IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ hudk-2021-1 in vitro In vitro confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Hudák A., Letoha A., Szilák L., Letoha T. Virus - Publications including interactions involving viral proteins Syndecan transfectants were incubated with heat-inactivated SARS-CoV-2. tamas.letoha@pharmacoidea.eu International journal of molecular sciences 2021 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus hudk-2021-2 in vitro In vitro facs fluorescence-activated cell sorting FACS Flow cytometry facs antibody detection identification by antibody antibody detection Hudák A., Letoha A., Szilák L., Letoha T. Virus - Publications including interactions involving viral proteins Syndecan transfectants were incubated with heat-inactivated SARS-CoV-2. tamas.letoha@pharmacoidea.eu International journal of molecular sciences 2021 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus hudk-2021-3 in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hudák A., Letoha A., Szilák L., Letoha T. Virus - Publications including interactions involving viral proteins tamas.letoha@pharmacoidea.eu International journal of molecular sciences 2021 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus hudk-2021-4 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hudák A., Letoha A., Szilák L., Letoha T. Virus - Publications including interactions involving viral proteins tamas.letoha@pharmacoidea.eu International journal of molecular sciences 2021 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus sdc4_human Syndecan-4 SDC4 Amphiglycan Ryudocan core protein protein protein human Homo sapiens Human MAPARLFALLLFFVGGVAESIRETEVIDPQDLLEGRYFSGALPDDEDVVGPGQESDDFELSGSGDLDDLEDSMIGPEVVHPLVPLDNHIPERAGSGSQVPTEPKKLEENEVIPKRISPVEESEDVSNKVSMSSTVQGSNIFERTEVLAALIVGGIVGILFAVFLILLLMYRMKKKDEGSYDLGKKPIYKKAPTNEFYA spike_sars2 Spike glycoprotein S E2 Peplomer protein 2 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT ace2_human Angiotensin-converting enzyme 2 ACE2 UNQ868/PRO1885 Angiotensin-converting enzyme homolog Metalloprotease MPROT15 Angiotensin-converting enzyme-related carboxypeptidase protein protein human Homo sapiens Human MSSSSWLLLSLVAVTAAQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVSIWLIVFGVVMGVIVVGIVILIFTGIRDRKKKNKARSGENPYASIDISKGENNPGFQNTDDVQTSF mint Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses s-sdc4-1 4062546 N/A 4062550 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells N/A 4062551 unspecified role unspecified role neutral component neutral component cell lysate cell lysate conditioned medium conditioned medium chlae-vero_e6 chlae-vero_e6 cells vero_e6 vero_e6 cell African green monkey kidney epithelial cells epithelium Epithelium [epithelial cells] colocalization colocalization false false false 2A, Supp. figure 5. s-sdc4-3 4062546 N/A 4062550 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed location:juxtamembrane region of ecd gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false HSA sufficient to bind sufficient binding region sufficient to bind range range range range false CBD sufficient to bind sufficient binding region sufficient to bind range range range range false human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells N/A 4062551 unspecified role unspecified role neutral component neutral component cell lysate cell lysate conditioned medium conditioned medium chlae-vero_e6 chlae-vero_e6 cells vero_e6 vero_e6 cell African green monkey kidney epithelial cells epithelium Epithelium [epithelial cells] colocalization colocalization false false false 3E, supp. figure 7 Substantial colocalization of SARS-CoV-2 was seen with either the HSA (Heparan Sulfate attachment site) or CBD (Cell Binding Domain) mutants;sequences of these mutants cannot be traced. s-sdc4-5 4062546 N/A 4062550 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma N/A 4062551 unspecified role unspecified role neutral component neutral component cell lysate cell lysate conditioned medium conditioned medium chlae-vero_e6 chlae-vero_e6 cells vero_e6 vero_e6 cell African green monkey kidney epithelial cells epithelium Epithelium [epithelial cells] colocalization colocalization false false false 5B ace2-s-193 4062546 N/A 4062552 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma N/A 4062551 unspecified role unspecified role neutral component neutral component cell lysate cell lysate conditioned medium conditioned medium chlae-vero_e6 chlae-vero_e6 cells vero_e6 vero_e6 cell African green monkey kidney epithelial cells epithelium Epithelium [epithelial cells] colocalization colocalization false false false 5B s-sdc4-7 4062546 N/A 4062550 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed HSA sufficient to bind sufficient binding region sufficient to bind range range range range false CBD sufficient to bind sufficient binding region sufficient to bind range range range range false human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells N/A 4062551 unspecified role unspecified role neutral component neutral component purified purified S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) colocalization colocalization false false false 6E, (7D) It has been assumed that figure 7 results were obtained using K562 cells;however NO cell line appears to have been stated. Both the HSA (Heparan Sulfate attachment site) and CBD (Cell Binding Domain) domains proved to serve a significant role in interacting with spikeS1. s-sdc4-9 4062546 N/A 4062550 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma N/A 4062551 unspecified role unspecified role neutral component neutral component purified purified S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) colocalization colocalization false false false 8G-RHS s-sdc4-10 4062546 N/A 4062550 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma N/A 4062551 unspecified role unspecified role neutral component neutral component purified purified S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) colocalization colocalization false false false 8G -LHS s-sdc4-2 4062547 N/A 4062550 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells N/A 4062551 unspecified role unspecified role neutral component neutral component cell lysate cell lysate conditioned medium conditioned medium chlae-vero_e6 chlae-vero_e6 cells vero_e6 vero_e6 cell African green monkey kidney epithelial cells epithelium Epithelium [epithelial cells] colocalization colocalization false false false 2B s-sdc4-4 4062547 N/A 4062550 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed CBD sufficient to bind sufficient binding region sufficient to bind range range range range false location:juxtamembrane region of ecd gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false HSA sufficient to bind sufficient binding region sufficient to bind range range range range false human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells N/A 4062551 unspecified role unspecified role neutral component neutral component cell lysate cell lysate conditioned medium conditioned medium chlae-vero_e6 chlae-vero_e6 cells vero_e6 vero_e6 cell African green monkey kidney epithelial cells epithelium Epithelium [epithelial cells] colocalization colocalization false false false 3D Substantial colocalization of SARS-CoV-2 was seen with either the HSA (Heparan Sulfate attachment site) or CBD (Cell Binding Domain) mutants;sequences of these mutants cannot be traced. s-sdc4-6 4062547 N/A 4062550 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma N/A 4062551 unspecified role unspecified role neutral component neutral component cell lysate cell lysate conditioned medium conditioned medium chlae-vero_e6 chlae-vero_e6 cells vero_e6 vero_e6 cell African green monkey kidney epithelial cells epithelium Epithelium [epithelial cells] colocalization colocalization false false false 5A ace2-s-194 4062547 N/A 4062552 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma N/A 4062551 unspecified role unspecified role neutral component neutral component cell lysate cell lysate conditioned medium conditioned medium chlae-vero_e6 chlae-vero_e6 cells vero_e6 vero_e6 cell African green monkey kidney epithelial cells epithelium Epithelium [epithelial cells] colocalization colocalization false false false 5A s-sdc4-8 4062547 N/A 4062550 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed HSA sufficient to bind sufficient binding region sufficient to bind range range range range false CBD sufficient to bind sufficient binding region sufficient to bind range range range range false human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells N/A 4062551 unspecified role unspecified role neutral component neutral component purified purified S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) colocalization colocalization false false false 6D, (7C) Both the HSA (Heparan Sulfate attachment site) or CBD (Cell Binding Domain) domains proved to serve a significant role in interacting with spikeS1. It has been assumed that figure 7 results were obtained using K562 cells;however NO cell line appears to have been stated. s-sdc4-11 4062547 N/A 4062550 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma N/A 4062551 unspecified role unspecified role neutral component neutral component purified purified S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) colocalization colocalization false false false 8F-RHS s-sdc4-12 4062547 N/A 4062550 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma N/A 4062551 unspecified role unspecified role neutral component neutral component purified purified S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) colocalization colocalization false false false 8F -LHS s-sdc4-13 4062548 N/A 4062550 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma N/A 4062551 unspecified role unspecified role bait bait purified purified S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false 8H, lane 3 s-sdc4-14 4062548 N/A 4062550 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma N/A 4062551 unspecified role unspecified role bait bait purified purified S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false 8H, lane 2 s-sdc4-15 4062548 N/A 4062550 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma N/A 4062551 unspecified role unspecified role bait bait cell lysate cell lysate conditioned medium conditioned medium S1 region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chlae-vero_e6 chlae-vero_e6 cells vero_e6 vero_e6 cell African green monkey kidney epithelial cells epithelium Epithelium [epithelial cells] physical association physical association false false false Supp. figure 10 A (A549+SARS2) s-sdc4-16 4062548 N/A 4062550 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells N/A 4062551 unspecified role unspecified role bait bait cell lysate cell lysate conditioned medium conditioned medium S1 region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chlae-vero_e6 chlae-vero_e6 cells vero_e6 vero_e6 cell African green monkey kidney epithelial cells epithelium Epithelium [epithelial cells] physical association physical association false false false Supp. figure 10 A (SDC4+SARS2) s-sdc4-17 4062548 N/A 4062552 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma N/A 4062551 unspecified role unspecified role bait bait cell lysate cell lysate conditioned medium conditioned medium S1 region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chlae-vero_e6 chlae-vero_e6 cells vero_e6 vero_e6 cell African green monkey kidney epithelial cells epithelium Epithelium [epithelial cells] physical association physical association false false false Supp. figure 10 B (A549+SARS2) s-ace2-44 4062548 N/A 4062552 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells N/A 4062551 unspecified role unspecified role bait bait cell lysate cell lysate conditioned medium conditioned medium S1 region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chlae-vero_e6 chlae-vero_e6 cells vero_e6 vero_e6 cell African green monkey kidney epithelial cells epithelium Epithelium [epithelial cells] physical association physical association false false false Supp. figure 10 B (K562+SARS2) sdc4-s-1 4062549 N/A 4062550 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role bait bait over-expressed over expressed level over-expressed CBD sufficient to bind sufficient binding region sufficient to bind range range range range false HSA sufficient to bind sufficient binding region sufficient to bind range range range range false location:juxtamembrane region of ecd gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells N/A 4062551 unspecified role unspecified role prey prey purified purified S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false supp. figure 12 SDC4 CBD (Cell Binding Domain) or HSA (HS attachment site) domains can bind spikeS1. (exact sequences are not traceable). Only weak binding was seen.