:-DENV-2 NGC strain (NCBI accession no. NC_001474.2). This corresponds to only one Uniprot Genome polyprotein = P14340. miccjh@nus.edu.sg 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Teo C.S., Chu J.J. Virus - Publications including interactions involving viral proteins Viral strain is given as:-DENV-2 NGC strain (NCBI accession no. NC_001474.2). This corresponds to only one Uniprot Genome polyprotein = P14340. miccjh@nus.edu.sg Accepted 2014-OCT-20 by ORCHARD J. Virol. (0022-538X) 2014 Only protein-protein interactions imex curation Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein. Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein. J. Virol. (0022-538X) 2014 imex curation Teo C.S., Chu J.J. Virus - Publications including interactions involving viral proteins Affinomics - Interactions curated for the Affinomics consortium. Viral strain is given as:-DENV-2 NGC strain (NCBI accession no. NC_001474.2). This corresponds to only one Uniprot Genome polyprotein = P14340. miccjh@nus.edu.sg 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Teo C.S., Chu J.J. Virus - Publications including interactions involving viral proteins Viral strain is given as:-DENV-2 NGC strain (NCBI accession no. NC_001474.2). This corresponds to only one Uniprot Genome polyprotein = P14340. miccjh@nus.edu.sg Accepted 2014-OCT-20 by ORCHARD J. Virol. (0022-538X) 2014 Only protein-protein interactions imex curation Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein. Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein. J. Virol. (0022-538X) 2014 imex curation Teo C.S., Chu J.J. Virus - Publications including interactions involving viral proteins Affinomics - Interactions curated for the Affinomics consortium. Viral strain is given as:-DENV-2 NGC strain (NCBI accession no. NC_001474.2). This corresponds to only one Uniprot Genome polyprotein = P14340. miccjh@nus.edu.sg 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Teo C.S., Chu J.J. Virus - Publications including interactions involving viral proteins Virus - Publications including interactions involving viral proteins Viral strain is given as:-DENV-2 NGC strain (NCBI accession no. NC_001474.2). This corresponds to only one Uniprot Genome polyprotein = P14340. miccjh@nus.edu.sg Accepted 2014-OCT-20 by ORCHARD J. Virol. (0022-538X) 2014 Only protein-protein interactions imex curation Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein. Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein. J. Virol. (0022-538X) 2014 imex curation Teo C.S., Chu J.J. Virus - Publications including interactions involving viral proteins Affinomics - Interactions curated for the Affinomics consortium. Viral strain is given as:-DENV-2 NGC strain (NCBI accession no. NC_001474.2). This corresponds to only one Uniprot Genome polyprotein = P14340. miccjh@nus.edu.sg 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Teo C.S., Chu J.J. Virus - Publications including interactions involving viral proteins Virus - Publications including interactions involving viral proteins Viral strain is given as:-DENV-2 NGC strain (NCBI accession no. NC_001474.2). This corresponds to only one Uniprot Genome polyprotein = P14340. miccjh@nus.edu.sg Accepted 2014-OCT-20 by ORCHARD J. Virol. (0022-538X) 2014 Only protein-protein interactions imex curation Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein. Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein. J. Virol. (0022-538X) 2014 imex curation Teo C.S., Chu J.J. Virus - Publications including interactions involving viral proteins Affinomics - Interactions curated for the Affinomics consortium. Viral strain is given as:-DENV-2 NGC strain (NCBI accession no. NC_001474.2). This corresponds to only one Uniprot Genome polyprotein = P14340. miccjh@nus.edu.sg 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Teo C.S., Chu J.J. Virus - Publications including interactions involving viral proteins Virus - Publications including interactions involving viral proteins Viral strain is given as:-DENV-2 NGC strain (NCBI accession no. NC_001474.2). This corresponds to only one Uniprot Genome polyprotein = P14340. miccjh@nus.edu.sg Accepted 2014-OCT-20 by ORCHARD J. Virol. (0022-538X) 2014 Only protein-protein interactions imex curation Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein. Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein. J. Virol. (0022-538X) 2014 imex curation Teo C.S., Chu J.J. Virus - Publications including interactions involving viral proteins Affinomics - Interactions curated for the Affinomics consortium. Viral strain is given as:-DENV-2 NGC strain (NCBI accession no. NC_001474.2). This corresponds to only one Uniprot Genome polyprotein = P14340. miccjh@nus.edu.sg 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma tap tandem affinity purification TAP TAP tap sequence tag sequence tag identification sequence tag Teo C.S., Chu J.J. Virus - Publications including interactions involving viral proteins Virus - Publications including interactions involving viral proteins The two affinity tags used are: a streptavidin binding peptide (SBP) and a calmodulin binding peptide (CBP). Viral strain is given as:-DENV-2 NGC strain (NCBI accession no. NC_001474.2). This corresponds to only one Uniprot Genome polyprotein = P14340. miccjh@nus.edu.sg Accepted 2014-OCT-20 by ORCHARD J. Virol. (0022-538X) 2014 Only protein-protein interactions imex curation Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein. Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein. J. Virol. (0022-538X) 2014 imex curation Teo C.S., Chu J.J. Virus - Publications including interactions involving viral proteins Affinomics - Interactions curated for the Affinomics consortium. Viral strain is given as:-DENV-2 NGC strain (NCBI accession no. NC_001474.2). This corresponds to only one Uniprot Genome polyprotein = P14340. miccjh@nus.edu.sg 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma scanning electron microscopy scanning electron microscopy sem sem antibody detection identification by antibody antibody detection Teo C.S., Chu J.J. Virus - Publications including interactions involving viral proteins Virus - Publications including interactions involving viral proteins not sure about adding this interaction:Manual states:- "Gold immunostaining data (using Electron density methods/ electron microscopy) is acceptable as co-localization data when there is a clear distinction between the labelled particles, usually on the basis of size." I am not sure if there is a "clear distinction" here, but have entered it. Not sure what to put for participant det. - all existing entries are "predetermined" or tag - vis - which is not appropriate here. Viral strain is given as:-DENV-2 NGC strain (NCBI accession no. NC_001474.2). This corresponds to only one Uniprot Genome polyprotein = P14340. miccjh@nus.edu.sg Accepted 2014-OCT-20 by ORCHARD J. Virol. (0022-538X) 2014 Only protein-protein interactions imex curation p14340-pro_0000037966 Non-structural protein 4A protein protein den2n Dengue virus type 2 (strain Thailand/NGS-C/1944) (DENV-2) SLTLNLITEMGRLPTFMTQKARDALDNLAVLHTAEAGGRAYNHALSELPETLETLLLLTLLATVTGGIFLFLMSGRGIGKMTLGMCCIITASILLWYAQIQPHWIAASIILEFFLIVLLIPEPEKQR 2094 2220 52E0DB8BAD22E654 actb_human Actin, cytoplasmic 1 ACTB Beta-actin protein protein human Homo sapiens Human MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF DIP protein P60709 original sequence version: null DIP protein P60709 original sequence version: 63 DIP protein P60709 original sequence version: 128 DIP protein P60709 original sequence version: 129 DIP protein P60709 original sequence version: 58 DIP protein P60709 original sequence version: 66 DIP protein P60709 original sequence version: 88 DIP protein P60709 original sequence version: 89 DIP protein P60709 original sequence version: 86 DIP protein P60709 original sequence version: 138 DIP protein P60709 original sequence version: 112 6AFD05CA94E360E2 ef1a1_human Elongation factor 1-alpha 1 EEF1A1 EEF1A EF1A LENG7 Eukaryotic elongation factor 1 A-1 Elongation factor Tu Leukocyte receptor cluster member 7 protein protein human Homo sapiens Human MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKKAAGAGKVTKSAQKAQKAK DIP protein P68104 original sequence version: null DIP protein P68104 original sequence version: 134 D465615545AF686A tbb5_human Tubulin beta chain TUBB Tubulin beta-5 chain TUBB5 OK/SW-cl.56 protein protein human Homo sapiens Human MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEEDFGEEAEEEA DIP protein P07437 original sequence version: 170 1E6CD0A36773A103 g6pd_human Glucose-6-phosphate 1-dehydrogenase G6PD protein protein human Homo sapiens Human MAEQVALSRTQVCGILREELFQGDAFHQSDTHIFIIMGASGDLAKKKIYPTIWWLFRDGLLPENTFIVGYARSRLTVADIRKQSEPFFKATPEEKLKLEDFFARNSYVAGQYDDAASYQRLNSHMNALHLGSQANRLFYLALPPTVYEAVTKNIHESCMSQIGWNRIIVEKPFGRDLQSSDRLSNHISSLFREDQIYRIDHYLGKEMVQNLMVLRFANRIFGPIWNRDNIACVILTFKEPFGTEGRGGYFDEFGIIRDVMQNHLLQMLCLVAMEKPASTNSDDVRDEKVKVLKCISEVQANNVVLGQYVGNPDGEGEATKGYLDDPTVPRGSTTATFAAVVLYVENERWDGVPFILRCGKALNERKAEVRLQFHDVAGDIFHQQCKRNELVIRVQPNEAVYTKMMTKKPGMFFNPEESELDLTYGNRYKNVKLPDAYERLILDVFCGSQMHFVRSDELREAWRIFTPLLHQIELEKPKPIPYIYGSRGPTEADELMKRVGFQYEGTYKWVNPHKL F2B775340640A96F gdib_human Rab GDP dissociation inhibitor beta Guanosine diphosphate dissociation inhibitor 2 GDI2 RABGDIB protein protein human Homo sapiens Human MNEEYDVIVLGTGLTECILSGIMSVNGKKVLHMDRNPYYGGESASITPLEDLYKRFKIPGSPPESMGRGRDWNVDLIPKFLMANGQLVKMLLYTEVTRYLDFKVTEGSFVYKGGKIYKVPSTEAEALASSLMGLFEKRRFRKFLVYVANFDEKDPRTFEGIDPKKTTMRDVYKKFDLGQDVIDFTGHALALYRTDDYLDQPCYETINRIKLYSESLARYGKSPYLYPLYGLGELPQGFARLSAIYGGTYMLNKPIEEIIVQNGKVIGVKSEGEIARCKQLICDPSYVKDRVEKVGQVIRVICILSHPIKNTNDANSCQIIIPQNQVNRKSDIYVCMISFAHNVAAQGKYIAIVSTTVETKEPEKEIRPALELLEPIEQKFVSISDLLVPKDLGTESQIFISRTYDATTHFETTCDDIKNIYKRMTGSEFDFEEMKRKKNDIYGED DIP protein P50395 original sequence version: 92 DIP protein P50395 original sequence version: 137 CE186A2E3A47FCC9 kpym_human Pyruvate kinase PKM PKM Cytosolic thyroid hormone-binding protein OIP3 Opa-interacting protein 3 PK2 PK3 PKM2 Pyruvate kinase 2/3 Pyruvate kinase muscle isozyme Thyroid hormone-binding protein 1 Tumor M2-PK p58 protein protein human Homo sapiens Human MSKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP DIP protein P14618 original sequence version: 162 AA94D7818ED6BBAD vime_human Vimentin VIM protein protein human Homo sapiens Human MSTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHEEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESTEYRRQVQSLTCEVDALKGTNESLERQMREMEENFAVEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPNFSSLNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE DIP protein P08670 original sequence version: 195 DIP protein P08670 original sequence version: 209 DIP protein P08670 original sequence version: 192 DIP protein P08670 original sequence version: 175 BAB54026665B015A enoa_human Alpha-enolase ENO1 ENO1L1 MBPB1 MPB1 2-phospho-D-glycerate hydro-lyase Non-neural enolase Enolase 1 Phosphopyruvate hydratase C-myc promoter-binding protein MBP-1 MPB-1 Plasminogen-binding protein protein protein human Homo sapiens Human MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK DIP protein P06733 original sequence version: null A0ED663FCC15ADA5 p14340_pro-actb 6 16 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait region tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Table 1, Figure 1 p14340_pro-actb-1 4 8 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey physical association physical association Figure 1B vim-p14340_pro 4 8 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait physical association physical association Figure 1B p14340_pro-vim-1 5 15 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization Figure 2,3,9, 10. Colocalization of vimentin with NS4A was lost upon knockdown of CaMKII-gamma. KN93 -a specific CaMK II inhibitor p14340_pro-vim 3 15 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction Figure 3D vim-p14340_pro-3 7 15 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization Figure 4 p14340_pro-vim-2 5 8 tag visualisation tag visualisation immunocytochemistry immunocytochemistry unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 15 unspecified role unspecified role neutral component neutral component colocalization colocalization Figure 5 p14340_pro-vim-3 3 8 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 15 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction Figure 6 p14340_pro-vim-5 1 15 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 6D vim-p14340_pro-4 2 15 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 6D