IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Protein phosphorylation profiling using an in situ proximity ligation assay: phosphorylation of AURKA-elicited EGFR-Thr654 and EGFR-Ser1046 in lung cancer cells. Protein phosphorylation profiling using an in situ proximity ligation assay: phosphorylation of AURKA-elicited EGFR-Thr654 and EGFR-Ser1046 in lung cancer cells. PLoS ONE 2013 imex curation Chen T.C., Liu Y.W., Huang Y.H., Yeh Y.C., Chou T.Y., Wu Y.C., Wu C.C., Chen Y.R., Cheng H.C., Lu P.J., Lai J.M., Huang C.Y. Affinomics - Interactions curated for the Affinomics consortium. cyhuang5@ym.edu.tw 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions human-h1975 Homo sapiens h1975 Human adenocarcinoma; non-small cell lung cancer H-175 pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Chen T.C., Liu Y.W., Huang Y.H., Yeh Y.C., Chou T.Y., Wu Y.C., Wu C.C., Chen Y.R., Cheng H.C., Lu P.J., Lai J.M., Huang C.Y. Affinomics - Interactions curated for the Affinomics consortium. cyhuang5@ym.edu.tw Accepted 2014-AUG-05 by ORCHARD PLoS ONE 2013 Only protein-protein interactions imex curation Protein phosphorylation profiling using an in situ proximity ligation assay: phosphorylation of AURKA-elicited EGFR-Thr654 and EGFR-Ser1046 in lung cancer cells. Protein phosphorylation profiling using an in situ proximity ligation assay: phosphorylation of AURKA-elicited EGFR-Thr654 and EGFR-Ser1046 in lung cancer cells. PLoS ONE 2013 imex curation Chen T.C., Liu Y.W., Huang Y.H., Yeh Y.C., Chou T.Y., Wu Y.C., Wu C.C., Chen Y.R., Cheng H.C., Lu P.J., Lai J.M., Huang C.Y. Affinomics - Interactions curated for the Affinomics consortium. cyhuang5@ym.edu.tw 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Chen T.C., Liu Y.W., Huang Y.H., Yeh Y.C., Chou T.Y., Wu Y.C., Wu C.C., Chen Y.R., Cheng H.C., Lu P.J., Lai J.M., Huang C.Y. Affinomics - Interactions curated for the Affinomics consortium. cyhuang5@ym.edu.tw Accepted 2014-AUG-05 by ORCHARD PLoS ONE 2013 Only protein-protein interactions imex curation Protein phosphorylation profiling using an in situ proximity ligation assay: phosphorylation of AURKA-elicited EGFR-Thr654 and EGFR-Ser1046 in lung cancer cells. Protein phosphorylation profiling using an in situ proximity ligation assay: phosphorylation of AURKA-elicited EGFR-Thr654 and EGFR-Ser1046 in lung cancer cells. PLoS ONE 2013 imex curation Chen T.C., Liu Y.W., Huang Y.H., Yeh Y.C., Chou T.Y., Wu Y.C., Wu C.C., Chen Y.R., Cheng H.C., Lu P.J., Lai J.M., Huang C.Y. Affinomics - Interactions curated for the Affinomics consortium. cyhuang5@ym.edu.tw 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Chen T.C., Liu Y.W., Huang Y.H., Yeh Y.C., Chou T.Y., Wu Y.C., Wu C.C., Chen Y.R., Cheng H.C., Lu P.J., Lai J.M., Huang C.Y. Affinomics - Interactions curated for the Affinomics consortium. cyhuang5@ym.edu.tw Accepted 2014-AUG-05 by ORCHARD PLoS ONE 2013 Only protein-protein interactions imex curation Protein phosphorylation profiling using an in situ proximity ligation assay: phosphorylation of AURKA-elicited EGFR-Thr654 and EGFR-Ser1046 in lung cancer cells. Protein phosphorylation profiling using an in situ proximity ligation assay: phosphorylation of AURKA-elicited EGFR-Thr654 and EGFR-Ser1046 in lung cancer cells. PLoS ONE 2013 imex curation Chen T.C., Liu Y.W., Huang Y.H., Yeh Y.C., Chou T.Y., Wu Y.C., Wu C.C., Chen Y.R., Cheng H.C., Lu P.J., Lai J.M., Huang C.Y. Affinomics - Interactions curated for the Affinomics consortium. cyhuang5@ym.edu.tw 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein array protein array predetermined predetermined participant predetermined Chen T.C., Liu Y.W., Huang Y.H., Yeh Y.C., Chou T.Y., Wu Y.C., Wu C.C., Chen Y.R., Cheng H.C., Lu P.J., Lai J.M., Huang C.Y. Affinomics - Interactions curated for the Affinomics consortium. Details about this assay are sparse - can find nothing relevant in Ref 44 (uses biotinylated calmodulin)- suppliers site is less than informative, so Participant Detection must be a "Trade-secret"? I have entered the "Bait" as AURKA - but this is a little dubious...? Proteins (clones selected from Invitrogens Ultimate ORF™ Collection*) are expressed as N-terminal GST fusion proteins using a baculovirus expression system. cyhuang5@ym.edu.tw Accepted 2014-AUG-05 by ORCHARD PLoS ONE 2013 Only protein-protein interactions imex curation egfr_human Epidermal growth factor receptor ERBB1 EGFR Receptor tyrosine-protein kinase erbB-1 Proto-oncogene c-ErbB-1 ERBB HER1 protein protein human Homo sapiens Human MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA DIP protein P00533 original sequence version: 213 DIP protein P00533 original sequence version: 214 DIP protein P00533 original sequence version: 119 DIP protein P00533 original sequence version: 207 DIP protein P00533 original sequence version: 132 DIP protein P00533-1 original sequence version: 197 DIP protein P00533 original sequence version: 163 DIP protein P00533-1 original sequence version: 159 DIP protein P00533 original sequence version: 184 DIP protein P00533-1 original sequence version: 174 DIP protein P00533-1 original sequence version: 191 DIP protein P00533 original sequence version: 218 DIP protein P00533 original sequence version: 221 DIP protein P00533-1 original sequence version: 221 DIP protein P00533 original sequence version: 226 DIP protein P00533 original sequence version: 166 DIP protein P00533-1 original sequence version: 166 DIP protein P00533 original sequence version: 194 DIP protein P00533-1 original sequence version: 194 DIP protein P00533-1 original sequence version: 224 DIP protein P00533 original sequence version: 189 DIP protein P00533-1 original sequence version: 207 DIP protein P00533 original sequence version: 177 DIP protein P00533 original sequence version: 168 D8A2A50B4EFB6ED2 aurka_human Aurora kinase A AIRK1 Serine/threonine-protein kinase 15 Aurora/IPL1-related kinase 1 Breast tumor-amplified kinase STK6 AIK ARK1 AURA BTAK STK15 AURKA AYK1 IAK1 Aurora 2 Serine/threonine-protein kinase 6 Serine/threonine-protein kinase aurora-A protein protein human Homo sapiens Human MDRSKENCISGPVKATAPVGGPKRVLVTQQFPCQNPLPVNSGQAQRVLCPSNSSQRVPLQAQKLVSSHKPVQNQKQKQLQATSVPHPVSRPLNNTQKSKQPLPSAPENNPEEELASKQKNEESKKRQWALEDFEIGRPLGKGKFGNVYLAREKQSKFILALKVLFKAQLEKAGVEHQLRREVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGTVYRELQKLSKFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLGSAGELKIADFGWSVHAPSSRRTTLCGTLDYLPPEMIEGRMHDEKVDLWSLGVLCYEFLVGKPPFEANTYQETYKRISRVEFTFPDFVTEGARDLISRLLKHNPSQRPMLREVLEHPWITANSSKPSNCQNKESASKQS DIP protein O14965 original sequence version: 181 DIP protein O14965 original sequence version: 146 DIP protein O14965 original sequence version: 124 DIP protein O14965 original sequence version: 160 DIP protein O14965 original sequence version: 138 DIP protein O14965 original sequence version: 98 125F3594834CD157 egfr-aurka 4 5 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P00533:p.Leu861Gln mutation mutation certain certain sequence position certain certain certain sequence position certain L Q Wed May 02 16:32:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu861gln' Tue Jun 30 20:13:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu861Gln' 6 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 4A AURKA interacted with the EGFR-L861Q mutant, but not EGFR-WT aurka-egfr 3 6 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4B both EGFR-WT and mutated EGFR associates with AURKA-WT and AURKA-KD. aurka-egfr-1 2 6 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 4C EGF aurka-egfr-2 1 5 unspecified role unspecified role neutral component neutral component P00533:p.[Thr790Met;Leu858Arg] mutation mutation certain certain sequence position certain certain certain sequence position certain L R certain certain sequence position certain certain certain sequence position certain T M Fri Jul 22 11:15:04 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'leu858arg,thr790met' Wed May 02 15:44:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr790met,leu858arg' Tue Jun 30 17:45:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Thr790Met;Leu858Arg]' 6 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined proximity proximity close-contact colocalization neighbourhood interaction 4C EGF EGFR-AURKA interaction only occurred after EGF stimulation.