IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Spatio-temporal composition of the mitotic Chromosomal Passenger Complex detected using in situ proximity ligation assay. Spatio-temporal composition of the mitotic Chromosomal Passenger Complex detected using in situ proximity ligation assay. Mol Oncol (1574-7891) 2011 imex curation Vuoriluoto M., Laine L.J., Saviranta P., Pouwels J., Kallio M.J. Affinomics - Interactions curated for the Affinomics consortium. marko.kallio@vtt.fi 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Vuoriluoto M., Laine L.J., Saviranta P., Pouwels J., Kallio M.J. Affinomics - Interactions curated for the Affinomics consortium. marko.kallio@vtt.fi Accepted 2013-SEP-03 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. Mol Oncol (1574-7891) 2011 Only protein-protein interactions imex curation Spatio-temporal composition of the mitotic Chromosomal Passenger Complex detected using in situ proximity ligation assay. Spatio-temporal composition of the mitotic Chromosomal Passenger Complex detected using in situ proximity ligation assay. Mol Oncol (1574-7891) 2011 imex curation Vuoriluoto M., Laine L.J., Saviranta P., Pouwels J., Kallio M.J. Affinomics - Interactions curated for the Affinomics consortium. marko.kallio@vtt.fi 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Vuoriluoto M., Laine L.J., Saviranta P., Pouwels J., Kallio M.J. Affinomics - Interactions curated for the Affinomics consortium. marko.kallio@vtt.fi Accepted 2013-SEP-03 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. Mol Oncol (1574-7891) 2011 Only protein-protein interactions imex curation birc5_human Baculoviral IAP repeat-containing protein 5 Apoptosis inhibitor survivin Apoptosis inhibitor 4 BIRC5 API4 IAP4 protein protein human Homo sapiens Human MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTENEPDLAQCFFCFKELEGWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGEFLKLDRERAKNKIAKETNNKKKEFEETAKKVRRAIEQLAAMD DIP protein O15392 original sequence version: 129 DIP protein O15392 original sequence version: 132 DIP protein O15392-1 original sequence version: 141 DIP protein O15392-1 original sequence version: 143 9E7CADCDF2822286 borea_human Borealin Dasra-B Cell division cycle-associated protein 8 Pluripotent embryonic stem cell-related gene 3 protein CDCA8 PESCRG3 protein protein human Homo sapiens Human MAPRKGSSRVAKTNSLRRRKLASFLKDFDREVEIRIKQIESDRQNLLKEVDNLYNIEILRLPKALREMNWLDYFALGGNKQALEEAATADLDITEINKLTAEAIQTPLKSAKTRKVIQVDEMIVEEEEEEENERKNLQTARVKRCPPSKKRTQSIQGKGKGKRSSRANTVTPAVGRLEVSMVKPTPGLTPRFDSRVFKTPGLRTPAAGERIYNISGNGSPLADSKEIFLTVPVGGGESLRLLASDLQRHSIAQLDPEALGNIKKLSNRLAQICSSIRTHK DIP protein Q53HL2 original sequence version: 57 519978A7C295C571 plk1_human Serine/threonine-protein kinase PLK1 Polo-like kinase 1 Serine/threonine-protein kinase 13 PLK1 PLK protein protein human Homo sapiens Human MSAAVTAGKLARAPADPGKAGVPGVAAPGAPAAAPPAKEIPEVLVDPRSRRRYVRGRFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPHQREKMSMEISIHRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLELHKRRKALTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTINELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGLENPLPERPREKEEPVVRETGEVVDCHLSDMLQQLHSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLKYFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGSVQINFFQDHTKLILCPLMAAVTYIDEKRDFRTYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSASNRLKAS DIP protein P53350 original sequence version: 169 DIP protein P53350 original sequence version: 170 DIP protein P53350 original sequence version: 164 DIP protein P53350 original sequence version: 154 DIP protein P53350 original sequence version: 91 DIP protein P53350 original sequence version: 145 DIP protein P53350 original sequence version: 121 DIP protein P53350 original sequence version: 118 DIP protein P53350 original sequence version: 123 DIP protein P53350 original sequence version: 119 DIP protein P53350 original sequence version: 138 DIP protein P53350 original sequence version: 163 DIP protein P53350 original sequence version: 178 178C2F13C10E8206 aurkb_human Aurora kinase B AIRK2 Aurora- and IPL1-like midbody-associated protein 1 Aurora/IPL1-related kinase 2 STK-1 Serine/threonine-protein kinase 12 Serine/threonine-protein kinase 5 STK1 STK5 Aurora 1 AURKB AIK2 AIM1 ARK2 STK12 Serine/threonine-protein kinase aurora-B protein protein human Homo sapiens Human MAQKENSYPWPYGRQTAPSGLSTLPQRVLRKEPVTPSALVLMSRSNVQPTAAPGQKVMENSSGTPDILTRHFTIDDFEIGRPLGKGKFGNVYLAREKKSHFIVALKVLFKSQIEKEGVEHQLRREIEIQAHLHHPNILRLYNYFYDRRRIYLILEYAPRGELYKELQKSCTFDEQRTATIMEELADALMYCHGKKVIHRDIKPENLLLGLKGELKIADFGWSVHAPSLRRKTMCGTLDYLPPEMIEGRMHNEKVDLWCIGVLCYELLVGNPPFESASHNETYRRIVKVDLKFPASVPMGAQDLISKLLRHNPSERLPLAQVSAHPWVRANSRRVLPPSALQSVA DIP protein Q96GD4 original sequence version: 88 DIP protein Q96GD4 original sequence version: 113 DIP protein Q96GD4 original sequence version: 116 DIP protein Q96GD4 original sequence version: 122 DIP protein Q96GD4 original sequence version: 177 DIP protein Q96GD4-1 original sequence version: 177 A5ED13EF5A1FAFBF ince_human Inner centromere protein INCENP protein protein human Homo sapiens Human MGTTAPGPIHLLELCDQKLMEFLCNMDNKDLVWLEEIQEEAERMFTREFSKEPELMPKTPSQKNRRKKRRISYVQDENRDPIRRRLSRRKSRSSQLSSRRLRSKDSVEKLATVVGENGSVLRRVTRAAAAAAAATMALAAPSSPTPESPTMLTKKPEDNHTQCQLVPVVEIGISERQNAEQHVTQLMSTEPLPRTLSPTPASATAPTSQGIPTSDEESTPKKSKARILESITVSSLMATPQDPKGQGVGTGRSASKLRIAQVSPGPRDSPAFPDSPWRERVLAPILPDNFSTPTGSRTDSQSVRHSPIAPSSPSPQVLAQKYSLVAKQESVVRRASRRLAKKTAEEPAASGRIICHSYLERLLNVEVPQKVGSEQKEPPEEAEPVAAAEPEVPENNGNNSWPHNDTEIANSTPNPKPAASSPETPSAGQQEAKTDQADGPREPPQSARRKRSYKQAVSELDEEQHLEDEELQPPRSKTPSSPCPASKVVRPLRTFLHTVQRNQMLMTPTSAPRSVMKSFIKRNTPLRMDPKCSFVEKERQRLENLRRKEEAEQLRRQKVEEDKRRRLEEVKLKREERLRKVLQARERVEQMKEEKKKQIEQKFAQIDEKTEKAKEERLAEEKAKKKAAAKKMEEVEARRKQEEEARRLRWLQQEEEERRHQELLQKKKEEEQERLRKAAEAKRLAEQREQERREQERREQERREQERREQERREQERQLAEQERRREQERLQAERELQEREKALRLQKEQLQRELEEKKKKEEQQRLAERQLQEEQEKKAKEAAGASKALNVTVDVQSPACTSYQMTPQGHRAPPKINPDNYGMDLNSDDSTDDEAHPRKPIPTWARGTPLSQAIIHQYYHPPNLLELFGTILPLDLEDIFKKSKPRYHKRTSSAVWNSPPLQGARVPSSLAYSLKKH DIP protein Q9NQS7 original sequence version: 83 DIP protein Q9NQS7 original sequence version: 86 644D081DCC9035E8 birc5-cdca8-14 1 4 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component colocalization colocalization 2a Interaction detected during the metaphase. birc5-cdca8-16 2 7 ancillary ancillary neutral component neutral component 4 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 2b, 2d, 2e, 3c ZM447439 (aurora B kinase inhibitor), upon MG132 treatment (required to stop cell cycle and make the effect detectable). Interaction found during pro-metaphase and metaphase. aurkb-birc5-17 2 3 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component 7 ancillary ancillary neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 2d, 2e, 3c ZM447439 (aurora B kinase inhibitor), upon MG132 treatment (required to stop cell cycle and make the effect detectable). Interaction found during pro-metaphase and metaphase. aurkb-borea-18 2 6 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component 7 ancillary ancillary neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 2d, 2e, 3c ZM447439 (aurora B kinase inhibitor), upon MG132 treatment (required to stop cell cycle and make the effect detectable). Interaction found during pro-metaphase and metaphase. aurkb-cdca8-2 2 7 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 2d, 2e, 3c ZM447439 (aurora B kinase inhibitor), upon MG132 treatment (required to stop cell cycle and make the effect detectable). Interaction found during pro-metaphase and metaphase. birc5-cdca8-17 2 3 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 2b, 2d, 2e, 3a, 3b, 3c ZM447439 (aurora B kinase inhibitor), upon MG132 treatment (required to stop cell cycle and make the effect detectable). Interaction found during pro-metaphase, metaphase, anaphase and telophase. Interaction detected in the kinetochore pre-anaphase and in the spindle midzone post-anaphase. incenp-plk1 2 5 unspecified role unspecified role neutral component neutral component 7 ancillary ancillary neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 4a Interaction found during all cell-cycle phases. The interaction greatly increases during anaphase, when it is located in the central spindle, and even more during telophase, when it locates to the midbody.