mpidb The Microbial Protein Interaction Database zielenkiewi-2009-1 In vivo interactions between toxin-antitoxin proteins epsilon and zeta of streptococcal plasmid pSM19035 in Saccharomyces cerevisiae. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Zielenkiewicz U., Kowalewska M., Kaczor C., Ceglowski P. ulazet@ibb.waw.pl J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation ztox_strpy Toxin zeta UDP-N-acetylglucosamine kinase protein protein strpy Streptococcus pyogenes MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYETMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI eatx_strpy Antitoxin epsilon protein protein strpy Streptococcus pyogenes MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA ztox-eatx 1335723 not-defined-491390883 1335724 unspecified role unspecified role bait bait not-defined-104542570 1335725 unspecified role unspecified role prey prey region Q57231-1-12 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false table 3, 4 eatx-ztox 1335723 not-defined-104542570 1335725 unspecified role unspecified role bait bait not-defined-491390883 1335724 unspecified role unspecified role prey prey physical association physical association false false false table 3 ztox-eatx-1 1335723 not-defined-1733962541 1335725 unspecified role unspecified role prey prey region Q57231-1-66 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false region Q57231-1-29 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false not-defined-867017765 1335724 unspecified role unspecified role bait bait physical association physical association false false false table 3, 4 DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ mutschler-2011-1 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Mutschler H., Gebhardt M., Shoeman RL., Meinhart A. DIP original experiment: DIP-216767X PLoS biology 2011 IMEx A novel mechanism of programmed cell death in bacteria by toxin-antitoxin systems corrupts peptidoglycan synthesis. Mutschler H., Gebhardt M., Shoeman RL., Meinhart A. Only protein-protein interactions imex curation dong-2017-6 in vitro In vitro pull down pull down experimental particp experimental participant identification experimental particp Dong null., Guo M., Wang S., Zhu Y., Wang S., Xiong Z., Yang J., Xu Z., Huang Z. huangzhiwei@hit.edu.cn Nature 2017 imex curation Only protein-protein interactions imex curation dong-2017-5 in vitro In vitro mst microscale thermophoresis mst experimental particp experimental participant identification experimental particp Dong null., Guo M., Wang S., Zhu Y., Wang S., Xiong Z., Yang J., Xu Z., Huang Z. huangzhiwei@hit.edu.cn Nature 2017 imex curation Only protein-protein interactions imex curation dong-2017-3 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column experimental particp experimental participant identification experimental particp Dong null., Guo M., Wang S., Zhu Y., Wang S., Xiong Z., Yang J., Xu Z., Huang Z. huangzhiwei@hit.edu.cn Nature 2017 imex curation Only protein-protein interactions imex curation dong-2017-4 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction experimental particp experimental participant identification experimental particp Dong null., Guo M., Wang S., Zhu Y., Wang S., Xiong Z., Yang J., Xu Z., Huang Z. huangzhiwei@hit.edu.cn Nature 2017 imex curation Only protein-protein interactions imex curation smith-2011-5 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Smith CL., Ghosh J., Elam JS., Pinkner JS., Hultgren SJ., Caparon MG., Ellenberger T. DIP original experiment: DIP-218758X Structure (London, England : 1993) 2011 IMEx Structural basis of Streptococcus pyogenes immunity to its NAD+ glycohydrolase toxin. Smith CL., Ghosh J., Elam JS., Pinkner JS., Hultgren SJ., Caparon MG., Ellenberger T. Only protein-protein interactions imex curation smith-2011-6 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Smith CL., Ghosh J., Elam JS., Pinkner JS., Hultgren SJ., Caparon MG., Ellenberger T. DIP original experiment: DIP-218756X Structure (London, England : 1993) 2011 IMEx Structural basis of Streptococcus pyogenes immunity to its NAD+ glycohydrolase toxin. Smith CL., Ghosh J., Elam JS., Pinkner JS., Hultgren SJ., Caparon MG., Ellenberger T. Only protein-protein interactions imex curation smith-2011-7 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Smith CL., Ghosh J., Elam JS., Pinkner JS., Hultgren SJ., Caparon MG., Ellenberger T. DIP original experiment: DIP-218759X Structure (London, England : 1993) 2011 IMEx Structural basis of Streptococcus pyogenes immunity to its NAD+ glycohydrolase toxin. Smith CL., Ghosh J., Elam JS., Pinkner JS., Hultgren SJ., Caparon MG., Ellenberger T. Only protein-protein interactions imex curation smith-2011-8 ecolx Escherichia coli ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Smith CL., Ghosh J., Elam JS., Pinkner JS., Hultgren SJ., Caparon MG., Ellenberger T. DIP original experiment: DIP-218757X Structure (London, England : 1993) 2011 IMEx Structural basis of Streptococcus pyogenes immunity to its NAD+ glycohydrolase toxin. Smith CL., Ghosh J., Elam JS., Pinkner JS., Hultgren SJ., Caparon MG., Ellenberger T. Only protein-protein interactions imex curation schwarz_linek-2003-2 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Schwarz-Linek U., Werner JM., Pickford AR., Gurusiddappa S., Kim JH., Pilka ES., Briggs JA., Gough TS., Höök M., Campbell ID., Potts JR. jennifer.potts@bioch.ox.ac.uk Nature 2003 imex curation Only protein-protein interactions imex curation q2vj58_strpy E0F66_06230 ifs GTK54_00720 SPNIH34_01310 protein protein strpy Streptococcus pyogenes MYKVPKGLEHYQKMFQKEVTVNDLKKYLIGSDKEYRITRRDSYMGDISDPEVILEYGVYPAFIKGYTQLKANIEEALLEMSNSGQALDIYQAVQTLNAENMLLNYYESLPFYLNRQSILANITKALKDAHIREAMAHYKLGEFAHYQDTMLDMVERTIETF BEB604A6FB730D7B q5xe42_strp6 M6_Spy0186 protein protein strp6 Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394) MVYMRNKKVTLAHIVAKTSVAIALAGAMGSSLLANSTTYAVSGKENKKSDVKYETTKVMEANATSSKEDNHVMHTLDGSMSTVWEENSPGGGVGEVLSYKFASPMRIGRILIVNGDTSSKENYYKKNRIAKADVKYYNGNKLVLFQKIELGDTYTKKPHHIEIDKKLDVDRIDIEVTEVHQGQNKDILALSEVTFGNMERDLFEKKFKEIKDKWVTDKQADEFIETADKYADKAVQMSAVASRAEYYRMYVSRKYQYKKEFVEKLKQVYKESGASHVTSKKDLMLAFDDAKRKSTIGRQENGLFVTSFAEDMALLFTDQGKLKSADQIENIKGVDSGKYSDGVYQYEYDSELTKNIDKLGYIRTASGDTPGANSLNIPGCQTWSGKHIENSESELIFPSISVKDLKSKAVLAEIDAKGYFEIIDPTIIAPNGDHKKVTGRFKIKKMQDRMQDRK D9FBF97B708F7828 ztox_strpy Toxin zeta UDP-N-acetylglucosamine kinase protein protein strpy Streptococcus pyogenes MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYETMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI eatx_strpy Antitoxin epsilon protein protein strpy Streptococcus pyogenes MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA finc_human Fibronectin FN1 FN Cold-insoluble globulin protein protein human Homo sapiens Human MLRGPGPGLLLLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPVAVSQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEAEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQTTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSVGYYTVTGLEPGIDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSGESQPLVQTAVTNIDRPKGLAFTDVDVDSIKIAWESPQGQVSRYRVTYSSPEDGIHELFPAPDGEEDTAELQGLRPGSEYTVSVVALHDDMESQPLIGTQSTAIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEILDVPSTVQKTPFVTHPGYDTGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPIRHRPRPYPPNVGEEIQIGHIPREDVDYHLYPHGPGLNPNASTGQEALSQTTISWAPFQDTSEYIISCHPVGTDEEPLQFRVPGTSTSATLTGLTRGATYNVIVEALKDQQRHKVREEVVTVGNSVNEGLNQPTDDSCFDPYTVSHYAVGDEWERMSESGFKLLCQCLGFGSGHFRCDSSRWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGGEPSPEGTTGQSYNQYSQRYHQRTNTNVNCPIECFMPLDVQADREDSRE q53971_strdy fnbB protein protein Streptococcus dysgalactiae Streptococcus dysgalactiae MKNKVLKTLVLFLFAVLGIFSIQTVRAAGKSVNLQVKAQTEQINHGDSVRFEVNFGGPGTKVEKNQTVNISFIGDYAKINLPKESLILKDSNDDAILGRVEFSDNKAILIFNEEASHRDDIVGGFFFDARGIYNGDVKSSGKGSIRVEYENKYRIININYNAGGSRTDSVYAKSGVLDDFDSTGRIVNWYFNFNAALNPIKDEDVEFMTTDNLPQTMTWDIEAVKKDPYVVQIRGGYLGTVFSKNGGWIDIEEATKLGIEIIFSGQKLTIKIPKWTNYNGLIPYVKPLNQTSISVKLTAKITEKTMRDQTIEYVENDSIPKIEGIEWNLDKNQFGAKVRIQRSGSWILGVRPGDLKVLKFERDKSKAIPKVKFRLIREDNHEIEAKMGKESYQNLGRFYDFETGEDGSVTLKGLKDGIYLLKEIGNNLVDLSKPIVWDNLSLREKIELNQEQPYKVIISSTQQPNGIVLKVENELKSEKVDIKVTKKWEDYQDKFKKRPDHILLDLEANGEKVEGHTSKIVTKGMDDKWSYTFKDLPKYNDRGEKISYKVVEVQVNEYETPIIEEISPYNFTITNKYINKETTELSGKKIWENDRANQRPEQIQVQLLQNGQKMPNKIQEIKANDWTYKFKDLPKYDETGKEYKYSVEEVTVPDGYKVSYLGNDIYNTRETEFIFENNNFSLEFGNSEVKGQSGSKVITEDTLTSFKGRKLWKNDAATDRPEAIQVQLYADGVAVEGQTKYVSGSGDEWSFEFKNLKKHGESGKEIVYSVKEVTVPAGYDVTYSDNDIINTKREVIIQQGPILGLEETLPTEEHQSGDTTTIEDTRPIDTMSGLSGETGQSGNTTIEEDSTTHVKFSKRDINGKELAGAMIELRNLSGQTIQSWISDGTVKVFYLMPGTYQFVETAAPEGYELAAPITFTIDEKGQIWVDSTLIVGDDPIVMIDNDKPITEASQSIDFEETLPTEQGQSGSTTEVEDTKGPEVIIGGQGEIVDIEENLPTEQGQSGSTTEVEDTKGPEVIIGGQGEVVDIEESLPTEQGQSGSTTEVEDSKPKLSIHFDNEWPKEDKPQLPAVEKPKTKESLPAAGEAEHVLSTIVGAMILFLVSLWGLLKRKASKA fnba_staa8 Fibronectin-binding protein A fnbA SAOUHSC_02803 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTTVEENGNSATDNKTSETQTTATNVNHIEETQSYNATVTEQPSNATQVTTEEAPKAVQAPQTAQPANIETVKEEVVKEEAKPQVKETTQSQDNSGDQRQVDLTPKKATQNQVAETQVEVAQPRTASESKPRVTRSADVAEAKEASNAKVETGTDVTSKVTVEIGSIEGHNNTNKVEPHAGQRAVLKYKLKFENGLHQGDYFDFTLSNNVNTHGVSTARKVPEIKNGSVVMATGEVLEGGKIRYTFTNDIEDKVDVTAELEINLFIDPKTVQTNGNQTITSTLNEEQTSKELDVKYKDGIGNYYANLNGSIETFNKANNRFSHVAFIKPNNGKTTSVTVTGTLMKGSNQNGNQPKVRIFEYLGNNEDIAKSVYANTTDTSKFKEVTSNMSGNLNLQNNGSYSLNIENLDKTYVVHYDGEYLNGTDEVDFRTQMVGHPEQLYKYYYDRGYTLTWDNGLVLYSNKANGNEKNGPIIQNNKFEYKEDTIKETLTGQYDKNLVTTVEEEYDSSTLDIDYHTAIDGGGGYVDGYIETIEETDSSAIDIDYHTAVDSEAGHVGGYTESSEESNPIDFEESTHENSKHHADVVEYEEDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTVEDTKEYTTESNLIELVDELPEEHGQAQGPVEEITKNNHHISHSGLGTENGHGNYDVIEEIEENSHVDIKSELGYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNKGNQSFEEDTEKDKPKYEHGGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPSYQFGGHNSVDFEEDTLPKVSGQNEGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPAEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVAPTKKPQSKKSELPETGGEESTNKGMLFGGLFSILGLALLRRNKKNHKA mint q01924_strpy Sfb protein protein strpy Streptococcus pyogenes MNNKMFLNKEAGFLAHTKRKRRFAVTLVGVFFMLLASAGAIGFGQVAYAADEKTVPHRVSQNPEFPWYGYDFYKGPYTRYHNLQLNLNGSKTYQAYCFNLKRFEPKKEGSYFPNWYKRWDGSEETFVKYADNPRKDNESSRVIDVELEKNILRVLYNGYPNNGNGIMEGLEPLNAILVTQNAVWYYSDNSSIFNTDNFFTTEAKDLNIKPEQLSLMRVALKKLIDPKLSEESLKPVPSTFRLNIFESQDKLYQNLLSAEFVPENPPKPGETPEHGPKTPELDGTPIPEGPQRPNESLEPTLPPVMLDGQEVPEVPSESLEPALPPLMPELDGQEVPEVPSESLEPALPPLMPELDGQEVPEKPSVDLPIEDPRYEFNNKDQSPLAGESGETEYITEVYGNQQNPVDIDKKLPNETGFSGNMVETEDTKEPGVLMGGQSESVEFTKDTQTGMSGQTTPQVETEDTKEPGVLMGGQSESVEFTKDTQTGMSGQTASQVETEDTKEPGVLMGGQSESVEFTKDTQTGMSGQTTPQVETEDTKEPGVLMGGQSESVEFTKDTQTGMSGFSETVTIVEDTRPKLVFHFDNNEPKVEENREKPTKNITPILPATGDIENVLAFLGILILSVLPIFSLLKKQTKQ a0a459ndx2_lismn acrIIA2 D4271_13320 D4D89_13255 B1N40_08540 B4X79_13415 E0I39_13150 FJU19_12475 G3R95_002503 AF006_07510 APD94_09090 D4C60_14490 D4D22_14105 D5M63_13385 E1V33_15495 protein protein lismn Listeria monocytogenes MTLTRAQKKYAEAMHEFINMVDDFEESTPDFAKEVLHDSDYVVITKNEKYAVALCSLSTDECEYDTNLYLDEKLVDYSTVDVNGVTYYINIVETNDIDDLEIATDEDEMKSGNQEIILKSELK cas9_strp1 CRISPR-associated endonuclease Cas9/Csn1 cas9 csn1 SPy_1046 SpCas9 SpyCas9 protein protein strp1 Streptococcus pyogenes serotype M1 MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD a0a247d711_lismn acrIIA4 G3O21_003035 APD94_15065 GON91_02470 UI29_15095 protein protein lismn Listeria monocytogenes MNINDLIREIKNKDYTVKLSGTDSNSITQLIIRVNNDGNEYVISESENESIVEKFISAFKNGWNQEYEDEEEFYNDMQTITLKSELN urs000080e253_strpy_grna URS000080E253 gRNA grna guide rna guide RNA grna strpy Streptococcus pyogenes GGAAAUUAGGUGCGCUUGGCGUUUUAGAGCUAGAAAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUU eatx_strpy-ztox_strpy-1 3223909 N/A 3223911 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3223912 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false cas9-a0a247d711_lismn-2 3554925 N/A 3554929 predetermined predetermined participant predetermined unspecified role unspecified role ancillary ancillary in vitro translated in vitro translated protein in vitro translated in vitro In vitro N/A 3554930 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role bait bait purified purified gst tag gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3554931 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) physical association physical association false false false Extended Data Figure 1 cas9-a0a247d711_lismn-1 3554925 N/A 3554930 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role bait bait purified purified gst tag gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3554931 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) N/A 3554929 predetermined predetermined participant predetermined unspecified role unspecified role ancillary ancillary in vitro translated in vitro translated protein in vitro translated in vitro In vitro physical association physical association false false false Extended Data Figure 1 a0a247d711_lismn-cas9-3 3554925 N/A 3554930 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) N/A 3554931 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role bait bait purified purified A0A247D711:p.Asn12Thr N12T mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false A0A247D711:p.Gly38Ala G38A mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false gst tag gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false A0A247D711:p.Asp23Arg D23R mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3554929 predetermined predetermined participant predetermined unspecified role unspecified role ancillary ancillary in vitro translated in vitro translated protein in vitro translated in vitro In vitro physical association physical association false false false Extended Data Figure 3b (AcrIIA4 mutant) a0a247d711_lismn-cas9-4 3554925 N/A 3554931 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role bait bait purified purified A0A247D711:p.Asn36Tyr N36Y mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false gst tag gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false A0A247D711:p.Asn39Arg N39R mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false A0A247D711:p.Glu70Arg E70R mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false A0A247D711:p.Asp69Arg D69R mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false A0A247D711:p.[Asp14Arg;Glu40Arg] D14R, E40R mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false A0A247D711:p.[Asn36Arg;Asp69Arg] N36R, D69R mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3554930 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) N/A 3554929 predetermined predetermined participant predetermined unspecified role unspecified role ancillary ancillary in vitro translated in vitro translated protein in vitro translated in vitro In vitro physical association physical association false false false Extended Data Figure 3b (AcrIIA4 mutant) a0a247d711_lismn-cas9-5 3554925 N/A 3554930 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role prey prey purified purified Q99ZW2:p.[Arg976Tyr;Lys1200Tyr] R976Y, K1200Y mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q99ZW2:p.[Lys1200Tyr;Ser1216Arg] K1200Y,S1216R mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q99ZW2:p.Thr1138Tyr T1138Y mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false Q99ZW2:p.Asp1332Arg D1332R mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false Q99ZW2:p.Arg1335Tyr R1335Y mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false Q99ZW2:p.Ser1216Thr S1216T mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3554931 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role bait bait purified purified gst tag gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3554929 predetermined predetermined participant predetermined unspecified role unspecified role ancillary ancillary in vitro translated in vitro translated protein in vitro translated in vitro In vitro physical association physical association false false false Extended Data Figure 3b (SpyCas9 mutant) a0a394vx67_lismn-cas9-1 3554926 N/A 3554929 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component in vitro translated in vitro translated protein in vitro translated in vitro In vitro N/A 3554930 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) N/A 3554932 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) physical association physical association false false false Figure 4d a0a247d711_lismn-cas9-6 3554926 N/A 3554929 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component in vitro translated in vitro translated protein in vitro translated in vitro In vitro N/A 3554930 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) N/A 3554931 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) physical association physical association false false false Figure 4d a0a247d711_lismn-cas9-1 3554927 N/A 3554929 weight by bromide molecular weight estimation by bromide staining weight by bromide unspecified role unspecified role neutral component neutral component in vitro translated in vitro translated protein in vitro translated in vitro In vitro N/A 3554930 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) N/A 3554931 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) physical association physical association false false false Figure 1 a0a247d711_lismn-cas9-2 3554928 N/A 3554931 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3554930 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3554929 unspecified role unspecified role neutral component neutral component in vitro translated in vitro translated protein in vitro translated in vitro In vitro Stoichiometry: 1.0 physical association physical association false false false Figure 2, Extended Data Table 1 ifs-spn-1 3222327 N/A 3222331 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3222332 unspecified role unspecified role unspecified role unspecified role C-terminal glycohydrolase domain Region 194-454 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false spn-ifs-1 3222328 N/A 3222331 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3222332 unspecified role unspecified role bait bait C-terminal glycohydrolase domain Region 194-454 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false ifs-spn-2 3222329 N/A 3222331 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3222332 unspecified role unspecified role unspecified role unspecified role C-terminal glycohydrolase domain Region 194-454 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ifs-spn-3 3222330 N/A 3222331 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3222332 unspecified role unspecified role unspecified role unspecified role C-terminal glycohydrolase domain Region 194-454 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false fn1-fnbb-6 3472230 N/A 3472232 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris N/A 3472233 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false Fig 3 fn1-fnba-5 3472230 N/A 3472234 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3472232 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction false false false Fig 3 fn1-fnba-3 3472230 N/A 3472234 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3472232 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction false false false Fig 3 fn1-fnba-4 3472230 N/A 3472234 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3472232 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction false false false Fig 3 fn1-sfb-1 3472230 N/A 3472232 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris N/A 3472235 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false Fig 3 fn1-sfb-3 3472230 N/A 3472232 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris N/A 3472235 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false Fig 3 fn1-sfb-4 3472230 N/A 3472232 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris N/A 3472235 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false Fig 3 fn1-sfb-5 3472230 N/A 3472232 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris N/A 3472235 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false Fig 3 fn1-sfb-6 3472230 N/A 3472232 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris N/A 3472235 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false Fig 3 IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ sanderson_s-2006-1 Divergence in the plasminogen-binding group a streptococcal M protein family: functional conservation of binding site and potential role for immune selection of variants. in vitro In vitro competition binding competition binding predetermined predetermined participant predetermined Sanderson-Smith M., Batzloff M., Sriprakash KS., Dowton M., Ranson M., Walker MJ. BioCreative - Critical Assessment of Information Extraction systems in Biology Assays were carried out in the presence or absence of 50 fold molar excess of unlabelled Glu-plasminogen (UniProt accession number Q5TEH4) or unlabelled fluid phase PAM(NS13) (UniProt accession number Q6V4L5). mwalker@uow.edu.au J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation sanderson_s-2006-2 Divergence in the plasminogen-binding group a streptococcal M protein family: functional conservation of binding site and potential role for immune selection of variants. in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay predetermined predetermined participant predetermined Sanderson-Smith M., Batzloff M., Sriprakash KS., Dowton M., Ranson M., Walker MJ. BioCreative - Critical Assessment of Information Extraction systems in Biology Assays were carried out in the presence or absence of 50 fold molar excess of unlabelled Glu-plasminogen (UniProt accession number Q5TEH4) or unlabelled fluid phase PAM(NS13) (UniProt accession number Q6V4L5). mwalker@uow.edu.au J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation jenkins-2006-1 Human C4b-binding protein, structural basis for interaction with streptococcal M protein, a major bacterial virulence factor. in vitro In vitro saturation binding saturation binding immunostaining immunostaining Jenkins HT., Mark L., Ball G., Persson J., Lindahl G., Uhrin D., Blom AM., Barlow PN. BioCreative - Critical Assessment of Information Extraction systems in Biology Paul.Barlow@ed.ac.uk J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation jenkins-2006-2 Human C4b-binding protein, structural basis for interaction with streptococcal M protein, a major bacterial virulence factor. in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Jenkins HT., Mark L., Ball G., Persson J., Lindahl G., Uhrin D., Blom AM., Barlow PN. BioCreative - Critical Assessment of Information Extraction systems in Biology Paul.Barlow@ed.ac.uk J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation jenkins-2006-3 Human C4b-binding protein, structural basis for interaction with streptococcal M protein, a major bacterial virulence factor. in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Jenkins HT., Mark L., Ball G., Persson J., Lindahl G., Uhrin D., Blom AM., Barlow PN. BioCreative - Critical Assessment of Information Extraction systems in Biology NMR spectra were acquired (37 �C) on Bruker AVANCE 600 and 800 MHz spectrometers. Paul.Barlow@ed.ac.uk J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation arp4_strpy IgA receptor arp4 protein protein strpy Streptococcus pyogenes MARKDTNKQYSLRKLKTGTASVAVAVAVLGAGFANQTEVKAAEIKKPQADSAWNWPKEYNALLKENEELKVEREKYLSYADDKEKDPQYRALMGENQDLRKREGQYQDKIEELEKERKEKQERQEQLERQYQIEADKHYQEQQKKHQQEQQQLEAEKQKLAKDKQISDASRQGLSRDLEASRAAKKELEAEHQKLKEEKQISDASRQGLSRDLEASREAKKKVEADLAALTAEHQKLKEDKQISDASRQGLSRDLEASREAKKKVEADLAEANSKLQALEKLNKELEEGKKLSEKEKAELQARLEAEAKALKEQLAKQAEELAKLKGNQTPNAKVAPQANRSRSAMTQQKRTLPSTGETANPFFTAAAATVMVSAGMLALKRKEEN c4bpa_human C4b-binding protein alpha chain Proline-rich protein C4BPA C4BP protein protein human Homo sapiens Human MHPPKTPSGALHRKRKMAAWPFSRLWKVSDPILFQMTLIAALLPAVLGNCGPPPTLSFAAPMDITLTETRFKTGTTLKYTCLPGYVRSHSTQTLTCNSDGEWVYNTFCIYKRCRHPGELRNGQVEIKTDLSFGSQIEFSCSEGFFLIGSTTSRCEVQDRGVGWSHPLPQCEIVKCKPPPDIRNGRHSGEENFYAYGFSVTYSCDPRFSLLGHASISCTVENETIGVWRPSPPTCEKITCRKPDVSHGEMVSGFGPIYNYKDTIVFKCQKGFVLRGSSVIHCDADSKWNPSPPACEPNSCINLPDIPHASWETYPRPTKEDVYVVGTVLRYRCHPGYKPTTDEPTTVICQKNLRWTPYQGCEALCCPEPKLNNGEITQHRKSRPANHCVYFYGDEISFSCHETSRFSAICQGDGTWSPRTPSCGDICNFPPKIAHGHYKQSSSYSFFKEEIIYECDKGYILVGQAKLSCSYSHWSAPAPQCKALCRKPELVNGRLSVDKDQYVEPENVTIQCDSGYGVVGPQSITCSGNRTWYPEVPKCEWETPEGCEQVLTGKRLMQCLPNPEDVKMALEVYKLSLEIEQLELQRDSARQSTLDKEL q6v4k9_strpy protein protein strpy Streptococcus pyogenes RKLKTGTASVAVALTVVGAGLASQTEVKADQPVDHHRYTEANNAVLQGRTVSARALLHEINKNGQLRSENEELKADLQKKEQELKNLNDDVKKLNDEVALERLKNERHVHDEEVELERLKNERHDHDKKEAERKALEDKLADKQEHLDGALRYINEKEAERKEKEAEQKKLKEEKQISDASRQGLRRDLDASREAKKQVEKALEEANSKLAALEKLNKELEESKKLTEKEKAELQAKLEAEAKALKEKLAKQAEELAKLRAEKASDSQTPDAKPGNKAVPGKGQAPQAGTKPNQNKAPMKETKRQLPSTG q6v4l1_strpy protein protein strpy Streptococcus pyogenes RKLKTGTASVAVALTVVGAGLASQTEVKAENHPGYTAAQNGVLSELPGQAQAFSRAFLHEREKNGELRLENEGLKTDLKKKEQELESLKGDVEKLKDEVALERLKNERHVHDEEAELERLKNERYEHDKKEAERKALEDKLADKQEHLNGALRYINEKEAERKEKEAEQKKLKEEKQISDASRQGLRRDLDASREAKKQVEKDLANLTAELDKVKEEKQVSDASRQGLRRDLDASREAKKKVEADLAALTAEHQKLKEEKQISDASRQGLSRDLEASREAKKKVEADLAEANSKLQALEKLNKELEEGKKLSEKEKAELQARLEAEAKALKEQLAKQAEELAKLRAGKASDSQTPDAKPGNKAVPGKGQAPQAGTKPNQNKAPMKETKRQLPSTGETT q6v4l4_strpy protein protein strpy Streptococcus pyogenes RKLKTGTASVAVALTVVGAGLASQTEVKADQPVDHHRYTEANNAVLQGRTVSARALLHEINKNGQLRSENEELKADLQKKEQELKNLNDDVKKLNDEVALERLKNERHVHDEEVELERLKNERHDHDKKEAERKALEDKLADKQEHLDGALRYINEKEAERKEKEAEQKKLKEEKQISDASRQGLRRDLDASREAKKQVEKDLANLTAELDKAKEEKQISDASRQGLRRDLDASREAKKQVEKDLANLTAELDKVKEEKQISDASRQGLRRDLDASREAKKQVEKALEEANSKLAALEKLNKELEESKKLTEKEKAELQAKLEAEAKALKEKLAKQAEELAKLRAEKASDSQTPDAKPGNKAVPGKGQAPQAGTKPNQNKAPMKETKRQLPSTGETT q6v4l9_strpy protein protein strpy Streptococcus pyogenes RKLKTGTASVAVALTVLGAGFANQTEVKAADHPSYTAAKDEVLSKFSVPGHVWAHEREKNDKLSSENEGLKAGLQEKEQELKNLKADVEKLKDAAELERLKNERHDHDELERLKNERHDHDKREAERKALEDKLADKQEHLDGALRYINEKEAERKEKEAEQKKLKEEKQISDASRQGLRRDLDASREAKKQVEKDLANLTAELDKVKEEKQISDASRQGLRRDLDASREAKKQVEKDLADLTAELDKVKEEKQISDASRQGLRRDLDASREAKKQVEKALEEANSKLAALEKLNKELEESKKLTEKEKAELQAKLEAEAKALKEQLAKQAEELAKLRAEKASDSQTPDAKPGNKAVPGKGQAPQAGTKPNQNKAPMKETKRQLPSTGETT q6v4l5_strpy protein protein strpy Streptococcus pyogenes RKLKTGTASVAVALTVVGAGLASQTEVKANRADDARNEVLRGNLVRAELWYRQIQENDQLKLENKGLKTDLREKEEELQGLKDDVEKLTADAELQRLKNERHEEAELERLKSERHDHDKKEAERKALEDKLADKQEHLNGALRYINEKEAERKEKEAEQKKLKEEKQISDASRQGLRRDLDASREAKKQVEKDLANLTAELDKVKEEKQISDASRQGLRRDLDASREAKKQVEKGLANLTAELDKVKEEKQISDASRQGLRRDLDASREAKKQVEKALEEANSKLAALEKLNKELEESKKLTEKEKAELQAKLEAEAKALKEQLAKQAEELAKLRAEKASDSQTPDAKPGNKAVPGKGQAPQAGTKPNQNKAPMKETKRQLPSTSETT plmn_human Plasminogen PLG protein protein human Homo sapiens Human MEHKEVVLLLLLFLKSGQGEPLDDYVNTQGASLFSVTKKQLGAGSIEECAAKCEEDEEFTCRAFQYHSKEQQCVIMAENRKSSIIIRMRDVVLFEKKVYLSECKTGNGKNYRGTMSKTKNGITCQKWSSTSPHRPRFSPATHPSEGLEENYCRNPDNDPQGPWCYTTDPEKRYDYCDILECEEECMHCSGENYDGKISKTMSGLECQAWDSQSPHAHGYIPSKFPNKNLKKNYCRNPDRELRPWCFTTDPNKRWELCDIPRCTTPPPSSGPTYQCLKGTGENYRGNVAVTVSGHTCQHWSAQTPHTHNRTPENFPCKNLDENYCRNPDGKRAPWCHTTNSQVRWEYCKIPSCDSSPVSTEQLAPTAPPELTPVVQDCYHGDGQSYRGTSSTTTTGKKCQSWSSMTPHRHQKTPENYPNAGLTMNYCRNPDADKGPWCFTTDPSVRWEYCNLKKCSGTEASVVAPPPVVLLPDVETPSEEDCMFGNGKGYRGKRATTVTGTPCQDWAAQEPHRHSIFTPETNPRAGLEKNYCRNPDGDVGGPWCYTTNPRKLYDYCDVPQCAAPSFDCGKPQVEPKKCPGRVVGGCVAHPHSWPWQVSLRTRFGMHFCGGTLISPEWVLTAAHCLEKSPRPSSYKVILGAHQEVNLEPHVQEIEVSRLFLEPTRKDIALLKLSSPAVITDKVIPACLPSPNYVVADRTECFITGWGETQGTFGAGLLKEAQLPVIENKVCNRYEFLNGRVQSTELCAGHLAGGTDSCQGDSGGPLVCFEKDKYILQGVTSWGLGCARPNKPGVYVRVSRFVTWIEGVMRNN plg-q6v4l5 Competition binding assay reveals interaction between Plasminogen and Q6V4L5 504102 N/A 504104 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false glu N-terminal Glu residue experimental feature experimental feature n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 504105 unspecified role unspecified role bait bait c terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figure 3 Figure 4 Table 2 The circulating form of plasminogen was isolated from Human plasma which has a Glutamic acid residue attached to the N-termini of the plasminogen protein. Effective concentration of competitor (unlabelled Q6V4L5) required to inhibit binding by 50% (EC50) was 22.06 micro Molar. plg-q6v4l9 Competition binding assay reveals interaction between Plasminogen and Q6V4L9 504102 N/A 504104 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false glu N-terminal Glu residue experimental feature experimental feature n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 504106 unspecified role unspecified role bait bait c terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figure 3 Figure 4 Table 2 The circulating form of plasminogen was isolated from Human plasma which has a Glutamic acid residue attached to the N-termini of the plasminogen protein. Effective concentration of competitor (unlabelled Q6V4L9) required to inhibit binding by 50% (EC50) was 0.68 micro Molar. plg-q6v4l4 Competition binding assay reveals interaction between Plasminogen and Q6V4L4 504102 N/A 504107 unspecified role unspecified role bait bait c terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 504104 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false glu N-terminal Glu residue experimental feature experimental feature n-term range n-terminal range n-term range n-term range n-terminal range n-term range false direct interaction direct interaction false false false Figure 3 Figure 4 Table 2 The circulating form of plasminogen was isolated from Human plasma which has a Glutamic acid residue attached to the N-termini of the plasminogen protein. Effective concentration of competitor (unlabelled Q6V4L4) required to inhibit binding by 50% (EC50) was 1.96 micro Molar. plg-q6v4l1-1 Competition binding assay reveals interaction between Plasminogen and Q6V4L1 504102 N/A 504108 unspecified role unspecified role bait bait c terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 504104 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false glu N-terminal Glu residue experimental feature experimental feature n-term range n-terminal range n-term range n-term range n-terminal range n-term range false direct interaction direct interaction false false false Figure 3 Figure 4 Table 2 The circulating form of plasminogen was isolated from Human plasma which has a Glutamic acid residue attached to the N-termini of the plasminogen protein. Effective concentration of competitor (unlabelled Q6V4L1) required to inhibit binding by 50% (EC50) was 0.81 micro Molar. plg-q6v4k9 Competition binding assay reveals interaction between Plasminogen and Q6V4K9 504102 N/A 504104 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false glu N-terminal Glu residue experimental feature experimental feature n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 504109 unspecified role unspecified role bait bait c terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figure 3 Figure 4 Table 2 The circulating form of plasminogen was isolated from Human plasma which has a Glutamic acid residue attached to the N-termini of the plasminogen protein. Effective concentration of competitor (unlabelled Q6V4K9) required to inhibit binding by 50% (EC50) was 5.77 micro Molar. plg-q6v4l5-1 Ligand binding assay reveals interaction between Plasminogen and Q6V4L5 504103 N/A 504104 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false glu N-terminal Glu residue experimental feature experimental feature n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 504105 unspecified role unspecified role bait bait c terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figure 2C The circulating form of plasminogen was isolated from Human plasma which has a Glutamic acid residue attached to the N-termini of the plasminogen protein. plg-q6v4l9-1 Ligand binding assay reveals interaction between Plasminogen and Q6V4L9 504103 N/A 504106 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 504104 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false glu N-terminal Glu residue experimental feature experimental feature n-term range n-terminal range n-term range n-term range n-terminal range n-term range false direct interaction direct interaction false false false Figure 2C The circulating form of plasminogen was isolated from Human plasma which has a Glutamic acid residue attached to the N-termini of the plasminogen protein. plg-q6v4l1 Ligand binding assay reveals interaction between Plasminogen and Q6V4L1 504103 N/A 504108 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 504104 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false glu N-terminal Glu residue experimental feature experimental feature n-term range n-terminal range n-term range n-term range n-terminal range n-term range false direct interaction direct interaction false false false Figure 2C The circulating form of plasminogen was isolated from Human plasma which has a Glutamic acid residue attached to the N-termini of the plasminogen protein. plg-q6v4l4-1 Ligand binding assay reveals interaction between Plasminogen and Q6V4L4 504103 N/A 504107 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 504104 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false glu N-terminal Glu residue experimental feature experimental feature n-term range n-terminal range n-term range n-term range n-terminal range n-term range false direct interaction direct interaction false false false Figure 2C The circulating form of plasminogen was isolated from Human plasma which has a Glutamic acid residue attached to the N-termini of the plasminogen protein. apr4-c4bp Binding of C4BP and APR4 501868 N/A 501871 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) N/A 501872 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figure 1A apr4-c4bp-1 Binding of C4BP and APR4 501868 N/A 501872 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 501871 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figure 1A apr4-c4bp-2 Binding of C4BP and APR4 501868 N/A 501872 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) N/A 501871 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figure 1B Insertion of two alanine residues between Lysine 188 and Isoleucine 189 resulted in abolished interaction between the two proteins. apr4-c4bp-3 Binding of C4BP and APR4 501869 N/A 501872 unspecified role unspecified role neutral component neutral component c terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 501871 unspecified role unspecified role neutral component neutral component n terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 direct interaction direct interaction false false false Figure 1C A dimer of the APR4 protein was created as previous studies had shown that dimerization strongly enhanced C4BP binding. The APR4 protein used in this stidy also had a C-terminal Cys added. apr4-c4bp-4 Binding of C4BP and APR4 demonstrated by NMR 501870 N/A 501872 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 501871 unspecified role unspecified role neutral component neutral component n terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 direct interaction direct interaction false false false Figure 4 and Figure 5 The APR4 protein was present as a dimer. MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint loughman-2006-1 A novel adaptation of aldolase regulates virulence in Streptococcus pyogenes. strp1 Streptococcus pyogenes serotype M1 anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Loughman JA., Caparon MG. EMBO J. (0261-4189) 2006 2009-01-08 Only protein-protein interactions imex curation o85731_strpy ropB protein protein strpy Streptococcus pyogenes MEIGETVEFIRHSKNISIKQVCGDYLTRQTYYRFIKNNLDISSKKLLYILDNLNVNVDEFLFISNNFKQYKEFIDMDTAKHYFECRNIEGLNHILDSYKDSKSTKEKNLFALVKVLLATLTEEDCLTERTYLSNYLINIETWSHYETVLFNNCMFILESCFIEMVFSKVIVNLDKYNTLRYYGNESIRMFVNMLILFIQRQEYDKASEILAKIEDYQLNDDCLYERCCVSFFDGIIGLINGKEGAEQKCVQILEIFQLLNCKTIHHMFQTYLEAIKHKLS mint lacd1_strp1 Tagatose 1,6-diphosphate aldolase 1 SPy_1704 lacD1 lacD.1 M5005_Spy1395 Tagatose-bisphosphate aldolase 1 D-tagatose-1,6-bisphosphate aldolase 1 protein protein strp1 Streptococcus pyogenes serotype M1 MTITANKRHYLEKVSHQGIISALAFDQRGALKQMMAAHQEGEATVTQIETLKVLVSEELTPYASSILLDPEYGLLATKVRANQTGLLLAYEKTGYDATTTSRLPDCLVEWSVKRLKAAGADAIKFLLYYDVDGDEQINLQKQAYIERIGSECTAEDIPFFLELLSYDERISDNNSAAYAKLKPHKVNGAMSVFSDKRFGVDVLKVEVPVNMAYVEGFTEGEVHYSQAEAIKAFQDQEAASHLPYIYLSAGVSAKLFQETLYFAAAAGAQFSGVLCGRATWAGSVPVYITKGEDEARKWLCTEGFQNIDELNRVLEETASPWTEKI mint ropb-lacd1 568694 lacd1_strp1 568695 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell flag tagged flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 ropb 568696 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell ha tagged ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 physical association physical association false false false F3B mint