IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress. Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress. Biochem. J. (0264-6021) 2013 imex curation Kern F., Stanika R.I., Sarg B., Offterdinger M., Hess D., Obermair G.J., Lindner H., Bandtlow C.E., Hengst L., Schweigreiter R. Affinomics - Interactions curated for the Affinomics consortium. ruediger.schweigreiter@i-med.ac.at 20-Oct-2014: Contacted by IntAct-Help. Only protein-protein interactions crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Kern F., Stanika R.I., Sarg B., Offterdinger M., Hess D., Obermair G.J., Lindner H., Bandtlow C.E., Hengst L., Schweigreiter R. Affinomics - Interactions curated for the Affinomics consortium. the text states they did PLA in Primary Hopp. Neurons - but I can find no image for this, so not entered. ruediger.schweigreiter@i-med.ac.at Accepted 2014-OCT-07 by ORCHARD Biochem. J. (0264-6021) 2013 Only protein-protein interactions imex curation Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress. Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress. Biochem. J. (0264-6021) 2013 imex curation Kern F., Stanika R.I., Sarg B., Offterdinger M., Hess D., Obermair G.J., Lindner H., Bandtlow C.E., Hengst L., Schweigreiter R. Affinomics - Interactions curated for the Affinomics consortium. ruediger.schweigreiter@i-med.ac.at 20-Oct-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-brain_hippocampal_neuron Mus musculus, brain, hippocampal neurons brain-hippocampus_neuron Hippocampal neuron pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Kern F., Stanika R.I., Sarg B., Offterdinger M., Hess D., Obermair G.J., Lindner H., Bandtlow C.E., Hengst L., Schweigreiter R. Affinomics - Interactions curated for the Affinomics consortium. ruediger.schweigreiter@i-med.ac.at Accepted 2014-OCT-07 by ORCHARD Biochem. J. (0264-6021) 2013 Only protein-protein interactions imex curation Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress. Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress. Biochem. J. (0264-6021) 2013 imex curation Kern F., Stanika R.I., Sarg B., Offterdinger M., Hess D., Obermair G.J., Lindner H., Bandtlow C.E., Hengst L., Schweigreiter R. Affinomics - Interactions curated for the Affinomics consortium. ruediger.schweigreiter@i-med.ac.at 20-Oct-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down sequence tag sequence tag identification sequence tag Kern F., Stanika R.I., Sarg B., Offterdinger M., Hess D., Obermair G.J., Lindner H., Bandtlow C.E., Hengst L., Schweigreiter R. Affinomics - Interactions curated for the Affinomics consortium. Column eluates were either briefly run via SDS/PAGE followed by LC-MS/MS, or were TCA-precipitated and then analysed by LC-MS/MS. The protein (genbank ID) EDL09793.1. has a different N-terminal sequence from any protein currently in UniProt. ruediger.schweigreiter@i-med.ac.at Accepted 2014-OCT-07 by ORCHARD Biochem. J. (0264-6021) 2013 Only protein-protein interactions imex curation Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress. Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress. Biochem. J. (0264-6021) 2013 imex curation Kern F., Stanika R.I., Sarg B., Offterdinger M., Hess D., Obermair G.J., Lindner H., Bandtlow C.E., Hengst L., Schweigreiter R. Affinomics - Interactions curated for the Affinomics consortium. ruediger.schweigreiter@i-med.ac.at 20-Oct-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-brain Mus musculus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kern F., Stanika R.I., Sarg B., Offterdinger M., Hess D., Obermair G.J., Lindner H., Bandtlow C.E., Hengst L., Schweigreiter R. Affinomics - Interactions curated for the Affinomics consortium. I have not added any isoform info for the mouse RTN4 - as isoform lengths differ a lot from Rat. ruediger.schweigreiter@i-med.ac.at Accepted 2014-OCT-07 by ORCHARD Biochem. J. (0264-6021) 2013 Only protein-protein interactions imex curation Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress. Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress. Biochem. J. (0264-6021) 2013 imex curation Kern F., Stanika R.I., Sarg B., Offterdinger M., Hess D., Obermair G.J., Lindner H., Bandtlow C.E., Hengst L., Schweigreiter R. Affinomics - Interactions curated for the Affinomics consortium. ruediger.schweigreiter@i-med.ac.at 20-Oct-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-brain_hippocampal_neuron Mus musculus, brain, hippocampal neurons brain-hippocampus_neuron Hippocampal neuron confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Kern F., Stanika R.I., Sarg B., Offterdinger M., Hess D., Obermair G.J., Lindner H., Bandtlow C.E., Hengst L., Schweigreiter R. Affinomics - Interactions curated for the Affinomics consortium. ruediger.schweigreiter@i-med.ac.at Accepted 2014-OCT-07 by ORCHARD Biochem. J. (0264-6021) 2013 Only protein-protein interactions imex curation Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress. Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress. Biochem. J. (0264-6021) 2013 imex curation Kern F., Stanika R.I., Sarg B., Offterdinger M., Hess D., Obermair G.J., Lindner H., Bandtlow C.E., Hengst L., Schweigreiter R. Affinomics - Interactions curated for the Affinomics consortium. ruediger.schweigreiter@i-med.ac.at 20-Oct-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Kern F., Stanika R.I., Sarg B., Offterdinger M., Hess D., Obermair G.J., Lindner H., Bandtlow C.E., Hengst L., Schweigreiter R. Affinomics - Interactions curated for the Affinomics consortium. ruediger.schweigreiter@i-med.ac.at Accepted 2014-OCT-07 by ORCHARD Biochem. J. (0264-6021) 2013 Only protein-protein interactions imex curation Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress. Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress. Biochem. J. (0264-6021) 2013 imex curation Kern F., Stanika R.I., Sarg B., Offterdinger M., Hess D., Obermair G.J., Lindner H., Bandtlow C.E., Hengst L., Schweigreiter R. Affinomics - Interactions curated for the Affinomics consortium. ruediger.schweigreiter@i-med.ac.at 20-Oct-2014: Contacted by IntAct-Help. Only protein-protein interactions crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kern F., Stanika R.I., Sarg B., Offterdinger M., Hess D., Obermair G.J., Lindner H., Bandtlow C.E., Hengst L., Schweigreiter R. Affinomics - Interactions curated for the Affinomics consortium. ruediger.schweigreiter@i-med.ac.at Accepted 2014-OCT-07 by ORCHARD Biochem. J. (0264-6021) 2013 Only protein-protein interactions imex curation hs74l_mouse Heat shock 70 kDa protein 4L Hspa4l Apg1 Hsp4l Osp94 Osmotic stress protein 94 Heat shock 70-related protein APG-1 protein protein mouse Mus musculus Mouse MSVVGIDLGFLNCYIAVARSGGIETIANEYSDRCTPACISLGSRTRAIGNAAKSQIVTNVRNTIHGFKKLHGRSFDDPIVQTERIRLPYELQKMPNGSTGVKVRYLEEERPFAIEQVTGMLLAKLKETSENALKKPVADCVISIPSFFTDAERRSVMAAAQVAGLNCLRLMNETTAVALAYGIYKQDLPSLDEKPRNVVFIDMGHSAYQVSVCAFNKGKLKVLATTFDPYLGGRNFDEALVDYFCDEFKTKYKINVKENSRALLRLYQECEKLKKLMSANASDLPLNIECFMNDLDVSSKMNRAQFEQLCASLLARVEPPLKSVMDQANLQREDINSIEIVGGATRIPAVKEQVTRFFLKDISTTLNADEAVARGCALQCAILSPAFKVREFSITDLVPYSVTLRWKTSFEEGTGECEVFSKNHPAPFSKVITFHKKEPFELEAFYTNLHEVPYPDPRIGNFTIQNVFPQSDGDSSKVKVKVRINIHGIFSVASASVIEKQNLEGDHNDAAMETEAPKSEGKEDVDKMQVDQEEGGHQKCHAEHTPEEEIDHTGAKAKAPPSDKQDRINQTIKKGKIKSIDLPIQSSLYRQLTQDLLNSYIENEGKMIMQDKLEKERNDAKNAVEEYVYDFRDKLGTVYEKFITPEDMNKLSAMLEDTENWLYEEGEDQPKQVYVDRLQELKKYGQPIQMKYVEHEERPKALNDLGKKIQLVLKVIEAHRNKDERYDHLDPAEMERVEKYISDSMNWLNSKMNAQNKLSLTQDPVVKVSEIVTKSKELDNFCNPIVYKPKPKVEAPEDKAKTGSEHNGPMDGQSGSETSPDPPKGSSQHTDSGEMEVD mint Possesses chaperone activity in vitro where it inhibits aggregation of citrate synthase B2C5847DA7EAF6B7 clh1_mouse Clathrin heavy chain 1 Cltc protein protein mouse Mus musculus Mouse MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVVIIDMNDPSNPIRRPISADSAIMNPASKVIALKAGKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGESQPVKMFDRHSSLAGCQIINYRTDAKQKWLLLTGISAQQNRVVGAMQLYSVDRKVSQPIEGHAASFAQFKMEGNAEESTLFCFAVRGQAGGKLHIIEVGTPPTGNQPFPKKAVDVFFPPEAQNDFPVAMQISEKHDVVFLITKYGYIHLYDLETGTCIYMNRISGETIFVTAPHEATAGIIGVNRKGQVLSVCVEEENIIPYITNVLQNPDLALRMAVRNNLAGAEELFARKFNALFAQGNYSEAAKVAANAPKGILRTPDTIRRFQSVPAQPGQTSPLLQYFGILLDQGQLNKYESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYLRANVPNKVIQCFAETGQVQKIVLYAKKVGYTPDWIFLLRNVMRISPDQGQQFAQMLVQDEEPLADITQIVDVFMEYNLIQQCTAFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAILGNQMFTHYDRAHIAQLCEKAGLLQRALEHFTDLYDIKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQICVQVASKYHEQLSTQSLIELFESFKSFEGLFYFLGSIVNFSQDPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQFSTDELVAEVEKRNRLKLLLPWLEARIHEGCEEPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCEKRDPHLACVAYERGQCDLELINVCNENSLFKSLSRYLVRRKDPELWGSVLLESNPYRRPLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISNELFEEAFAIFRKFDVNTSAVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGMVKEAIDSYIKADDPSSYMEVVQAANASGNWEELVKYLQMARKKARESYVETELIFALAKTNRLAELEEFINGPNNAHIQQVGDRCYDEKMYDAAKLLYNNVSNFGRLASTLVHLGEYQAAVDGARKANSTRTWKEVCFACVDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEYDNAIITMMNHPTDAWKEGQFKDIITKVANVELYYKAIQFYLEFKPLLLNDLLMVLSPRLDHTRAVNYFSKVKQLPLVKPYLRSVQNHNNKSVNESLNNLFITEEDYQALRTSIDAYDNFDNISLAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDSLYKDAMQYASESKDTELAEELLQWFLQEEKRECFGACLFTCYDLLRPDVVLETAWRHNIMDFAMPYFIQVMKEYLTKVDKLDASESLRKEEEQATETQPIVYGQPQLMLTAGPSVAVPPQAPFGYGYTAPPYGQPQPGFGYSM DIP protein Q68FD5 original sequence version: 84 6C41EBD89EB7D56B prdx4_mouse Peroxiredoxin-4 Peroxiredoxin IV Thioredoxin peroxidase AO372 Thioredoxin-dependent peroxide reductase A0372 Antioxidant enzyme AOE372 Thioredoxin-dependent peroxiredoxin 4 Prdx4 protein protein mouse Mus musculus Mouse MEARSKLLDGTTASRRWTRKLVLLLPPLLLFLLRTESLQGLESDERFRTRENECHFYAGGQVYPGEASRVSVADHSLHLSKAKISKPAPYWEGTAVINGEFKELKLTDYRGKYLVFFFYPLDFTFVCPTEIIAFGDRIEEFKSINTEVVACSVDSQFTHLAWINTPRRQGGLGPIRIPLLSDLNHQISKDYGVYLEDSGHTLRGLFIIDDKGVLRQITLNDLPVGRSVDETLRLVQAFQYTDKHGEVCPAGWKPGSETIIPDPAGKLKYFDKLN DIP protein O08807 original sequence version: 128 73DB5374EC46241C camk2a calcium/calmodulin-dependent protein kinase II alpha, isoform CRA_a, partial protein protein mouse Mus musculus Mouse MDMHLCAHVSLSPRGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYQQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPSKRITAAEALKHPWISHRSTVASCMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSGGKSGGNKKNDGVKESSESTNTTIEDEDTKVRKQEIIKVTEQLIEAISNGDFESYTKMCDPGMTAFEPEALGNLVEGLDFHRFYFENLWSRNSKPVHTTILNPHIHLMGDESACIAYIRITQYLDAGGIPRTAQSEETRV prdx2_mouse Peroxiredoxin-2 Thioredoxin-dependent peroxiredoxin 2 Thioredoxin peroxidase 1 Thioredoxin-dependent peroxide reductase 1 Thiol-specific antioxidant protein Prdx2 Tdpx1 Tpx protein protein mouse Mus musculus Mouse MASGNAQIGKSAPDFTATAVVDGAFKEIKLSDYRGKYVVLFFYPLDFTFVCPTEIIAFSDHAEDFRKLGCEVLGVSVDSQFTHLAWINTPRKEGGLGPLNIPLLADVTKSLSQNYGVLKNDEGIAYRGLFIIDAKGVLRQITVNDLPVGRSVDEALRLVQAFQYTDEHGEVCPAGWKPGSDTIKPNVDDSKEYFSKHN FE216F5426F7174D prdx5_mouse Peroxiredoxin-5, mitochondrial Prdx5 Prdx6 Peroxiredoxin V Peroxisomal antioxidant enzyme PLP Thioredoxin peroxidase PMP20 Antioxidant enzyme B166 Liver tissue 2D-page spot 2D-0014IV Thioredoxin-dependent peroxiredoxin 5 protein protein mouse Mus musculus Mouse MLQLGLRVLGCKASSVLRASTCLAGRAGRKEAGWECGGARSFSSSAVTMAPIKVGDAIPSVEVFEGEPGKKVNLAELFKGKKGVLFGVPGAFTPGCSKTHLPGFVEQAGALKAKGAQVVACLSVNDVFVIEEWGRAHQAEGKVRLLADPTGAFGKATDLLLDDSLVSLFGNRRLKRFSMVIDNGIVKALNVEPDGTGLTCSLAPNILSQL E944104CC468BDD8 pp1r7_mouse Protein phosphatase 1 regulatory subunit 7 Ppp1r7 Sds22 Protein phosphatase 1 regulatory subunit 22 protein protein mouse Mus musculus Mouse MAAERGAGQQQSQEMMEVDRRVESEESGDEEGKKHGGGGIVANLSEQSLKDGVDRGAEDPEEEHELAVDMETINLDRDAEDVDLTHYRIGKIEGLEVLKKVKSLCLRQNLIKCIENLEELQSLRELDLYDNQIKKIENLEALTELEVLDISFNMLRNIEGIDKLTQLKKLFLVNNKINKIENISNLHQLQMLELGSNRIRAIENIDTLTNLESLFLGKNKITKLQNLDALTNLTVLSVQSNRLAKIEGLQSLVNLRELYLSNNGIEVIEGLENNNKLTMLDIASNRIKKIENISHLTELQEFWMNDNLLESWSDLDELKGARSLETVYLERNPLQKDPQYRRKVMLALPSVRQIDATYVRF mint Regulatory subunit of protein phosphatase 1 A31206AFB3BD0F30 rtn4_mouse Reticulon-4 Rtn4 Kiaa0886 Nogo Neurite outgrowth inhibitor protein protein mouse Mus musculus Mouse MEDIDQSSLVSSSADSPPRPPPAFKYQFVTEPEDEEDEEDEEEEEDDEDLEELEVLERKPAAGLSAAPVPPAAAPLLDFSSDSVPPAPRGPLPAAPPTAPERQPSWERSPAASAPSLPPAAAVLPSKLPEDDEPPARPPAPAGASPLAEPAAPPSTPAAPKRRGSGSVDETLFALPAASEPVIPSSAEKIMDLKEQPGNTVSSGQEDFPSVLFETAASLPSLSPLSTVSFKEHGYLGNLSAVASTEGTIEETLNEASRELPERATNPFVNRESAEFSVLEYSEMGSSFNGSPKGESAMLVENTKEEVIVRSKDKEDLVCSAALHNPQESPATLTKVVKEDGVMSPEKTMDIFNEMKMSVVAPVREEYADFKPFEQAWEVKDTYEGSRDVLAARANMESKVDKKCFEDSLEQKGHGKDSESRNENASFPRTPELVKDGSRAYITCDSFSSATESTAANIFPVLEDHTSENKTDEKKIEERKAQIITEKTSPKTSNPFLVAIHDSEADYVTTDNLSKVTEAVVATMPEGLTPDLVQEACESELNEATGTKIAYETKVDLVQTSEAIQESIYPTAQLCPSFEEAEATPSPVLPDIVMEAPLNSLLPSTGASVAQPSASPLEVPSPVSYDGIKLEPENPPPYEEAMSVALKTSDSKEEIKEPESFNAAAQEAEAPYISIACDLIKETKLSTEPSPEFSNYSEIAKFEKSVPDHCELVDDSSPESEPVDLFSDDSIPEVPQTQEEAVMLMKESLTEVSETVTQHKHKERLSASPQEVGKPYLESFQPNLHITKDAASNEIPTLTKKETISLQMEEFNTAIYSNDDLLSSKEDKMKESETFSDSSPIEIIDEFPTFVSAKDDSPKEYTDLEVSNKSEIANVQSGANSLPCSELPCDLSFKNTYPKDEAHVSDEFSKSRSSVSKVPLLLPNVSALESQIEMGNIVKPKVLTKEAEEKLPSDTEKEDRSLTAVLSAELNKTSVVDLLYWRDIKKTGVVFGASLFLLLSLTVFSIVSVTAYIALALLSVTISFRIYKGVIQAIQKSDEGHPFRAYLESEVAISEELVQKYSNSALGHVNSTIKELRRLFLVDDLVDSLKFAVLMWVFTYVGALFNGLTLLILALISLFSIPVIYERHQAQIDHYLGLANKSVKDAMAKIQAKIPGLKRKAE DIP protein Q99P72 original sequence version: 116 q9jk11-1 Reticulon-4 Nogo-A NI-220-250 Rtn4 Nogo Foocen Glut4 vesicle 20 kDa protein Neurite outgrowth inhibitor protein protein rat Rattus norvegicus (Rat) Rat MEDIDQSSLVSSSTDSPPRPPPAFKYQFVTEPEDEEDEEEEEDEEEDDEDLEELEVLERKPAAGLSAAAVPPAAAAPLLDFSSDSVPPAPRGPLPAAPPAAPERQPSWERSPAAPAPSLPPAAAVLPSKLPEDDEPPARPPPPPPAGASPLAEPAAPPSTPAAPKRRGSGSVDETLFALPAASEPVIPSSAEKIMDLMEQPGNTVSSGQEDFPSVLLETAASLPSLSPLSTVSFKEHGYLGNLSAVSSSEGTIEETLNEASKELPERATNPFVNRDLAEFSELEYSEMGSSFKGSPKGESAILVENTKEEVIVRSKDKEDLVCSAALHSPQESPVGKEDRVVSPEKTMDIFNEMQMSVVAPVREEYADFKPFEQAWEVKDTYEGSRDVLAARANVESKVDRKCLEDSLEQKSLGKDSEGRNEDASFPSTPEPVKDSSRAYITCASFTSATESTTANTFPLLEDHTSENKTDEKKIEERKAQIITEKTSPKTSNPFLVAVQDSEADYVTTDTLSKVTEAAVSNMPEGLTPDLVQEACESELNEATGTKIAYETKVDLVQTSEAIQESLYPTAQLCPSFEEAEATPSPVLPDIVMEAPLNSLLPSAGASVVQPSVSPLEAPPPVSYDSIKLEPENPPPYEEAMNVALKALGTKEGIKEPESFNAAVQETEAPYISIACDLIKETKLSTEPSPDFSNYSEIAKFEKSVPEHAELVEDSSPESEPVDLFSDDSIPEVPQTQEEAVMLMKESLTEVSETVAQHKEERLSASPQELGKPYLESFQPNLHSTKDAASNDIPTLTKKEKISLQMEEFNTAIYSNDDLLSSKEDKIKESETFSDSSPIEIIDEFPTFVSAKDDSPKLAKEYTDLEVSDKSEIANIQSGADSLPCLELPCDLSFKNIYPKDEVHVSDEFSENRSSVSKASISPSNVSALEPQTEMGSIVKSKSLTKEAEKKLPSDTEKEDRSLSAVLSAELSKTSVVDLLYWRDIKKTGVVFGASLFLLLSLTVFSIVSVTAYIALALLSVTISFRIYKGVIQAIQKSDEGHPFRAYLESEVAISEELVQKYSNSALGHVNSTIKELRRLFLVDDLVDSLKFAVLMWVFTYVGALFNGLTLLILALISLFSIPVIYERHQVQIDHYLGLANKSVKDAMAKIQAKIPGLKRKAD DIP protein Q9JK11-1 original sequence version: 97 8CB894B09E94F0B6 caza2_mouse F-actin-capping protein subunit alpha-2 Capza2 Cappa2 CapZ alpha-2 protein protein mouse Mus musculus Mouse MADLEEQLSDEEKVRIAAKFIIHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNLDQFTPVKIEGYEDQVLITEHGDLGNGKFLDPKNRICFKFDHLRKEATDPRPYEAENAIESWRTSVETALRAYVKEHYPNGVCTVYGKKVDGQQTIIACIESHQFQAKNFWNGRWRSEWKFTVTPSTTQVVGILKIQVHYYEDGNVQLVSHKDIQDSLTVSNEVQTAKEFIKIVEAAENEYQTAISENYQTMSDTTFKALRRQLPVTRTKIDWNKILSYKIGKEMQNA DIP protein P47754 original sequence version: 85 E706A9BC1830E70B prdx1_mouse Peroxiredoxin-1 Thioredoxin-dependent peroxiredoxin 1 Thioredoxin-dependent peroxide reductase 2 Osteoblast-specific factor 3 Macrophage 23 kDa stress protein Thioredoxin peroxidase 2 Prdx1 Msp23 Paga Tdpx2 protein protein mouse Mus musculus Mouse MSSGNAKIGYPAPNFKATAVMPDGQFKDISLSEYKGKYVVFFFYPLDFTFVCPTEIIAFSDRADEFKKLNCQVIGASVDSHFCHLAWINTPKKQGGLGPMNIPLISDPKRTIAQDYGVLKADEGISFRGLFIIDDKGILRQITINDLPVGRSVDEIIRLVQAFQFTDKHGEVCPAGWKPGSDTIKPDVNKSKEYFSKQK BEF5C995A86124D1 q8bz98-2 Dynamin-3 Dnm3 Kiaa0820 protein protein mouse Mus musculus Mouse MGNREMEELIPLVNRLQDAFSALGQSCLLELPQIAVVGGQSAGKSSVLENFVGRDFLPRGSGIVTRRPLVLQLVTSKAEYAEFLHCKGKKFTDFDEVRHEIEAETDRVTGMNKGISSIPINLRVYSPHVLNLTLIDLPGITKVPVGDQPPDIEYQIRDMIMQFITRENCLILAVTPANTDLANSDALKLAKEVDPQGLRTIGVITKLDLMDEGTDARDVLENKLLPLRRGYVGVVNRSQKDIDGKKDIKAAMLAERKFFLSHPAYRHIADRMGTPHLQKVLNQQLTNHIRDTLPNFRNKLQGQLLSIEHEVEAFKNFKPEDPTRKTKALLQMVQQFAVDFEKRIEGSGDQVDTLELSGGAKINRIFHERFPFEIVKMEFNEKELRREISYAIKNIHGIRTGLFTPDMAFEAIVKKQIVKLKGPSLKSVDLVMQELINTVKKCTKRLANFPRLCEETERIVANHIREREGKTKDQVLLLIDIQVSYINTNHEDFIGFANAQQRSSQVHKKSTIGNQVIRKGWLTVSNIGIMKGGSKGYWFVLTAESLSWYKDDEEKEKKYMLPLDNLKVRDVEKGFMSSKHVFALFNTEQRNVYKDYRFLELACDSQEDVDSWKASLLRAGVYPDKSVTENDENGQAENFSMDPQLERQVETIRNLVDSYMSIINKCIRDLIPKTIMHLMINNVKDFINSELLAQLYSSEDQNTLMEESAEQAQRRDEMLRMYQALKEALAIIGDINTATVSTPAPPPVDDSWLQHSRRSPPPSPTTQRRLTISAPLPRPTSGRGPAPAIPSPGPHSGAPPVPFRPGPLPPFPNSSDSFGAPPQVPSRPTRAPPSVPSRRPPPSPTRPTIIRPLESSLLD No experimental confirmation available. 591E1B65A81A26D0 rtn4-hspa4l-3 3 8 unspecified role unspecified role prey prey mouse-brain Mus musculus brain brain Brain [cerebrum] 13 unspecified role unspecified role prey prey mouse-brain Mus musculus brain brain Brain [cerebrum] 12 unspecified role unspecified role prey prey mouse-brain Mus musculus brain brain Brain [cerebrum] 18 unspecified role unspecified role prey prey mouse-brain Mus musculus brain brain Brain [cerebrum] 16 unspecified role unspecified role bait bait nig_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 association association Fig 1C, Supp table 1 TCA precipitation was used prior to MS/MS. All proteins listed were identified in two independent MS screens. rtn4-camk2a-6 3 14 unspecified role unspecified role prey prey mouse-brain Mus musculus brain brain Brain [cerebrum] 8 unspecified role unspecified role prey prey mouse-brain Mus musculus brain brain Brain [cerebrum] 17 unspecified role unspecified role prey prey mouse-brain Mus musculus brain brain Brain [cerebrum] 9 unspecified role unspecified role prey prey mouse-brain Mus musculus brain brain Brain [cerebrum] 16 unspecified role unspecified role bait bait c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range nig_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 10 unspecified role unspecified role prey prey mouse-brain Mus musculus brain brain Brain [cerebrum] 18 unspecified role unspecified role prey prey mouse-brain Mus musculus brain brain Brain [cerebrum] 11 unspecified role unspecified role prey prey mouse-brain Mus musculus brain brain Brain [cerebrum] 19 unspecified role unspecified role prey prey mouse-brain Mus musculus brain brain Brain [cerebrum] association association 1C, Supp table 1 SDS/PAGE, followed by in-gel digestion was used prior to MS/MS I have created a new protein for CaMKIIα/EDL09793.1 as the only matches in UniProt omit the aas ~1-14 (N-terminus) from EDL09793.1 completely -this protein has a different N terminus from anything in UniProt. All other EDL identifiers given partial matches only - but there is ~100% identity in overlapping regions and no more than 1/2 mismatches. rtn4-hspa4l-4 6 8 unspecified role unspecified role prey prey mouse-brain Mus musculus brain brain Brain [cerebrum] 16 unspecified role unspecified role bait bait nig_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 physical association physical association Fig 1D very faint band visible rtn4-hspa4l-5 7 8 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait physical association physical association Fig 1E hspa4l-rtn4 7 8 unspecified role unspecified role bait bait 16 unspecified role unspecified role prey prey physical association physical association Fig 1E hspa4l-rtn4-1 4 8 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey physical association physical association Fig 2A rtn4-hspa4l-6 4 8 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait physical association physical association Fig 2A hspa4l-rtn4-4 5 8 unspecified role unspecified role neutral component neutral component 15 unspecified role unspecified role neutral component neutral component colocalization colocalization Supp Fig 1 hspa4l-rtn4-3 1 8 unspecified role unspecified role neutral component neutral component nls_from_sv40_large-t-antigen tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range e_blue_fluorescent_protein fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 16 unspecified role unspecified role neutral component neutral component c_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction Fig 4A hspa4l-rtn4-5 2 8 unspecified role unspecified role neutral component neutral component 15 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction Fig 4B