UniProt UniProt http://www.uniprot.org Rabankyrin-5 interacts with EHD1 and Vps26 to regulate endocytic trafficking and retromer function. Rabankyrin-5 interacts with EHD1 and Vps26 to regulate endocytic trafficking and retromer function. Traffic (1398-9219) 2012 mimix curation Zhang J., Reiling C., Reinecke J.B., Prislan I., Marky L.A., Sorgen P.L., Naslavsky N., Caplan S. Affinomics - Interactions curated for the Affinomics consortium. Origin of EHD1 (for GST-EH1) according ref. 4 (pmid 12032069 and ref 42 (pmid 15020713). Interactions involving Vps26 not included as the used antibodies might not differentiate between VPS26A and VPS26B. scaplan@unmc.edu 12-Nov-2014: Contacted by IntAct-Help. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Zhang J., Reiling C., Reinecke J.B., Prislan I., Marky L.A., Sorgen P.L., Naslavsky N., Caplan S. Affinomics - Interactions curated for the Affinomics consortium. Origin of EHD1 (for GST-EH1) according ref. 4 (pmid 12032069 and ref 42 (pmid 15020713). Interactions involving Vps26 not included as the used antibodies might not differentiate between VPS26A and VPS26B. scaplan@unmc.edu Accepted 2014-OCT-29 by MGT Traffic (1398-9219) 2012 mimix curation Rabankyrin-5 interacts with EHD1 and Vps26 to regulate endocytic trafficking and retromer function. Rabankyrin-5 interacts with EHD1 and Vps26 to regulate endocytic trafficking and retromer function. Traffic (1398-9219) 2012 mimix curation Zhang J., Reiling C., Reinecke J.B., Prislan I., Marky L.A., Sorgen P.L., Naslavsky N., Caplan S. Affinomics - Interactions curated for the Affinomics consortium. Origin of EHD1 (for GST-EH1) according ref. 4 (pmid 12032069 and ref 42 (pmid 15020713). Interactions involving Vps26 not included as the used antibodies might not differentiate between VPS26A and VPS26B. scaplan@unmc.edu 12-Nov-2014: Contacted by IntAct-Help. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Zhang J., Reiling C., Reinecke J.B., Prislan I., Marky L.A., Sorgen P.L., Naslavsky N., Caplan S. Affinomics - Interactions curated for the Affinomics consortium. Origin of EHD1 (for GST-EH1) according ref. 4 (pmid 12032069 and ref 42 (pmid 15020713). Interactions involving Vps26 not included as the used antibodies might not differentiate between VPS26A and VPS26B. scaplan@unmc.edu Accepted 2014-OCT-29 by MGT Traffic (1398-9219) 2012 mimix curation Rabankyrin-5 interacts with EHD1 and Vps26 to regulate endocytic trafficking and retromer function. Rabankyrin-5 interacts with EHD1 and Vps26 to regulate endocytic trafficking and retromer function. Traffic (1398-9219) 2012 mimix curation Zhang J., Reiling C., Reinecke J.B., Prislan I., Marky L.A., Sorgen P.L., Naslavsky N., Caplan S. Affinomics - Interactions curated for the Affinomics consortium. Origin of EHD1 (for GST-EH1) according ref. 4 (pmid 12032069 and ref 42 (pmid 15020713). Interactions involving Vps26 not included as the used antibodies might not differentiate between VPS26A and VPS26B. scaplan@unmc.edu 12-Nov-2014: Contacted by IntAct-Help. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunostaining immunostaining Zhang J., Reiling C., Reinecke J.B., Prislan I., Marky L.A., Sorgen P.L., Naslavsky N., Caplan S. Affinomics - Interactions curated for the Affinomics consortium. Origin of EHD1 (for GST-EH1) according ref. 4 (pmid 12032069 and ref 42 (pmid 15020713). Interactions involving Vps26 not included as the used antibodies might not differentiate between VPS26A and VPS26B. scaplan@unmc.edu Accepted 2014-OCT-29 by MGT Traffic (1398-9219) 2012 mimix curation Rabankyrin-5 interacts with EHD1 and Vps26 to regulate endocytic trafficking and retromer function. Rabankyrin-5 interacts with EHD1 and Vps26 to regulate endocytic trafficking and retromer function. Traffic (1398-9219) 2012 mimix curation Zhang J., Reiling C., Reinecke J.B., Prislan I., Marky L.A., Sorgen P.L., Naslavsky N., Caplan S. Affinomics - Interactions curated for the Affinomics consortium. Origin of EHD1 (for GST-EH1) according ref. 4 (pmid 12032069 and ref 42 (pmid 15020713). Interactions involving Vps26 not included as the used antibodies might not differentiate between VPS26A and VPS26B. scaplan@unmc.edu 12-Nov-2014: Contacted by IntAct-Help. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Zhang J., Reiling C., Reinecke J.B., Prislan I., Marky L.A., Sorgen P.L., Naslavsky N., Caplan S. Affinomics - Interactions curated for the Affinomics consortium. Origin of EHD1 (for GST-EH1) according ref. 4 (pmid 12032069 and ref 42 (pmid 15020713). Interactions involving Vps26 not included as the used antibodies might not differentiate between VPS26A and VPS26B. scaplan@unmc.edu Accepted 2014-OCT-29 by MGT Traffic (1398-9219) 2012 mimix curation Rabankyrin-5 interacts with EHD1 and Vps26 to regulate endocytic trafficking and retromer function. Rabankyrin-5 interacts with EHD1 and Vps26 to regulate endocytic trafficking and retromer function. Traffic (1398-9219) 2012 mimix curation Zhang J., Reiling C., Reinecke J.B., Prislan I., Marky L.A., Sorgen P.L., Naslavsky N., Caplan S. Affinomics - Interactions curated for the Affinomics consortium. Origin of EHD1 (for GST-EH1) according ref. 4 (pmid 12032069 and ref 42 (pmid 15020713). Interactions involving Vps26 not included as the used antibodies might not differentiate between VPS26A and VPS26B. scaplan@unmc.edu 12-Nov-2014: Contacted by IntAct-Help. in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Zhang J., Reiling C., Reinecke J.B., Prislan I., Marky L.A., Sorgen P.L., Naslavsky N., Caplan S. Affinomics - Interactions curated for the Affinomics consortium. Origin of EHD1 (for GST-EH1) according ref. 4 (pmid 12032069 and ref 42 (pmid 15020713). Interactions involving Vps26 not included as the used antibodies might not differentiate between VPS26A and VPS26B. scaplan@unmc.edu Accepted 2014-OCT-29 by MGT Traffic (1398-9219) 2012 mimix curation Rabankyrin-5 interacts with EHD1 and Vps26 to regulate endocytic trafficking and retromer function. Rabankyrin-5 interacts with EHD1 and Vps26 to regulate endocytic trafficking and retromer function. Traffic (1398-9219) 2012 mimix curation Zhang J., Reiling C., Reinecke J.B., Prislan I., Marky L.A., Sorgen P.L., Naslavsky N., Caplan S. Affinomics - Interactions curated for the Affinomics consortium. Origin of EHD1 (for GST-EH1) according ref. 4 (pmid 12032069 and ref 42 (pmid 15020713). Interactions involving Vps26 not included as the used antibodies might not differentiate between VPS26A and VPS26B. scaplan@unmc.edu 12-Nov-2014: Contacted by IntAct-Help. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Zhang J., Reiling C., Reinecke J.B., Prislan I., Marky L.A., Sorgen P.L., Naslavsky N., Caplan S. Affinomics - Interactions curated for the Affinomics consortium. Origin of EHD1 (for GST-EH1) according ref. 4 (pmid 12032069 and ref 42 (pmid 15020713). Interactions involving Vps26 not included as the used antibodies might not differentiate between VPS26A and VPS26B. scaplan@unmc.edu Accepted 2014-OCT-29 by MGT Traffic (1398-9219) 2012 mimix curation milk1_human MICAL-like protein 1 MICALL1 KIAA1668 MIRAB13 Molecule interacting with Rab13 protein protein human Homo sapiens Human MAGPRGALLAWCRRQCEGYRGVEIRDLSSSFRDGLAFCAILHRHRPDLLDFDSLSKDNVFENNRLAFEVAEKELGIPALLDPNDMVSMSVPDCLSIMTYVSQYYNHFCSPGQAGVSPPRKGLAPCSPPSVAPTPVEPEDVAQGEELSSGSLSEQGTGQTPSSTCAACQQHVHLVQRYLADGRLYHRHCFRCRRCSSTLLPGAYENGPEEGTFVCAEHCARLGPGTRSGTRPGPFSQPKQQHQQQLAEDAKDVPGGGPSSSAPAGAEADGPKASPEARPQIPTKPRVPGKLQELASPPAGRPTPAPRKASESTTPAPPTPRPRSSLQQENLVEQAGSSSLVNGRLHELPVPKPRGTPKPSEGTPAPRKDPPWITLVQAEPKKKPAPLPPSSSPGPPSQDSRQVENGGTEEVAQPSPTASLESKPYNPFEEEEEDKEEEAPAAPSLATSPALGHPESTPKSLHPWYGITPTSSPKTKKRPAPRAPSASPLALHASRLSHSEPPSATPSPALSVESLSSESASQTAGAELLEPPAVPKSSSEPAVHAPGTPGNPVSLSTNSSLASSGELVEPRVEQMPQASPGLAPRTRGSSGPQPAKPCSGATPTPLLLVGDRSPVPSPGSSSPQLQVKSSCKENPFNRKPSPAASPATKKATKGSKPVRPPAPGHGFPLIKRKVQADQYIPEEDIHGEMDTIERRLDALEHRGVLLEEKLRGGLNEGREDDMLVDWFKLIHEKHLLVRRESELIYVFKQQNLEQRQADVEYELRCLLNKPEKDWTEEDRAREKVLMQELVTLIEQRNAIINCLDEDRQREEEEDKMLEAMIKKKEFQREAEPEGKKKGKFKTMKMLKLLGNKRDAKSKSPRDKS 755E3B57C6037292 ehd1_human EH domain-containing protein 1 Testilin CDABP0131 EHD1 PAST PAST1 PAST homolog 1 protein protein human Homo sapiens Human MFSWVSKDARRKKEPELFQTVAEGLRQLYAQKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTGKTTFIRHLIEQDFPGMRIGPEPTTDSFIAVMHGPTEGVVPGNALVVDPRRPFRKLNAFGNAFLNRFMCAQLPNPVLDSISIIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHKLDISDEFSEVIKALKNHEDKIRVVLNKADQIETQQLMRVYGALMWSLGKIINTPEVVRVYIGSFWSHPLLIPDNRKLFEAEEQDLFKDIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPNVFGKESKKKELVNNLGEIYQKIEREHQISPGDFPSLRKMQELLQTQDFSKFQALKPKLLDTVDDMLANDIARLMVMVRQEESLMPSQVVKGGAFDGTMNGPFGHGYGEGAGEGIDDVEWVVGKDKPTYDEIFYTLSPVNGKITGANAKKEMVKSKLPNTVLGKIWKLADVDKDGLLDDEEFALANHLIKVKLEGHELPADLPPHLVPPSKRRHE 3330218772A26CE4 vps35_human Vacuolar protein sorting-associated protein 35 Vesicle protein sorting 35 Maternal-embryonic 3 VPS35 MEM3 TCCCTA00141 protein protein human Homo sapiens Human MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRFIHLLRSEDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL DIP protein Q96QK1 original sequence version: 118 DIP protein Q96QK1 original sequence version: 44 DIP protein Q96QK1 original sequence version: 117 DIP protein Q96QK1 original sequence version: 127 28D2DD1C6B920A0A f1md79_bovin ANKFY1 protein protein bovin Bos taurus (Bovine) Bovine MKEVAKLEKHLMLLRQEYVKLQKKLAETEKRCTLLAAQAKKESSSESFISRLLTIVADLYEQEQYSDLKIKVGSRHINAHKFVLAARSDSWSLASLSSTEELDLSDANPEVTMTMLRWIYTDELEFKEDDVFLTELMKLANRFQLQLLRERCEKGVMSLVNVRNCIRFYQTAEELNASTLMNYCAEIIASHWDDLRKEDFSSMSAQLLYKMIKSKTEYPLHKAIKVEREDVVFLYLIEMDSQLPGKLNELDHNGDLALDLALSRRLESIATTLVNHKADVDMVDKNGWSLLHKGIQRGDLFAATFLIKNGALVNAATLGAQETPLHLVASYNSKKHSAAVMSEMAQLTEALLQAGANPNMQDSKGRTPLHLSIMARNEYVFNQLLQCKQLDLELKDHEGSTALWLAVQYITVSSDQSVNPFEDLPVVNGTSFDENSFAARLIQRGSNTNAPDTVTGNCLLQRAAEAGNEAAALFLATNGAHVNHRNKWGETPLHTACRHGLANLTAELLQQGANPNLQTEEALPSPKEAMSLPSSADSSVYLQTPLHMAIAYNHPDVVSVILEQKANALHATNNLQIIPDFSLKDSRDQTVLGLALWTGMHTIAAQLLGSGACINDTMSDGQTLLHMAMQRQDSKSALFLLEHQADINVRTQDGETALQLAIRNQLPLVVDAICTRGADMSVPDEQGNPPLWLALANNLEDIASTLVRHGCDATCWGPGPSGCLQTLLHRAIDENNEPITCFLIRSGCDVNSPRQPGANGEGEEEARDGQTPLHLAASWGLEETVQCLLEFGANVNAQDAEGRTPVHVAISNQHSVIIQLLISHPDIHLNVRDRQGLTPFACAMTYKNNKAAEAILKRESGAAEQVDNKGRNFLHVAVQNSDIESVLFLISVQANVNSRVQDASKLTPLHLAVQAGSEIIVRNLLLAGAKVNELTKHRQTALHLAAQQDLPTICSVLLENGVDFAAVDENGNNALHLAVMHGRLNNIRVLLTECTVDAEAFNLRGQSPLHILGQYGKENAAAIFDLFLECMPEYPLDKPDAEGNTVLLLAYMKGNANLCRAIVRSGARLGVNNNQGVNIFNYQVATKQLLFRLLDMLSKEPPWCDGSNCYECTAKFGVTTRKHHCRHCGRLLCHKCSTKEIPIIKFDLNKPVRVCNICFDVLTLGGVS 7D605871AF919A91 anfy1_human Rabankyrin-5 Ankyrin repeat and FYVE domain-containing protein 1 ANKFY1 ANKHZN KIAA1255 Ankyrin repeats hooked to a zinc finger motif protein protein human Homo sapiens Human MAEEEVAKLEKHLMLLRQEYVKLQKKLAETEKRCALLAAQANKESSSESFISRLLAIVADLYEQEQYSDLKIKVGDRHISAHKFVLAARSDSWSLANLSSTKELDLSDANPEVTMTMLRWIYTDELEFREDDVFLTELMKLANRFQLQLLRERCEKGVMSLVNVRNCIRFYQTAEELNASTLMNYCAEIIASHWDDLRKEDFSSMSAQLLYKMIKSKTEYPLHKAIKVEREDVVFLYLIEMDSQLPGKLNEADHNGDLALDLALSRRLESIATTLVSHKADVDMVDKSGWSLLHKGIQRGDLFAATFLIKNGAFVNAATLGAQETPLHLVALYSSKKHSADVMSEMAQIAEALLQAGANPNMQDSKGRTPLHVSIMAGNEYVFSQLLQCKQLDLELKDHEGSTALWLAVQHITVSSDQSVNPFEDVPVVNGTSFDENSFAARLIQRGSHTDAPDTATGNCLLQRAAGAGNEAAALFLATNGAHVNHRNKWGETPLHTACRHGLANLTAELLQQGANPNLQTEEALPLPKEAASLTSLADSVHLQTPLHMAIAYNHPDVVSVILEQKANALHATNNLQIIPDFSLKDSRDQTVLGLALWTGMHTIAAQLLGSGAAINDTMSDGQTLLHMAIQRQDSKSALFLLEHQADINVRTQDGETALQLAIRNQLPLVVDAICTRGADMSVPDEKGNPPLWLALANNLEDIASTLVRHGCDATCWGPGPGGCLQTLLHRAIDENNEPTACFLIRSGCDVNSPRQPGANGEGEEEARDGQTPLHLAASWGLEETVQCLLEFGANVNAQDAEGRTPIHVAISSQHGVIIQLLVSHPDIHLNVRDRQGLTPFACAMTFKNNKSAEAILKRESGAAEQVDNKGRNFLHVAVQNSDIESVLFLISVHANVNSRVQDASKLTPLHLAVQAGSEIIVRNLLLAGAKVNELTKHRQTALHLAAQQDLPTICSVLLENGVDFAAVDENGNNALHLAVMHGRLNNIRVLLTECTVDAEAFNLRGQSPLHILGQYGKENAAAIFDLFLECMPGYPLDKPDADGSTVLLLAYMKGNANLCRAIVRSGARLGVNNNQGVNIFNYQVATKQLLFRLLDMLSKEPPWCDGSYCYECTARFGVTTRKHHCRHCGRLLCHKCSTKEIPIIKFDLNKPVRVCNICFDVLTLGGVS DIP protein Q9P2R3 original sequence version: 54 DIP protein Q9P2R3 original sequence version: 132 589297CA4ACDDB56 ehd1-ankfy1 6 8 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association 1A ehd1-ankfy1-1 4 8 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association 1 c ankfy1-ehd1 2 8 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association 2c ankfy1-ehd1-2 3 8 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component colocalization colocalization 2d ankfy1-micall1 2 7 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey association association 4e VPS26A and/or VPS26B detected in the precipitate. ankfy1-ehd1-3 5 8 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component direct interaction direct interaction table 1 ehd1-micall1 1 7 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 5c ankfy1-ehd1-4 4 8 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association 1b