HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html HCV NS3 protease enhances liver fibrosis via binding to and activating TGF-β type I receptor. HCV NS3 protease enhances liver fibrosis via binding to and activating TGF-β type I receptor. Sci Rep 2013 imex curation Sakata K., Hara M., Terada T., Watanabe N., Takaya D., Yaguchi S., Matsumoto T., Matsuura T., Shirouzu M., Yokoyama S., Yamaguchi T., Miyazawa K., Aizaki H., Suzuki T., Wakita T., Imoto M., Kojima S. Affinomics - Interactions curated for the Affinomics consortium. Virus - Publications including interactions involving viral proteins skojima@riken.jp 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Sakata K., Hara M., Terada T., Watanabe N., Takaya D., Yaguchi S., Matsumoto T., Matsuura T., Shirouzu M., Yokoyama S., Yamaguchi T., Miyazawa K., Aizaki H., Suzuki T., Wakita T., Imoto M., Kojima S. Affinomics - Interactions curated for the Affinomics consortium. skojima@riken. jp skojima@riken.jp Accepted 2014-JAN-16 by ORCHARD Sci Rep 2013 imex curation HCV NS3 protease enhances liver fibrosis via binding to and activating TGF-β type I receptor. HCV NS3 protease enhances liver fibrosis via binding to and activating TGF-β type I receptor. Sci Rep 2013 imex curation Sakata K., Hara M., Terada T., Watanabe N., Takaya D., Yaguchi S., Matsumoto T., Matsuura T., Shirouzu M., Yokoyama S., Yamaguchi T., Miyazawa K., Aizaki H., Suzuki T., Wakita T., Imoto M., Kojima S. Affinomics - Interactions curated for the Affinomics consortium. Virus - Publications including interactions involving viral proteins skojima@riken.jp 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Sakata K., Hara M., Terada T., Watanabe N., Takaya D., Yaguchi S., Matsumoto T., Matsuura T., Shirouzu M., Yokoyama S., Yamaguchi T., Miyazawa K., Aizaki H., Suzuki T., Wakita T., Imoto M., Kojima S. Affinomics - Interactions curated for the Affinomics consortium. skojima@riken. jp skojima@riken.jp Accepted 2014-JAN-16 by ORCHARD Sci Rep 2013 imex curation HCV NS3 protease enhances liver fibrosis via binding to and activating TGF-β type I receptor. HCV NS3 protease enhances liver fibrosis via binding to and activating TGF-β type I receptor. Sci Rep 2013 imex curation Sakata K., Hara M., Terada T., Watanabe N., Takaya D., Yaguchi S., Matsumoto T., Matsuura T., Shirouzu M., Yokoyama S., Yamaguchi T., Miyazawa K., Aizaki H., Suzuki T., Wakita T., Imoto M., Kojima S. Affinomics - Interactions curated for the Affinomics consortium. Virus - Publications including interactions involving viral proteins skojima@riken.jp 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh-7.5 Human Huh-7.5 cells huh75 Huh-7.5 cells confocal microscopy confocal microscopy immunostaining immunostaining Sakata K., Hara M., Terada T., Watanabe N., Takaya D., Yaguchi S., Matsumoto T., Matsuura T., Shirouzu M., Yokoyama S., Yamaguchi T., Miyazawa K., Aizaki H., Suzuki T., Wakita T., Imoto M., Kojima S. Affinomics - Interactions curated for the Affinomics consortium. skojima@riken. jp skojima@riken.jp Accepted 2014-JAN-16 by ORCHARD Sci Rep 2013 imex curation HCV NS3 protease enhances liver fibrosis via binding to and activating TGF-β type I receptor. HCV NS3 protease enhances liver fibrosis via binding to and activating TGF-β type I receptor. Sci Rep 2013 imex curation Sakata K., Hara M., Terada T., Watanabe N., Takaya D., Yaguchi S., Matsumoto T., Matsuura T., Shirouzu M., Yokoyama S., Yamaguchi T., Miyazawa K., Aizaki H., Suzuki T., Wakita T., Imoto M., Kojima S. Affinomics - Interactions curated for the Affinomics consortium. Virus - Publications including interactions involving viral proteins skojima@riken.jp 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh-7.5 Human Huh-7.5 cells huh75 Huh-7.5 cells pla proximity ligation assay pELISA pELISA PLA immunostaining immunostaining Sakata K., Hara M., Terada T., Watanabe N., Takaya D., Yaguchi S., Matsumoto T., Matsuura T., Shirouzu M., Yokoyama S., Yamaguchi T., Miyazawa K., Aizaki H., Suzuki T., Wakita T., Imoto M., Kojima S. Affinomics - Interactions curated for the Affinomics consortium. skojima@riken. jp skojima@riken.jp Accepted 2014-JAN-16 by ORCHARD Sci Rep 2013 imex curation tgfr2_human TGF-beta receptor type-2 TGFBR2 Transforming growth factor-beta receptor type II TGF-beta type II receptor protein protein human Homo sapiens Human MGRGLLRGLWPLHIVLWTRIASTIPPHVQKSVNNDMIVTDNNGAVKFPQLCKFCDVRFSTCDNQKSCMSNCSITSICEKPQEVCVAVWRKNDENITLETVCHDPKLPYHDFILEDAASPKCIMKEKKKPGETFFMCSCSSDECNDNIIFSEEYNTSNPDLLLVIFQVTGISLLPPLGVAISVIIIFYCYRVNRQQKLSSTWETGKTRKLMEFSEHCAIILEDDRSDISSTCANNINHNTELLPIELDTLVGKGRFAEVYKAKLKQNTSEQFETVAVKIFPYEEYASWKTEKDIFSDINLKHENILQFLTAEERKTELGKQYWLITAFHAKGNLQEYLTRHVISWEDLRKLGSSLARGIAHLHSDHTPCGRPKMPIVHRDLKSSNILVKNDLTCCLCDFGLSLRLDPTLSVDDLANSGQVGTARYMAPEVLESRMNLENVESFKQTDVYSMALVLWEMTSRCNAVGEVKDYEPPFGSKVREHPCVESMKDNVLRDRGRPEIPSFWLNHQGIQMVCETLTECWDHDPEARLTAQCVAERFSELEHLDRLSGRSCSEEKIPEDGSLNTTK DIP protein P37173 original sequence version: 163 C541DA751FFBDBEB tgfr1_human TGF-beta receptor type-1 TGFBR1 Transforming growth factor-beta receptor type I TGF-beta type I receptor Serine/threonine-protein kinase receptor R4 Activin receptor-like kinase 5 ALK5 SKR4 Activin A receptor type II-like protein kinase of 53kD protein protein human Homo sapiens Human MEAAVAAPRPRLLLLVLAAAAAAAAALLPGATALQCFCHLCTKDNFTCVTDGLCFVSVTETTDKVIHNSMCIAEIDLIPRDRPFVCAPSSKTGSVTTTYCCNQDHCNKIELPTTVKSSPGLGPVELAAVIAGPVCFVCISLMLMVYICHNRTVIHHRVPNEEDPSLDRPFISEGTTLKDLIYDMTTSGSGSGLPLLVQRTIARTIVLQESIGKGRFGEVWRGKWRGEEVAVKIFSSREERSWFREAEIYQTVMLRHENILGFIAADNKDNGTWTQLWLVSDYHEHGSLFDYLNRYTVTVEGMIKLALSTASGLAHLHMEIVGTQGKPAIAHRDLKSKNILVKKNGTCCIADLGLAVRHDSATDTIDIAPNHRVGTKRYMAPEVLDDSINMKHFESFKRADIYAMGLVFWEIARRCSIGGIHEDYQLPYYDLVPSDPSVEEMRKVVCEQKLRPNIPNRWQSCEALRVMAKIMRECWYANGAARLTALRIKKTLSQLSQQEGIKM DIP protein P36897 original sequence version: 161 179F11404725DDCB q99ib8-pro_0000045599 Serine protease/helicase NS3 protein protein hcvjf Hepatitis C virus genotype 2a (isolate JFH-1) HCV APITAYAQQTRGLLGAIVVSMTGRDRTEQAGEVQILSTVSQSFLGTTISGVLWTVYHGAGNKTLAGLRGPVTQMYSSAEGDLVGWPSPPGTKSLEPCKCGAVDLYLVTRNADVIPARRRGDKRGALLSPRPISTLKGSSGGPVLCPRGHVVGLFRAAVCSRGVAKSIDFIPVETLDVVTRSPTFSDNSTPPAVPQTYQVGYLHAPTGSGKSTKVPVAYAAQGYKVLVLNPSVAATLGFGAYLSKAHGINPNIRTGVRTVMTGEAITYSTYGKFLADGGCASGAYDIIICDECHAVDATSILGIGTVLDQAETAGVRLTVLATATPPGSVTTPHPDIEEVGLGREGEIPFYGRAIPLSCIKGGRHLIFCHSKKKCDELAAALRGMGLNAVAYYRGLDVSIIPAQGDVVVVATDALMTGYTGDFDSVIDCNVAVTQAVDFSLDPTFTITTQTVPQDAVSRSQRRGRTGRGRQGTYRYVSTGERASGMFDSVVLCECYDAGAAWYDLTPAETTVRLRAYFNTPGLPVCQDHLEFWEAVFTGLTHIDAHFLSQTKQAGENFAYLVAYQATVCARAKAPPPSWDAMWKCLARLKPTLAGPTPLLYRLGPITNEVTLTHPGTKYIATCMQADLEVMT 1031 1661 B6CEB07928DC653D ns3-tbr1-2 3 6 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component colocalization colocalization Figure 3B ns3-tbr1-1 4 7 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction Figure 3C ns3-tbr1-3 2 7 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 3D and supp. data S5 ns3-tbr2-1 2 5 unspecified role unspecified role prey prey his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 3D and supp. data S5 ns3-tbr1-4 1 7 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction Supp. data S7A tgfbr1-ns3-competitor-1 1 7 competitor competitor competitor prey prey gly120-ser139 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. data S7A