IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ el_kamand-2021-2 in vitro In vitro solution nmr solution state nmr solution state nmr solution nmr predetermined predetermined participant predetermined El-Kamand S., Du Plessis MD., Breen N., Johnson L., Beard S., Kwan AH., Richard DJ., Cubeddu L., Gamsjaeger R., El-Kamand S., Du Plessis MD., Breen N., Johnson L., Beard S., Kwan AH., Richard DJ., Cubeddu L., Gamsjaeger R. Virus - Publications including interactions involving viral proteins r.gamsjaeger@westernsydney.edu.au,l.cubeddu@westernsydney.edu.au Proteins 2021 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus el_kamand-2021-1 ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined El-Kamand S., Du Plessis MD., Breen N., Johnson L., Beard S., Kwan AH., Richard DJ., Cubeddu L., Gamsjaeger R., El-Kamand S., Du Plessis MD., Breen N., Johnson L., Beard S., Kwan AH., Richard DJ., Cubeddu L., Gamsjaeger R. Virus - Publications including interactions involving viral proteins r.gamsjaeger@westernsydney.edu.au,l.cubeddu@westernsydney.edu.au Proteins 2021 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus el_kamand-2021-3 in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined El-Kamand S., Du Plessis MD., Breen N., Johnson L., Beard S., Kwan AH., Richard DJ., Cubeddu L., Gamsjaeger R., El-Kamand S., Du Plessis MD., Breen N., Johnson L., Beard S., Kwan AH., Richard DJ., Cubeddu L., Gamsjaeger R. Virus - Publications including interactions involving viral proteins r.gamsjaeger@westernsydney.edu.au,l.cubeddu@westernsydney.edu.au Proteins 2021 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus ssdna_oligo (thymidine x23) ss dna single stranded deoxyribonucleic acid ss DNA ss dna chemical synthesis Chemical synthesis (Chemical synthesis) TTTTTTTTTTTTTTTTTTTTTTT p0dtd1-pro_0000449627 Non-structural protein 9 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV NNELSPVALRQMSCAAGTTQTACTDDNALAYYNTTKGGRFVLALLSDLQDLKWARFPKSDGTGTIYTELEPPCRFVTDTPKGPKVKYLYFIKGLNNLNRGMVLGSLAATVRLQ 4141 4253 ssrna_oligo (uracilx23) rna ribonucleic acid RNA rna chemical synthesis Chemical synthesis (Chemical synthesis) UUUUUUUUUUUUUUUUUUUUUUU rep-267 4078675 N/A 4078678 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) N/A 4078679 unspecified role unspecified role neutral component neutral component purified purified region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 4, Supp. figure 1 rep-ssdna_oligo (thymidine x23)-1 4078675 N/A 4078680 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) N/A 4078679 unspecified role unspecified role neutral component neutral component purified purified region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 4, Supp. figure 1 rep-266 4078676 N/A 4078679 unspecified role unspecified role neutral component neutral component purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 2.0 direct interaction direct interaction false false false 2 ssrna_oligo (uracilx23)-rep-1 4078677 N/A 4078680 unspecified role unspecified role bait bait 5' prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 4078679 unspecified role unspecified role prey prey purified purified P0DTD1-PRO_0000449627:p.Tyr66Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false P0DTD1-PRO_0000449627:p.Phe40Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P0DTD1-PRO_0000449627:p.Lys92Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P0DTD1-PRO_0000449627:p.[Arg55Ala;Lys92Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P0DTD1-PRO_0000449627:p.Arg55Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 4078677 7, Supp. figure 2 Kd >450 uM and >1000 uM for K92A and R55/K92A, respectively.