IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ waddington-2008-1 Adenovirus serotype 5 hexon mediates liver gene transfer. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Waddington SN., McVey JH., Bhella D., Parker AL., Barker K., Atoda H., Pink R., Buckley SM., Greig JA., Denby L., Custers J., Morita T., Francischetti IM., Monteiro RQ., Barouch DH., van Rooijen N., Napoli C., Havenga MJ., Nicklin SA., Baker AH. Source of FVII given in PMID: 16788098. Sequence of NAPc2 taken from PMID: 8700900, signal peptide sequence has been removed as described in the UniProt entry. Bait proteins attached to chip by amine coupling. Human and mouse X10 also interact with X-bp but this could not be matched to a single gene product. ab11f@clinmed.gla.ac.uk Cell (0092-8674) 2008 Only protein-protein interactions 6 imex curation capsh_ade05 Hexon protein L3 Protein II protein protein ade05 Human adenovirus C serotype 5 (HAdV-5) HAdV-5 Human adenovirus 5 MATPSMMPQWSYMHISGQDASEYLSPGLVQFARATETYFSLNNKFRNPTVAPTHDVTTDRSQRLTLRFIPVDREDTAYSYKARFTLAVGDNRVLDMASTYFDIRGVLDRGPTFKPYSGTAYNALAPKGAPNPCEWDEAATALEINLEEEDDDNEDEVDEQAEQQKTHVFGQAPYSGINITKEGIQIGVEGQTPKYADKTFQPEPQIGESQWYETEINHAAGRVLKKTTPMKPCYGSYAKPTNENGGQGILVKQQNGKLESQVEMQFFSTTEATAGNGDNLTPKVVLYSEDVDIETPDTHISYMPTIKEGNSRELMGQQSMPNRPNYIAFRDNFIGLMYYNSTGNMGVLAGQASQLNAVVDLQDRNTELSYQLLLDSIGDRTRYFSMWNQAVDSYDPDVRIIENHGTEDELPNYCFPLGGVINTETLTKVKPKTGQENGWEKDATEFSDKNEIRVGNNFAMEINLNANLWRNFLYSNIALYLPDKLKYSPSNVKISDNPNTYDYMNKRVVAPGLVDCYINLGARWSLDYMDNVNPFNHHRNAGLRYRSMLLGNGRYVPFHIQVPQKFFAIKNLLLLPGSYTYEWNFRKDVNMVLQSSLGNDLRVDGASIKFDSICLYATFFPMAHNTASTLEAMLRNDTNDQSFNDYLSAANMLYPIPANATNVPISIPSRNWAAFRGWAFTRLKTKETPSLGSGYDPYYTYSGSIPYLDGTFYLNHTFKKVAITFDSSVSWPGNDRLLTPNEFEIKRSVDGEGYNVAQCNMTKDWFLVQMLANYNIGYQGFYIPESYKDRMYSFFRNFQPMSRQVVDDTKYKDYQQVGILHQHNNSGFVGYLAPTMREGQAYPANFPYPLIGKTAVDSITQKKFLCDRTLWRIPFSSNFMSMGALTDLGQNLLYANSAHALDMTFEVDPMDEPTLLYVLFEVFDVVRVHRPHRGVIETVYLRTPFSAGNATT fa10_human Coagulation factor X Stuart factor Stuart-Prower factor F10 protein protein human Homo sapiens Human MGRPLHLVLLSASLAGLLLLGESLFIRREQANNILARVTRANSFLEEMKKGHLERECMEETCSYEEAREVFEDSDKTNEFWNKYKDGDQCETSPCQNQGKCKDGLGEYTCTCLEGFEGKNCELFTRKLCSLDNGDCDQFCHEEQNSVVCSCARGYTLADNGKACIPTGPYPCGKQTLERRKRSVAQATSSSGEAPDSITWKPYDAADLDPTENPFDLLDFNQTQPERGDNNLTRIVGGQECKDGECPWQALLINEENEGFCGGTILSEFYILTAAHCLYQAKRFKVRVGDRNTEQEEGGEAVHEVEVVIKHNRFTKETYDFDIAVLRLKTPITFRMNVAPACLPERDWAESTLMTQKTGIVSGFGRTHEKGRQSTRLKMLEVPYVDRNSCKLSSSFIITQNMFCAGYDTKQEDACQGDSGGPHVTRFKDTYFVTGIVSWGEGCARKGKYGIYTKVTAFLKWIDRSMKTRGLPKAKSHAPEVITSSPLK fa7_human Coagulation factor VII F7 Serum prothrombin conversion accelerator Proconvertin protein protein human Homo sapiens Human MVSQALRLLCLLLGLQGCLAAGGVAKASGGETRDMPWKPGPHRVFVTQEEAHGVLHRRRRANAFLEELRPGSLERECKEEQCSFEEAREIFKDAERTKLFWISYSDGDQCASSPCQNGGSCKDQLQSYICFCLPAFEGRNCETHKDDQLICVNENGGCEQYCSDHTGTKRSCRCHEGYSLLADGVSCTPTVEYPCGKIPILEKRNASKPQGRIVGGKVCPKGECPWQVLLLVNGAQLCGGTLINTIWVVSAAHCFDKIKNWRNLIAVLGEHDLSEHDGDEQSRRVAQVIIPSTYVPGTTNHDIALLRLHQPVVLTDHVVPLCLPERTFSERTLAFVRFSLVSGWGQLLDRGATALELMVLNVPRLMTQDCLQQSRKVGDSPNITEYMFCAGYSDGSKDSCKGDSGGPHATHYRGTWYLTGIVSWGQGCATVGHFGVYTRVSQYIEWLQKLMRSEPRPGVLLRAPFP q16938_ancca protein protein ancca Ancylostoma caninum (Dog hookworm) LVSYCSGKATMQCGENEKYDSCGSKECDKKCKYDGVEEEDDEEPNVPCLVRVCHQDCVCEEGFYRNKDDKCVSAEDCELDNMDFIYPGTRN fa10_mouse Coagulation factor X F10 Stuart factor protein protein mouse Mus musculus Mouse MGSPVQLSLLCVVLASLLLPGKGVFINRERANNVLARTRRANSFFEEFKKGNLERECMEEICSYEEVREIFEDDEKTKEYWTKYKDGDQCESSPCQNQGACRDGIGGYTCTCSEGFEGKNCELFVRKLCRLDNGDCDQFCREEQNSVVCSCASGYFLGNDGKSCISTAPFPCGKITTGRRKRSVALNTSDSELDLEDALLDEDFLSPTENPIELLNLNETQPERSSDDLVRIVGGRECKDGECPWQALLINEDNEGFCGGTILNEFYILTAAHCLHQARRFKVRVGDRNTEKEEGNEMVHEVDVVIKHNKFQRDTYDYDIAVLRLKTPITFRMNVAPACLPQKDWAESTLMTQKTGIVSGFGRTHEKGRQSNILKMLEVPYVDRNTCKLSTSFSITQNMFCAGYEAKLEDACQGDSGGPHVTRFKNTYYVTGIVSWGEGCARKGKYGIYTKVTTFLKWIDRSMKARVGPTAETPRTAGPPN q964q0_ixosc protein protein ixosc Ixodes scapularis (Black-legged tick) Deer tick MRAVSCFLYYGVAWIALGSWGASSSAERVSEMDIYEFESWVSCLDPEQVTCESQEGTHASYNRKTGQCEEQKGTECGGGENHFETLLKCNESCNDAPKPPCSLEVDYGVGRANIPRWYYDTNNATCEMFTYGGITGNKNNFESEEECKETCKGFSLLKKVNVTIN f10-pii-1 Interaction between Viral hexon protein and human F10 identified by SPR 728956 N/A 728957 unspecified role unspecified role bait bait N/A 728958 unspecified role unspecified role prey prey direct interaction direct interaction false false false Fig. 2D, Supp. Fig. 6 P04133 purified Association rate constant = 2.28E6 (1/Ms), dissociation rate constant = 4.5E-3 (1/s) Interaction was calcium-dependent. f7-pii-1 Interaction between Viral hexon protein and human F7 identified by SPR 728956 N/A 728959 unspecified role unspecified role prey prey N/A 728957 unspecified role unspecified role bait bait direct interaction direct interaction false false false Supp. Fig. 6 P04133 purified Interaction was calcium dependent. f10-napc2-1 Interaction between hookworm Napc2 and human F10 identified by SPR 728956 N/A 728960 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 728958 unspecified role unspecified role bait bait direct interaction direct interaction false false false Fig. 5 B and Supp. Table 1 Association rate constant = 8.6E6 (1/Ms), dissociation rate constant = 6.23E-4 (1/s) f10-napc2-2 Interaction between hookworm Napc2 and mouse F10 identified by SPR 728956 N/A 728960 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 728961 unspecified role unspecified role bait bait direct interaction direct interaction false false false Supp. Table 1 Association rate constant = 1.8E7 (1/Ms), dissociation rate constant = 3.1E-1 (1/s) f10-ixolaris-1 Interaction between tick ixolaris and human F10 identified by SPR 728956 N/A 728958 unspecified role unspecified role bait bait N/A 728962 unspecified role unspecified role prey prey direct interaction direct interaction false false false Fig. 5B and Supp. Table 1 Association rate constant = 1.35E5 (1/Ms), dissociation rate constant = 1.75E-4 (1/s) f10-ixolaris-2 Interaction between tick ixolaris and mouse F10 identified by SPR 728956 N/A 728961 unspecified role unspecified role bait bait N/A 728962 unspecified role unspecified role prey prey direct interaction direct interaction false false false Supp. Table 1 Association rate constant = 1.57E6 (1/Ms), dissociation rate constant = 8.1E-7 (1/s)