IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Beta-catenin regulates parathyroid hormone/parathyroid hormone-related protein receptor signals and chondrocyte hypertrophy through binding to the intracellular C-terminal region of the receptor. Beta-catenin regulates parathyroid hormone/parathyroid hormone-related protein receptor signals and chondrocyte hypertrophy through binding to the intracellular C-terminal region of the receptor. Arthritis Rheum. (0004-3591) 2013 imex curation Yano F., Saito T., Ogata N., Yamazawa T., Iino M., Chung U.I., Kawaguchi H. Affinomics - Interactions curated for the Affinomics consortium. kawaguchi-ort@h.u-tokyo.ac.jp 30-Sept-2013: Contacted by IntAct-Help. Human kidney cDNA library Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Yano F., Saito T., Ogata N., Yamazawa T., Iino M., Chung U.I., Kawaguchi H. Affinomics - Interactions curated for the Affinomics consortium. kawaguchi-ort@h.u-tokyo.ac.jp Accepted 2013-SEP-13 by MGT 30-Sept-2013: Contacted by IntAct-Help. Human kidney cDNA library Arthritis Rheum. (0004-3591) 2013 Only protein-protein interactions imex curation Beta-catenin regulates parathyroid hormone/parathyroid hormone-related protein receptor signals and chondrocyte hypertrophy through binding to the intracellular C-terminal region of the receptor. Beta-catenin regulates parathyroid hormone/parathyroid hormone-related protein receptor signals and chondrocyte hypertrophy through binding to the intracellular C-terminal region of the receptor. Arthritis Rheum. (0004-3591) 2013 imex curation Yano F., Saito T., Ogata N., Yamazawa T., Iino M., Chung U.I., Kawaguchi H. Affinomics - Interactions curated for the Affinomics consortium. kawaguchi-ort@h.u-tokyo.ac.jp 30-Sept-2013: Contacted by IntAct-Help. Human kidney cDNA library Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Yano F., Saito T., Ogata N., Yamazawa T., Iino M., Chung U.I., Kawaguchi H. Affinomics - Interactions curated for the Affinomics consortium. kawaguchi-ort@h.u-tokyo.ac.jp Accepted 2013-SEP-13 by MGT 30-Sept-2013: Contacted by IntAct-Help. Human kidney cDNA library Arthritis Rheum. (0004-3591) 2013 Only protein-protein interactions imex curation Beta-catenin regulates parathyroid hormone/parathyroid hormone-related protein receptor signals and chondrocyte hypertrophy through binding to the intracellular C-terminal region of the receptor. Beta-catenin regulates parathyroid hormone/parathyroid hormone-related protein receptor signals and chondrocyte hypertrophy through binding to the intracellular C-terminal region of the receptor. Arthritis Rheum. (0004-3591) 2013 imex curation Yano F., Saito T., Ogata N., Yamazawa T., Iino M., Chung U.I., Kawaguchi H. Affinomics - Interactions curated for the Affinomics consortium. kawaguchi-ort@h.u-tokyo.ac.jp 30-Sept-2013: Contacted by IntAct-Help. Human kidney cDNA library Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Yano F., Saito T., Ogata N., Yamazawa T., Iino M., Chung U.I., Kawaguchi H. Affinomics - Interactions curated for the Affinomics consortium. kawaguchi-ort@h.u-tokyo.ac.jp Accepted 2013-SEP-13 by MGT 30-Sept-2013: Contacted by IntAct-Help. Human kidney cDNA library Arthritis Rheum. (0004-3591) 2013 Only protein-protein interactions imex curation Beta-catenin regulates parathyroid hormone/parathyroid hormone-related protein receptor signals and chondrocyte hypertrophy through binding to the intracellular C-terminal region of the receptor. Beta-catenin regulates parathyroid hormone/parathyroid hormone-related protein receptor signals and chondrocyte hypertrophy through binding to the intracellular C-terminal region of the receptor. Arthritis Rheum. (0004-3591) 2013 imex curation Yano F., Saito T., Ogata N., Yamazawa T., Iino M., Chung U.I., Kawaguchi H. Affinomics - Interactions curated for the Affinomics consortium. kawaguchi-ort@h.u-tokyo.ac.jp 30-Sept-2013: Contacted by IntAct-Help. Human kidney cDNA library Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Yano F., Saito T., Ogata N., Yamazawa T., Iino M., Chung U.I., Kawaguchi H. Affinomics - Interactions curated for the Affinomics consortium. kawaguchi-ort@h.u-tokyo.ac.jp Accepted 2013-SEP-13 by MGT 30-Sept-2013: Contacted by IntAct-Help. Human kidney cDNA library Arthritis Rheum. (0004-3591) 2013 Only protein-protein interactions imex curation Beta-catenin regulates parathyroid hormone/parathyroid hormone-related protein receptor signals and chondrocyte hypertrophy through binding to the intracellular C-terminal region of the receptor. Beta-catenin regulates parathyroid hormone/parathyroid hormone-related protein receptor signals and chondrocyte hypertrophy through binding to the intracellular C-terminal region of the receptor. Arthritis Rheum. (0004-3591) 2013 imex curation Yano F., Saito T., Ogata N., Yamazawa T., Iino M., Chung U.I., Kawaguchi H. Affinomics - Interactions curated for the Affinomics consortium. kawaguchi-ort@h.u-tokyo.ac.jp 30-Sept-2013: Contacted by IntAct-Help. Human kidney cDNA library Only protein-protein interactions mouse-chondrocytes Mus musculus chondrocyte chondrocyte fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Yano F., Saito T., Ogata N., Yamazawa T., Iino M., Chung U.I., Kawaguchi H. Affinomics - Interactions curated for the Affinomics consortium. kawaguchi-ort@h.u-tokyo.ac.jp Accepted 2013-SEP-13 by MGT 30-Sept-2013: Contacted by IntAct-Help. Human kidney cDNA library Arthritis Rheum. (0004-3591) 2013 Only protein-protein interactions imex curation pth1r_mouse Parathyroid hormone/parathyroid hormone-related peptide receptor Pth1r Pthr Pthr1 PTH/PTHrP type I receptor Parathyroid hormone 1 receptor protein protein mouse Mus musculus Mouse MGTARIAPSLALLLCCPVLSSAYALVDADDVFTKEEQIFLLHRAQAQCDKLLKEVLHTAANIMESDKGWTPASTSGKPRKEKAPGKFYPESKENKDVPTGSRRRGRPCLPEWDNIVCWPLGAPGEVVAVPCPDYIYDFNHKGHAYRRCDRNGSWEVVPGHNRTWANYSECLKFMTNETREREVFDRLGMIYTVGYSMSLASLTVAVLILAYFRRLHCTRNYIHMHMFLSFMLRAASIFVKDAVLYSGFTLDEAERLTEEELHIIAQVPPPPAAAAVGYAGCRVAVTFFLYFLATNYYWILVEGLYLHSLIFMAFFSEKKYLWGFTIFGWGLPAVFVAVWVGVRATLANTGCWDLSSGHKKWIIQVPILASVVLNFILFINIIRVLATKLRETNAGRCDTRQQYRKLLRSTLVLVPLFGVHYTVFMALPYTEVSGTLWQIQMHYEMLFNSFQGFFVAIIYCFCNGEVQAEIRKSWSRWTLALDFKRKARSGSSSYSYGPMVSHTSVTNVGPRAGLSLPLSPRLLPATTNGHSQLPGHAKPGAPAIENETIPVTMTVPKDDGFLNGSCSGLDEEASGSARPPPLLQEEWETVM C2E6CCAF2ABAFEEF ctnb1_mouse Catenin beta-1 Beta-catenin Ctnnb1 Catnb protein protein mouse Mus musculus Mouse MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEALGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL DIP protein Q02248 original sequence version: 128 DIP protein Q02248 original sequence version: 135 DIP protein Q02248 original sequence version: 147 DIP protein Q02248 original sequence version: 187 DIP protein Q02248 original sequence version: 140 D708F170A3FBED6E ctnb1_human Catenin beta-1 Beta-catenin OK/SW-cl.35 PRO2286 CTNNB1 CTNNB protein protein human Homo sapiens Human MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL DIP protein P35222 original sequence version: 187 DIP protein P35222 original sequence version: 188 DIP protein P35222 original sequence version: 180 DIP protein P35222-1 original sequence version: 181 DIP protein P35222 original sequence version: 181 DIP protein P35222 original sequence version: 107 DIP protein P35222-1 original sequence version: 107 DIP protein P35222 original sequence version: 103 DIP protein P35222-1 original sequence version: 103 DIP protein P35222 original sequence version: 175 DIP protein P35222 original sequence version: 136 DIP protein P35222 original sequence version: 135 DIP protein P35222 original sequence version: 131 DIP protein P35222-1 original sequence version: 133 DIP protein P35222 original sequence version: 133 DIP protein P35222 original sequence version: 148 DIP protein P35222-1 original sequence version: 139 DIP protein P35222 original sequence version: 139 DIP protein P35222 original sequence version: 146 DIP protein P35222 original sequence version: 196 DIP protein P35222 original sequence version: 192 DIP protein P35222 original sequence version: 185 DIP protein P35222 original sequence version: 151 DIP protein P35222-1 original sequence version: 151 DIP protein P35222-1 original sequence version: 171 DIP protein P35222-1 original sequence version: 148 DIP protein P35222 original sequence version: 177 DIP protein P35222 original sequence version: 178 DIP protein P35222-1 original sequence version: 178 CB78F165A3EEF86E pth1r_human Parathyroid hormone/parathyroid hormone-related peptide receptor PTH1R PTHR PTHR1 PTH/PTHrP type I receptor Parathyroid hormone 1 receptor protein protein human Homo sapiens Human MGTARIAPGLALLLCCPVLSSAYALVDADDVMTKEEQIFLLHRAQAQCEKRLKEVLQRPASIMESDKGWTSASTSGKPRKDKASGKLYPESEEDKEAPTGSRYRGRPCLPEWDHILCWPLGAPGEVVAVPCPDYIYDFNHKGHAYRRCDRNGSWELVPGHNRTWANYSECVKFLTNETREREVFDRLGMIYTVGYSVSLASLTVAVLILAYFRRLHCTRNYIHMHLFLSFMLRAVSIFVKDAVLYSGATLDEAERLTEEELRAIAQAPPPPATAAAGYAGCRVAVTFFLYFLATNYYWILVEGLYLHSLIFMAFFSEKKYLWGFTVFGWGLPAVFVAVWVSVRATLANTGCWDLSSGNKKWIIQVPILASIVLNFILFINIVRVLATKLRETNAGRCDTRQQYRKLLKSTLVLMPLFGVHYIVFMATPYTEVSGTLWQVQMHYEMLFNSFQGFFVAIIYCFCNGEVQAEIKKSWSRWTLALDFKRKARSGSSSYSYGPMVSHTSVTNVGPRVGLGLPLSPRLLPTATTNGHPQLPGHAKPGTPALETLETTPPAMAAPKDDGFLNGSCSGLDEEASGPERPPALLQEEWETVM DA1400640A6C7F2B pth1r-ctnnb1 2 9 unspecified role unspecified role bait bait c-terminal fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey physical association physical association no figure pth1r-ctnnb1-1 1 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q03431:p.Trp589Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W A Wed May 02 16:28:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp589ala' Tue Jun 30 18:48:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp589Ala' Q03431:p.Leu585Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A Wed May 02 15:46:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu585ala' Tue Jun 30 18:04:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu585Ala' Q03431:p.Glu587Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E A Wed May 02 20:09:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu587ala' Tue Jun 30 17:45:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu587Ala' Q03431:p.Leu584Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A Wed May 02 17:52:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu584ala' Tue Jun 30 19:30:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu584Ala' physical association physical association 1a ctnnb1-pth1r 4 8 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q03431:p.Leu584Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A Wed May 02 16:28:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu584ala' Tue Jun 30 17:35:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu584Ala' Q03431:p.Leu585Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A Wed May 02 17:14:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu585ala' Tue Jun 30 18:30:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu585Ala' proximity proximity close-contact colocalization neighbourhood interaction 1b ctnnb1-pth1r-1 3 8 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 1c ctnnb1-pth1r-2 5 7 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component colocalization colocalization 1c