DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ bondy_denomy-2015-2 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Bondy-Denomy J., Garcia B., Strum S., Du M., Rollins MF., Hidalgo-Reyes Y., Wiedenheft B., Maxwell KL., Davidson AR. DIP original experiment: DIP-267058X Nature 2015 IMEx Multiple mechanisms for CRISPR-Cas inhibition by anti-CRISPR proteins. Bondy-Denomy J., Garcia B., Strum S., Du M., Rollins MF., Hidalgo-Reyes Y., Wiedenheft B., Maxwell KL., Davidson AR. Only protein-protein interactions imex curation bondy_denomy-2015-7 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Bondy-Denomy J., Garcia B., Strum S., Du M., Rollins MF., Hidalgo-Reyes Y., Wiedenheft B., Maxwell KL., Davidson AR. DIP original experiment: DIP-267062X Nature 2015 IMEx Multiple mechanisms for CRISPR-Cas inhibition by anti-CRISPR proteins. Bondy-Denomy J., Garcia B., Strum S., Du M., Rollins MF., Hidalgo-Reyes Y., Wiedenheft B., Maxwell KL., Davidson AR. Only protein-protein interactions imex curation bondy_denomy-2015-8 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Bondy-Denomy J., Garcia B., Strum S., Du M., Rollins MF., Hidalgo-Reyes Y., Wiedenheft B., Maxwell KL., Davidson AR. DIP original experiment: DIP-267065X Nature 2015 IMEx Multiple mechanisms for CRISPR-Cas inhibition by anti-CRISPR proteins. Bondy-Denomy J., Garcia B., Strum S., Du M., Rollins MF., Hidalgo-Reyes Y., Wiedenheft B., Maxwell KL., Davidson AR. Only protein-protein interactions imex curation bondy_denomy-2015-9 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight silver stain molecular weight estimation by silver staining weight silver stain Bondy-Denomy J., Garcia B., Strum S., Du M., Rollins MF., Hidalgo-Reyes Y., Wiedenheft B., Maxwell KL., Davidson AR. DIP original experiment: DIP-267060X Nature 2015 IMEx Multiple mechanisms for CRISPR-Cas inhibition by anti-CRISPR proteins. Bondy-Denomy J., Garcia B., Strum S., Du M., Rollins MF., Hidalgo-Reyes Y., Wiedenheft B., Maxwell KL., Davidson AR. Only protein-protein interactions imex curation bondy_denomy-2015-13 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Bondy-Denomy J., Garcia B., Strum S., Du M., Rollins MF., Hidalgo-Reyes Y., Wiedenheft B., Maxwell KL., Davidson AR. DIP original experiment: DIP-267067X Nature 2015 IMEx Multiple mechanisms for CRISPR-Cas inhibition by anti-CRISPR proteins. Bondy-Denomy J., Garcia B., Strum S., Du M., Rollins MF., Hidalgo-Reyes Y., Wiedenheft B., Maxwell KL., Davidson AR. Only protein-protein interactions imex curation cas6_pseab CRISPR-associated endonuclease Cas6/Csy4 cas6f csy4 PA14_33300 protein protein pseab Pseudomonas aeruginosa (strain UCBPP-PA14) MDHYLDIRLRPDPEFPPAQLMSVLFGKLHQALVAQGGDRIGVSFPDLDESRSRLGERLRIHASADDLRALLARPWLEGLRDHLQFGEPAVVPHPTPYRQVSRVQAKSNPERLRRRLMRRHDLSEEEARKRIPDTVARALDLPFVTLRSQSTGQHFRLFIRHGPLQVTAEEGGFTCYGLSKGGFVPWF csy1_pseab CRISPR-associated protein Csy1 csy1 PA14_33330 protein protein pseab Pseudomonas aeruginosa (strain UCBPP-PA14) MTSPLPTPTWQELRQFIESFIQERLQGKLDKLQPDEDDKRQTLLATHRREAWLADAARRVGQLQLVTHTLKPIHPDARGSNLHSLPQAPGQPGLAGSHELGDRLVSDVVGNAAALDVFKFLSLQYQGKNLLNWLTEDSAEALQALSDNAEQAREWRQAFIGITTVKGAPASHSLAKQLYFPLPGSGYHLLAPLFPTSLVHHVHALLREARFGDAAKAAREARSRQESWPHGFSEYPNLAIQKFGGTKPQNISQLNNERRGENWLLPSLPPNWQRQNVNAPMRHSSVFEHDFGRTPEVSRLTRTLQRFLAKTVHNNLAIRQRRAQLVAQICDEALQYAARLRELEPGWSATPGCQLHDAEQLWLDPLRAQTDETFLQRRLRGDWPAEVGNRFANWLNRAVSSDSQILGSPEAAQWSQELSKELTMFKEILEDERD acr30_bpd31 Anti-CRISPR protein 30 orf30 Gene product 30 protein protein bpd31 Pseudomonas phage D3112 Bacteriophage D3112 MIAQQHKDTVAACEAAEAIAIAKDQVWDGEGYTKYTFDDNSVLIQSGTTQYAMDADDADSIKGYADWLDDEARSAEASEIERLLESVEEE csy3_pseab CRISPR-associated protein Csy3 csy3 csy1-3 PA14_33310 protein protein pseab Pseudomonas aeruginosa (strain UCBPP-PA14) MSKPILSTASVLAFERKLDPSDALMSAGAWAQRDASQEWPAVTVREKSVRGTISNRLKTKDRDPAKLDASIQSPNLQTVDVANLPSDADTLKVRFTLRVLGGAGTPSACNDAAYRDKLLQTVATYVNDQGFAELARRYAHNLANARFLWRNRVGAEAVEVRINHIRQGEVARAWRFDALAIGLRDFKADAELDALAELIASGLSGSGHVLLEVVAFARIGDGQEVFPSQELILDKGDKKGQKSKTLYSVRDAAAIHSQKIGNALRTIDTWYPDEDGLGPIAVEPYGSVTSQGKAYRQPKQKLDFYTLLDNWVLRDEAPAVEQQHYVIANLIRGGVFGEAEEK csy2_pseab CRISPR-associated protein Csy2 csy2 PA14_33320 protein protein pseab Pseudomonas aeruginosa (strain UCBPP-PA14) MSVTDPEALLLLPRLSIQNANAISSPLTWGFPSPGAFTGFVHALQRRVGISLDIELDGVGIVCHRFEAQISQPAGKRTKVFNLTRNPLNRDGSTAAIVEEGRAHLEVSLLLGVHGDGLDDHPAQEIARQVQEQAGAMRLAGGSILPWCNERFPAPNAELLMLGGSDEQRRKNQRRLTRRLLPGFALVSREALLQQHLETLRTTLPEATTLDALLDLCRINFEPPATSSEEEASPPDAAWQVRDKPGWLVPIPAGYNALSPLYLPGEVRNARDRETPLRFVENLFGLGEWLSPHRVAALSDLLWYHHAEPDKGLYRWSTPRFVEHAIA l7p7m1_9caud JBD30_035 protein protein pseudomonas phage jbd30 Pseudomonas phage JBD30 MKFIKYLSTAHLNYMNIAVYENGSKIKARVENVVNGKSVGARDFDSTEQLESWFYGLPGSGLGRIENAMNEISRRENP csy1-csy2-2 3284377 N/A 3284395 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3284393 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3284394 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false cas6f-csy1-9 3284382 N/A 3284393 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3284394 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3284395 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3284396 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3284391 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3284392 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false csy1-csy2-5 3284383 N/A 3284395 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3284392 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3284393 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3284394 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false cas6f-csy1-10 3284384 N/A 3284395 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3284391 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3284392 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3284393 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3284394 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false cas6f-csy1-12 3284388 N/A 3284395 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3284391 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3284392 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3284393 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3284394 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false