IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Fibroblast growth factor receptor 1- 5-hydroxytryptamine 1A heteroreceptor complexes and their enhancement of hippocampal plasticity. Fibroblast growth factor receptor 1- 5-hydroxytryptamine 1A heteroreceptor complexes and their enhancement of hippocampal plasticity. Biol. Psychiatry (0006-3223) 2012 imex curation Borroto-Escuela D.O., Romero-Fernandez W., Mudó G., Pérez-Alea M., Ciruela F., Tarakanov A.O., Narvaez M., Di Liberto V., Agnati L.F., Belluardo N., Fuxe K. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that the FGFR1 constructs are of HUMAN origin. Kjell.Fuxe@ki.se 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions rat-brain-hippocampus Hippocampus of rat brain rat-brain-hippocampus Hippocampus of rat brain pla proximity ligation assay pELISA pELISA PLA tag visualisation tag visualisation Borroto-Escuela D.O., Romero-Fernandez W., Mudó G., Pérez-Alea M., Ciruela F., Tarakanov A.O., Narvaez M., Di Liberto V., Agnati L.F., Belluardo N., Fuxe K. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that the FGFR1 constructs are of HUMAN origin. Kjell.Fuxe@ki.se Accepted 2013-MAY-28 by PPORRAS 17-Jun-2013: Contacted by IntAct-Help. Biol. Psychiatry (0006-3223) 2012 Only protein-protein interactions imex curation Fibroblast growth factor receptor 1- 5-hydroxytryptamine 1A heteroreceptor complexes and their enhancement of hippocampal plasticity. Fibroblast growth factor receptor 1- 5-hydroxytryptamine 1A heteroreceptor complexes and their enhancement of hippocampal plasticity. Biol. Psychiatry (0006-3223) 2012 imex curation Borroto-Escuela D.O., Romero-Fernandez W., Mudó G., Pérez-Alea M., Ciruela F., Tarakanov A.O., Narvaez M., Di Liberto V., Agnati L.F., Belluardo N., Fuxe K. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that the FGFR1 constructs are of HUMAN origin. Kjell.Fuxe@ki.se 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions rat-brain-hippocampus Hippocampus of rat brain rat-brain-hippocampus Hippocampus of rat brain anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Borroto-Escuela D.O., Romero-Fernandez W., Mudó G., Pérez-Alea M., Ciruela F., Tarakanov A.O., Narvaez M., Di Liberto V., Agnati L.F., Belluardo N., Fuxe K. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that the FGFR1 constructs are of HUMAN origin. Kjell.Fuxe@ki.se Accepted 2013-MAY-28 by PPORRAS 17-Jun-2013: Contacted by IntAct-Help. Biol. Psychiatry (0006-3223) 2012 Only protein-protein interactions imex curation Fibroblast growth factor receptor 1- 5-hydroxytryptamine 1A heteroreceptor complexes and their enhancement of hippocampal plasticity. Fibroblast growth factor receptor 1- 5-hydroxytryptamine 1A heteroreceptor complexes and their enhancement of hippocampal plasticity. Biol. Psychiatry (0006-3223) 2012 imex curation Borroto-Escuela D.O., Romero-Fernandez W., Mudó G., Pérez-Alea M., Ciruela F., Tarakanov A.O., Narvaez M., Di Liberto V., Agnati L.F., Belluardo N., Fuxe K. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that the FGFR1 constructs are of HUMAN origin. Kjell.Fuxe@ki.se 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions rat-neuron Rattus norvegicus neuronal cell neuron Neuron [Neuronal cell; Neural cell confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Borroto-Escuela D.O., Romero-Fernandez W., Mudó G., Pérez-Alea M., Ciruela F., Tarakanov A.O., Narvaez M., Di Liberto V., Agnati L.F., Belluardo N., Fuxe K. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that the FGFR1 constructs are of HUMAN origin. Kjell.Fuxe@ki.se Accepted 2013-MAY-28 by PPORRAS 17-Jun-2013: Contacted by IntAct-Help. Biol. Psychiatry (0006-3223) 2012 Only protein-protein interactions imex curation Fibroblast growth factor receptor 1- 5-hydroxytryptamine 1A heteroreceptor complexes and their enhancement of hippocampal plasticity. Fibroblast growth factor receptor 1- 5-hydroxytryptamine 1A heteroreceptor complexes and their enhancement of hippocampal plasticity. Biol. Psychiatry (0006-3223) 2012 imex curation Borroto-Escuela D.O., Romero-Fernandez W., Mudó G., Pérez-Alea M., Ciruela F., Tarakanov A.O., Narvaez M., Di Liberto V., Agnati L.F., Belluardo N., Fuxe K. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that the FGFR1 constructs are of HUMAN origin. Kjell.Fuxe@ki.se 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. bret bioluminescence resonance energy transfer BRET LRET LRET BRET bret tag visualisation tag visualisation Borroto-Escuela D.O., Romero-Fernandez W., Mudó G., Pérez-Alea M., Ciruela F., Tarakanov A.O., Narvaez M., Di Liberto V., Agnati L.F., Belluardo N., Fuxe K. Affinomics - Interactions curated for the Affinomics consortium. Even after looking at the "high-quality" image for Fig 1D (bottom)- I am not 100% sure which type of blob refers to what construct - so have taken interaction data from the text. (same for Supp fig 3C.) Supp Fig 4C - I just cannot read ANYTHING!....[the mutant HT1A DOES have a GFP tag according to "receptor construct" section but as I cannot read the legend I dont know to enter as a competitor and/or mutation decreasing- so have entered twice- as the M+M "Bret Competition Assay states the mutant was used here (presumably MINUS a GFP tag?) -I am not 100% sure if this is the correct way to enter a mutant that is a competitor... The authors confirm that the FGFR1 constructs are of HUMAN origin. Kjell.Fuxe@ki.se Accepted 2013-MAY-28 by PPORRAS 17-Jun-2013: Contacted by IntAct-Help. Biol. Psychiatry (0006-3223) 2012 Only protein-protein interactions imex curation Fibroblast growth factor receptor 1- 5-hydroxytryptamine 1A heteroreceptor complexes and their enhancement of hippocampal plasticity. Fibroblast growth factor receptor 1- 5-hydroxytryptamine 1A heteroreceptor complexes and their enhancement of hippocampal plasticity. Biol. Psychiatry (0006-3223) 2012 imex curation Borroto-Escuela D.O., Romero-Fernandez W., Mudó G., Pérez-Alea M., Ciruela F., Tarakanov A.O., Narvaez M., Di Liberto V., Agnati L.F., Belluardo N., Fuxe K. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that the FGFR1 constructs are of HUMAN origin. Kjell.Fuxe@ki.se 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. pla proximity ligation assay pELISA pELISA PLA tag visualisation tag visualisation Borroto-Escuela D.O., Romero-Fernandez W., Mudó G., Pérez-Alea M., Ciruela F., Tarakanov A.O., Narvaez M., Di Liberto V., Agnati L.F., Belluardo N., Fuxe K. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that the FGFR1 constructs are of HUMAN origin. Kjell.Fuxe@ki.se Accepted 2013-MAY-28 by PPORRAS 17-Jun-2013: Contacted by IntAct-Help. Biol. Psychiatry (0006-3223) 2012 Only protein-protein interactions imex curation Fibroblast growth factor receptor 1- 5-hydroxytryptamine 1A heteroreceptor complexes and their enhancement of hippocampal plasticity. Fibroblast growth factor receptor 1- 5-hydroxytryptamine 1A heteroreceptor complexes and their enhancement of hippocampal plasticity. Biol. Psychiatry (0006-3223) 2012 imex curation Borroto-Escuela D.O., Romero-Fernandez W., Mudó G., Pérez-Alea M., Ciruela F., Tarakanov A.O., Narvaez M., Di Liberto V., Agnati L.F., Belluardo N., Fuxe K. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that the FGFR1 constructs are of HUMAN origin. Kjell.Fuxe@ki.se 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Borroto-Escuela D.O., Romero-Fernandez W., Mudó G., Pérez-Alea M., Ciruela F., Tarakanov A.O., Narvaez M., Di Liberto V., Agnati L.F., Belluardo N., Fuxe K. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that the FGFR1 constructs are of HUMAN origin. Kjell.Fuxe@ki.se Accepted 2013-MAY-28 by PPORRAS 17-Jun-2013: Contacted by IntAct-Help. Biol. Psychiatry (0006-3223) 2012 Only protein-protein interactions imex curation 5ht1a_human 5-hydroxytryptamine receptor 1A HTR1A ADRB2RL1 ADRBRL1 G-21 Serotonin receptor 1A protein protein human Homo sapiens Human MDVLSPGQGNNTTSPPAPFETGGNTTGISDVTVSYQVITSLLLGTLIFCAVLGNACVVAAIALERSLQNVANYLIGSLAVTDLMVSVLVLPMAALYQVLNKWTLGQVTCDLFIALDVLCCTSSILHLCAIALDRYWAITDPIDYVNKRTPRRAAALISLTWLIGFLISIPPMLGWRTPEDRSDPDACTISKDHGYTIYSTFGAFYIPLLLMLVLYGRIFRAARFRIRKTVKKVEKTGADTRHGASPAPQPKKSVNGESGSRNWRLGVESKAGGALCANGAVRQGDDGAALEVIEVHRVGNSKEHLPLPSEAGPTPCAPASFERKNERNAEAKRKMALARERKTVKTLGIIMGTFILCWLPFFIVALVLPFCESSCHMPTLLGAIINWLGYSNSLLNPVIYAYFNKDFQNAFKKIIKCKFCRQ 762664FCF62CFD8F 5ht1a_rat 5-hydroxytryptamine receptor 1A Htr1a 5ht1a Serotonin receptor 1A protein protein rat Rattus norvegicus (Rat) Rat MDVFSFGQGNNTTASQEPFGTGGNVTSISDVTFSYQVITSLLLGTLIFCAVLGNACVVAAIALERSLQNVANYLIGSLAVTDLMVSVLVLPMAALYQVLNKWTLGQVTCDLFIALDVLCCTSSILHLCAIALDRYWAITDPIDYVNKRTPRRAAALISLTWLIGFLISIPPMLGWRTPEDRSDPDACTISKDHGYTIYSTFGAFYIPLLLMLVLYGRIFRAARFRIRKTVRKVEKKGAGTSLGTSSAPPPKKSLNGQPGSGDWRRCAENRAVGTPCTNGAVRQGDDEATLEVIEVHRVGNSKEHLPLPSESGSNSYAPACLERKNERNAEAKRKMALARERKTVKTLGIIMGTFILCWLPFFIVALVLPFCESSCHMPALLGAIINWLGYSNSLLNPVIYAYFNKDFQNAFKKIIKCKFCRR 19C2731834C4BBC9 fgfr1_rat Fibroblast growth factor receptor 1 Fgfr1 Flg MFR Proto-oncogene c-Fgr Basic fibroblast growth factor receptor 1 protein protein rat Rattus norvegicus (Rat) Rat MWGWRGLLFWAVLVTATLCTARPAPTLPEQAQPWGVPVEVESLLVHPGDLLQLRCRLRDDVQSINWLRDGVQLAESNRTRITGEEVEVRDSIPADSGLYACVTNSPSGSDTTYFSVNVSDALPSSEDDDDDDDSSSEEKETDNTKPNRRPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPSPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPYDQILKTAGVNTTDKEMEVLHLRNVSFEDAGEYTCLAGNSIGLSHHSAWLTVLEALEERPAVMTSPLYLEIIIYCTGAFLISCMVGSVIIYKMKSGTKKSDFHSQMAVHKLAKSIPLRRQVTVSADSSASMNSGVLLVRPSRLSSSGTPMLAGVSEYELPEDPRWELPRDRLVLGKPLGEGCFGQVVLAEAIGLDKDKPNRVTKVAVKMLKSDATEKDLSDLISEMEMMKMIGKHKNIINLLGACTQDGPLYVIVEYASKGNLREYLQARRPPGLEYCYNPSHNPEEQLSSKDLVSCAYQVARGMEYLASKKCIHRDLAARNVLVTEDNVMKIADFGLARDIHHIDYYKKTTNGRLPVKWMAPEALFDRIYTHQSDVWSFGVLLWEIFTLGGSPNPGVPVEELFKLLKEGHRMDKPSNCTNELYMMMRDCWNAVPSQRPTFKQLVEDLDRIVALTSNQEYLDLSMPLDQDSPSFPDTRSSTCSSGEDSVFSHEPFPEEPCLPRHPTQLANGGLNRR E59D924D0A1DE5C5 5ht1b_human 5-hydroxytryptamine receptor 1B HTR1B HTR1DB Serotonin receptor 1B Serotonin 1D beta receptor S12 protein protein human Homo sapiens Human MEEPGAQCAPPPPAGSETWVPQANLSSAPSQNCSAKDYIYQDSISLPWKVLLVMLLALITLATTLSNAFVIATVYRTRKLHTPANYLIASLAVTDLLVSILVMPISTMYTVTGRWTLGQVVCDFWLSSDITCCTASILHLCVIALDRYWAITDAVEYSAKRTPKRAAVMIALVWVFSISISLPPFFWRQAKAEEEVSECVVNTDHILYTVYSTVGAFYFPTLLLIALYGRIYVEARSRILKQTPNRTGKRLTRAQLITDSPGSTSSVTSINSRVPDVPSESGSPVYVNQVKVRVSDALLEKKKLMAARERKATKTLGIILGAFIVCWLPFFIISLVMPICKDACWFHLAIFDFFTWLGYLNSLINPIIYTMSNEDFKQAFHKLIRFKCTS CD874DC7EB44CF12 fgfr1_human Fibroblast growth factor receptor 1 FGFR1 FGFBR FLG FLT2 Fms-like tyrosine kinase 2 Proto-oncogene c-Fgr BFGFR CEK HBGFR Basic fibroblast growth factor receptor 1 N-sam protein protein human Homo sapiens Human MWSWKCLLFWAVLVTATLCTARPSPTLPEQAQPWGAPVEVESFLVHPGDLLQLRCRLRDDVQSINWLRDGVQLAESNRTRITGEEVEVQDSVPADSGLYACVTSSPSGSDTTYFSVNVSDALPSSEDDDDDDDSSSEEKETDNTKPNRMPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPYVQILKTAGVNTTDKEMEVLHLRNVSFEDAGEYTCLAGNSIGLSHHSAWLTVLEALEERPAVMTSPLYLEIIIYCTGAFLISCMVGSVIVYKMKSGTKKSDFHSQMAVHKLAKSIPLRRQVTVSADSSASMNSGVLLVRPSRLSSSGTPMLAGVSEYELPEDPRWELPRDRLVLGKPLGEGCFGQVVLAEAIGLDKDKPNRVTKVAVKMLKSDATEKDLSDLISEMEMMKMIGKHKNIINLLGACTQDGPLYVIVEYASKGNLREYLQARRPPGLEYCYNPSHNPEEQLSSKDLVSCAYQVARGMEYLASKKCIHRDLAARNVLVTEDNVMKIADFGLARDIHHIDYYKKTTNGRLPVKWMAPEALFDRIYTHQSDVWSFGVLLWEIFTLGGSPYPGVPVEELFKLLKEGHRMDKPSNCTNELYMMMRDCWHAVPSQRPTFKQLVEDLDRIVALTSNQEYLDLSMPLDQYSPSFPDTRSSTCSSGEDSVFSHEPLPEEPCLPRHPAQLANGGLKRR DIP protein P11362 original sequence version: 154 DIP protein P11362 original sequence version: 160 DIP protein P11362 original sequence version: 180 DIP protein P11362 original sequence version: 170 93A01B5D78C3E72C fgfr1-htr1a-24 2 8 unspecified role unspecified role unspecified role unspecified role 9 unspecified role unspecified role unspecified role unspecified role physical association physical association 1B fgfr1-htr1a-2 1 8 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 1A,1C, Supp1C 8-OH-DPAT ((±)-7-Hydroxy 2-dipropylaminotetralin hydrobromide), -a 5-HT1A receptor agonist +FGF2 fgfr1-htr1a-4 4 11 unspecified role unspecified role fluorescence donor fluorescence donor c_terminus renilla luciferase renilla luciferase protein tag renilla luciferase c-term range C-terminal range c-term range c-term range C-terminal range c-term range 7 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P08908:p.[Asp406_Asn409delinsAlaAlaAlaAla;Phe411_Lys416delinsAlaAlaAlaAlaAlaAla] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain FKKIIK AAAAAA certain certain sequence position certain certain certain sequence position certain DFQN AAAA Fri Jul 22 10:50:12 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'asp406ala,phe407ala,gln408ala,ans409ala,phe411ala,lys412ala,lys413ala,ile414ala,ile415ala,lys416ala' Wed May 02 19:22:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp_phe_gln_asn406-409ala_ala_ala_ala,phe_lys_lys_ile_ile_lys411-416ala_ala_ala_ala_ala_ala' Tue Jun 30 19:25:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp406_Asn409delinsAlaAlaAlaAla;Phe411_Lys416delinsAlaAlaAlaAlaAlaAla]' proximity proximity close-contact colocalization neighbourhood interaction 1D, 1E, supp 3B, supp 3C, supp 4C. 8-OH-DPAT ((±)-7-Hydroxy 2-dipropylaminotetralin hydrobromide), -a 5-HT1A receptor agonist +FGF2 fgfr1-fgfr1 4 7 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P08908:p.[Asp406_Asn409delinsAlaAlaAlaAla;Phe411_Lys416delinsAlaAlaAlaAlaAlaAla] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain FKKIIK AAAAAA certain certain sequence position certain certain certain sequence position certain DFQN AAAA Fri Jul 22 10:58:28 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'asp406ala,phe407ala,gln408ala,ans409ala,phe411ala,lys412ala,lys413ala,ile414ala,ile415ala,lys416ala' Wed May 02 20:15:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp_phe_gln_asn406-409ala_ala_ala_ala,phe_lys_lys_ile_ile_lys411-416ala_ala_ala_ala_ala_ala' Tue Jun 30 19:43:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp406_Asn409delinsAlaAlaAlaAla;Phe411_Lys416delinsAlaAlaAlaAlaAlaAla]' 11 unspecified role unspecified role fluorescence donor fluorescence donor c_terminus renilla luciferase renilla luciferase protein tag renilla luciferase c-term range C-terminal range c-term range c-term range C-terminal range c-term range 11 competitor competitor competitor neutral component neutral component 7 competitor competitor competitor neutral component neutral component h8-mutant_asp406ala,phe407ala,gln408ala,ans409ala,phe411ala,lys412ala,lys413ala,ile414ala,ile415ala,lys416ala sufficient to bind sufficient binding region sufficient to bind alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 competitor competitor competitor neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 1D, 1E, supp 3B, supp 3C, supp 4C 8-OH-DPAT ((±)-7-Hydroxy 2-dipropylaminotetralin hydrobromide), -a 5-HT1A receptor agonist +FGF2 Treatment with FGF2 or 8-OH-DPAT significantly reduced the BRET50 values and thus increased the affinity of the FGFR1–5-HT1A heteroreceptor interaction (Figure 1D, also see Fig Supp 3C). The 5-HT1A-Hx8 mutant showed an increased BRET50 value versus the wild-type but has a reduced ability to form a receptor complex with FGFR1. fgfr1-htr1a-8 5 11 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction Supp 1B 8-OH-DPAT ((±)-7-Hydroxy 2-dipropylaminotetralin hydrobromide), -a 5-HT1A receptor agonist +FGF2 fgfr1-htr1a-10 6 11 unspecified role unspecified role neutral component neutral component c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 7 unspecified role unspecified role neutral component neutral component c_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association supp 2B 8-OH-DPAT ((±)-7-Hydroxy 2-dipropylaminotetralin hydrobromide), -a 5-HT1A receptor agonist +FGF2 fgfr1-htr1a-11 3 7 unspecified role unspecified role neutral component neutral component c_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 11 unspecified role unspecified role neutral component neutral component c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association supp 2A fgfr1-fgfr1-1 4 7 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 11 unspecified role unspecified role fluorescence donor fluorescence donor c_terminus renilla luciferase renilla luciferase protein tag renilla luciferase c-term range C-terminal range c-term range c-term range C-terminal range c-term range 7 competitor competitor competitor neutral component neutral component trans_membrane_region_5 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trans_membrane_region_7 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain proximity proximity close-contact colocalization neighbourhood interaction 1E