IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Association of the protein-tyrosine phosphatase DEP-1 with its substrate FLT3 visualized by in situ proximity ligation assay. Association of the protein-tyrosine phosphatase DEP-1 with its substrate FLT3 visualized by in situ proximity ligation assay. PLoS ONE 2013 imex curation Böhmer S.A., Weibrecht I., Söderberg O., Böhmer F.D. Affinomics - Interactions curated for the Affinomics consortium. I assume that Thp-1 cells are used throughout as they look nowt like COS7 cells.(fig1) boehmer@med.uni-jena.de 20-Oct-2014: Contacted by IntAct-Help. Only protein-protein interactions human-mv4-11 Human MV4-11, Acute Monocytic Leukemia cell line mv4-11 MV4-11 a human MLL leukemia cell line https://www.lgcstandards-atcc.org/Products/All/CRL-9591.aspx Human acute monocytic leukemia cell line. Described to carry a 4;11 translocation; MV4-11 carries an FLT3 internal tandem duplication. pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Böhmer S.A., Weibrecht I., Söderberg O., Böhmer F.D. Affinomics - Interactions curated for the Affinomics consortium. I assume that Thp-1 cells are used throughout as they look nowt like COS7 cells.(fig1) boehmer@med.uni-jena.de Accepted 2014-OCT-07 by ORCHARD PLoS ONE 2013 Only protein-protein interactions imex curation Association of the protein-tyrosine phosphatase DEP-1 with its substrate FLT3 visualized by in situ proximity ligation assay. Association of the protein-tyrosine phosphatase DEP-1 with its substrate FLT3 visualized by in situ proximity ligation assay. PLoS ONE 2013 imex curation Böhmer S.A., Weibrecht I., Söderberg O., Böhmer F.D. Affinomics - Interactions curated for the Affinomics consortium. I assume that Thp-1 cells are used throughout as they look nowt like COS7 cells.(fig1) boehmer@med.uni-jena.de 20-Oct-2014: Contacted by IntAct-Help. Only protein-protein interactions human-thp-1 Homo sapiens acute monocytic leukemia cells thp-1 Human acute monocytic leukemia cell line pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Böhmer S.A., Weibrecht I., Söderberg O., Böhmer F.D. Affinomics - Interactions curated for the Affinomics consortium. I assume that Thp-1 cells are used throughout as they look nowt like COS7 cells.(fig1) boehmer@med.uni-jena.de Accepted 2014-OCT-07 by ORCHARD PLoS ONE 2013 Only protein-protein interactions imex curation Association of the protein-tyrosine phosphatase DEP-1 with its substrate FLT3 visualized by in situ proximity ligation assay. Association of the protein-tyrosine phosphatase DEP-1 with its substrate FLT3 visualized by in situ proximity ligation assay. PLoS ONE 2013 imex curation Böhmer S.A., Weibrecht I., Söderberg O., Böhmer F.D. Affinomics - Interactions curated for the Affinomics consortium. I assume that Thp-1 cells are used throughout as they look nowt like COS7 cells.(fig1) boehmer@med.uni-jena.de 20-Oct-2014: Contacted by IntAct-Help. Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Böhmer S.A., Weibrecht I., Söderberg O., Böhmer F.D. Affinomics - Interactions curated for the Affinomics consortium. I assume that Thp-1 cells are used throughout as they look nowt like COS7 cells.(fig1) boehmer@med.uni-jena.de Accepted 2014-OCT-07 by ORCHARD PLoS ONE 2013 Only protein-protein interactions imex curation flt3_human Receptor-type tyrosine-protein kinase FLT3 FLK2 FL cytokine receptor Fetal liver kinase-2 FLT3 STK1 Fms-like tyrosine kinase 3 Stem cell tyrosine kinase 1 CD135 protein protein human Homo sapiens Human MPALARDGGQLPLLVVFSAMIFGTITNQDLPVIKCVLINHKNNDSSVGKSSSYPMVSESPEDLGCALRPQSSGTVYEAAAVEVDVSASITLQVLVDAPGNISCLWVFKHSSLNCQPHFDLQNRGVVSMVILKMTETQAGEYLLFIQSEATNYTILFTVSIRNTLLYTLRRPYFRKMENQDALVCISESVPEPIVEWVLCDSQGESCKEESPAVVKKEEKVLHELFGTDIRCCARNELGRECTRLFTIDLNQTPQTTLPQLFLKVGEPLWIRCKAVHVNHGFGLTWELENKALEEGNYFEMSTYSTNRTMIRILFAFVSSVARNDTGYYTCSSSKHPSQSALVTIVEKGFINATNSSEDYEIDQYEEFCFSVRFKAYPQIRCTWTFSRKSFPCEQKGLDNGYSISKFCNHKHQPGEYIFHAENDDAQFTKMFTLNIRRKPQVLAEASASQASCFSDGYPLPSWTWKKCSDKSPNCTEEITEGVWNRKANRKVFGQWVSSSTLNMSEAIKGFLVKCCAYNSLGTSCETILLNSPGPFPFIQDNISFYATIGVCLLFIVVLTLLICHKYKKQFRYESQLQMVQVTGSSDNEYFYVDFREYEYDLKWEFPRENLEFGKVLGSGAFGKVMNATAYGISKTGVSIQVAVKMLKEKADSSEREALMSELKMMTQLGSHENIVNLLGACTLSGPIYLIFEYCCYGDLLNYLRSKREKFHRTWTEIFKEHNFSFYPTFQSHPNSSMPGSREVQIHPDSDQISGLHGNSFHSEDEIEYENQKRLEEEEDLNVLTFEDLLCFAYQVAKGMEFLEFKSCVHRDLAARNVLVTHGKVVKICDFGLARDIMSDSNYVVRGNARLPVKWMAPESLFEGIYTIKSDVWSYGILLWEIFSLGVNPYPGIPVDANFYKLIQNGFKMDQPFYATEEIYIIMQSCWAFDSRKRPSFPNLTSFLGCQLADAEEAMYQNVDGRVSECPHTYQNRRPFSREMDLGLLSPQAQVEDS DIP protein P36888 original sequence version: 118 ptprj_human Receptor-type tyrosine-protein phosphatase eta PTPRJ DEP1 HPTP eta Protein-tyrosine phosphatase receptor type J Density-enhanced phosphatase 1 protein protein human Homo sapiens Human MKPAAREARLPPRSPGLRWALPLLLLLLRLGQILCAGGTPSPIPDPSVATVATGENGITQISSTAESFHKQNGTGTPQVETNTSEDGESSGANDSLRTPEQGSNGTDGASQKTPSSTGPSPVFDIKAVSISPTNVILTWKSNDTAASEYKYVVKHKMENEKTITVVHQPWCNITGLRPATSYVFSITPGIGNETWGDPRVIKVITEPIPVSDLRVALTGVRKAALSWSNGNGTASCRVLLESIGSHEELTQDSRLQVNISGLKPGVQYNINPYLLQSNKTKGDPLGTEGGLDASNTERSRAGSPTAPVHDESLVGPVDPSSGQQSRDTEVLLVGLEPGTRYNATVYSQAANGTEGQPQAIEFRTNAIQVFDVTAVNISATSLTLIWKVSDNESSSNYTYKIHVAGETDSSNLNVSEPRAVIPGLRSSTFYNITVCPVLGDIEGTPGFLQVHTPPVPVSDFRVTVVSTTEIGLAWSSHDAESFQMHITQEGAGNSRVEITTNQSIIIGGLFPGTKYCFEIVPKGPNGTEGASRTVCNRTVPSAVFDIHVVYVTTTEMWLDWKSPDGASEYVYHLVIESKHGSNHTSTYDKAITLQGLIPGTLYNITISPEVDHVWGDPNSTAQYTRPSNVSNIDVSTNTTAATLSWQNFDDASPTYSYCLLIEKAGNSSNATQVVTDIGITDATVTELIPGSSYTVEIFAQVGDGIKSLEPGRKSFCTDPASMASFDCEVVPKEPALVLKWTCPPGANAGFELEVSSGAWNNATHLESCSSENGTEYRTEVTYLNFSTSYNISITTVSCGKMAAPTRNTCTTGITDPPPPDGSPNITSVSHNSVKVKFSGFEASHGPIKAYAVILTTGEAGHPSADVLKYTYEDFKKGASDTYVTYLIRTEEKGRSQSLSEVLKYEIDVGNESTTLGYYNGKLEPLGSYRACVAGFTNITFHPQNKGLIDGAESYVSFSRYSDAVSLPQDPGVICGAVFGCIFGALVIVTVGGFIFWRKKRKDAKNNEVSFSQIKPKKSKLIRVENFEAYFKKQQADSNCGFAEEYEDLKLVGISQPKYAAELAENRGKNRYNNVLPYDISRVKLSVQTHSTDDYINANYMPGYHSKKDFIATQGPLPNTLKDFWRMVWEKNVYAIIMLTKCVEQGRTKCEEYWPSKQAQDYGDITVAMTSEIVLPEWTIRDFTVKNIQTSESHPLRQFHFTSWPDHGVPDTTDLLINFRYLVRDYMKQSPPESPILVHCSAGVGRTGTFIAIDRLIYQIENENTVDVYGIVYDLRMHRPLMVQTEDQYVFLNQCVLDIVRSQKDSKVDLIYQNTTAMTIYENLAPVTTFGKTNGYIA B44F4343FC8FD1B4 flt3-ptprj 3 4 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component Q12913:p.Cys1239Ser mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain C S Wed May 02 17:00:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys1239ser' Tue Jun 30 19:36:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys1239Ser' proximity proximity close-contact colocalization neighbourhood interaction 1A flt3-ptprj-1 2 5 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 1B/C, 2A, 3 FLT3 ligand Compound 102 -a FLT3 kinase inhibitor Hydrogen peroxide flt3-ptprj-2 1 4 unspecified role unspecified role neutral component neutral component undefined mutation mutation mutation undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Wed Jul 06 17:10:37 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'internal_tandem_duplication' 5 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 3B weak interaction Internal tandem duplications (ITD) of amino acid stretches can be found in the juxtamembrane domain or in the tyrosine kinase domain;this mutation confers cytokine-independent proliferation and resistance to apoptosis.