IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ kanduc-2002-2 Translational regulation of human papillomavirus type 16 E7 mRNA by the peptide SEQIKA, shared by rabbit alpha(1)-globin and human cytokeratin 7. in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa tag visualisation tag visualisation Kanduc D. In the RNA EMSA (REMSA) the 20-mer e7 mRNA was radiolabelled with 32-P. In the peptide EMSA (PEMSA) the unlabeled 20-mer e7 mRNA was incubated with biotinylated peptide. d.kanduc@biologia.uniba.it J. Virol. (0022-538X) 2002 hba_pig Hemoglobin subunit alpha HBA Hemoglobin alpha chain Alpha-globin protein protein pig Sus scrofa (Pig) Pig VLSAADKANVKAAWGKVGGQAGAHGAEALERMFLGFPTTKTYFPHFNLSHGSDQVKAHGQKVADALTKAVGHLDDLPGALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHHPDDFNPSVHASLDKFLANVSTVLTSKYR hba_colli Hemoglobin subunit alpha-A HBAA Hemoglobin alpha-A chain Alpha-A-globin protein protein colli Columba livia (Domestic pigeon) MVLSANDKSNVKAVFAKIGGQAGDLGGEALERLFITYPQTKTYFPHFDLSHGSAQIKGHGKKVAEALVEAANHIDDIAGALSKLSDLHAQKLRVDPVNFKLLGHCFLVVVAVHFPSLLTPEVHASLDKFVLAVGTVLTAKYR hba_rat Hemoglobin subunit alpha-1/2 Hba1 Hba-a1 Hemoglobin alpha-1/2 chain Alpha-1/2-globin protein protein rat Rattus norvegicus (Rat) Rat MVLSADDKTNIKNCWGKIGGHGGEYGEEALQRMFAAFPTTKTYFSHIDVSPGSAQVKAHGKKVADALAKAADHVEDLPGALSTLSDLHAHKLRVDPVNFKFLSHCLLVTLACHHPGDFTPAMHASLDKFLASVSTVLTSKYR hba_sheep Hemoglobin subunit alpha-1/2 Hemoglobin alpha-1/2 chain Alpha-1/2-globin protein protein sheep Ovis aries (Sheep) Sheep MVLSAADKSNVKAAWGKVGGNAGAYGAEALERMFLSFPTTKTYFPHFDLSHGSAQVKGHGEKVAAALTKAVGHLDDLPGTLSDLSDLHAHKLRVDPVNFKLLSHSLLVTLACHLPNDFTPAVHASLDKFLANVSTVLTSKYR hba_human Hemoglobin subunit alpha Hemoglobin alpha chain Alpha-globin HBA1 HBA2 protein protein human Homo sapiens Human MVLSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHFDLSHGSAQVKGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTPAVHASLDKFLASVSTVLTSKYR hba_bovin Hemoglobin subunit alpha HBA Hemoglobin alpha chain Alpha-globin protein protein bovin Bos taurus (Bovine) Bovine MVLSAADKGNVKAAWGKVGGHAAEYGAEALERMFLSFPTTKTYFPHFDLSHGSAQVKGHGAKVAAALTKAVEHLDDLPGALSELSDLHAHKLRVDPVNFKLLSHSLLVTLASHLPSDFTPAVHASLDKFLANVSTVLTSKYR 20-mer e7 mrna messenger rna mRNA mrna hpv16 Human papillomavirus type 16 TTATGGTTTCTGAGAACAGA A 20-mer oligonucleotide complementary to the 3' end of HPV16 E7 mRNA hba_rabit Hemoglobin subunit alpha-1/2 Hemoglobin alpha-1/2 chain Alpha-1/2-globin protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MVLSPADKTNIKTAWEKIGSHGGEYGAEAVERMFLGFPTTKTYFPHFDFTHGSEQIKAHGKKVSEALTKAVGHLDDLPGALSTLSDLHAHKLRVDPVNFKLLSHCLLVTLANHHPSEFTPAVHASLDKFLANVSTVLTSKYR hpv16e7-sxqxkx RNA EMSA to show interaction between hpv16 e7 and SXQXKX peptide fragment in different hemaglobin 666545 N/A 666548 unspecified role unspecified role bait bait N/A 666549 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro physical association physical association false false false Table 2, Figure 7A, 7B Rabbit {alpha}1-globin 53-58 SEQIKA motif showed binding to radiolabeled HPV16 E7 mRNA by gel retardation experiments and this binding was suppressed by anti-p32Pabs. hpv16e7-sxqxkx-1 RNA EMSA to show interaction between hpv16 e7 and SXQXKX peptide fragment in different hemaglobin 666545 N/A 666550 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 666548 unspecified role unspecified role bait bait physical association physical association false false false Table 2, Figure 7A, 7B Human {alpha}1-globin 53-58 SAQIKG motif showed binding to radiolabeled HPV16 E7 mRNA by gel retardation experiments and this binding was suppressed by anti-p32Pabs. hpv16e7-sxqxkx-2 RNA EMSA to show interaction between hpv16 e7 and SXQXKX peptide fragment in different hemaglobin 666545 N/A 666551 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 666548 unspecified role unspecified role bait bait physical association physical association false false false Table 2, Figure 7A, 7B Bovine {alpha}1-globin 53-58 SAQVKG motif showed binding to radiolabeled HPV16 E7 mRNA by gel retardation experiments and this binding was suppressed by anti-p32Pabs. hpv16e7-sxqxkx-3 RNA EMSA to show interaction between hpv16 e7 and SXQXKX peptide fragment in different hemaglobin 666545 N/A 666552 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 666548 unspecified role unspecified role bait bait physical association physical association false false false Table 2, Figure 7A, 7B Rat {alpha}1-globin 53-58 SAQVKA motif showed binding to radiolabeled HPV16 E7 mRNA by gel retardation experiments and this binding was suppressed by anti-p32Pabs. hpv16e7-sxqxkx-4 RNA EMSA to show interaction between hpv16 e7 and SXQXKX peptide fragment in different hemaglobin 666545 N/A 666548 unspecified role unspecified role bait bait N/A 666553 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro physical association physical association false false false Table 2, Figure 7A, 7B Sheep {alpha}1-globin 53-58 SAQVKG motif showed binding to radiolabeled HPV16 E7 mRNA by gel retardation experiments and this binding was suppressed by anti-p32Pabs. hpv16e7-sxqxkx-5 RNA EMSA to show interaction between hpv16 e7 and SXQXKX peptide fragment in different hemaglobin 666545 N/A 666548 unspecified role unspecified role bait bait N/A 666554 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro physical association physical association false false false Table 2, Figure 7A, 7B Sheep {alpha}1-globin 52-57 SDQVKA motif showed binding to radiolabeled HPV16 E7 mRNA by gel retardation experiments and this binding was suppressed by anti-p32Pabs. hpv16e7-sxqxkx-6 RNA EMSA to show interaction between hpv16 e7 and SXQXKX peptide fragment in different hemaglobin 666545 N/A 666555 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 666548 unspecified role unspecified role bait bait physical association physical association false false false Table 2, Figure 7A, 7B Pigeon {alpha}1-globin 53-58 SAQIKG motif showed binding to radiolabeled HPV16 E7 mRNA by gel retardation experiments and this binding was suppressed by anti-p32Pabs.